##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937134_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 952732 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.080832805028066 31.0 31.0 34.0 30.0 34.0 2 32.15704101468199 33.0 31.0 34.0 30.0 34.0 3 32.28756565330019 34.0 31.0 34.0 30.0 34.0 4 35.989293946251415 37.0 35.0 37.0 35.0 37.0 5 35.88327042652078 37.0 35.0 37.0 35.0 37.0 6 35.91286846668318 37.0 35.0 37.0 35.0 37.0 7 35.863076919847344 37.0 35.0 37.0 35.0 37.0 8 35.83666025702926 37.0 35.0 37.0 35.0 37.0 9 37.507750343223485 39.0 37.0 39.0 35.0 39.0 10-11 37.410077545416755 39.0 37.0 39.0 34.0 39.0 12-13 37.32934760247373 39.0 37.0 39.0 34.0 39.0 14-15 38.52364253536147 40.0 38.0 41.0 34.0 41.0 16-17 38.45159919053837 40.0 38.0 41.0 34.0 41.0 18-19 38.488768614888556 40.0 38.0 41.0 33.5 41.0 20-21 38.55781898792105 40.0 38.0 41.0 34.0 41.0 22-23 38.49823140190526 40.0 38.0 41.0 34.0 41.0 24-25 38.407041539488546 40.0 38.0 41.0 34.0 41.0 26-27 38.21244116918504 40.0 38.0 41.0 33.5 41.0 28-29 38.054975585999 40.0 37.5 41.0 33.0 41.0 30-31 38.02462287400864 40.0 37.0 41.0 33.0 41.0 32-33 37.93633676626796 40.0 37.0 41.0 33.0 41.0 34-35 37.81240789644937 40.0 37.0 41.0 33.0 41.0 36-37 37.64851343294862 40.0 37.0 41.0 33.0 41.0 38-39 37.50580016206027 40.0 37.0 41.0 33.0 41.0 40-41 37.29713287682161 40.0 36.0 41.0 32.0 41.0 42-43 37.06928653598284 40.0 36.0 41.0 31.0 41.0 44-45 36.79499638933089 39.0 36.0 41.0 31.0 41.0 46-47 36.41958389137764 39.0 36.0 41.0 30.0 41.0 48-49 36.03853024775068 39.0 35.0 41.0 29.5 41.0 50-51 35.802092298778675 39.0 35.0 40.0 28.5 41.0 52-53 35.57544251688827 39.0 35.0 40.0 28.0 41.0 54-55 35.30907380039717 38.5 35.0 40.0 27.0 41.0 56-57 35.05203561967059 38.0 34.0 40.0 26.5 41.0 58-59 34.71474192112787 38.0 34.0 40.0 26.0 41.0 60-61 34.37696907419925 38.0 34.0 40.0 26.0 41.0 62-63 34.04246419769673 37.0 33.5 40.0 25.0 41.0 64-65 33.63979114798285 36.5 33.0 39.5 23.5 41.0 66-67 33.204242116355914 36.0 32.5 39.0 22.5 40.0 68-69 32.76272026131168 35.5 32.0 39.0 22.0 40.0 70-71 32.36716148927505 35.0 31.5 38.0 21.0 40.0 72-73 31.98454707094965 35.0 31.0 37.5 20.0 39.5 74-75 31.501263734187578 35.0 31.0 37.0 18.5 39.0 76-77 30.0300603947385 33.5 29.5 35.5 17.5 38.0 78-79 30.710287887884526 34.0 30.0 36.0 17.5 38.5 80-81 30.28414916261866 34.0 30.0 36.0 9.5 37.5 82-83 30.18950607306147 34.0 30.0 35.0 11.5 37.0 84-85 29.875558394175908 34.0 30.0 35.0 7.5 36.5 86-87 29.523330275460467 34.0 29.5 35.0 6.0 36.0 88-89 29.145259632299535 34.0 29.0 35.0 2.0 36.0 90-91 28.687835088986198 34.0 29.0 35.0 2.0 35.0 92-93 28.254638765151164 33.5 29.0 35.0 2.0 35.0 94-95 27.742441211169563 33.0 27.0 35.0 2.0 35.0 96-97 27.09475644777335 33.0 26.5 35.0 2.0 35.0 98-99 26.318535537800766 33.0 25.0 34.0 2.0 35.0 100-101 23.26782033142584 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32528535138319015 1101 2 0.28357224042587603 1101 3 0.3009088601667429 1101 4 0.26450975395681553 1101 5 0.2640777863043908 1101 6 0.3133850633722375 1101 7 0.2679921564411174 1101 8 0.3148418876490595 1101 9 0.4360738578955434 1101 10-11 0.3443074719408017 1101 12-13 0.3487958233123791 1101 14-15 0.4837006936058472 1101 16-17 0.414645742199248 1101 18-19 0.45191510049230743 1101 20-21 0.3665595327253186 1101 22-23 0.37064607427915064 1101 24-25 0.4511801893004588 1101 26-27 0.3724828182985931 1101 28-29 0.4744903236989444 1101 30-31 0.42913716830864956 1101 32-33 0.5781887434755433 1101 34-35 0.5682068172332535 1101 36-37 0.6363177484864053 1101 38-39 0.6654333537484405 1101 40-41 0.8164651220852335 1101 42-43 0.6330392191289107 1101 44-45 0.6859275375999658 1101 46-47 0.8640595479803466 1101 48-49 0.9470508206283696 1101 50-51 0.6720129163797495 1101 52-53 0.6593873032978905 1101 54-55 0.7429202059944302 1101 56-57 0.747800040104373 1101 58-59 0.7891168889817735 1101 60-61 0.7210608383788255 1101 62-63 0.6536200182830072 1101 64-65 0.8189658955143386 1101 66-67 0.6860765395868498 1101 68-69 0.6668611595563547 1101 70-71 0.7006572429076918 1101 72-73 0.8092756661217244 1101 74-75 0.7235215244446458 1101 76-77 0.5466307093563785 1101 78-79 0.9311371805289816 1101 80-81 1.1320488762570058 1101 82-83 1.0195981558214626 1101 84-85 0.8904674897374321 1101 86-87 0.6775457911172005 1101 88-89 0.5197682238500896 1101 90-91 0.5019592658708163 1101 92-93 0.58188413864411 1101 94-95 0.808907182626136 1101 96-97 1.1056218258205135 1101 98-99 1.2351501168378576 1101 100-101 1.4709719066730003 1102 1 0.30784305207501816 1102 2 0.26652900053363027 1102 3 0.2434647212668395 1102 4 0.1440092777665356 1102 5 0.22087419093372773 1102 6 0.27362822770098916 1102 7 0.24429916367698468 1102 8 0.28866090894501184 1102 9 0.3087800911829959 1102 10-11 0.3196101280885202 1102 12-13 0.33493711880567645 1102 14-15 0.4853527170948695 1102 16-17 0.5285292564463901 1102 18-19 0.4778682956692748 1102 20-21 0.3160910967783721 1102 22-23 0.4331752819224519 1102 24-25 0.3400490507457903 1102 26-27 0.38951729331995466 1102 28-29 0.421950387853677 1102 30-31 0.5287662183492543 1102 32-33 0.5166884285588864 1102 34-35 0.6081478183093765 1102 36-37 0.6054229191002918 1102 38-39 0.5770572636562861 1102 40-41 0.627141126673493 1102 42-43 0.6442772272387955 1102 44-45 0.8197068963083893 1102 46-47 1.0112732883281623 1102 48-49 1.0435542145926107 1102 50-51 1.0075693990638754 1102 52-53 1.0109616390959673 1102 54-55 0.9494126242779899 1102 56-57 1.0803280021048423 1102 58-59 0.9112210271969801 1102 60-61 1.1111941097874904 1102 62-63 1.1505111701355304 1102 64-65 1.245752310253181 1102 66-67 1.268545209813638 1102 68-69 1.0574153510001096 1102 70-71 0.9170192151644976 1102 72-73 1.0988032746401082 1102 74-75 1.2717320265335736 1102 76-77 1.2725079285701355 1102 78-79 1.1842163312315535 1102 80-81 1.2662388433877076 1102 82-83 1.270994348196563 1102 84-85 1.1241476094841332 1102 86-87 1.139575133017697 1102 88-89 1.0828957112688897 1102 90-91 1.038559098162743 1102 92-93 1.0868736187367212 1102 94-95 1.3466681025209937 1102 96-97 1.3400675828186976 1102 98-99 1.2532055446439898 1102 100-101 1.2561651318608895 1103 1 0.1695243611260615 1103 2 0.17887476573932304 1103 3 0.2261981353929272 1103 4 0.09125397040151029 1103 5 0.18431216929474914 1103 6 0.25503721028539417 1103 7 0.18064569165520794 1103 8 0.14322092361692285 1103 9 0.22693001349899333 1103 10-11 0.20895053825590537 1103 12-13 0.1882980071230591 1103 14-15 0.3077921407639579 1103 16-17 0.21372954154825408 1103 18-19 0.3805191907286556 1103 20-21 0.3161093324604991 1103 22-23 0.10339573568874272 1103 24-25 0.19036536167771345 1103 26-27 0.23195266345740606 1103 28-29 0.25860895440401777 1103 30-31 0.518700121816849 1103 32-33 0.5546832537933213 1103 34-35 0.6062223907868329 1103 36-37 0.5851899087643062 1103 38-39 0.48057904279195895 1103 40-41 0.5968490535682065 1103 42-43 0.5369451283743061 1103 44-45 0.6362707831971406 1103 46-47 0.6207751728556516 1103 48-49 0.6788442192505144 1103 50-51 0.7787725476888525 1103 52-53 0.8549011924989003 1103 54-55 0.8951156202442618 1103 56-57 0.7887595041936066 1103 58-59 0.9831123383633624 1103 60-61 0.6941664634513529 1103 62-63 0.8899026923148057 1103 64-65 0.7301621045859505 1103 66-67 0.8447372763540812 1103 68-69 1.0547907153229872 1103 70-71 0.9443373090251193 1103 72-73 0.9690449338996139 1103 74-75 0.9625749527040703 1103 76-77 0.9739199955899096 1103 78-79 1.0852630072835154 1103 80-81 1.4056673058487767 1103 82-83 1.227838824921058 1103 84-85 1.118544305386937 1103 86-87 1.0712223257125864 1103 88-89 1.109902756494428 1103 90-91 1.0485996647401805 1103 92-93 0.8575148478425341 1103 94-95 1.0537642316587963 1103 96-97 1.444836786815543 1103 98-99 1.6022036762681005 1103 100-101 1.6753412020609701 1104 1 0.20866330870897443 1104 2 0.18381613816066533 1104 3 0.16368789162446262 1104 4 0.09738398910977253 1104 5 0.003061329490307685 1104 6 0.1526194594404231 1104 7 0.09877897065042873 1104 8 0.17925683993031782 1104 9 0.286887990053863 1104 10-11 0.2256189029886997 1104 12-13 0.3044523746229615 1104 14-15 0.18156528798923688 1104 16-17 0.2394810834661243 1104 18-19 0.22440009870676647 1104 20-21 0.1759775329976847 1104 22-23 0.1635881191977191 1104 24-25 0.17270879628961922 1104 26-27 0.2868374828939153 1104 28-29 0.12303941564307053 1104 30-31 0.26994450897002054 1104 32-33 0.24870207427228763 1104 34-35 0.33591278165212657 1104 36-37 0.496367761256991 1104 38-39 0.5623243151543846 1104 40-41 0.40052713862212386 1104 42-43 0.3167698141213222 1104 44-45 0.39418598620463996 1104 46-47 0.4451537913976509 1104 48-49 0.5654765259183065 1104 50-51 0.5324732244051162 1104 52-53 0.6418315466859639 1104 54-55 0.5597542053269677 1104 56-57 0.5549823111412095 1104 58-59 0.7006224270910124 1104 60-61 0.5896778102245079 1104 62-63 0.6027375620288353 1104 64-65 0.6914244174110777 1104 66-67 0.5548153726300029 1104 68-69 0.5898196045957889 1104 70-71 0.6642106628238444 1104 72-73 0.6363173671988314 1104 74-75 0.7450238889025549 1104 76-77 0.8569490797711694 1104 78-79 0.9763446163414464 1104 80-81 1.0666085365185083 1104 82-83 0.9551796090732303 1104 84-85 0.6640011663861998 1104 86-87 0.5661351410914541 1104 88-89 0.772581551782956 1104 90-91 0.9152819093178124 1104 92-93 0.9340485337829882 1104 94-95 1.1783561420717845 1104 96-97 1.3375681040275822 1104 98-99 1.545105095408406 1104 100-101 1.784048811316758 1105 1 0.21442026700099248 1105 2 0.20226019759716962 1105 3 0.06809695892457768 1105 4 0.10582752793806094 1105 5 0.15040616705448429 1105 6 0.21380666410119886 1105 7 0.17830187559584232 1105 8 0.2280438833236218 1105 9 0.1053028712159616 1105 10-11 0.14274200988167252 1105 12-13 0.19724764120463334 1105 14-15 0.3397248531966284 1105 16-17 0.3093179514870954 1105 18-19 0.3964874470796218 1105 20-21 0.2691624687752068 1105 22-23 0.0055114880898017304 1105 24-25 0.22450840571238473 1105 26-27 0.33930813209235566 1105 28-29 0.3084958013871244 1105 30-31 0.36342183285007934 1105 32-33 0.4243050414969787 1105 34-35 0.4607164941940596 1105 36-37 0.4331831765873275 1105 38-39 0.5997236591880295 1105 40-41 0.42251193928365893 1105 42-43 0.43743317795261305 1105 44-45 0.5540593210150462 1105 46-47 0.6030333783495152 1105 48-49 0.7001972196105939 1105 50-51 0.7124647372804773 1105 52-53 0.6354610576653528 1105 54-55 0.5653080658790302 1105 56-57 0.6681964373003169 1105 58-59 0.6321227957542774 1105 60-61 0.9442773838100607 1105 62-63 0.7192321253051333 1105 64-65 0.8013002947267225 1105 66-67 0.6066261811829889 1105 68-69 0.7678915260154184 1105 70-71 0.7071454039786538 1105 72-73 0.954432898636913 1105 74-75 0.7074175122994113 1105 76-77 0.5216492397068571 1105 78-79 0.6602140550652535 1105 80-81 0.869751436209409 1105 82-83 0.782308321584896 1105 84-85 0.4127580629316583 1105 86-87 0.4673721304262486 1105 88-89 0.45383947441089134 1105 90-91 0.6148815858142882 1105 92-93 0.6621539315216154 1105 94-95 0.6733855443288022 1105 96-97 0.6573238540242556 1105 98-99 0.37232491435781867 1105 100-101 0.6903387271356252 1106 1 0.03129348286963918 1106 2 0.0705001226995492 1106 3 0.0651054576600103 1106 4 0.10810202617953735 1106 5 -0.03143307493260039 1106 6 0.03069806829753219 1106 7 0.057775486246924856 1106 8 0.15335590887151795 1106 9 0.22155021715603596 1106 10-11 0.08579196268774325 1106 12-13 -0.19625060601494226 1106 14-15 0.07457014711042831 1106 16-17 0.11997363112747195 1106 18-19 0.123138899369728 1106 20-21 0.2571825831510708 1106 22-23 0.423124107343007 1106 24-25 0.12137484403576337 1106 26-27 0.18870064194756253 1106 28-29 0.0771172984588091 1106 30-31 0.019693072509049614 1106 32-33 0.13628617158564538 1106 34-35 0.26332575339305464 1106 36-37 0.3125691960813839 1106 38-39 0.3624033167879759 1106 40-41 0.4595113055640354 1106 42-43 0.4373068169391985 1106 44-45 0.532522905111172 1106 46-47 0.8022166267082511 1106 48-49 0.5029127369344977 1106 50-51 0.2987027784550307 1106 52-53 0.3685736740467149 1106 54-55 0.5347042899822014 1106 56-57 0.4813166353424876 1106 58-59 0.518224615927231 1106 60-61 0.49502760446318916 1106 62-63 0.5524547332049892 1106 64-65 0.5130339678307791 1106 66-67 0.5476739820227152 1106 68-69 0.6316331904963022 1106 70-71 0.6650274593745529 1106 72-73 0.6904513396866285 1106 74-75 0.8259336127985399 1106 76-77 0.6306748622741694 1106 78-79 0.7254809544148735 1106 80-81 1.212897691424196 1106 82-83 0.9515732068165512 1106 84-85 1.0596029624459362 1106 86-87 0.9722786711403444 1106 88-89 0.7997991767349752 1106 90-91 1.0017961370508033 1106 92-93 1.1122887166025883 1106 94-95 1.2849168483553157 1106 96-97 1.4557253872045521 1106 98-99 1.4347802811589538 1106 100-101 1.241317259165271 1107 1 -0.05009818065796878 1107 2 0.015638912365339763 1107 3 -0.0954580140949588 1107 4 0.01814657915601714 1107 5 -0.0107620525521952 1107 6 -0.02956924956132667 1107 7 -6.996509902990056E-6 1107 8 -0.03618388356097313 1107 9 -0.18209585295969788 1107 10-11 -0.07978469311128578 1107 12-13 0.10274467890328509 1107 14-15 0.0463920131397586 1107 16-17 0.03393702754504346 1107 18-19 0.050299329052656105 1107 20-21 0.11554243611325887 1107 22-23 0.026487012768598106 1107 24-25 0.12693162634965915 1107 26-27 -0.051762683029380696 1107 28-29 0.09722848606190837 1107 30-31 0.06066882166150833 1107 32-33 0.02360740763847957 1107 34-35 -0.16117123960577118 1107 36-37 -0.13093360309250102 1107 38-39 -0.05364744502895036 1107 40-41 -0.024885410764696303 1107 42-43 -0.12369698889815339 1107 44-45 -0.40031965272326886 1107 46-47 -0.3410010593945927 1107 48-49 -0.22370149663739625 1107 50-51 -0.32292749678624943 1107 52-53 -0.1684150383332934 1107 54-55 -0.16157884872952621 1107 56-57 -0.19147292653159553 1107 58-59 -0.1924888811566845 1107 60-61 -0.2678466470338208 1107 62-63 -0.13942831198445305 1107 64-65 -0.12262854661971545 1107 66-67 -0.18655127678721328 1107 68-69 0.060613407935605323 1107 70-71 -0.15210855060115946 1107 72-73 0.007428231889207382 1107 74-75 -0.31401728800289064 1107 76-77 -0.1090773036759245 1107 78-79 0.134306470411083 1107 80-81 0.3414317296384404 1107 82-83 0.37443615770436267 1107 84-85 0.2992836877631966 1107 86-87 0.2624971495847568 1107 88-89 0.35685621233838916 1107 90-91 0.20131683535051792 1107 92-93 0.002209742230295575 1107 94-95 0.06732881401185153 1107 96-97 0.07279969845190237 1107 98-99 0.3756423747591704 1107 100-101 0.567687295664939 1108 1 -0.049360805478453784 1108 2 -0.07749207670840619 1108 3 -0.056797047108879894 1108 4 -0.09171056490089313 1108 5 -0.11925362480740631 1108 6 -0.25569987750600376 1108 7 -0.12206862360633863 1108 8 -0.18108230551089832 1108 9 -0.1256841322216502 1108 10-11 -0.19307844221895465 1108 12-13 -0.15123823885166843 1108 14-15 -0.32917970457487655 1108 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pass #Quality Count 3 2.0 4 8.0 5 20.0 6 42.0 7 124.0 8 324.0 9 586.0 10 974.0 11 1557.0 12 2248.0 13 3164.0 14 3972.0 15 5660.0 16 7676.0 17 10458.0 18 9309.0 19 5742.0 20 5060.0 21 5293.0 22 5579.0 23 6186.0 24 7032.0 25 8408.0 26 10065.0 27 12558.0 28 15804.0 29 19271.0 30 24168.0 31 29758.0 32 37173.0 33 48241.0 34 64721.0 35 90311.0 36 137313.0 37 206593.0 38 154974.0 39 12357.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.120188012275186 20.27882678156539 15.571905666300259 22.02907953985917 2 15.255818933393236 21.4548435653209 40.30363212290561 22.98570537838025 3 18.948912035992194 25.970895952452683 30.46257233761367 24.617619673941455 4 12.870173081565605 18.066503626420914 37.712888226465004 31.350435065548478 5 13.38208018037593 38.094548309657945 34.25127299179051 14.272098518175625 6 30.490001385489307 37.635662494804414 17.686400792667822 14.187935327038453 7 27.105313981266505 33.76920267189514 21.491668171112128 17.633815175726227 8 25.83887792042035 35.3938309974169 20.065789818952044 18.70150126321071 9 26.274942822145647 17.144644489671247 20.87781520243427 35.70259748574883 10-11 24.30825247813656 27.58939554880071 27.58105112455549 20.52130084850724 12-13 25.494971823106415 24.964444318490738 28.277971431600314 21.26261242680253 14-15 22.63632375106536 26.147069690112225 26.879174836155396 24.337431722667024 16-17 21.335496936964926 29.395795142702852 27.414806794988934 21.853901125343288 18-19 21.388223673886678 27.69771580650562 30.19755967122897 20.71650084837874 20-21 22.336998620793892 26.76541530477936 30.359328324526984 20.53825774989976 22-23 21.897448600445877 26.662587170369 30.324110033041823 21.115854196143303 24-25 21.768815305317528 26.811544382030434 30.42379539587179 20.99584491678025 26-27 21.63223747743209 27.033841373808627 30.307186043582313 21.026735105176975 28-29 21.417939666474574 27.19212613798468 30.2976578483023 21.092276347238446 30-31 21.68069935436863 26.977196860611553 30.24860928619386 21.093494498825958 32-33 21.558660205924355 27.059335372039357 30.10196504040763 21.28003938162866 34-35 21.707967467386304 27.056157437949086 30.099437864061873 21.13643723060274 36-37 21.855513611340893 26.853424565499463 30.10467274414542 21.186389079014223 38-39 21.762275873687834 27.109134680737274 29.99307736192381 21.135512083651083 40-41 21.75352945406107 27.080395292556698 29.910571593372882 21.255503660009353 42-43 21.580550941197775 27.14648989521253 29.753239016735026 21.51972014685467 44-45 21.83685208238428 27.26124323768298 29.518445354600477 21.383459325332264 46-47 22.07371673255734 27.521413812847396 28.979720577360794 21.42514887723447 48-49 22.23054929275881 27.610536613219473 28.587952758219657 21.570961335802064 50-51 22.049817387329373 27.866341905291225 28.546619369486393 21.53722133789301 52-53 22.246795566511512 27.87540929575676 28.373728027660235 21.504067110071496 54-55 22.112109548435992 27.83084272145488 28.335507829761596 21.721539900347533 56-57 22.14627029381588 28.12734033257323 28.196300601696716 21.530088771914173 58-59 22.188990130436107 27.84937343016415 28.29010828915314 21.67152815024661 60-61 22.16532149928915 27.743860678480768 28.60842745306521 21.482390369164868 62-63 21.867347656368903 27.63728691519432 28.88034502918452 21.615020399252256 64-65 21.88032725623735 27.765502053224278 28.664679923749382 21.689490766788992 66-67 21.97154374476174 27.905204594607785 28.298129944039495 21.82512171659098 68-69 21.995375360939118 27.693988703956972 28.552003048105274 21.758632886998637 70-71 22.003410323655352 27.60619736316588 28.466440746282522 21.923951566896243 72-73 21.922721830817736 27.7070637035513 28.25156003394835 22.11865443168261 74-75 21.94554746962001 27.934318131004755 28.013515513554875 22.10661888582036 76-77 22.005351157930537 27.926945882767868 28.07332073043621 21.99438222886539 78-79 21.980533829085257 27.90100427554541 27.87822516052978 22.240236734839556 80-81 22.131896898278526 27.9763185543843 27.774949644652363 22.116834902684815 82-83 22.046374195038236 27.983068905252 27.879519463411306 22.09103743629846 84-85 21.989813456628468 27.998037214208683 27.70398671183523 22.30816261732762 86-87 21.9028541079758 27.846235877455573 27.804671198196345 22.446238816372286 88-89 21.896197476180106 27.78988635200701 27.74029163791135 22.57362453390153 90-91 21.91117907981938 27.87249429531681 27.84887785155438 22.36744877330943 92-93 21.71953917785904 27.969303014908704 27.77606924087781 22.535088566354442 94-95 21.822853580147914 27.93888274278229 27.632761150635126 22.605502526434673 96-97 22.02218886777996 28.03102662874057 27.347779538799028 22.599004964680443 98-99 21.727666810114094 28.014442706747978 27.266119467215265 22.991771015922666 100-101 21.72686351677799 28.06922064842664 27.201181037690446 23.002734797104925 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 254.0 1 172.0 2 125.5 3 154.5 4 136.0 5 152.5 6 258.5 7 524.5 8 889.0 9 1129.5 10 1087.5 11 993.0 12 1080.5 13 1215.0 14 1327.5 15 1459.0 16 1559.5 17 1589.5 18 1708.5 19 1883.5 20 2086.0 21 2329.0 22 2716.5 23 3328.0 24 4012.5 25 4897.0 26 6258.5 27 8037.0 28 9412.0 29 10877.0 30 12761.5 31 14739.5 32 16617.5 33 18482.0 34 21099.0 35 23251.5 36 25752.0 37 27937.0 38 29223.0 39 30195.5 40 30644.0 41 31406.5 42 31877.0 43 32826.5 44 34506.5 45 35428.0 46 35258.5 47 35205.5 48 35969.5 49 35419.5 50 33732.5 51 32665.5 52 31480.5 53 30534.0 54 29476.0 55 27133.5 56 24199.5 57 21514.0 58 19494.0 59 17521.5 60 14491.0 61 11321.0 62 8645.5 63 6273.5 64 4447.5 65 3179.5 66 2183.0 67 1551.5 68 1096.5 69 658.0 70 402.5 71 242.5 72 139.5 73 89.0 74 53.5 75 33.5 76 19.5 77 9.5 78 7.0 79 3.5 80 2.5 81 1.5 82 1.0 83 1.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025295676013821305 2 0.006927446543204175 3 0.008501866212114215 4 2.0992262252133865E-4 5 0.005772872119336813 6 0.0 7 0.0 8 1.0496131126066933E-4 9 3.1488393378200795E-4 10-11 0.0 12-13 1.0496131126066933E-4 14-15 0.0 16-17 5.248065563033466E-5 18-19 0.003411242615971753 20-21 0.001049613112606693 22-23 0.0 24-25 0.004565817039839115 26-27 0.005457988185554805 28-29 0.006402639986900828 30-31 0.02660769240457967 32-33 3.6736458941234257E-4 34-35 0.0027814747484077368 36-37 0.015114428821536382 38-39 0.006350159331270495 40-41 0.007399772443877187 42-43 0.009394037357829904 44-45 0.0027289940927774023 46-47 0.001836822947061713 48-49 0.0016793809801707092 50-51 0.003411242615971753 52-53 0.0029913973709290755 54-55 0.0022041875364740555 56-57 9.971324569763584E-4 58-59 5.248065563033465E-4 60-61 5.248065563033466E-5 62-63 1.0496131126066933E-4 64-65 0.008816750145896222 66-67 6.822485231943506E-4 68-69 9.446518013460238E-4 70-71 0.00383108786101443 72-73 0.01138830227178262 74-75 0.005143104251772797 76-77 0.004303413761687442 78-79 0.011020937682370278 80-81 3.1488393378200795E-4 82-83 0.004933181629251458 84-85 4.723259006730119E-4 86-87 0.0 88-89 9.971324569763584E-4 90-91 6.297678675640159E-4 92-93 0.0 94-95 6.297678675640159E-4 96-97 2.0992262252133865E-4 98-99 2.0992262252133865E-4 100-101 0.0011020937682370278 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 952732.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.20151562503062 #Duplication Level Percentage of deduplicated Percentage of total 1 82.0150673861253 53.47506697664388 2 10.800655251344962 14.08438184262275 3 3.103122489684662 6.069848684926753 4 1.3515770757347114 3.5249949528779996 5 0.6933890853012464 2.2605009639747458 6 0.4321811403370777 1.6907319224718913 7 0.3061907167501447 1.3974869161696721 8 0.21676381971969186 1.1306663662715848 9 0.15375082330467307 0.9022308037254869 >10 0.8587805541802647 10.040253938838068 >50 0.04986742243856047 2.249125043400839 >100 0.017689361055036306 1.8210712195585799 >500 4.8243701183491364E-4 0.2171486602014232 >1k 4.8243701183491364E-4 1.1364917083163817 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4598 0.4826121091765575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3807 0.39958771196936815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2434 0.25547583160846915 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0496131126066931E-4 2 0.0 0.0 0.0 0.0 1.0496131126066931E-4 3 0.0 0.0 0.0 0.0 1.0496131126066931E-4 4 0.0 0.0 0.0 0.0 1.0496131126066931E-4 5 0.0 0.0 0.0 0.0 1.0496131126066931E-4 6 0.0 0.0 0.0 0.0 1.0496131126066931E-4 7 0.0 0.0 0.0 0.0 1.0496131126066931E-4 8 0.0 0.0 0.0 0.0 1.0496131126066931E-4 9 0.0 0.0 0.0 0.0 1.0496131126066931E-4 10-11 0.0 0.0 0.0 0.0 1.0496131126066931E-4 12-13 0.0 0.0 0.0 0.0 1.0496131126066931E-4 14-15 0.0 0.0 0.0 0.0 1.0496131126066931E-4 16-17 0.0 0.0 0.0 1.0496131126066931E-4 1.0496131126066931E-4 18-19 1.0496131126066931E-4 0.0 0.0 1.0496131126066931E-4 1.0496131126066931E-4 20-21 2.0992262252133862E-4 0.0 0.0 2.0992262252133862E-4 1.0496131126066931E-4 22-23 2.0992262252133862E-4 0.0 0.0 2.6240327815167327E-4 1.0496131126066931E-4 24-25 2.0992262252133862E-4 0.0 0.0 4.7232590067301187E-4 1.0496131126066931E-4 26-27 2.0992262252133862E-4 0.0 0.0 8.921711457156892E-4 1.0496131126066931E-4 28-29 2.0992262252133862E-4 0.0 0.0 0.0020467455695830517 1.0496131126066931E-4 30-31 2.0992262252133862E-4 0.0 0.0 0.004670778351099784 1.0496131126066931E-4 32-33 2.0992262252133862E-4 0.0 0.0 0.007977059655810869 1.0496131126066931E-4 34-35 2.6240327815167327E-4 0.0 0.0 0.01102093768237028 1.0496131126066931E-4 36-37 3.1488393378200795E-4 0.0 0.0 0.01354000915262634 1.0496131126066931E-4 38-39 6.297678675640159E-4 0.0 0.0 0.017318616358010436 1.0496131126066931E-4 40-41 6.297678675640159E-4 0.0 0.0 0.023406372411129257 1.0496131126066931E-4 42-43 7.347291788246852E-4 0.0 0.0 0.033850022881565855 1.0496131126066931E-4 44-45 8.396904900853545E-4 0.0 0.0 0.049069413014362906 1.0496131126066931E-4 46-47 9.971324569763584E-4 0.0 0.0 0.0649710516703543 1.0496131126066931E-4 48-49 0.0011545744238673624 0.0 0.0 0.09761401947242246 1.0496131126066931E-4 50-51 0.0011545744238673624 0.0 0.0 0.13104419710894566 1.0496131126066931E-4 52-53 0.0011545744238673624 0.0 0.0 0.1673083301495069 3.1488393378200795E-4 54-55 0.0012595357351280318 0.0 0.0 0.236950160170961 3.1488393378200795E-4 56-57 0.0012595357351280318 0.0 0.0 0.30837633248384644 3.1488393378200795E-4 58-59 0.0012595357351280318 0.0 0.0 0.4053605840887049 3.1488393378200795E-4 60-61 0.0012595357351280318 0.0 0.0 0.5117388730513932 3.1488393378200795E-4 62-63 0.0012595357351280318 0.0 0.0 0.6520721462069081 3.1488393378200795E-4 64-65 0.0012595357351280318 0.0 0.0 0.7614418325405256 3.1488393378200795E-4 66-67 0.0012595357351280318 0.0 0.0 0.9021424702854528 3.1488393378200795E-4 68-69 0.0012595357351280318 0.0 0.0 1.089236007607596 3.1488393378200795E-4 70-71 0.0013644970463887011 0.0 0.0 1.2192830722595651 3.1488393378200795E-4 72-73 0.0014694583576493705 0.0 0.0 1.3945159814092527 3.1488393378200795E-4 74-75 0.0014694583576493705 0.0 0.0 1.6137276799771605 3.1488393378200795E-4 76-77 0.0014694583576493705 0.0 0.0 1.787910976014241 3.1488393378200795E-4 78-79 0.0014694583576493705 0.0 0.0 1.9471372851966766 3.1488393378200795E-4 80-81 0.0014694583576493705 0.0 0.0 2.170232552281229 3.1488393378200795E-4 82-83 0.001679380980170709 0.0 0.0 2.4915716067057683 3.1488393378200795E-4 84-85 0.001679380980170709 0.0 0.0 2.8738407023171257 3.1488393378200795E-4 86-87 0.001679380980170709 0.0 0.0 3.280250899518437 3.1488393378200795E-4 88-89 0.001679380980170709 0.0 0.0 3.68991489736883 3.1488393378200795E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5140 0.0 36.701817 1 GGTATCA 3080 0.0 36.255787 1 ATCCCGT 60 0.0039543677 31.660526 6 AATACCG 65 0.0058446173 29.229704 5 TATCAAC 6505 0.0 28.99427 2 ATCAACG 6470 0.0 28.927792 3 TCAACGC 6500 0.0 28.791258 4 CAACGCA 6610 0.0 28.31213 5 AACGCAG 6805 0.0 27.566294 6 GGACCGA 135 1.3444336E-5 24.624851 6 ACGCAGA 7565 0.0 24.545801 7 CGCAGAG 7635 0.0 23.94755 8 GAGTACT 4230 0.0 22.119202 12-13 GCAGAGT 8150 0.0 22.026403 9 AGTACTT 4390 0.0 21.258942 12-13 GTACATG 4255 0.0 21.1068 1 GTACTTT 4610 0.0 20.504667 14-15 TACATGG 4200 0.0 20.47379 2 AGAGTAC 6925 0.0 20.470758 10-11 ACATGGG 4150 0.0 20.031494 3 >>END_MODULE