##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937135_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1377151 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.977676376809804 31.0 31.0 34.0 30.0 34.0 2 31.98073196040231 31.0 31.0 34.0 30.0 34.0 3 32.163836064454806 33.0 31.0 34.0 30.0 34.0 4 35.93358825575409 37.0 35.0 37.0 35.0 37.0 5 35.844515960849606 37.0 35.0 37.0 35.0 37.0 6 35.87871918184716 37.0 35.0 37.0 35.0 37.0 7 35.8116829599659 37.0 35.0 37.0 35.0 37.0 8 35.7944938499845 37.0 35.0 37.0 33.0 37.0 9 37.45354576222942 39.0 37.0 39.0 34.0 39.0 10-11 37.37226346275753 39.0 37.0 39.0 34.0 39.0 12-13 37.26996131869345 39.0 37.0 39.0 34.0 39.0 14-15 38.439023389591995 40.0 38.0 41.0 34.0 41.0 16-17 38.35840368993669 40.0 38.0 41.0 33.0 41.0 18-19 38.425870510931624 40.0 38.0 41.0 33.5 41.0 20-21 38.49968776118232 40.0 38.0 41.0 34.0 41.0 22-23 38.42650079766126 40.0 38.0 41.0 34.0 41.0 24-25 38.31584844363472 40.0 38.0 41.0 34.0 41.0 26-27 38.13811593645141 40.0 38.0 41.0 33.0 41.0 28-29 37.962473614004566 40.0 37.0 41.0 33.0 41.0 30-31 37.82336686390963 40.0 37.0 41.0 33.0 41.0 32-33 37.816614881011596 40.0 37.0 41.0 33.0 41.0 34-35 37.72381169530429 40.0 37.0 41.0 33.0 41.0 36-37 37.55472348348148 40.0 37.0 41.0 33.0 41.0 38-39 37.40630947514107 40.0 36.5 41.0 32.0 41.0 40-41 37.229776908995454 40.0 36.0 41.0 31.5 41.0 42-43 37.00620120814638 39.5 36.0 41.0 31.0 41.0 44-45 36.62254937911674 39.0 36.0 41.0 30.0 41.0 46-47 36.36132021833481 39.0 35.0 41.0 30.0 41.0 48-49 36.020628093796546 39.0 35.0 40.5 29.5 41.0 50-51 35.758431718816595 39.0 35.0 40.0 28.0 41.0 52-53 35.51580146258471 39.0 35.0 40.0 28.0 41.0 54-55 35.2813623923593 38.0 35.0 40.0 27.0 41.0 56-57 35.00304578074591 38.0 34.0 40.0 26.5 41.0 58-59 34.67545280074589 38.0 34.0 40.0 26.0 41.0 60-61 34.335893449592675 37.5 34.0 40.0 26.0 41.0 62-63 33.96135536335522 37.0 33.0 40.0 25.0 41.0 64-65 33.5738121672932 36.5 33.0 39.0 23.5 41.0 66-67 33.19585107224988 36.0 32.0 39.0 23.0 40.0 68-69 32.749705733067756 35.5 32.0 39.0 22.0 40.0 70-71 32.27149709799434 35.0 31.0 38.0 21.5 40.0 72-73 31.915236600779433 35.0 31.0 37.5 20.0 39.5 74-75 31.469666724999655 35.0 31.0 37.0 18.5 39.0 76-77 30.06117702416075 33.5 29.5 35.5 18.0 38.0 78-79 30.800650400718585 34.0 30.5 36.0 18.0 38.5 80-81 30.63441118657286 34.0 30.5 36.0 17.5 37.5 82-83 30.2881946133721 34.0 30.0 35.0 13.5 37.0 84-85 29.89094187928557 34.0 30.0 35.0 8.5 36.5 86-87 29.517280240147958 34.0 29.5 35.0 7.0 36.0 88-89 29.145163457021056 34.0 29.0 35.0 2.0 36.0 90-91 28.70318577991811 34.0 29.0 35.0 2.0 35.0 92-93 28.2220192266498 33.5 29.0 35.0 2.0 35.0 94-95 27.7086510484326 33.0 27.0 35.0 2.0 35.0 96-97 27.026770121794925 33.0 26.0 35.0 2.0 35.0 98-99 26.274395109904432 33.0 25.0 34.0 2.0 35.0 100-101 23.333980442231827 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3685818759521986 1101 2 0.27819817881274034 1101 3 0.306495790798607 1101 4 0.2837723772476579 1101 5 0.333207071434785 1101 6 0.2528559556076928 1101 7 0.2805063858859711 1101 8 0.395290186174428 1101 9 0.4542331642603372 1101 10-11 0.392101689697391 1101 12-13 0.4576761518163295 1101 14-15 0.623886897565761 1101 16-17 0.602392191579348 1101 18-19 0.620268024361259 1101 20-21 0.5445543215960384 1101 22-23 0.5705044573644358 1101 24-25 0.5206646584357202 1101 26-27 0.5173596223192831 1101 28-29 0.5851354217258873 1101 30-31 0.5433536790045963 1101 32-33 0.6782128597302872 1101 34-35 0.7210064025279408 1101 36-37 0.7076154743557836 1101 38-39 0.8391820842982369 1101 40-41 0.8153143040074937 1101 42-43 0.8748803178718774 1101 44-45 1.0636797728013363 1101 46-47 1.143325671761339 1101 48-49 1.062294831586165 1101 50-51 1.034107994006689 1101 52-53 0.969672266705011 1101 54-55 0.8919189496172137 1101 56-57 1.1161536997254302 1101 58-59 1.0059175310110575 1101 60-61 1.086077823021192 1101 62-63 1.1343899551721321 1101 64-65 1.0340001722068948 1101 66-67 1.1063660894298195 1101 68-69 0.8800121348235805 1101 70-71 0.8647581433464424 1101 72-73 1.0321613203554172 1101 74-75 1.001636533799143 1101 76-77 0.8195175159959867 1101 78-79 0.8794198312134718 1101 80-81 0.9979543644887556 1101 82-83 1.034885243538124 1101 84-85 1.1160218499507906 1101 86-87 0.9659603830310424 1101 88-89 0.9911486970996499 1101 90-91 1.1213866077482812 1101 92-93 1.1658726206615029 1101 94-95 1.0864705780462032 1101 96-97 1.2557293275834986 1101 98-99 1.18178121060755 1101 100-101 1.0391692661472582 1102 1 0.2933238151914601 1102 2 0.15934559423856953 1102 3 0.176234161738563 1102 4 0.22954228982512603 1102 5 0.1836832370335415 1102 6 0.2055912271418876 1102 7 0.2578358339169924 1102 8 0.32577865136069306 1102 9 0.3675141388881684 1102 10-11 0.41757101054667345 1102 12-13 0.3434267217283917 1102 14-15 0.5518588490884824 1102 16-17 0.524441298343568 1102 18-19 0.5241169503402503 1102 20-21 0.4682479419025469 1102 22-23 0.45332379757896035 1102 24-25 0.40652148723830095 1102 26-27 0.3826502766644282 1102 28-29 0.40859111241351087 1102 30-31 0.4684597847146108 1102 32-33 0.5380198136087699 1102 34-35 0.5450378906329121 1102 36-37 0.7020025379244075 1102 38-39 0.6052137936280886 1102 40-41 0.5807916611892594 1102 42-43 0.6353040892917576 1102 44-45 0.7208208700961123 1102 46-47 0.7607847975618682 1102 48-49 0.7858424144463285 1102 50-51 0.8474013681924504 1102 52-53 0.881214078369112 1102 54-55 0.8664035939982142 1102 56-57 0.8712448550191709 1102 58-59 0.8502930818561794 1102 60-61 0.8622354886407422 1102 62-63 0.9875800760299285 1102 64-65 0.7164876428486409 1102 66-67 0.8626256087913546 1102 68-69 0.8391680877239622 1102 70-71 0.7624332504196047 1102 72-73 0.8491888318947538 1102 74-75 0.7635808231819254 1102 76-77 0.7185032837543623 1102 78-79 0.9687251883062302 1102 80-81 0.8144498559836926 1102 82-83 1.030184940369736 1102 84-85 1.044958435483391 1102 86-87 0.7865438588222382 1102 88-89 0.8712086922559195 1102 90-91 1.0874422938566148 1102 92-93 0.9657665713863999 1102 94-95 0.9795599448367405 1102 96-97 1.1922724533750007 1102 98-99 1.246808668614328 1102 100-101 1.3408125381213232 1103 1 0.07682825733925469 1103 2 -0.18490543770816714 1103 3 -0.009983416981128812 1103 4 0.1478901286538985 1103 5 0.1599355147577981 1103 6 0.1294041901215408 1103 7 0.1654384881584079 1103 8 0.2251969644669316 1103 9 0.2019344343149001 1103 10-11 0.25479244944444446 1103 12-13 0.28595736815576345 1103 14-15 0.2474510450205898 1103 16-17 0.3622294175359073 1103 18-19 0.40418585343208946 1103 20-21 0.41336488467119636 1103 22-23 0.37195197170004946 1103 24-25 0.39667484266261965 1103 26-27 0.4271993725477685 1103 28-29 0.37100134899989 1103 30-31 0.43752418423589035 1103 32-33 0.6018895966281903 1103 34-35 0.5282157403975845 1103 36-37 0.5260011125626178 1103 38-39 0.6474353642092154 1103 40-41 0.5597036774451709 1103 42-43 0.5237278148488045 1103 44-45 0.7494518351971919 1103 46-47 0.6213539595131223 1103 48-49 0.5203990065590105 1103 50-51 0.42751274579295284 1103 52-53 0.6043037904350754 1103 54-55 0.5906794283713168 1103 56-57 0.6209503350438652 1103 58-59 0.7506757766489969 1103 60-61 0.6060522483430262 1103 62-63 0.6585440388043793 1103 64-65 0.2733291584904052 1103 66-67 0.5627119188312264 1103 68-69 0.5029879194485503 1103 70-71 0.5078990568493182 1103 72-73 0.5176076825165161 1103 74-75 0.5118261204513423 1103 76-77 0.47883702415716556 1103 78-79 0.6150588852270111 1103 80-81 0.7539465710585702 1103 82-83 0.6301281839621922 1103 84-85 0.6093376297969364 1103 86-87 0.4586184690174271 1103 88-89 0.8887415790678084 1103 90-91 1.1571631481333462 1103 92-93 1.1542105708766357 1103 94-95 1.0143415829104399 1103 96-97 1.216523753991119 1103 98-99 1.179995806915958 1103 100-101 1.2977149639407308 1104 1 0.22110760014226472 1104 2 0.16096603166071688 1104 3 0.1683430752838433 1104 4 0.16888083710477986 1104 5 0.1139420572327623 1104 6 0.18767405896122824 1104 7 0.18702895563391309 1104 8 0.12603844909988027 1104 9 0.07909486899657736 1104 10-11 0.2572730467684252 1104 12-13 0.2453443792787482 1104 14-15 0.30718419298165145 1104 16-17 0.370494645638324 1104 18-19 0.3935826797679809 1104 20-21 0.2829048643548475 1104 22-23 0.20558368407637317 1104 24-25 0.327861604579077 1104 26-27 0.28369684414978025 1104 28-29 0.387919140643433 1104 30-31 0.3891009309911766 1104 32-33 0.448228569368311 1104 34-35 0.5530561172554656 1104 36-37 0.4877899463699862 1104 38-39 0.3774478802669208 1104 40-41 0.46760422719570016 1104 42-43 0.4928774152845463 1104 44-45 0.5138678621816339 1104 46-47 0.5525462946013633 1104 48-49 0.7158562499353494 1104 50-51 0.6727584551473313 1104 52-53 0.6386717188035291 1104 54-55 0.6326674902086609 1104 56-57 0.6640252725546816 1104 58-59 0.6949370923848264 1104 60-61 0.702118939488777 1104 62-63 0.892067224377918 1104 64-65 0.7411507363968113 1104 66-67 0.8013656377337313 1104 68-69 0.7850552532238808 1104 70-71 0.589557705650968 1104 72-73 0.644828403912161 1104 74-75 0.7333826344495407 1104 76-77 0.6836851691590908 1104 78-79 0.690950908882126 1104 80-81 0.8694715627684779 1104 82-83 0.9977926841655069 1104 84-85 1.010474063828692 1104 86-87 0.8031815291345872 1104 88-89 1.1075601652527247 1104 90-91 1.0885316811909362 1104 92-93 1.2621270041667358 1104 94-95 0.9962617849507787 1104 96-97 1.1200208549772022 1104 98-99 1.3572988719246517 1104 100-101 1.2104183016490033 1105 1 0.16698783579991172 1105 2 -0.06071253934806364 1105 3 0.07232969122482302 1105 4 0.02861934644344899 1105 5 0.0456018607817299 1105 6 0.09253335525331607 1105 7 0.16002235894362116 1105 8 0.020851081415692363 1105 9 0.08799432791386153 1105 10-11 0.17644876739579018 1105 12-13 0.21272426165888447 1105 14-15 0.22869598565933558 1105 16-17 0.22042353302965267 1105 18-19 0.22811349980279516 1105 20-21 0.13825411280754452 1105 22-23 0.11539082459201211 1105 24-25 0.13051543048966607 1105 26-27 0.11733539358316136 1105 28-29 0.1567242790354939 1105 30-31 0.2138046284550228 1105 32-33 0.22014163567347111 1105 34-35 0.2878131393806882 1105 36-37 0.12383914140659869 1105 38-39 0.26505574092015394 1105 40-41 0.026562652766457973 1105 42-43 0.26770629444796157 1105 44-45 0.3295817243733694 1105 46-47 0.283051318194552 1105 48-49 0.4779769337134496 1105 50-51 0.47517981008740406 1105 52-53 0.49730377080388877 1105 54-55 0.4514708654781856 1105 56-57 0.4895008258812936 1105 58-59 0.42898181300825655 1105 60-61 0.40033021903250443 1105 62-63 0.45765786389510765 1105 64-65 0.41837811520826307 1105 66-67 0.5225207572538508 1105 68-69 0.4284462210736635 1105 70-71 0.31695884998278956 1105 72-73 0.44131430480900136 1105 74-75 0.4068387594719063 1105 76-77 0.32370687515645 1105 78-79 0.35209508398239464 1105 80-81 0.5077829093108654 1105 82-83 0.6929258129766289 1105 84-85 0.7871337171857427 1105 86-87 0.7166183512302986 1105 88-89 0.7782676475529335 1105 90-91 0.9400037579011347 1105 92-93 0.793153650825225 1105 94-95 0.6137180040063477 1105 96-97 0.9457435066612483 1105 98-99 1.0641390037278562 1105 100-101 0.996166525139806 1106 1 0.36880368319536316 1106 2 0.26530096780997425 1106 3 0.22663110025844446 1106 4 0.252816535901097 1106 5 0.29830517360976927 1106 6 0.30154863075795646 1106 7 0.2704447868458715 1106 8 0.23543194564805958 1106 9 0.39010270498555855 1106 10-11 0.3024982528821738 1106 12-13 0.3090072065203344 1106 14-15 0.4976285815545154 1106 16-17 0.6147577693484791 1106 18-19 0.492246868879306 1106 20-21 0.5244828785392386 1106 22-23 0.510161268748007 1106 24-25 0.5212346678431672 1106 26-27 0.5015331474067395 1106 28-29 0.3670615817425684 1106 30-31 0.39341831997260357 1106 32-33 0.4620102983143326 1106 34-35 0.49739231051525223 1106 36-37 0.40649876290517994 1106 38-39 0.7031920635117288 1106 40-41 0.6641266142390734 1106 42-43 0.5833991947244996 1106 44-45 0.6957082458098682 1106 46-47 0.5381844615246862 1106 48-49 0.483447285689671 1106 50-51 0.49182554818398216 1106 52-53 0.44818935501668733 1106 54-55 0.5504681753338971 1106 56-57 0.6038620577040135 1106 58-59 0.5744024537517234 1106 60-61 0.4190097908668875 1106 62-63 0.6813532379756637 1106 64-65 0.687145398915014 1106 66-67 0.8754651013665722 1106 68-69 0.8446324712382776 1106 70-71 0.8526460595694871 1106 72-73 0.894038781359594 1106 74-75 1.0181921560142229 1106 76-77 0.9132990267075165 1106 78-79 0.9050184995250312 1106 80-81 0.9569238973274956 1106 82-83 1.176982005011542 1106 84-85 1.02711833821402 1106 86-87 0.9629779557978999 1106 88-89 1.009757620651495 1106 90-91 1.2604382726404886 1106 92-93 1.308005645858806 1106 94-95 1.5811750181343065 1106 96-97 1.9254738338053663 1106 98-99 1.7919690637387653 1106 100-101 1.5671247244362299 1107 1 0.2332358376268182 1107 2 0.12713812905181854 1107 3 0.14103742217849913 1107 4 0.13130392493872023 1107 5 0.16541451558524756 1107 6 0.16614517389825068 1107 7 0.09383847431944758 1107 8 0.01267350311365334 1107 9 0.11616066374747192 1107 10-11 0.15308946513147959 1107 12-13 0.22507209309212328 1107 14-15 0.33109401295745755 1107 16-17 0.24310718929118025 1107 18-19 0.21747114204637086 1107 20-21 0.183291177820621 1107 22-23 0.1639058086944658 1107 24-25 0.09345415025357084 1107 26-27 0.10136406187763924 1107 28-29 0.10510817419946505 1107 30-31 0.20869237943434626 1107 32-33 0.24297702134283128 1107 34-35 0.28196090757504066 1107 36-37 0.17235346677066588 1107 38-39 0.17530175774872703 1107 40-41 0.17053190285868425 1107 42-43 0.08894731364798503 1107 44-45 0.19677677241735125 1107 46-47 0.4612123841210902 1107 48-49 0.41337072751954906 1107 50-51 0.6359738972516737 1107 52-53 0.6515205747809105 1107 54-55 0.5255574149186941 1107 56-57 0.4417747907837182 1107 58-59 0.49681978971527485 1107 60-61 0.5728101055538417 1107 62-63 0.6006530594964943 1107 64-65 0.5646112295476797 1107 66-67 0.4117773929451687 1107 68-69 0.6477417662455522 1107 70-71 0.6924698113895076 1107 72-73 0.6832190688049913 1107 74-75 0.7505996286552481 1107 76-77 0.7324773180043103 1107 78-79 0.8063676611426196 1107 80-81 0.8453685453008184 1107 82-83 0.8658881155746911 1107 84-85 0.8806479986339149 1107 86-87 0.8756112280725681 1107 88-89 1.173192287881779 1107 90-91 1.1114551342251957 1107 92-93 1.0700617963449233 1107 94-95 1.1607802503920688 1107 96-97 1.2530124651519472 1107 98-99 1.2872605014354441 1107 100-101 0.8697467243422956 1108 1 -0.03140076894077737 1108 2 -0.13812296920858458 1108 3 0.020550133819135397 1108 4 0.1057552172272338 1108 5 0.05770488177767419 1108 6 -0.03070305745677615 1108 7 -0.008411808220131434 1108 8 0.10591024487776224 1108 9 0.0942876111581441 1108 10-11 0.12984860409870436 1108 12-13 0.11256984861881136 1108 14-15 0.27758378872137257 1108 16-17 0.1592833725247047 1108 18-19 0.08414216563182464 1108 20-21 0.18641363628466934 1108 22-23 0.17372934028080067 1108 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-1.1551902123442268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 8.0 5 30.0 6 77.0 7 184.0 8 440.0 9 846.0 10 1451.0 11 2209.0 12 3388.0 13 4525.0 14 5907.0 15 8022.0 16 10725.0 17 14351.0 18 12978.0 19 8496.0 20 7352.0 21 7591.0 22 7829.0 23 9050.0 24 10618.0 25 12260.0 26 15153.0 27 18729.0 28 23714.0 29 28839.0 30 36033.0 31 44389.0 32 56030.0 33 71684.0 34 95731.0 35 132930.0 36 197745.0 37 292658.0 38 217606.0 39 17564.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.88436121668309 20.350267377737236 15.71569572319087 22.049675682388802 2 15.23827573587076 21.381133708602732 40.20625294086893 23.17433761465758 3 18.84381898454746 25.902680957360285 30.421459277332403 24.832040780759847 4 12.87790001089206 18.00152488835639 37.71528155974295 31.405293541008604 5 13.463124813017199 38.045154712837984 34.14901403787 14.342706436274808 6 30.55332670608644 37.586728466107004 17.693224305682126 14.166720522124427 7 27.26585537824102 33.67546478200285 21.363307291647757 17.695372548108377 8 25.886994236652335 35.29228094813132 20.068460176117217 18.752264639099124 9 26.293122540665475 17.03182875370965 20.795468325550356 35.879580380074515 10-11 24.324638329420665 27.604961256971823 27.503701482263022 20.566698931344494 12-13 25.500111462644035 24.883399908361007 28.19870558104714 21.417783047947818 14-15 22.62587762707212 26.0594517231589 26.77142884113652 24.54324180863246 16-17 21.37764849315725 29.30953105360269 27.370963677911863 21.941856775328194 18-19 21.46514797578479 27.60056812942081 30.133696304907744 20.800587589886657 20-21 22.432212919020756 26.696642169970836 30.203369412133764 20.667775498874647 22-23 22.040865526002595 26.518696933016063 30.221776696963516 21.21866084401783 24-25 21.874714074297838 26.77150488052481 30.235508377713842 21.118272667463504 26-27 21.676228277606587 26.96029362696029 30.126208907492913 21.237269187940207 28-29 21.50861284896262 27.127015876485324 30.116596513178607 21.247774761373453 30-31 21.780300073095564 26.873096583099997 30.095547125579493 21.251056218224942 32-33 21.671616538623525 26.93709426783043 29.994590237739082 21.39669895580696 34-35 21.868984262048905 26.922476448987272 29.94157359178632 21.266965697177508 36-37 21.992593130491613 26.813630092222784 29.918415510856143 21.275361266429453 38-39 21.80688027593719 27.08541345193791 29.826776799491693 21.280929472633204 40-41 21.842845664271653 26.962813480662422 29.83297854147688 21.36136231358904 42-43 21.659702285882958 27.10698690352702 29.60969816498339 21.62361264560663 44-45 21.9882880845111 27.14943856654113 29.393574893556924 21.468698455390847 46-47 22.14295620880529 27.3913715945043 28.862563164915343 21.603109031775066 48-49 22.231516789946763 27.493077180855952 28.577709666023672 21.697696363173616 50-51 22.13823631284632 27.691883739522737 28.473462203436227 21.69641774419471 52-53 22.347635206032216 27.642888626347812 28.313263947938445 21.69621221968153 54-55 22.1850023400599 27.705909840342198 28.212335837263993 21.89675198233391 56-57 22.196440248005565 27.97512828155901 28.12551170118793 21.702919769247497 58-59 22.310283243116068 27.709268167290364 28.21303013458704 21.767418455006524 60-61 22.24135207994844 27.624583601855296 28.539415998765556 21.594648319430704 62-63 22.021114605442683 27.597699889118914 28.744741862003515 21.636443643434887 64-65 22.062842918843874 27.58882850644956 28.543954607638394 21.804373967068173 66-67 22.04846966561377 27.76062883491268 28.23643030897143 21.954471190502122 68-69 22.043407004751113 27.682476358801612 28.370708803900225 21.903407832547046 70-71 22.089121107854382 27.600727154696823 28.338193012590875 21.97195872485792 72-73 22.11758187736857 27.561756118247015 28.197452566929844 22.123209437454573 74-75 22.02364743923488 27.95707357311892 27.879231483639728 22.14004750400647 76-77 22.172126579401493 27.781128110469243 28.008664311308152 22.038080998821112 78-79 22.13160822220996 27.852598268305222 27.8423234104105 22.17347009907432 80-81 22.102180717029636 27.899707349662688 27.846187920730248 22.151924012577428 82-83 22.206852081395425 27.869030424891207 27.818127423066397 22.10599007064698 84-85 22.133541500254157 27.93166799796674 27.646394597342237 22.28839590443686 86-87 21.99203705102213 27.804532331054194 27.769168879000354 22.434261738923325 88-89 22.143931579618098 27.692413262766465 27.667143381524014 22.496511776091424 90-91 22.06164905783684 27.814435609773806 27.78295755727408 22.340957775115275 92-93 21.943921826904713 27.790965247769122 27.707423089295347 22.557689836030814 94-95 22.014950435700385 27.81006868893414 27.61517350500181 22.55980737036366 96-97 22.092576590445347 27.85357954586713 27.458409881420053 22.59543398226747 98-99 21.88167838322401 27.85995464417901 27.353749988199933 22.904616984397048 100-101 21.80496006756038 27.864844385659104 27.337264915620324 22.992930631160192 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 341.0 1 244.0 2 169.0 3 216.0 4 212.5 5 198.5 6 324.5 7 723.0 8 1281.0 9 1601.5 10 1506.0 11 1403.5 12 1495.0 13 1619.5 14 1792.5 15 1954.0 16 2089.5 17 2165.5 18 2286.5 19 2531.5 20 2799.5 21 3218.5 22 3825.0 23 4642.5 24 5603.0 25 6888.0 26 8863.5 27 11253.5 28 13280.5 29 15484.0 30 18116.0 31 20841.5 32 23691.5 33 26515.5 34 29938.0 35 33085.0 36 36598.5 37 40098.5 38 42104.5 39 42946.0 40 43656.5 41 45076.5 42 45790.5 43 47192.5 44 49746.5 45 51209.5 46 51257.0 47 51192.5 48 52142.0 49 51334.0 50 48967.0 51 47275.5 52 45809.5 53 44871.0 54 43493.5 55 39972.0 56 35546.5 57 31666.0 58 28995.5 59 26112.5 60 21594.5 61 16876.5 62 12825.0 63 9543.0 64 6787.0 65 4722.0 66 3268.5 67 2387.0 68 1639.5 69 980.0 70 590.5 71 334.5 72 195.5 73 133.5 74 82.0 75 47.0 76 32.5 77 14.0 78 4.0 79 2.5 80 1.5 81 1.5 82 2.0 83 1.0 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0188795564175606 2 5.082957497035546E-4 3 0.0022510240344014564 4 7.261367852907924E-5 5 0.0013796598920525055 6 5.809094282326339E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.809094282326339E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0019242624810205998 22-23 0.0 24-25 0.003267615533808566 26-27 0.0021421035166078375 28-29 0.0037396044442475807 30-31 0.013542451045673278 32-33 7.987504638198716E-4 34-35 0.002468865069988694 36-37 0.003703297604983041 38-39 0.0018879556417560604 40-41 0.0038122181227766595 42-43 0.0017790351239624412 44-45 4.719889104390151E-4 46-47 1.4522735705815848E-4 48-49 1.815341963226981E-4 50-51 1.4522735705815848E-4 52-53 8.713641423489509E-4 54-55 0.003594377087189422 56-57 3.2676155338085656E-4 58-59 9.076709816134905E-4 60-61 9.802846601425696E-4 62-63 0.0 64-65 2.1784103558723772E-4 66-67 7.261367852907924E-5 68-69 0.0 70-71 1.815341963226981E-4 72-73 0.0 74-75 2.9045471411631696E-4 76-77 5.446025889680943E-4 78-79 2.1784103558723772E-4 80-81 0.005881707960855418 82-83 0.0015975009276397432 84-85 0.003703297604983041 86-87 0.0016701146061688225 88-89 0.0012707393742588866 90-91 7.261367852907924E-5 92-93 1.4522735705815848E-4 94-95 3.630683926453962E-5 96-97 0.001524887249110664 98-99 0.0029045471411631696 100-101 9.076709816134905E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1377151.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.537359170165175 #Duplication Level Percentage of deduplicated Percentage of total 1 79.66919910712578 49.026321202546015 2 12.066172230822021 14.850407487543357 3 3.5624891645227335 6.576785257711713 4 1.5227377524428773 3.7482103997618963 5 0.8173764416631097 2.5149593833927177 6 0.5090398369788474 1.879498036805379 7 0.3422926050528356 1.4744648084899485 8 0.24435491174692128 1.2029564775331447 9 0.1818329631567237 1.0070568326475646 >10 0.996511922396627 11.209080483236582 >50 0.06133485918272127 2.577811502971729 >100 0.025832526524739073 2.6133461950934143 >500 3.538621645689113E-4 0.15409967546369638 >1k 2.3590810971260752E-4 0.3376691899468279 >5k 2.3590810971260752E-4 0.8273330668559639 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6286 0.4564495832337921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5112 0.371201124640653 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3401 0.24695912067739847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.261367852907924E-5 2 0.0 0.0 0.0 0.0 7.261367852907924E-5 3 0.0 0.0 0.0 0.0 7.261367852907924E-5 4 0.0 0.0 0.0 0.0 7.261367852907924E-5 5 0.0 0.0 0.0 0.0 7.261367852907924E-5 6 0.0 0.0 0.0 0.0 7.261367852907924E-5 7 0.0 0.0 0.0 0.0 7.261367852907924E-5 8 0.0 0.0 0.0 0.0 7.261367852907924E-5 9 0.0 0.0 0.0 0.0 7.261367852907924E-5 10-11 7.261367852907924E-5 0.0 0.0 0.0 7.261367852907924E-5 12-13 1.4522735705815848E-4 3.630683926453962E-5 0.0 0.0 1.4522735705815848E-4 14-15 1.4522735705815848E-4 7.261367852907924E-5 0.0 0.0 1.4522735705815848E-4 16-17 1.4522735705815848E-4 7.261367852907924E-5 0.0 0.0 1.4522735705815848E-4 18-19 1.4522735705815848E-4 7.261367852907924E-5 0.0 0.0 2.1784103558723772E-4 20-21 1.4522735705815848E-4 7.261367852907924E-5 0.0 3.630683926453962E-5 2.1784103558723772E-4 22-23 1.4522735705815848E-4 7.261367852907924E-5 0.0 1.815341963226981E-4 2.1784103558723772E-4 24-25 3.267615533808566E-4 7.261367852907924E-5 0.0 4.3568207117447545E-4 2.1784103558723772E-4 26-27 3.630683926453962E-4 7.261367852907924E-5 0.0 8.350573030844112E-4 2.541478748517773E-4 28-29 7.261367852907924E-4 7.261367852907924E-5 0.0 0.0016338077669042828 2.9045471411631696E-4 30-31 7.261367852907924E-4 7.261367852907924E-5 0.0 0.0033039223730731053 3.267615533808566E-4 32-33 7.261367852907924E-4 7.261367852907924E-5 0.0 0.005772787443061799 3.630683926453962E-4 34-35 7.261367852907924E-4 7.261367852907924E-5 0.0 0.008060118316727795 4.3568207117447545E-4 36-37 7.261367852907924E-4 7.261367852907924E-5 0.0 0.010020687637012936 4.3568207117447545E-4 38-39 8.350573030844112E-4 7.261367852907924E-5 0.0 0.012126484314356232 4.3568207117447545E-4 40-41 8.713641423489509E-4 7.261367852907924E-5 0.0 0.01593870243713289 4.3568207117447545E-4 42-43 8.713641423489509E-4 7.261367852907924E-5 0.0 0.022982229254453576 4.3568207117447545E-4 44-45 0.0010892051779361886 7.261367852907924E-5 0.0 0.03289399637367289 4.3568207117447545E-4 46-47 0.001234432534994347 7.261367852907924E-5 0.0 0.04462110545611919 4.3568207117447545E-4 48-49 0.001234432534994347 7.261367852907924E-5 0.0 0.06930975615600612 4.3568207117447545E-4 50-51 0.001234432534994347 7.261367852907924E-5 0.0 0.09338119058839589 4.3568207117447545E-4 52-53 0.001234432534994347 1.0892051779361886E-4 0.0 0.12006671744783252 5.082957497035546E-4 54-55 0.001234432534994347 1.4522735705815848E-4 0.0 0.17289316857773768 5.809094282326339E-4 56-57 0.001234432534994347 1.4522735705815848E-4 0.0 0.2233233683161832 6.172162674971736E-4 58-59 0.001234432534994347 1.4522735705815848E-4 0.0 0.2885667584745609 6.535231067617131E-4 60-61 0.001234432534994347 1.4522735705815848E-4 0.0 0.3623059490208409 6.535231067617131E-4 62-63 0.0013070462135234262 1.4522735705815848E-4 0.0 0.45463424127056506 6.535231067617131E-4 64-65 0.0013070462135234262 1.4522735705815848E-4 0.0 0.5321130362610926 6.535231067617131E-4 66-67 0.0014522735705815848 1.4522735705815848E-4 0.0 0.6354059939687078 6.898299460262528E-4 68-69 0.0014522735705815848 1.4522735705815848E-4 0.0 0.7782007927961422 7.261367852907924E-4 70-71 0.0014522735705815848 1.4522735705815848E-4 0.0 0.8741234621330558 7.261367852907924E-4 72-73 0.0014522735705815848 1.4522735705815848E-4 0.0 1.005263765556573 7.261367852907924E-4 74-75 0.0014522735705815848 1.4522735705815848E-4 0.0 1.1654858472309861 7.261367852907924E-4 76-77 0.0014522735705815848 1.4522735705815848E-4 0.0 1.3009466645269838 7.261367852907924E-4 78-79 0.0014522735705815848 1.4522735705815848E-4 0.0 1.424099463312302 7.261367852907924E-4 80-81 0.0014522735705815848 1.4522735705815848E-4 0.0 1.598699053335473 7.62443624555332E-4 82-83 0.0014522735705815848 1.4522735705815848E-4 0.0 1.8379974309280538 8.713641423489509E-4 84-85 0.0015611940883752035 1.4522735705815848E-4 0.0 2.1245310064038003 9.076709816134905E-4 86-87 0.0016701146061688225 1.4522735705815848E-4 0.0 2.440001132773385 9.439778208780301E-4 88-89 0.0016701146061688225 1.4522735705815848E-4 0.0 2.7568509190350223 9.439778208780301E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4290 0.0 40.745605 1 GTATCAA 6950 0.0 35.334263 1 TATCAAC 8680 0.0 28.236088 2 ATCAACG 8675 0.0 28.197609 3 TCAACGC 8720 0.0 28.052094 4 CAACGCA 8890 0.0 27.62252 5 AACGCAG 9190 0.0 26.720806 6 TTACGTG 95 0.0012269926 24.998901 5 ACGCAGA 10580 0.0 22.985758 7 CGCAGAG 10670 0.0 22.747362 8 GAGTACT 5915 0.0 22.323212 12-13 GTACTTT 6125 0.0 21.326368 14-15 GCAGAGT 11225 0.0 21.114885 9 GTACATG 6050 0.0 20.727114 1 TACATGG 6125 0.0 20.084831 2 AGAGTAC 9590 0.0 19.786669 10-11 AGTACTT 6130 0.0 19.448221 12-13 ACATGGG 6235 0.0 19.27341 3 TAGCGGT 150 7.7244017E-4 18.999165 4 ACTTTTT 7115 0.0 18.19206 16-17 >>END_MODULE