FastQCFastQC Report
Thu 26 May 2016
SRR937134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952732
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45980.4826121091765575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38070.39958771196936815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24340.25547583160846915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51400.036.7018171
GGTATCA30800.036.2557871
ATCCCGT600.003954367731.6605266
AATACCG650.005844617329.2297045
TATCAAC65050.028.994272
ATCAACG64700.028.9277923
TCAACGC65000.028.7912584
CAACGCA66100.028.312135
AACGCAG68050.027.5662946
GGACCGA1351.3444336E-524.6248516
ACGCAGA75650.024.5458017
CGCAGAG76350.023.947558
GAGTACT42300.022.11920212-13
GCAGAGT81500.022.0264039
AGTACTT43900.021.25894212-13
GTACATG42550.021.10681
GTACTTT46100.020.50466714-15
TACATGG42000.020.473792
AGAGTAC69250.020.47075810-11
ACATGGG41500.020.0314943
Produced by FastQC (version 0.11.5)