FastQCFastQC Report
Thu 26 May 2016
SRR937136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863793
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129890.4535593180093673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97790.34147021101036285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63480.22166406580363873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81150.042.327331
GTATCAA138600.039.9674531
TATCAAC179300.030.8361662
ATCAACG180750.030.5609173
TCAACGC183600.030.1382624
CAACGCA185950.029.7318365
AACGCAG192050.028.8152286
ACGCAGA218250.025.2254927
CGCAGAG220850.024.8652958
GCAGAGT233000.022.9570279
GTACATG141000.021.968461
GAGTACT116800.021.5344412-13
TGTACCG3101.0913936E-1121.45025
AGAGTAC199050.021.33578310-11
AGTACTT123150.021.27264812-13
TACATGG146350.020.7069282
ACATGGG144900.020.1263453
GTACTTT129050.019.54482714-15
CAGAGTA226500.018.634710-11
TACTTTT148550.017.52278714-15
Produced by FastQC (version 0.11.5)