FastQCFastQC Report
Thu 26 May 2016
SRR937134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952732
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42290.4438813853213705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30200.3169831600072213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18410.1932337740308922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.043.582541
GTATCAA41350.039.30071
ATCAACG50500.031.9026263
TATCAAC50850.031.8682882
TCAACGC51300.031.4051234
CAACGCA52250.030.838985
AACGCAG53850.030.0109566
GTACCGT1004.929504E-528.5192346
ACGCAGA61550.025.8704137
CGCAGAG61600.025.6206198
TACATGG39700.024.6588042
GTACATG39700.024.2971421
GCAGAGT65250.023.6046079
ACATGGG40800.023.0633933
GAGTACT34700.021.7136112-13
TAACGCG550.00287556121.59413376-77
AGTACTT36600.020.78122512-13
AGAGTAC57700.020.18471110-11
GTACTTT38000.019.88841214-15
CAGAGTA64850.018.94884910-11
Produced by FastQC (version 0.11.5)