##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937136_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2863793 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50540664077327 31.0 31.0 34.0 30.0 34.0 2 31.57531741993922 33.0 31.0 34.0 30.0 34.0 3 31.59361937123249 33.0 31.0 34.0 28.0 34.0 4 35.20808487205605 37.0 35.0 37.0 33.0 37.0 5 35.204437611237964 37.0 35.0 37.0 33.0 37.0 6 35.264458359944314 37.0 35.0 37.0 32.0 37.0 7 35.1955340347574 37.0 35.0 37.0 32.0 37.0 8 35.1583777179426 37.0 35.0 37.0 32.0 37.0 9 36.739777281388704 39.0 37.0 39.0 32.0 39.0 10-11 36.59270205632879 39.0 37.0 39.0 32.0 39.0 12-13 36.48990185393986 39.0 37.0 39.0 32.0 39.0 14-15 37.570666245779634 40.0 37.0 41.0 32.0 41.0 16-17 37.4934913242682 40.0 37.0 41.0 32.0 41.0 18-19 37.44817432684555 40.0 37.0 41.0 32.0 41.0 20-21 37.38389419207324 40.0 37.0 41.0 31.5 41.0 22-23 37.265887583355365 40.0 37.0 41.0 31.0 41.0 24-25 37.21470546230122 39.5 36.5 41.0 31.0 41.0 26-27 37.2391642831727 40.0 37.0 41.0 31.0 41.0 28-29 37.15918818154803 40.0 36.5 41.0 31.0 41.0 30-31 36.993304334496244 39.0 36.0 41.0 30.5 41.0 32-33 36.80431564013181 39.0 36.0 41.0 30.0 41.0 34-35 36.62881150977043 39.0 35.5 41.0 30.0 41.0 36-37 36.442214049688644 39.0 35.0 41.0 30.0 41.0 38-39 36.2812762654284 39.0 35.0 41.0 30.0 41.0 40-41 36.084290135495124 39.0 35.0 40.0 29.0 41.0 42-43 35.85662738193717 39.0 35.0 40.0 28.5 41.0 44-45 35.59158291119505 38.0 35.0 40.0 27.0 41.0 46-47 35.24220954517313 38.0 34.5 40.0 26.0 41.0 48-49 34.99094993946839 38.0 34.0 40.0 25.0 41.0 50-51 34.282729757353266 37.5 33.5 39.5 24.0 40.5 52-53 34.46054236461923 38.0 33.5 39.5 24.0 40.5 54-55 34.83947914531532 38.0 34.0 40.0 25.0 41.0 56-57 34.71458394513849 38.0 34.0 40.0 24.5 41.0 58-59 34.4826520282716 38.0 34.0 40.0 24.0 41.0 60-61 34.17976875423608 38.0 34.0 40.0 23.0 41.0 62-63 33.85841993468104 37.0 33.0 40.0 22.5 41.0 64-65 33.51373143938825 37.0 33.0 40.0 22.0 41.0 66-67 33.134239276372284 36.0 32.5 39.0 21.5 41.0 68-69 32.734535980777935 36.0 32.0 39.0 20.0 41.0 70-71 32.32187888579936 35.0 32.0 38.5 19.5 40.0 72-73 31.93212201440537 35.0 31.0 37.5 18.0 40.0 74-75 31.541530760079375 35.0 31.0 37.0 17.5 39.0 76-77 31.090646216399023 35.0 31.0 36.5 12.5 39.0 78-79 30.6408630093027 35.0 30.5 36.0 9.0 38.5 80-81 30.214241916227884 34.0 30.0 36.0 7.0 37.0 82-83 29.821809397536764 34.0 30.0 35.0 4.5 37.0 84-85 29.46004878844246 34.0 29.0 35.0 2.0 36.5 86-87 29.11137781257235 34.0 29.0 35.0 2.0 36.0 88-89 28.756809762437438 34.0 29.0 35.0 2.0 36.0 90-91 28.38735620905561 34.0 29.0 35.0 2.0 35.0 92-93 27.996215508592975 34.0 27.5 35.0 2.0 35.0 94-95 27.608388595125415 34.0 27.0 35.0 2.0 35.0 96-97 27.090824127302497 33.5 26.0 35.0 2.0 35.0 98-99 26.493087140027228 33.0 25.0 35.0 2.0 35.0 100-101 24.274265283838602 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42253093368178796 1101 2 0.4263222908320152 1101 3 0.4532946494201262 1101 4 0.4308643715111842 1101 5 0.42207461859518247 1101 6 0.40283409483157584 1101 7 0.33816499371687314 1101 8 0.42647852635482053 1101 9 0.5237272649318641 1101 10-11 0.5278056367931683 1101 12-13 0.5572949863896568 1101 14-15 0.749814024893297 1101 16-17 0.742684155160191 1101 18-19 0.819259038911845 1101 20-21 0.8000328538858312 1101 22-23 0.8306663643248768 1101 24-25 0.8649532945219605 1101 26-27 0.8493193673377135 1101 28-29 0.7752407763639084 1101 30-31 0.5988307039236744 1101 32-33 0.5198711981264097 1101 34-35 0.6312832073800934 1101 36-37 0.743399743209217 1101 38-39 0.683884463249619 1101 40-41 0.6914469119430038 1101 42-43 0.7138093199461579 1101 44-45 0.7451463214887397 1101 46-47 0.625644058533787 1101 48-49 0.8186660158408259 1101 50-51 0.7859803721360876 1101 52-53 0.7292756811857402 1101 54-55 0.8014829691865231 1101 56-57 0.7658737524350911 1101 58-59 0.7117589991182669 1101 60-61 0.8262664140114708 1101 62-63 0.6439304927302487 1101 64-65 0.4562700039469689 1101 66-67 0.6598564994824017 1101 68-69 0.712236273876286 1101 70-71 0.6656359333437365 1101 72-73 0.626903421434239 1101 74-75 0.6207913301039234 1101 76-77 0.7137109524025504 1101 78-79 0.7338557591758281 1101 80-81 0.9141426068949023 1101 82-83 0.8304195429969816 1101 84-85 0.7128087844134043 1101 86-87 0.7311875675048327 1101 88-89 0.85631031925735 1101 90-91 0.830728670127101 1101 92-93 1.0297143684706391 1101 94-95 1.0785554099857855 1101 96-97 1.0908352626410966 1101 98-99 1.074379086052577 1101 100-101 1.22262642457585 1102 1 0.15956962187155455 1102 2 0.1314427956657127 1102 3 0.2132551743677098 1102 4 0.2657921881147587 1102 5 0.321169571344619 1102 6 0.20150279151076944 1102 7 0.1506037868216623 1102 8 0.06753102887294204 1102 9 0.2577865062224447 1102 10-11 0.3288982623225962 1102 12-13 0.18996641225617594 1102 14-15 0.10236826332043591 1102 16-17 0.344994536004819 1102 18-19 0.18239217622791415 1102 20-21 0.13739973131373517 1102 22-23 0.10808336738696056 1102 24-25 0.04684398812509727 1102 26-27 0.13242298566431998 1102 28-29 -0.026059719809637727 1102 30-31 0.11656595956090854 1102 32-33 0.09755564913063353 1102 34-35 0.0333003771267002 1102 36-37 0.12585961243791388 1102 38-39 0.23238971520863316 1102 40-41 0.0384202649246248 1102 42-43 0.24837821776420554 1102 44-45 0.28629500904219185 1102 46-47 0.2831490908851677 1102 48-49 0.3703982582752303 1102 50-51 0.2837584939399349 1102 52-53 0.3657901434729851 1102 54-55 0.05170684480324894 1102 56-57 0.32297571167653416 1102 58-59 0.06003990986134511 1102 60-61 0.10966549383223878 1102 62-63 0.07801939657004908 1102 64-65 0.4286748121899322 1102 66-67 0.14786208201684303 1102 68-69 0.20436619234985898 1102 70-71 0.10312933735503549 1102 72-73 0.2395412775982244 1102 74-75 0.5008439544538241 1102 76-77 0.2724384348611011 1102 78-79 0.2019141988597788 1102 80-81 0.3415063182482321 1102 82-83 0.424570394810722 1102 84-85 0.2705214546093089 1102 86-87 0.4065425092971715 1102 88-89 0.40455987965410145 1102 90-91 0.20693243806625006 1102 92-93 0.2111252038952891 1102 94-95 0.20793669848502105 1102 96-97 0.07050235145104011 1102 98-99 0.16959567504177642 1102 100-101 0.12760645425003858 1103 1 0.17994721273066006 1103 2 0.240703035836912 1103 3 0.19582221196561633 1103 4 0.18561649579647366 1103 5 0.10006381821060728 1103 6 0.14761061432827915 1103 7 0.19175403998241336 1103 8 0.19397898310985795 1103 9 0.1412581969816884 1103 10-11 0.22231121070212367 1103 12-13 0.14014903892995534 1103 14-15 0.33964126666197103 1103 16-17 0.35208072934769064 1103 18-19 0.44177590140911605 1103 20-21 0.30133853681696365 1103 22-23 0.38702431949997873 1103 24-25 0.34099555709170914 1103 26-27 0.3420848543284336 1103 28-29 0.35607555983207106 1103 30-31 0.2897127503060162 1103 32-33 0.2795993315408083 1103 34-35 0.33893640002289516 1103 36-37 0.1890612443009303 1103 38-39 0.3577218719360573 1103 40-41 0.32350396439046847 1103 42-43 0.35056233827326366 1103 44-45 0.2787750979543233 1103 46-47 0.18101662088459136 1103 48-49 0.1802274965657702 1103 50-51 0.21991150545763816 1103 52-53 0.2911012187339992 1103 54-55 0.34546810443094245 1103 56-57 0.349907880272589 1103 58-59 0.3625774517220677 1103 60-61 0.4144001054607145 1103 62-63 0.4311476041215698 1103 64-65 0.35675450123189734 1103 66-67 0.36457635140391886 1103 68-69 0.3650827937024488 1103 70-71 0.38358427210321366 1103 72-73 0.23579176205069174 1103 74-75 0.1993387817103489 1103 76-77 0.2810896202952158 1103 78-79 0.30947885540587805 1103 80-81 0.25653198232357965 1103 82-83 0.26441975115746885 1103 84-85 0.14207259264217953 1103 86-87 0.16756009391425764 1103 88-89 0.14819157343179157 1103 90-91 0.17699840419084367 1103 92-93 0.1941937276358381 1103 94-95 0.3682601515522883 1103 96-97 0.46851439297951103 1103 98-99 0.4583086469387716 1103 100-101 0.5775451146922705 1104 1 0.15974502966235704 1104 2 0.05248011671976016 1104 3 0.13903432192646648 1104 4 0.20866002425286467 1104 5 0.23842556202843923 1104 6 0.2097267396225604 1104 7 0.14724666634549521 1104 8 0.0812492105920839 1104 9 0.24552513236584872 1104 10-11 0.36103827799112764 1104 12-13 0.23411498376281514 1104 14-15 0.12711382508788915 1104 16-17 0.4229894717049376 1104 18-19 0.35588756051037507 1104 20-21 0.35238935178850994 1104 22-23 0.12642299047439565 1104 24-25 0.1852138699796413 1104 26-27 0.12550905679015045 1104 28-29 0.174207140064091 1104 30-31 0.40127537624507426 1104 32-33 0.13295977298625417 1104 34-35 0.25952108148683095 1104 36-37 0.13305179346023266 1104 38-39 0.3164527911116366 1104 40-41 0.25554150549714905 1104 42-43 0.3182547731466272 1104 44-45 0.35617654969867374 1104 46-47 0.46557545515619125 1104 48-49 0.431064150562662 1104 50-51 0.42419190997343037 1104 52-53 0.47239515153157896 1104 54-55 0.6210129094260637 1104 56-57 0.5134050102429413 1104 58-59 0.4787340136300102 1104 60-61 0.481960405658441 1104 62-63 0.4197833360886989 1104 64-65 0.3754578425386228 1104 66-67 0.18919485072172648 1104 68-69 0.16218938905161195 1104 70-71 0.23284847624679372 1104 72-73 0.2543627281615706 1104 74-75 0.4531697786719562 1104 76-77 0.479592773960551 1104 78-79 0.5318871343608578 1104 80-81 0.37021452664967214 1104 82-83 0.2798386108636386 1104 84-85 0.34759189893201636 1104 86-87 0.5469996515423681 1104 88-89 0.38695807712671737 1104 90-91 0.4380442501930588 1104 92-93 0.44759780122860704 1104 94-95 0.659446876475922 1104 96-97 0.5691386943573917 1104 98-99 0.4899673419937116 1104 100-101 0.5204388026224649 1105 1 0.16990814611330052 1105 2 0.24901412202072137 1105 3 0.1878669979188352 1105 4 0.1555842540276231 1105 5 0.1683795471245233 1105 6 0.2124113347446226 1105 7 0.21483152845226527 1105 8 0.2455828225101726 1105 9 0.23712352326126762 1105 10-11 0.3223772030389753 1105 12-13 0.27790332710989674 1105 14-15 0.4513325476712282 1105 16-17 0.37573697272686246 1105 18-19 0.2857930368552175 1105 20-21 0.32808460168898534 1105 22-23 0.2579232853027662 1105 24-25 0.23084074830556034 1105 26-27 0.3495671585467619 1105 28-29 0.20451857957372255 1105 30-31 0.2883505126581696 1105 32-33 0.3233621744004438 1105 34-35 -0.056259872538149125 1105 36-37 0.10890545197515422 1105 38-39 0.3822020500871588 1105 40-41 0.2059815536114371 1105 42-43 0.3784613742281593 1105 44-45 0.2952501800305356 1105 46-47 0.41645759035149865 1105 48-49 0.531987362719768 1105 50-51 0.2956419422726171 1105 52-53 0.5059467712840942 1105 54-55 0.33425227141693625 1105 56-57 0.44442414445768463 1105 58-59 0.38386271241119374 1105 60-61 0.6041472423556797 1105 62-63 0.48074959665301265 1105 64-65 0.5285686083629813 1105 66-67 0.46520369281872576 1105 68-69 0.44558040130397814 1105 70-71 0.36757531378450636 1105 72-73 0.5153182652711656 1105 74-75 0.6434998611785936 1105 76-77 0.6229312030819365 1105 78-79 0.621448770726289 1105 80-81 0.34756284995712505 1105 82-83 0.33602240607994815 1105 84-85 0.43332489770303795 1105 86-87 0.6283576229383705 1105 88-89 0.6792456476654145 1105 90-91 0.5997071710542308 1105 92-93 0.5771258954805809 1105 94-95 0.525569164194831 1105 96-97 0.5571809317977774 1105 98-99 0.7029331092968754 1105 100-101 0.7652181815345394 1106 1 0.30691400640774447 1106 2 0.2928226409016297 1106 3 0.2893226469414749 1106 4 0.27372114073080667 1106 5 0.15368912354846742 1106 6 0.18087145168529872 1106 7 0.1473931505802497 1106 8 0.29476061003353493 1106 9 0.3437510455227155 1106 10-11 0.39289031742506353 1106 12-13 0.47073192557411403 1106 14-15 0.3145288187545958 1106 16-17 0.46850078306435705 1106 18-19 0.4295986667918754 1106 20-21 0.435339687888451 1106 22-23 0.1400283121604744 1106 24-25 0.12449601106214203 1106 26-27 0.06630750118502249 1106 28-29 -3.959881881243632E-4 1106 30-31 0.2639828836099767 1106 32-33 0.2864958832649478 1106 34-35 0.43188295307664504 1106 36-37 0.27388638020714495 1106 38-39 0.1336443436626169 1106 40-41 0.30129322333596065 1106 42-43 0.3901183589849566 1106 44-45 0.33649658424214124 1106 46-47 0.5635398987410909 1106 48-49 0.5180432339543231 1106 50-51 0.4899089981732132 1106 52-53 0.48230720354593615 1106 54-55 0.6701026487319908 1106 56-57 0.5831707120603937 1106 58-59 0.6631605852957492 1106 60-61 0.7064306449447457 1106 62-63 0.7249189967661067 1106 64-65 0.47488435197249856 1106 66-67 0.4537723298716614 1106 68-69 0.666367397076641 1106 70-71 0.6976269643188644 1106 72-73 0.655147902808114 1106 74-75 0.8106683832420707 1106 76-77 0.7039895796468194 1106 78-79 0.6974540522846873 1106 80-81 0.7360128034972178 1106 82-83 0.7256307315436672 1106 84-85 0.8201094459242526 1106 86-87 1.0199493270271276 1106 88-89 1.0504662692336098 1106 90-91 1.0229366034287786 1106 92-93 1.0721856587387002 1106 94-95 1.218041049084139 1106 96-97 1.2992242583839264 1106 98-99 1.1317667619927327 1106 100-101 1.2834388476208929 1107 1 0.3120327478294129 1107 2 0.35668035041740254 1107 3 0.31123777636747363 1107 4 0.29398189058061064 1107 5 0.3253864196895364 1107 6 0.3368828639475012 1107 7 0.4160038722768604 1107 8 0.4150364587969406 1107 9 0.42032550046766204 1107 10-11 0.4815037967704825 1107 12-13 0.581273903175294 1107 14-15 0.4133240512726246 1107 16-17 0.6488854069179126 1107 18-19 0.7124528542763429 1107 20-21 0.6932909435623387 1107 22-23 0.8020999460202134 1107 24-25 0.6144899924674121 1107 26-27 0.641144532528898 1107 28-29 0.6255186232612573 1107 30-31 0.6944664368037579 1107 32-33 0.6890721878268096 1107 34-35 0.5408675406508721 1107 36-37 0.5047765031060081 1107 38-39 0.6831891743293497 1107 40-41 0.7617594442351674 1107 42-43 0.7785865112868535 1107 44-45 0.8004965276212133 1107 46-47 0.8174664026820082 1107 48-49 0.8461987234055286 1107 50-51 0.8114920039265243 1107 52-53 0.8527402278059029 1107 54-55 0.8825146896961868 1107 56-57 0.9453874108297313 1107 58-59 0.9535750295036891 1107 60-61 1.0302547384070238 1107 62-63 0.94894346539445 1107 64-65 0.9880790199589242 1107 66-67 0.8842175590867996 1107 68-69 0.8263436896852028 1107 70-71 0.7953894940579502 1107 72-73 0.7951143077253313 1107 74-75 0.8837738627846186 1107 76-77 1.1002729401197477 1107 78-79 1.0122092559050735 1107 80-81 0.9453174565839468 1107 82-83 0.9867880423013311 1107 84-85 0.874052831289422 1107 86-87 0.8794220079545774 1107 88-89 0.8860105798266495 1107 90-91 0.9780032326277741 1107 92-93 1.081705156783709 1107 94-95 1.1003738522079338 1107 96-97 1.0136262103379288 1107 98-99 1.0525359467877102 1107 100-101 0.8864742010979931 1108 1 -0.14791143476232804 1108 2 -0.15576575986591834 1108 3 -0.028445958086244616 1108 4 -0.09982218842967683 1108 5 -0.1441556561202546 1108 6 -0.15239936946380084 1108 7 -0.13843865619909934 1108 8 -0.17749606370603033 1108 9 -0.2886584608404732 1108 10-11 -0.3352558740845595 1108 12-13 -0.2658710527770154 1108 14-15 -0.16371549110605343 1108 16-17 -0.13785902611236622 1108 18-19 -0.05216654517759878 1108 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-1.628394817545967 2316 56-57 -1.7947833410611906 2316 58-59 -1.7697520856230753 2316 60-61 -1.9664340962385296 2316 62-63 -1.948551118652592 2316 64-65 -2.462920884258125 2316 66-67 -2.2989540345610635 2316 68-69 -2.305245489186106 2316 70-71 -1.8611660323220818 2316 72-73 -2.132839651108025 2316 74-75 -2.616022977221089 2316 76-77 -2.7195410090667984 2316 78-79 -2.8808579027021466 2316 80-81 -2.6654151486320465 2316 82-83 -3.3164827940814874 2316 84-85 -3.2088790019449434 2316 86-87 -3.5940602768759966 2316 88-89 -3.278264894585533 2316 90-91 -3.584052198430438 2316 92-93 -3.7396198442670077 2316 94-95 -3.9155696619355957 2316 96-97 -4.321131065510528 2316 98-99 -4.507358241374018 2316 100-101 -4.772941309442942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6315.0 3 2465.0 4 2586.0 5 2805.0 6 3220.0 7 3909.0 8 4817.0 9 5771.0 10 7382.0 11 9112.0 12 11366.0 13 13584.0 14 15079.0 15 18069.0 16 22793.0 17 28283.0 18 25536.0 19 17356.0 20 17890.0 21 18790.0 22 20702.0 23 23232.0 24 26349.0 25 30259.0 26 35712.0 27 41519.0 28 49418.0 29 59446.0 30 72237.0 31 88840.0 32 110558.0 33 142509.0 34 190183.0 35 262976.0 36 386764.0 37 550441.0 38 461896.0 39 73602.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72403038926951 19.004331892629427 14.635725940460143 22.635911777640924 2 15.108541888611398 20.489075953125273 40.40800572835712 23.994376429906218 3 18.8316575850154 25.56027577111375 29.8608954842288 25.74717115964205 4 12.688987673091676 16.894998385945005 37.66278548161026 32.75322845935306 5 13.897055302514213 37.51632748718705 33.613319664323996 14.973297545974743 6 31.063961147018766 36.2384387769086 18.035123464213697 14.66247661185894 7 27.863730684180165 33.26609824894125 21.030209667946266 17.83996139893232 8 26.435375595189832 34.12248119018395 20.144327418015486 19.297815796610738 9 26.854154974710433 16.64872832285105 20.397888773415655 36.09922792902287 10-11 24.643957601124534 26.857798826369212 27.40593000673567 21.09231356577059 12-13 25.892910962859244 24.372113079487296 27.83771935878456 21.897256598868896 14-15 23.046262902515416 25.168162267393946 26.426891846254936 25.3586829838357 16-17 22.119493984528578 28.670524128878576 26.710506387557036 22.49947549903581 18-19 22.092770947940426 27.10139792700926 29.33406989355517 21.471761231495147 20-21 23.113346316906807 26.08791584296445 29.242843835842347 21.555894004286397 22-23 22.788951626382538 26.128240063392248 29.122710008224434 21.960098302000777 24-25 22.46966510612411 26.34164637123219 29.205991617351145 21.98269690529255 26-27 22.293795893168177 26.56303907231024 29.033884644985747 22.109280389535833 28-29 22.085083387097196 26.50500549569991 29.263384146368082 22.146526970834817 30-31 22.297115026512508 26.257849034195363 29.161452601462784 22.283583337829345 32-33 22.123815156050107 26.393766900619163 29.114418468540904 22.367999474789823 34-35 22.46436227850939 26.45230197279028 28.883622030800993 22.199713717899336 36-37 22.520226559112956 26.24372582499061 28.87141064459067 22.36463697130576 38-39 22.334169665414787 26.438691969640622 28.98094943770199 22.246188927242603 40-41 22.535298324621664 26.29671056491288 28.92793386767389 22.240057242791572 42-43 22.308005110190894 26.413495597200743 28.702937242323234 22.575562050285132 44-45 22.458058655289438 26.54471971272446 28.49422161260579 22.50300001938031 46-47 22.601034063908173 26.72960746042906 28.131790158322158 22.537568317340607 48-49 22.679204612494683 26.877440060563174 27.779567366491882 22.663787960450264 50-51 22.526186550659517 26.932395722154816 27.814601333958855 22.72681639322681 52-53 22.838842353991264 27.056805517069904 27.499444322512595 22.604907806426233 54-55 22.60749855856051 26.978892995424765 27.514259861419255 22.899348584595472 56-57 22.577158084893362 27.32534120882018 27.439796282191907 22.65770442409455 58-59 22.81727255013022 26.911307349172837 27.494585566212535 22.776834534484408 60-61 22.777979825950677 26.788939663798438 27.898576977531214 22.53450353271967 62-63 22.617274094933975 26.88790269972775 27.8811460467923 22.613677158545972 64-65 22.547303757412546 26.98459978363852 27.72601149690997 22.742084962038966 66-67 22.52192641233548 27.257608866298437 27.418358025486906 22.802106695879182 68-69 22.438524053253477 26.96174725175295 27.741233854365177 22.858494840628396 70-71 22.441252942135257 27.038469755993848 27.664972269288818 22.85530503258208 72-73 22.65835311848849 26.94788766478366 27.42159648480154 22.97216273192631 74-75 22.47705957438635 27.304737907274408 27.313486263683494 22.90471625465575 76-77 22.626691478274473 27.09002171015683 27.35890144371146 22.924385367857234 78-79 22.58715750461178 27.260443803014933 27.25148482413594 22.900913868237346 80-81 22.508996847172067 27.347776980232446 27.177362821232347 22.96586335136314 82-83 22.488578254673484 27.271792131220835 27.290007544639117 22.949622069466567 84-85 22.656059698333852 27.161425315880578 27.15690282065757 23.025612165128003 86-87 22.59371177355343 27.016051003292496 27.362852853461245 23.027384369692832 88-89 22.671582392056116 26.994857598322515 27.14169352515749 23.191866484463883 90-91 22.464217704755846 27.081514568112908 27.435398682069884 23.01886904506136 92-93 22.742403236869908 26.926917677562606 27.294181679524517 23.036497406042965 94-95 22.599614486224056 26.886015006714796 27.262169448303908 23.252201058757237 96-97 22.533754522721875 26.838006230529594 27.311791905899447 23.316447340849084 98-99 22.369693543183917 26.810111490763887 27.26040262290524 23.55979234314696 100-101 22.378137402531415 26.898341920132456 27.31232896148014 23.411191715855985 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 504.0 1 379.5 2 313.0 3 400.0 4 377.5 5 383.5 6 649.0 7 1358.0 8 2403.5 9 3035.0 10 2868.5 11 2674.0 12 2839.0 13 3053.5 14 3297.0 15 3526.0 16 3781.0 17 4024.5 18 4201.0 19 4443.0 20 4829.5 21 5508.0 22 6573.0 23 8013.0 24 9668.0 25 12190.5 26 15898.5 27 19669.5 28 22359.5 29 25688.0 30 31186.5 31 36941.5 32 42022.0 33 47639.5 34 55005.0 35 60586.0 36 65996.5 37 73127.0 38 77508.5 39 78866.0 40 80148.0 41 84616.0 42 88889.5 43 93602.5 44 99504.5 45 101444.5 46 100633.5 47 102594.5 48 109802.0 49 112804.0 50 109529.5 51 106404.0 52 102760.0 53 101321.5 54 100048.0 55 94169.5 56 86128.5 57 77954.0 58 71952.0 59 65754.5 60 56513.0 61 45474.5 62 34745.0 63 26509.0 64 19992.0 65 14545.0 66 10242.5 67 7591.5 68 5411.5 69 3292.0 70 2013.5 71 1256.0 72 842.0 73 586.0 74 390.0 75 270.0 76 177.5 77 90.5 78 56.5 79 39.0 80 27.5 81 24.5 82 23.5 83 14.0 84 7.5 85 7.0 86 9.0 87 12.0 88 10.5 89 4.5 90 3.0 91 3.0 92 3.0 93 3.5 94 1.5 95 1.5 96 2.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03743287311617844 2 0.02943648510908435 3 0.024233595095734924 4 0.05017820771263845 5 0.09847080427949925 6 0.11764118426157198 7 0.05922215746738678 8 0.08803010552787859 9 0.07469115260774783 10-11 0.07544190519356671 12-13 0.07233413867552578 14-15 0.06632811798897476 16-17 0.05407164554142007 18-19 0.06428537258104898 20-21 0.059885613240901144 22-23 0.0343076472356766 24-25 0.009043949754748336 26-27 0.002688741818979235 28-29 0.00665201709760447 30-31 0.005045755751201292 32-33 0.006198073673620964 34-35 0.006425045385612718 36-37 0.005918723874246497 38-39 0.0034220350423372083 40-41 0.007018663709283458 42-43 0.0036140880294071535 44-45 0.002147501582691207 46-47 0.0015189645340986586 48-49 2.2697171199175358E-4 50-51 0.002147501582691207 52-53 0.005988561324090114 54-55 0.01173269157372757 56-57 0.007821794382485047 58-59 0.005272727463193045 60-61 0.002252257757456632 62-63 0.008555087605843021 64-65 0.0023395545697611525 66-67 0.022225768412730947 68-69 0.04651174159584858 70-71 0.059833235153518426 72-73 0.00398073464108614 74-75 0.013897652518879682 76-77 0.021544853276755687 78-79 0.02596207197936443 80-81 0.07804335020024143 82-83 0.029087297859866267 84-85 0.011505719861735816 86-87 0.03209030820314178 88-89 0.04939253640189776 90-91 0.044486455550383705 92-93 0.04450391491284461 94-95 0.011924744560797515 96-97 0.0165165568880153 98-99 0.038043950802310086 100-101 0.07786875657563239 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2863793.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.390723481961125 #Duplication Level Percentage of deduplicated Percentage of total 1 70.99660439738672 32.93583842757352 2 14.343107329557556 13.307742519951887 3 5.6908040771097586 7.920015549936473 4 2.7058985260055173 5.021143611606725 5 1.5966695609839432 3.703532804783518 6 1.0242615187614328 2.850973973404509 7 0.6470187496371604 2.101096753144322 8 0.488740048738966 1.813840356448766 9 0.3548213866010098 1.4814378748164125 >10 1.931887005233161 16.335068966408247 >50 0.1427707562749332 4.6013792541987275 >100 0.07470077657667146 6.1100191735048455 >500 0.0020369003498360463 0.6102866044964562 >1k 4.5264452216970096E-4 0.27907015621723097 >5k 1.5088150738990034E-4 0.4912250705624597 >10k+ 7.544075369495017E-5 0.4373289029458889 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12496 0.43634438662291586 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8429 0.2943299323659217 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5607 0.19578929063657882 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 3.4918724921808244E-5 0.0 0.0 3.4918724921808244E-5 7 0.0 3.4918724921808244E-5 0.0 0.0 6.983744984361649E-5 8 0.0 3.4918724921808244E-5 0.0 0.0 6.983744984361649E-5 9 0.0 3.4918724921808244E-5 0.0 0.0 6.983744984361649E-5 10-11 0.0 3.4918724921808244E-5 0.0 0.0 6.983744984361649E-5 12-13 0.0 5.2378087382712366E-5 0.0 1.7459362460904122E-5 8.72968123045206E-5 14-15 0.0 6.983744984361649E-5 0.0 3.4918724921808244E-5 1.745936246090412E-4 16-17 0.0 6.983744984361649E-5 0.0 3.4918724921808244E-5 2.0951234953084946E-4 18-19 0.0 8.72968123045206E-5 0.0 3.4918724921808244E-5 2.444310744526577E-4 20-21 0.0 1.0475617476542473E-4 0.0 6.983744984361649E-5 2.444310744526577E-4 22-23 0.0 1.0475617476542473E-4 0.0 1.571342621481371E-4 3.317278867571783E-4 24-25 0.0 1.7459362460904123E-4 0.0 4.888621489053154E-4 3.841059741398907E-4 26-27 0.0 1.7459362460904123E-4 0.0 7.507525858188773E-4 3.841059741398907E-4 28-29 0.0 1.7459362460904123E-4 0.0 0.0017808549710122204 3.841059741398907E-4 30-31 0.0 2.0951234953084946E-4 0.0 0.004399759340147839 3.841059741398907E-4 32-33 0.0 2.0951234953084946E-4 0.0 0.008083684819398609 3.841059741398907E-4 34-35 1.7459362460904122E-5 2.0951234953084946E-4 0.0 0.011016857712830501 5.237808738271237E-4 36-37 3.4918724921808244E-5 2.0951234953084946E-4 0.0 0.013059603120756283 5.237808738271237E-4 38-39 3.4918724921808244E-5 2.0951234953084946E-4 0.0 0.015189645340986588 5.237808738271237E-4 40-41 3.4918724921808244E-5 2.444310744526577E-4 0.0 0.01749428118582593 5.237808738271237E-4 42-43 3.4918724921808244E-5 2.444310744526577E-4 0.0 0.020427454079257824 5.237808738271237E-4 44-45 3.4918724921808244E-5 2.444310744526577E-4 0.0 0.02350030187237695 5.237808738271237E-4 46-47 6.983744984361649E-5 2.444310744526577E-4 0.0 0.02828416718666468 5.237808738271237E-4 48-49 6.983744984361649E-5 2.444310744526577E-4 0.0 0.03383624444923219 5.586995987489319E-4 50-51 6.983744984361649E-5 2.7934979937446595E-4 0.0 0.041413607757264576 5.586995987489319E-4 52-53 8.72968123045206E-5 3.4918724921808247E-4 0.0 0.06018242240273651 5.936183236707401E-4 54-55 1.0475617476542473E-4 3.4918724921808247E-4 0.0 0.08120349480566508 6.285370485925484E-4 56-57 1.0475617476542473E-4 3.4918724921808247E-4 0.0 0.10601324886260984 6.285370485925484E-4 58-59 1.0475617476542473E-4 3.4918724921808247E-4 0.0 0.14416195583968533 6.285370485925484E-4 60-61 1.0475617476542473E-4 3.4918724921808247E-4 0.0 0.19957797229059504 6.285370485925484E-4 62-63 1.0475617476542473E-4 3.4918724921808247E-4 0.0 0.2334142167398272 6.285370485925484E-4 64-65 1.0475617476542473E-4 3.4918724921808247E-4 0.0 0.2685773727360881 6.285370485925484E-4 66-67 1.0475617476542473E-4 3.666466116789866E-4 0.0 0.3382926070424783 6.285370485925484E-4 68-69 1.0475617476542473E-4 4.015653366007948E-4 0.0 0.43049550019851296 6.285370485925484E-4 70-71 1.2221553722632886E-4 4.1902469906169893E-4 0.0 0.5028470982364996 6.285370485925484E-4 72-73 1.3967489968723298E-4 4.1902469906169893E-4 0.0 0.5656309656459109 7.332932233579732E-4 74-75 1.3967489968723298E-4 4.1902469906169893E-4 0.0 0.6888242271700504 7.332932233579732E-4 76-77 1.3967489968723298E-4 4.1902469906169893E-4 0.0 0.7797002087790563 7.507525858188773E-4 78-79 1.3967489968723298E-4 4.539434239835072E-4 0.0 0.8459235705932657 7.682119482797814E-4 80-81 1.3967489968723298E-4 4.539434239835072E-4 0.0 0.9310205032277123 8.031306732015896E-4 82-83 1.3967489968723298E-4 4.539434239835072E-4 0.0 1.095138510360211 8.380493981233979E-4 84-85 1.3967489968723298E-4 4.539434239835072E-4 0.0 1.2680734955354664 8.380493981233979E-4 86-87 1.3967489968723298E-4 4.888621489053154E-4 0.0 1.4813570673578713 8.380493981233979E-4 88-89 1.3967489968723298E-4 5.237808738271237E-4 0.0 1.7626797746904193 8.380493981233979E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7830 0.0 42.06523 1 GTATCAA 13895 0.0 40.533264 1 TATCAAC 17450 0.0 32.167244 2 ATCAACG 17640 0.0 31.82077 3 TCAACGC 17745 0.0 31.659819 4 CAACGCA 17890 0.0 31.377195 5 AACGCAG 18485 0.0 30.338854 6 ACGCAGA 20685 0.0 26.785278 7 CGCAGAG 20920 0.0 26.143682 8 GTACATG 14655 0.0 24.712744 1 GCAGAGT 21740 0.0 24.305153 9 GAGTACT 9935 0.0 23.868633 12-13 TACATGG 14895 0.0 23.772516 2 ACATGGG 15005 0.0 22.90138 3 AGTACTT 10675 0.0 21.72435 12-13 AGAGTAC 18250 0.0 21.480303 10-11 GTACTTT 11200 0.0 20.854162 14-15 CATGGGA 8815 0.0 20.219757 4 CAGAGTA 20825 0.0 19.748377 10-11 CATGGGG 7100 0.0 19.413626 4 >>END_MODULE