FastQCFastQC Report
Thu 26 May 2016
SRR937133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048449
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45000.4292054263011363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33610.32056876395513756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19870.18951804045785728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27650.040.392251
GTATCAA48400.039.5717241
TCAACGC58800.032.1685144
ATCAACG59000.032.059473
CAACGCA60000.031.5251485
TATCAAC60850.031.1628822
AACGCAG62150.030.5831326
CGCAGAG68850.027.263218
ACGCAGA69600.027.1059787
ACAGCGT1457.856361E-726.2183788
GTACATG43950.025.0873281
TACATGG43500.025.019092
GCAGAGT72500.024.7740049
GTATAGG4000.023.7626741
GAGTACT37000.023.30532312-13
ACATGGG43750.023.2466833
GTACTTT39900.021.7869414-15
CATGGGA25500.020.1283844
AGTACTT40650.019.8686612-13
AGAGTAC62450.019.8582110-11
Produced by FastQC (version 0.11.5)