FastQCFastQC Report
Thu 26 May 2016
SRR937135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377151
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57830.41992490293366525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42350.30751892857065055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.18756113164061167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.048.131811
GTATCAA59800.041.2607041
TATCAAC76600.032.2734262
ATCAACG76050.032.1270753
TCAACGC76500.031.9380914
CAACGCA76950.031.7466985
AACGCAG79450.030.747756
ACGCAGA91100.026.657237
CGCAGAG90950.026.3886388
GCAGAGT94400.024.8200849
GTACATG60250.024.5399591
TACATGG60950.023.8681182
CGCGGAA701.778717E-523.75333482-83
CTAGGCG1050.0021872422.635394
ACATGGG62550.022.4942593
GAGTACT51650.022.08276412-13
AGAGTAC83750.021.3637210-11
GTACTTT53350.021.11070614-15
GTGTAGC9350.020.8469581
GTATAGA3700.020.5583721
Produced by FastQC (version 0.11.5)