FastQCFastQC Report
Thu 26 May 2016
SRR937135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377151
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62860.4564495832337921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51120.371201124640653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34010.24695912067739847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42900.040.7456051
GTATCAA69500.035.3342631
TATCAAC86800.028.2360882
ATCAACG86750.028.1976093
TCAACGC87200.028.0520944
CAACGCA88900.027.622525
AACGCAG91900.026.7208066
TTACGTG950.001226992624.9989015
ACGCAGA105800.022.9857587
CGCAGAG106700.022.7473628
GAGTACT59150.022.32321212-13
GTACTTT61250.021.32636814-15
GCAGAGT112250.021.1148859
GTACATG60500.020.7271141
TACATGG61250.020.0848312
AGAGTAC95900.019.78666910-11
AGTACTT61300.019.44822112-13
ACATGGG62350.019.273413
TAGCGGT1507.7244017E-418.9991654
ACTTTTT71150.018.1920616-17
Produced by FastQC (version 0.11.5)