##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937134_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 952732 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.676592158130514 33.0 31.0 34.0 30.0 34.0 2 31.78067179437659 33.0 31.0 34.0 30.0 34.0 3 31.792111527690892 33.0 31.0 34.0 30.0 34.0 4 35.379951549858724 37.0 35.0 37.0 33.0 37.0 5 35.38664703190404 37.0 35.0 37.0 33.0 37.0 6 35.44678776403018 37.0 35.0 37.0 33.0 37.0 7 35.38578739876481 37.0 35.0 37.0 33.0 37.0 8 35.33472057199716 37.0 35.0 37.0 33.0 37.0 9 36.95697111044869 39.0 37.0 39.0 33.0 39.0 10-11 36.79084621908365 39.0 37.0 39.0 32.5 39.0 12-13 36.73153940457547 39.0 37.0 39.0 32.0 39.0 14-15 37.864999286263085 40.0 38.0 41.0 32.5 41.0 16-17 37.792908184043355 40.0 38.0 41.0 32.0 41.0 18-19 37.77810811434905 40.0 37.5 41.0 32.0 41.0 20-21 37.723433242506815 40.0 37.5 41.0 32.0 41.0 22-23 37.606631245722824 40.0 37.0 41.0 32.0 41.0 24-25 37.45609625791933 40.0 37.0 41.0 32.0 41.0 26-27 37.53716837473707 40.0 37.0 41.0 32.0 41.0 28-29 37.47586886973461 40.0 37.0 41.0 32.0 41.0 30-31 37.32418245634659 40.0 37.0 41.0 31.5 41.0 32-33 37.11789726806699 40.0 36.0 41.0 31.0 41.0 34-35 36.96140520104289 39.0 36.0 41.0 30.5 41.0 36-37 36.77516394956819 39.0 36.0 41.0 30.0 41.0 38-39 36.63426336052531 39.0 35.5 41.0 30.0 41.0 40-41 36.46272613914511 39.0 35.0 41.0 30.0 41.0 42-43 36.24373433452429 39.0 35.0 41.0 30.0 41.0 44-45 35.98432927622878 39.0 35.0 40.5 29.0 41.0 46-47 35.62265516430644 39.0 35.0 40.0 27.5 41.0 48-49 35.29984822594392 39.0 35.0 40.0 26.0 41.0 50-51 34.655272416587245 38.0 34.0 39.5 25.0 40.5 52-53 34.88178837280579 38.5 34.5 40.0 26.0 40.5 54-55 35.291213583673056 39.0 35.0 40.5 26.0 41.0 56-57 35.18907258284597 39.0 35.0 41.0 26.0 41.0 58-59 34.95383329204855 38.5 34.5 40.5 26.0 41.0 60-61 34.69441826242847 38.0 34.0 40.0 25.5 41.0 62-63 34.38371598728708 38.0 34.0 40.0 24.5 41.0 64-65 34.082901592473014 37.5 34.0 40.0 23.5 41.0 66-67 33.69644768938169 37.0 33.5 40.0 22.5 41.0 68-69 33.317711591507376 36.0 33.0 39.0 22.0 41.0 70-71 32.90078899417675 36.0 32.5 39.0 21.5 41.0 72-73 32.51128071692774 35.0 32.0 38.5 20.0 40.0 74-75 32.08982851420966 35.0 32.0 37.5 19.5 40.0 76-77 31.650665664636016 35.0 31.5 37.0 18.0 39.0 78-79 31.207372587464263 35.0 31.0 36.5 15.0 39.0 80-81 30.778865935016356 35.0 31.0 36.0 11.0 38.5 82-83 30.37032607280956 34.5 30.5 36.0 7.0 37.0 84-85 30.01420283983324 34.0 30.0 35.0 6.5 37.0 86-87 29.683743172266702 34.0 30.0 35.0 2.0 36.5 88-89 29.324613847335872 34.0 29.5 35.0 2.0 36.0 90-91 28.976291863818997 34.0 29.0 35.0 2.0 36.0 92-93 28.605121377260343 34.0 29.0 35.0 2.0 35.0 94-95 28.188758223718736 34.0 29.0 35.0 2.0 35.0 96-97 27.695397026655975 34.0 28.0 35.0 2.0 35.0 98-99 27.096008111410136 34.0 26.5 35.0 2.0 35.0 100-101 24.970783494204035 31.5 21.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4607709051933675 1101 2 0.4556541941975105 1101 3 0.41742329677476064 1101 4 0.4107699235189628 1101 5 0.3492903932802349 1101 6 0.27594007575680024 1101 7 0.2555935678760264 1101 8 0.2616102895895054 1101 9 0.26425856114034474 1101 10-11 0.3902061018309624 1101 12-13 0.41580339373026476 1101 14-15 0.7093349843321874 1101 16-17 0.6652044260638164 1101 18-19 0.5940005307934442 1101 20-21 0.6224448342952869 1101 22-23 0.6360502550505842 1101 24-25 0.6368856652345869 1101 26-27 0.7323880922216617 1101 28-29 0.7878076773096012 1101 30-31 0.6161149299928752 1101 32-33 0.793388434093913 1101 34-35 0.7813458954019694 1101 36-37 0.5770273874912917 1101 38-39 0.6263483467996309 1101 40-41 0.7171203760183431 1101 42-43 0.8458996741018723 1101 44-45 0.9222497618943706 1101 46-47 1.0464060734644676 1101 48-49 1.1698010245565982 1101 50-51 0.9078985964287511 1101 52-53 0.9039038205424887 1101 54-55 0.9342740441490278 1101 56-57 0.9679779804719999 1101 58-59 0.8723453301136743 1101 60-61 0.8765914841676974 1101 62-63 0.942503135556791 1101 64-65 1.0612882701528292 1101 66-67 0.8579128374360465 1101 68-69 0.8182791666832827 1101 70-71 0.88478149991424 1101 72-73 0.8909072797438142 1101 74-75 0.8968669275875136 1101 76-77 0.9999602962194594 1101 78-79 0.9813167215080263 1101 80-81 0.9335243473845161 1101 82-83 0.8467444330653358 1101 84-85 0.8026205806027278 1101 86-87 0.9151280469191185 1101 88-89 0.9696340930498941 1101 90-91 0.956568746596016 1101 92-93 1.100881085755958 1101 94-95 1.0718021491462792 1101 96-97 1.0007172885511082 1101 98-99 1.025216208604288 1101 100-101 0.8720758205920802 1102 1 0.4804079281730047 1102 2 0.3977322151597811 1102 3 0.369849639893264 1102 4 0.3981274737437346 1102 5 0.3373835739632014 1102 6 0.21865391076521945 1102 7 0.3228822035300354 1102 8 0.28726537604001123 1102 9 0.3972171154687203 1102 10-11 0.5548046026874474 1102 12-13 0.5401588663712928 1102 14-15 0.5706020299437569 1102 16-17 0.6125384823099509 1102 18-19 0.4825944846411332 1102 20-21 0.5567191297955887 1102 22-23 0.5348480653589789 1102 24-25 0.6367872924483322 1102 26-27 0.49445381349216433 1102 28-29 0.5269963637379931 1102 30-31 0.4603459538411556 1102 32-33 0.6279369566254047 1102 34-35 0.6512555161779758 1102 36-37 0.5169670893041953 1102 38-39 0.6688989077307426 1102 40-41 0.5814545077454838 1102 42-43 0.6706022562534599 1102 44-45 0.5065148181895864 1102 46-47 0.7533010571123171 1102 48-49 1.1011036163598575 1102 50-51 1.031012860235279 1102 52-53 1.0280261476177728 1102 54-55 0.9311766418917742 1102 56-57 1.1263217663435299 1102 58-59 1.2567876224099521 1102 60-61 0.9172031525412834 1102 62-63 0.8474876042055683 1102 64-65 0.9540628611839495 1102 66-67 0.9354402859181974 1102 68-69 0.8674234884286989 1102 70-71 0.9561721982620313 1102 72-73 0.9939414305680572 1102 74-75 0.8085137292758127 1102 76-77 0.872956595175399 1102 78-79 0.7199055814189954 1102 80-81 0.7576756707150345 1102 82-83 0.6044477234184917 1102 84-85 0.8243224009323598 1102 86-87 0.6010982933790388 1102 88-89 0.7207624928401977 1102 90-91 0.9204286060033589 1102 92-93 0.9392242818032273 1102 94-95 0.7687247720411143 1102 96-97 0.8102164287688325 1102 98-99 0.8739159760980435 1102 100-101 0.7586797362826836 1103 1 0.28493183619430695 1103 2 0.28937110280401157 1103 3 0.27482089524804465 1103 4 0.17915183974116644 1103 5 0.24554138451426155 1103 6 0.044208249450811365 1103 7 0.02742559768024222 1103 8 0.14260900402375398 1103 9 0.10638507860555535 1103 10-11 0.20769646926060403 1103 12-13 0.1423568391886647 1103 14-15 0.38485701896587443 1103 16-17 0.3647416132202679 1103 18-19 0.2704939633090362 1103 20-21 0.44200079814167026 1103 22-23 0.4916081369262173 1103 24-25 0.5353513106339989 1103 26-27 0.4002563554420462 1103 28-29 0.35638696892296906 1103 30-31 0.23170176117648822 1103 32-33 0.41264128327544825 1103 34-35 0.5043913240760034 1103 36-37 0.42358424522552696 1103 38-39 0.43976886439234164 1103 40-41 0.33475308213195376 1103 42-43 0.3409409814438149 1103 44-45 0.49454231134599524 1103 46-47 0.5888217170981207 1103 48-49 0.5607837416546246 1103 50-51 0.47909431016999093 1103 52-53 0.7085325681409387 1103 54-55 0.7972188840678882 1103 56-57 0.717280046228943 1103 58-59 0.7743801059743944 1103 60-61 0.6794434634023503 1103 62-63 0.8913063852908962 1103 64-65 0.7391532632755329 1103 66-67 0.7803382546716122 1103 68-69 0.8064800992802788 1103 70-71 0.7460348783654425 1103 72-73 0.7238394566336268 1103 74-75 0.6125382401166739 1103 76-77 0.7549363025083302 1103 78-79 0.7999036953284424 1103 80-81 0.7127442525104009 1103 82-83 0.8424606280292046 1103 84-85 0.6199772546978188 1103 86-87 0.7267494374857364 1103 88-89 0.7487876101477333 1103 90-91 0.8578776112208502 1103 92-93 0.829878963632261 1103 94-95 0.8008909522492544 1103 96-97 0.8996556763333281 1103 98-99 0.9024793065379804 1103 100-101 0.9587413207863129 1104 1 0.48888139034120925 1104 2 0.3066839943743709 1104 3 0.3663459210512663 1104 4 0.40817518593550517 1104 5 0.365006980956295 1104 6 0.3995359688298805 1104 7 0.30402506522576545 1104 8 0.288884111407981 1104 9 0.4363695302261732 1104 10-11 0.5068610207275057 1104 12-13 0.496003842249614 1104 14-15 0.4401630326301387 1104 16-17 0.4722277223010778 1104 18-19 0.48253406424799294 1104 20-21 0.477760963282293 1104 22-23 0.5026167734381843 1104 24-25 0.48213465659208765 1104 26-27 0.4666381899736223 1104 28-29 0.4579235711074219 1104 30-31 0.6287697916117381 1104 32-33 0.5182115307944315 1104 34-35 0.4895904629349914 1104 36-37 0.6701430773104136 1104 38-39 0.5909735315639963 1104 40-41 0.4970339393157346 1104 42-43 0.4941010086719402 1104 44-45 0.3948599235401815 1104 46-47 0.6247055457499613 1104 48-49 0.7215666092010409 1104 50-51 0.5203735593589798 1104 52-53 0.4889829543402371 1104 54-55 0.6783564702447435 1104 56-57 0.5535676454735281 1104 58-59 0.6112827004717758 1104 60-61 0.5414626707450836 1104 62-63 0.7012008689385212 1104 64-65 0.6845958752851473 1104 66-67 0.5530764675087028 1104 68-69 0.6622242140045813 1104 70-71 0.5205630743842917 1104 72-73 0.7758460539896603 1104 74-75 0.7790923176164064 1104 76-77 0.757350140418314 1104 78-79 0.8558498060925608 1104 80-81 0.9430540683662052 1104 82-83 0.6290949280789633 1104 84-85 0.6707091950263013 1104 86-87 0.6222006136549254 1104 88-89 0.6518158526788298 1104 90-91 0.7882480406543912 1104 92-93 0.7986356543819326 1104 94-95 0.7760615340657679 1104 96-97 0.49931997604347345 1104 98-99 0.6448184443043026 1104 100-101 0.6952708575391071 1105 1 0.011398906386229868 1105 2 0.12266896695768637 1105 3 0.3066424498051852 1105 4 0.22833349926174407 1105 5 0.2634829306728079 1105 6 0.15227128071657603 1105 7 0.1339005914335445 1105 8 0.2620818686762192 1105 9 0.21860940469905898 1105 10-11 0.06406648057591013 1105 12-13 0.24156263041897574 1105 14-15 0.32238178009519913 1105 16-17 0.2776237659207865 1105 18-19 0.2382223143627087 1105 20-21 0.3522783413056061 1105 22-23 0.296686864254049 1105 24-25 0.42010933323989974 1105 26-27 0.2893647362708336 1105 28-29 0.2415188430162516 1105 30-31 -0.10670675256670847 1105 32-33 -0.07794117449168425 1105 34-35 0.11999646470021474 1105 36-37 -0.05945301546439197 1105 38-39 -0.046261820881703386 1105 40-41 0.04443198788243308 1105 42-43 0.20443459746650916 1105 44-45 0.1977213315795865 1105 46-47 0.28306487922615986 1105 48-49 0.2760706572325944 1105 50-51 0.172903291536322 1105 52-53 0.133565844248551 1105 54-55 0.20715155279746256 1105 56-57 0.30429528625870006 1105 58-59 0.1637454800111442 1105 60-61 0.1527662858243204 1105 62-63 0.23146848577572143 1105 64-65 0.34540902899653503 1105 66-67 0.22125380603843325 1105 68-69 0.6458762079945899 1105 70-71 0.3092575826940518 1105 72-73 0.30222333513141564 1105 74-75 0.318944876526853 1105 76-77 0.5326592666803371 1105 78-79 0.5849607041175169 1105 80-81 0.4440692543867861 1105 82-83 0.35561549755170674 1105 84-85 0.3048596411148985 1105 86-87 0.5261187353004857 1105 88-89 0.4529105222560581 1105 90-91 0.39494306230805876 1105 92-93 0.34639873405799193 1105 94-95 0.3735499626401406 1105 96-97 0.41877008058886744 1105 98-99 0.3929130787707429 1105 100-101 0.4248678583514973 1106 1 0.05028842282560575 1106 2 0.1484456564140082 1106 3 0.08340848855972638 1106 4 0.034556970628528916 1106 5 0.08842984947436605 1106 6 0.3166363261969707 1106 7 0.24992010463731873 1106 8 0.15305337426769228 1106 9 0.15208416025750182 1106 10-11 0.26050089825266554 1106 12-13 0.3285114522939736 1106 14-15 0.2613522824568122 1106 16-17 0.41922372609791836 1106 18-19 0.5252135437596408 1106 20-21 0.4807357988071388 1106 22-23 0.21394911887914958 1106 24-25 0.36582818381417326 1106 26-27 0.08680324536403106 1106 28-29 0.09537227955716787 1106 30-31 0.19134920238749942 1106 32-33 0.3414874056891648 1106 34-35 0.5633431744317363 1106 36-37 0.488464612471347 1106 38-39 0.5319901085169505 1106 40-41 0.5046737394758196 1106 42-43 0.3776970856680322 1106 44-45 0.24366552596112 1106 46-47 0.35518351307214857 1106 48-49 0.40231966884057613 1106 50-51 0.4675034642297149 1106 52-53 0.44597095382843577 1106 54-55 0.5397294323447923 1106 56-57 0.2833203153121602 1106 58-59 0.49271776291431024 1106 60-61 0.5121963593625978 1106 62-63 0.6059302548681984 1106 64-65 0.7103956366436748 1106 66-67 0.742666254129901 1106 68-69 0.6466745798520748 1106 70-71 0.3632836168919269 1106 72-73 0.5070411359519937 1106 74-75 0.5298548098426998 1106 76-77 0.7346167136517927 1106 78-79 0.564537605324265 1106 80-81 0.7425952340196851 1106 82-83 0.5805907518532472 1106 84-85 0.6356531499879274 1106 86-87 0.9129519177026104 1106 88-89 0.8524204904169146 1106 90-91 0.73744746101152 1106 92-93 0.9870552943963347 1106 94-95 0.901811752026962 1106 96-97 1.158350562024328 1106 98-99 0.8884373142387467 1106 100-101 0.8833319402334325 1107 1 0.21785307659832043 1107 2 0.08779255913784567 1107 3 0.09461037978811504 1107 4 0.058493479389753134 1107 5 0.13107730943514184 1107 6 -0.04744332361403991 1107 7 0.031900179195361034 1107 8 0.07232545916514255 1107 9 0.15499893807713505 1107 10-11 0.11363389473258678 1107 12-13 0.23677908189377916 1107 14-15 0.05531749736321956 1107 16-17 0.036487679752433166 1107 18-19 0.15993563969791325 1107 20-21 0.40027026566518487 1107 22-23 0.16305351312752947 1107 24-25 0.30952062539522274 1107 26-27 0.329237682346907 1107 28-29 0.3239881497690291 1107 30-31 0.2118513650902969 1107 32-33 0.32482874903284653 1107 34-35 0.22342279826433042 1107 36-37 0.35434586515037125 1107 38-39 0.007370367521716048 1107 40-41 0.31254324991265037 1107 42-43 0.4250005265086898 1107 44-45 0.680429525077848 1107 46-47 0.25281558082384237 1107 48-49 0.5618933631006442 1107 50-51 0.43785300376236336 1107 52-53 0.41735378469599027 1107 54-55 0.7207268734712144 1107 56-57 0.725591088299808 1107 58-59 0.7346261370553933 1107 60-61 0.6256843508416807 1107 62-63 0.40200296844321315 1107 64-65 0.4073113496861325 1107 66-67 0.7313721395391255 1107 68-69 0.6509155556012303 1107 70-71 0.45317152004441397 1107 72-73 0.31999397214512015 1107 74-75 0.4577465318217371 1107 76-77 0.6937201986557504 1107 78-79 0.636608864108215 1107 80-81 0.3158023988657028 1107 82-83 0.4037115384795591 1107 84-85 0.5673422663322505 1107 86-87 0.3527625350795702 1107 88-89 0.31887391761890527 1107 90-91 0.49047979114518014 1107 92-93 0.49876831087964035 1107 94-95 0.6642534629651706 1107 96-97 0.7503089446084026 1107 98-99 0.6390932691402256 1107 100-101 0.8014564287834531 1108 1 -0.17002810353789855 1108 2 -0.10455486382828383 1108 3 -0.11912092338394231 1108 4 -0.18727921085388743 1108 5 -0.13325573555482606 1108 6 -0.20241640690048257 1108 7 -0.003384650864020955 1108 8 -0.1390946179317183 1108 9 -0.1667939418012523 1108 10-11 -0.06943083135520567 1108 12-13 0.056064214293776615 1108 14-15 0.05026169032372252 1108 16-17 0.09439934590120203 1108 18-19 0.03350015162776998 1108 20-21 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-1.8882656884626208 2316 80-81 -1.7374124005856721 2316 82-83 -1.2694862001214489 2316 84-85 -1.6300174834419465 2316 86-87 -2.1719815521649366 2316 88-89 -1.8736092724255862 2316 90-91 -1.5560879671928944 2316 92-93 -1.4786344255469501 2316 94-95 -2.14535162852782 2316 96-97 -2.038082986612956 2316 98-99 -2.284003275201261 2316 100-101 -2.5643725278284677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2309.0 3 829.0 4 758.0 5 838.0 6 943.0 7 1020.0 8 1368.0 9 1640.0 10 2022.0 11 2556.0 12 3290.0 13 4016.0 14 4672.0 15 5528.0 16 7045.0 17 9334.0 18 8314.0 19 5573.0 20 5245.0 21 5640.0 22 6189.0 23 6819.0 24 7607.0 25 8851.0 26 10338.0 27 12211.0 28 14373.0 29 17431.0 30 21358.0 31 26754.0 32 33335.0 33 43019.0 34 58034.0 35 82913.0 36 124724.0 37 189384.0 38 180862.0 39 35572.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.042019206740214 19.471657143097147 15.156860645082709 22.329463005079937 2 14.791474832191865 20.412798041214707 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26.544920885369766 30.114806131744825 20.96076953975159 22-23 21.95762779485629 26.418583799492684 30.137569503939254 21.48621890171177 24-25 21.510525335075965 26.734009915151706 30.211818923820243 21.543645825952083 26-27 21.518770086062695 26.831384408990154 30.063157430642562 21.58668807430459 28-29 21.369357547764015 26.944730212051226 30.14696619777451 21.538946042410245 30-31 21.56150277124622 26.76945120927108 30.07342752771246 21.59561849177024 32-33 21.391180394532018 26.84131910949507 29.981273742406888 21.78622675356602 34-35 21.717807897079325 26.814610940360424 29.844088527233335 21.62349263532692 36-37 21.793229335870727 26.67533341345788 29.87812846553971 21.653308785131685 38-39 21.525614272278393 26.98693779181082 29.791470726545967 21.695977209364816 40-41 21.74822157911808 26.735305262782223 29.83721557599633 21.67925758210337 42-43 21.39481009681997 26.889899821139167 29.689218806418836 22.026071275622026 44-45 21.64934440614491 27.000107061436466 29.48582170702113 21.864726825397494 46-47 21.814900282193964 27.17579839059696 29.039475141267545 21.969826185941525 48-49 21.859304921556753 27.26966906574496 28.814025423862237 22.057000588836047 50-51 21.836621587011013 27.44797855322436 28.668057824000403 22.04734203576422 52-53 21.945834227733886 27.485431986283 28.495208024885414 22.073525761097695 54-55 21.841879583784255 27.375590309012793 28.56177159357979 22.220758513623164 56-57 21.84825473577352 27.664979814993963 28.421884735243374 22.064880713989147 58-59 21.892852925353942 27.38210016663824 28.545838636453098 22.17920827155472 60-61 21.871794844877055 27.32128028789576 28.799234069624497 22.007690797602688 62-63 21.674238734275015 27.304371924284922 28.967777423201603 22.053611918238467 64-65 21.69413723316706 27.316051124435266 28.78163786298517 22.208173779412505 66-67 21.640681752333606 27.482721046730855 28.564860109762293 22.31173709117325 68-69 21.623412003943155 27.347861552884563 28.774945225224847 22.253781217947438 70-71 21.751326133945565 27.221517120328876 28.779127444280327 22.248029301445232 72-73 21.70099413178807 27.211024680082723 28.58144637252123 22.506534815607974 74-75 21.567302882523407 27.69006036728462 28.259668029798902 22.482968720393078 76-77 21.641346033719085 27.469011051771254 28.413613615295052 22.476029299214606 78-79 21.685198601442657 27.41823374387081 28.312963954599386 22.58360370008715 80-81 21.642527953013634 27.508841703969722 28.28043359915754 22.568196743859104 82-83 21.819047524054955 27.50567087456671 28.226125237478534 22.449156363899796 84-85 21.762867484956264 27.439961535069855 28.05614578040556 22.74102519956832 86-87 21.672889440391288 27.471380752391344 28.20766794796491 22.648061859252458 88-89 21.7289428971399 27.175731350068055 28.234813066329473 22.860512686462577 90-91 21.734965585910825 27.320582475452436 28.368935412368078 22.57551652626866 92-93 21.74869836643544 27.431107126095984 28.245212119812237 22.574982387656345 94-95 21.688212911790135 27.515645938564724 28.05644977998842 22.739691369656718 96-97 21.748788166901956 27.53287948764122 27.99104768634772 22.727284659109102 98-99 21.672182813670805 27.41149616495196 27.870588723325714 23.045732298051522 100-101 21.88088821296609 27.433322060173467 27.828495327240226 22.857294399620216 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 155.0 1 119.5 2 102.0 3 131.5 4 131.5 5 144.5 6 225.0 7 457.5 8 778.5 9 996.0 10 991.5 11 910.0 12 986.5 13 1091.0 14 1183.0 15 1234.5 16 1312.0 17 1440.0 18 1498.0 19 1603.5 20 1802.0 21 2097.0 22 2515.0 23 3069.0 24 3721.0 25 4632.0 26 6021.5 27 7655.5 28 9082.5 29 10647.0 30 12451.5 31 14336.5 32 16526.0 33 18574.5 34 21078.5 35 23318.0 36 25733.0 37 28150.0 38 29171.5 39 29954.5 40 30630.0 41 31288.0 42 31933.0 43 33224.5 44 34748.5 45 35529.0 46 35772.5 47 35702.5 48 36298.0 49 35808.5 50 34075.5 51 32682.0 52 31680.5 53 30887.5 54 29501.5 55 27132.0 56 24452.0 57 21948.5 58 20005.5 59 17835.5 60 14679.5 61 11554.5 62 8627.5 63 6307.0 64 4626.0 65 3293.0 66 2218.5 67 1523.5 68 1036.0 69 654.0 70 418.5 71 259.0 72 150.0 73 78.5 74 56.5 75 46.0 76 27.5 77 20.0 78 19.0 79 11.0 80 6.0 81 5.5 82 4.0 83 2.5 84 2.0 85 3.0 86 3.5 87 3.0 88 1.0 89 2.0 90 2.5 91 0.5 92 1.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037576149431319615 2 0.03201319993450414 3 0.024036140278693274 4 0.04250933106057107 5 0.062032134955055565 6 0.06665043265052502 7 0.06486609035909363 8 0.06843477494195639 9 0.06623058740548234 10-11 0.07032407854464844 12-13 0.06958934936582375 14-15 0.06266190282261958 16-17 0.05221825235218299 18-19 0.04277173433872275 20-21 0.04980414219318759 22-23 0.028129631417859378 24-25 0.015691716033470065 26-27 0.011808147516825298 28-29 0.01385489308640835 30-31 0.009656440635981577 32-33 0.006192717364379489 34-35 0.007977059655810867 36-37 0.005090623596142462 38-39 0.006297678675640159 40-41 0.006402639986900828 42-43 0.0037786072053840954 44-45 8.396904900853546E-4 46-47 0.0019417842583223826 48-49 5.248065563033465E-4 50-51 0.005038142940512127 52-53 0.004775739662360454 54-55 0.0055629494968154735 56-57 0.01768598094742278 58-59 0.007294811132616517 60-61 0.01731861635801044 62-63 0.011335821616152287 64-65 0.007977059655810867 66-67 0.013435047841365674 68-69 0.020467455695830517 70-71 0.015849158000361067 72-73 0.014904506199015042 74-75 0.015376832099688056 76-77 0.022566681921043902 78-79 0.03379754222593552 80-81 0.029966454364921086 82-83 0.02912676387483574 84-85 0.0184731907818778 86-87 0.01915543930507215 88-89 0.016951251768598095 90-91 0.014956986854645379 92-93 0.028864360596684062 94-95 0.01768598094742278 96-97 0.03699886221938593 98-99 0.05536709169000306 100-101 0.06486609035909363 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 952732.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.44344321269034 #Duplication Level Percentage of deduplicated Percentage of total 1 83.19964749691286 56.11270701273897 2 10.256784911926777 13.835057815046255 3 2.8131628550534846 5.691881677885486 4 1.216529689918077 3.2818780423416647 5 0.638181855086771 2.152059085145701 6 0.41422279666454254 1.6761966998548112 7 0.2716632805568786 1.2825334924647707 8 0.19742569611681343 1.0652054979824135 9 0.1451896139808272 0.8812878737029519 >10 0.7910351340643109 9.580929129487497 >50 0.04060513667921109 1.8785554918151057 >100 0.014929471915718626 1.5276483447595597 >500 1.555152809675268E-4 0.08065645749871375 >1k 4.6654584290258043E-4 0.9534033792760832 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4229 0.4438813853213705 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3020 0.3169831600072213 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1841 0.1932337740308922 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.0496131126066931E-4 0.0 0.0 0.0 6 0.0 1.0496131126066931E-4 0.0 0.0 0.0 7 0.0 1.0496131126066931E-4 0.0 0.0 0.0 8 0.0 1.0496131126066931E-4 0.0 0.0 0.0 9 0.0 1.0496131126066931E-4 0.0 0.0 0.0 10-11 1.0496131126066931E-4 1.0496131126066931E-4 0.0 0.0 0.0 12-13 1.5744196689100397E-4 1.0496131126066931E-4 0.0 0.0 2.6240327815167327E-4 14-15 2.0992262252133862E-4 1.0496131126066931E-4 0.0 0.0 3.1488393378200795E-4 16-17 2.0992262252133862E-4 1.0496131126066931E-4 0.0 1.0496131126066931E-4 3.1488393378200795E-4 18-19 2.0992262252133862E-4 1.0496131126066931E-4 0.0 1.0496131126066931E-4 3.1488393378200795E-4 20-21 2.0992262252133862E-4 1.0496131126066931E-4 0.0 2.0992262252133862E-4 3.1488393378200795E-4 22-23 3.1488393378200795E-4 1.0496131126066931E-4 0.0 2.6240327815167327E-4 3.1488393378200795E-4 24-25 3.1488393378200795E-4 1.0496131126066931E-4 0.0 3.673645894123426E-4 3.1488393378200795E-4 26-27 3.1488393378200795E-4 1.0496131126066931E-4 0.0 7.872098344550199E-4 4.7232590067301187E-4 28-29 3.1488393378200795E-4 1.0496131126066931E-4 0.0 0.0019942649139527173 5.248065563033465E-4 30-31 3.1488393378200795E-4 1.0496131126066931E-4 0.0 0.00461829769546945 5.248065563033465E-4 32-33 3.1488393378200795E-4 1.0496131126066931E-4 0.0 0.0077671370332895295 5.248065563033465E-4 34-35 3.1488393378200795E-4 1.0496131126066931E-4 0.0 0.010706053748588271 5.248065563033465E-4 36-37 3.1488393378200795E-4 1.0496131126066931E-4 0.0 0.013225125218844334 5.248065563033465E-4 38-39 3.1488393378200795E-4 1.0496131126066931E-4 0.0 0.01700373242422843 5.248065563033465E-4 40-41 3.673645894123426E-4 1.0496131126066931E-4 0.0 0.02309148847734725 5.248065563033465E-4 42-43 4.7232590067301187E-4 1.0496131126066931E-4 0.0 0.033377696980892844 5.248065563033465E-4 44-45 6.297678675640159E-4 1.0496131126066931E-4 0.0 0.048124761213016884 5.248065563033465E-4 46-47 6.297678675640159E-4 1.0496131126066931E-4 0.0 0.06360655462396561 5.248065563033465E-4 48-49 6.297678675640159E-4 1.0496131126066931E-4 0.0 0.0961445611147731 5.248065563033465E-4 50-51 6.297678675640159E-4 1.0496131126066931E-4 0.0 0.1300995453075996 6.297678675640159E-4 52-53 6.297678675640159E-4 1.5744196689100397E-4 0.0 0.16568142982496653 6.297678675640159E-4 54-55 6.297678675640159E-4 2.0992262252133862E-4 0.0 0.23479845329011728 6.297678675640159E-4 56-57 6.297678675640159E-4 2.0992262252133862E-4 0.0 0.3060147029804814 6.297678675640159E-4 58-59 6.297678675640159E-4 2.0992262252133862E-4 0.0 0.4028415126184488 6.297678675640159E-4 60-61 6.297678675640159E-4 3.1488393378200795E-4 0.0 0.5088524369917249 6.822485231943506E-4 62-63 6.297678675640159E-4 3.1488393378200795E-4 0.0 0.6483460196571544 7.347291788246852E-4 64-65 6.297678675640159E-4 3.1488393378200795E-4 0.0 0.7577681866464021 7.347291788246852E-4 66-67 6.297678675640159E-4 4.1984524504267725E-4 0.0 0.8985213050469596 7.872098344550199E-4 68-69 6.297678675640159E-4 4.1984524504267725E-4 0.0 1.0863495715479274 8.396904900853545E-4 70-71 7.347291788246852E-4 4.1984524504267725E-4 0.0 1.2182334591469584 8.921711457156892E-4 72-73 7.872098344550199E-4 4.1984524504267725E-4 0.0 1.3953031912437075 9.971324569763584E-4 74-75 8.396904900853545E-4 4.1984524504267725E-4 0.0 1.6162992321030467 0.001049613112606693 76-77 8.396904900853545E-4 4.1984524504267725E-4 0.0 1.7915846219083646 0.001049613112606693 78-79 8.396904900853545E-4 4.1984524504267725E-4 0.0 1.9518080635477761 0.001049613112606693 80-81 9.971324569763584E-4 4.1984524504267725E-4 0.0 2.1740636401422435 0.001049613112606693 82-83 0.001049613112606693 4.1984524504267725E-4 0.0 2.4958225398118254 0.001049613112606693 84-85 0.0012070550794976971 4.1984524504267725E-4 0.0 2.879613574436463 0.001049613112606693 86-87 0.0012595357351280318 4.1984524504267725E-4 0.0 3.285866329670883 0.001049613112606693 88-89 0.0012595357351280318 4.1984524504267725E-4 0.0 3.6942707917861477 0.001049613112606693 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2355 0.0 43.58254 1 GTATCAA 4135 0.0 39.3007 1 ATCAACG 5050 0.0 31.902626 3 TATCAAC 5085 0.0 31.868288 2 TCAACGC 5130 0.0 31.405123 4 CAACGCA 5225 0.0 30.83898 5 AACGCAG 5385 0.0 30.010956 6 GTACCGT 100 4.929504E-5 28.519234 6 ACGCAGA 6155 0.0 25.870413 7 CGCAGAG 6160 0.0 25.620619 8 TACATGG 3970 0.0 24.658804 2 GTACATG 3970 0.0 24.297142 1 GCAGAGT 6525 0.0 23.604607 9 ACATGGG 4080 0.0 23.063393 3 GAGTACT 3470 0.0 21.71361 12-13 TAACGCG 55 0.002875561 21.594133 76-77 AGTACTT 3660 0.0 20.781225 12-13 AGAGTAC 5770 0.0 20.184711 10-11 GTACTTT 3800 0.0 19.888412 14-15 CAGAGTA 6485 0.0 18.948849 10-11 >>END_MODULE