##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937133_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1048449 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.652050791216357 33.0 31.0 34.0 30.0 34.0 2 31.76685847380273 33.0 31.0 34.0 30.0 34.0 3 31.754893180307292 33.0 31.0 34.0 30.0 34.0 4 35.34977571632001 37.0 35.0 37.0 33.0 37.0 5 35.343569405855696 37.0 35.0 37.0 33.0 37.0 6 35.426171420832105 37.0 35.0 37.0 33.0 37.0 7 35.35020301416664 37.0 35.0 37.0 33.0 37.0 8 35.309637378642165 37.0 35.0 37.0 33.0 37.0 9 36.91166380052821 39.0 37.0 39.0 33.0 39.0 10-11 36.784372439670406 39.0 37.0 39.0 32.5 39.0 12-13 36.69254060044885 39.0 37.0 39.0 32.0 39.0 14-15 37.82496955025948 40.0 37.5 41.0 32.5 41.0 16-17 37.74767060677247 40.0 37.0 41.0 32.0 41.0 18-19 37.715776351544044 40.0 37.0 41.0 32.0 41.0 20-21 37.62130489895074 40.0 37.0 41.0 32.0 41.0 22-23 37.472232793392905 40.0 37.0 41.0 32.0 41.0 24-25 37.58126384783618 40.0 37.0 41.0 32.0 41.0 26-27 37.53562071211857 40.0 37.0 41.0 32.0 41.0 28-29 37.43986688909045 40.0 37.0 41.0 32.0 41.0 30-31 37.246214169692564 40.0 37.0 41.0 31.0 41.0 32-33 37.031722096162994 39.5 36.0 41.0 31.0 41.0 34-35 36.833130652993134 39.0 36.0 41.0 30.0 41.0 36-37 36.65536664158199 39.0 35.5 41.0 30.0 41.0 38-39 36.51311842540744 39.0 35.0 41.0 30.0 41.0 40-41 36.31515600663456 39.0 35.0 41.0 30.0 41.0 42-43 36.08201543422713 39.0 35.0 40.0 29.5 41.0 44-45 35.80712938826781 39.0 35.0 40.0 28.5 41.0 46-47 35.46240780428995 38.5 35.0 40.0 26.5 41.0 48-49 35.21800774286589 38.5 35.0 40.0 26.0 41.0 50-51 34.52432927114242 38.0 34.0 39.5 25.0 40.5 52-53 34.71488121978275 38.0 34.5 39.5 26.0 40.5 54-55 35.12106645149168 39.0 35.0 40.0 26.0 41.0 56-57 35.03234444403114 39.0 35.0 40.5 26.0 41.0 58-59 34.79670160398837 38.0 34.0 40.0 25.0 41.0 60-61 34.53937482891395 38.0 34.0 40.0 24.5 41.0 62-63 34.202995090843714 38.0 34.0 40.0 23.0 41.0 64-65 33.85285121164692 37.0 33.5 40.0 22.5 41.0 66-67 33.48074250631171 37.0 33.0 39.5 22.0 41.0 68-69 33.08741340780524 36.0 33.0 39.0 21.0 41.0 70-71 32.69514301601699 36.0 32.0 39.0 20.0 40.5 72-73 32.28444349701321 35.0 32.0 38.5 18.5 40.0 74-75 31.84255028141569 35.0 31.5 37.5 18.0 39.5 76-77 31.39879908321721 35.0 31.0 37.0 14.5 39.0 78-79 30.980639497009392 35.0 31.0 36.5 12.0 39.0 80-81 30.5396395055935 35.0 30.5 36.0 7.5 38.5 82-83 30.115915509481148 34.0 30.0 35.5 7.0 37.0 84-85 29.71444581472251 34.0 30.0 35.0 2.0 37.0 86-87 29.35970037646085 34.0 29.0 35.0 2.0 36.0 88-89 28.995804755405366 34.0 29.0 35.0 2.0 36.0 90-91 28.57845064471424 34.0 29.0 35.0 2.0 36.0 92-93 28.21547590774563 34.0 29.0 35.0 2.0 35.0 94-95 27.79406819025055 34.0 27.5 35.0 2.0 35.0 96-97 27.25879704210696 34.0 26.5 35.0 2.0 35.0 98-99 26.596350418570673 33.5 25.0 35.0 2.0 35.0 100-101 24.156158764040978 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45492726234390446 1101 2 0.43414249629886115 1101 3 0.4737822468041415 1101 4 0.3974768227850589 1101 5 0.44178388704770555 1101 6 0.23914367012582716 1101 7 0.2625915661033602 1101 8 0.2633304204651168 1101 9 0.4560593686373693 1101 10-11 0.47213366990178685 1101 12-13 0.5663892358039391 1101 14-15 0.6650546411646516 1101 16-17 0.6253909788910974 1101 18-19 0.7485297202272605 1101 20-21 0.7307099204255891 1101 22-23 0.6998357004682987 1101 24-25 0.7447164849169496 1101 26-27 0.6449497309310814 1101 28-29 0.6904600930510014 1101 30-31 0.7528072291888108 1101 32-33 0.8305725425416668 1101 34-35 0.8017203685595504 1101 36-37 0.640269432884665 1101 38-39 0.8040607969028812 1101 40-41 0.7859451440600793 1101 42-43 0.7991503724987439 1101 44-45 0.8158946421495088 1101 46-47 0.7938772559533049 1101 48-49 1.0145201126980652 1101 50-51 1.1184300264326552 1101 52-53 1.1959843236992995 1101 54-55 1.2469825838870676 1101 56-57 1.0244712957025328 1101 58-59 0.9563342352944773 1101 60-61 0.9905768406874742 1101 62-63 1.2961206072051255 1101 64-65 1.2295667638103254 1101 66-67 1.2222562915642712 1101 68-69 1.2108656030740548 1101 70-71 0.979839915117509 1101 72-73 0.976926136521115 1101 74-75 1.0445904089476734 1101 76-77 1.150982554932913 1101 78-79 1.232684228311495 1101 80-81 1.1660568762192405 1101 82-83 0.9341518014079284 1101 84-85 0.8856812920404735 1101 86-87 1.0214713809016196 1101 88-89 1.1370605450896996 1101 90-91 1.0544185431046067 1101 92-93 1.0193820102773046 1101 94-95 1.0118006325836326 1101 96-97 0.8450755380733987 1101 98-99 0.6362897689406601 1101 100-101 1.0032442729697486 1102 1 0.21088841396500868 1102 2 0.27337887812855755 1102 3 0.2852121332196873 1102 4 0.2316922932142873 1102 5 0.2852586485386652 1102 6 0.21116372400431516 1102 7 0.2350398085267713 1102 8 0.28566262837240686 1102 9 0.3150445159903299 1102 10-11 0.36570849470513167 1102 12-13 0.2631796535396518 1102 14-15 0.3352226650974117 1102 16-17 0.6443337424474436 1102 18-19 0.5219973719565658 1102 20-21 0.3898060075379135 1102 22-23 0.4778102083663214 1102 24-25 0.1054206833399789 1102 26-27 0.34470083202823076 1102 28-29 0.27243845006636747 1102 30-31 0.2620115655709796 1102 32-33 0.4574325580892591 1102 34-35 0.21143526826153902 1102 36-37 0.23518389249125704 1102 38-39 0.2671106442319129 1102 40-41 0.37689571118092857 1102 42-43 0.4287183371262415 1102 44-45 0.19159488498049626 1102 46-47 0.20865600039915222 1102 48-49 0.3298140000203631 1102 50-51 0.48496056577849345 1102 52-53 0.5281925172130499 1102 54-55 0.5847546524842429 1102 56-57 0.6288422535282407 1102 58-59 0.5134152602450399 1102 60-61 0.30008274087374076 1102 62-63 0.4810846061655667 1102 64-65 0.6530546800777088 1102 66-67 0.6406643346395882 1102 68-69 0.6609126580184252 1102 70-71 0.5877451457726011 1102 72-73 0.48577040526323856 1102 74-75 0.6342223499812967 1102 76-77 0.7011115266965113 1102 78-79 0.5884714140251113 1102 80-81 0.5622532109710896 1102 82-83 0.7480646661839288 1102 84-85 0.6813283089745283 1102 86-87 0.4899537272832539 1102 88-89 0.6693398362201286 1102 90-91 0.5180033398925055 1102 92-93 0.36498082737782767 1102 94-95 0.5065965389179645 1102 96-97 0.38751715158858957 1102 98-99 0.233813430937321 1102 100-101 0.29039993324449753 1103 1 0.23947795970925156 1103 2 0.34466741120603217 1103 3 0.27551436186303135 1103 4 0.29274855211978235 1103 5 0.32590235353747943 1103 6 0.26852583591205814 1103 7 0.15248327208652768 1103 8 0.2846144205166752 1103 9 0.1745906715678558 1103 10-11 0.28791661295480253 1103 12-13 0.3284606337896179 1103 14-15 0.26243680776922673 1103 16-17 0.41403535908159483 1103 18-19 0.3810309779256116 1103 20-21 0.37808761680503267 1103 22-23 0.49130010734921825 1103 24-25 0.22041758909324471 1103 26-27 0.3704856822819309 1103 28-29 0.36737902157997837 1103 30-31 0.3719668595983663 1103 32-33 0.34586081477817743 1103 34-35 0.26763536226747675 1103 36-37 0.47763568671530265 1103 38-39 0.36619886977963034 1103 40-41 0.2924102966604778 1103 42-43 0.3597886183537611 1103 44-45 0.4871544907101395 1103 46-47 0.5020297599851986 1103 48-49 0.512719338254584 1103 50-51 0.44042259296996633 1103 52-53 0.5084419102646436 1103 54-55 0.5172402222947525 1103 56-57 0.6800098637206347 1103 58-59 0.6831395464538588 1103 60-61 0.5166039848764754 1103 62-63 0.6765045432823413 1103 64-65 0.5320796850114036 1103 66-67 0.6100147692899682 1103 68-69 0.5444195834463912 1103 70-71 0.6085794323372866 1103 72-73 0.32861225020150187 1103 74-75 0.452750093981944 1103 76-77 0.41355294052000957 1103 78-79 0.3499968476240518 1103 80-81 0.5067656718423912 1103 82-83 0.6067422835723448 1103 84-85 0.48707847778313607 1103 86-87 0.42141188701293686 1103 88-89 0.45648165809597074 1103 90-91 0.43908029027317497 1103 92-93 0.3885103900283511 1103 94-95 0.6075179727812703 1103 96-97 0.42168466056513765 1103 98-99 0.4400812563635732 1103 100-101 0.49954963701485866 1104 1 0.46781912391029934 1104 2 0.5111816317499844 1104 3 0.5064892870828643 1104 4 0.4442714284854077 1104 5 0.5299480557803165 1104 6 0.5119063547533713 1104 7 0.3244062583496472 1104 8 0.44198775885135433 1104 9 0.5733370719447564 1104 10-11 0.4953105028646476 1104 12-13 0.5696975629413572 1104 14-15 0.6949052919984098 1104 16-17 0.7676082511977498 1104 18-19 0.77460680642357 1104 20-21 0.8473833776671 1104 22-23 0.6985529084499262 1104 24-25 0.7869529652149438 1104 26-27 0.6741219636951072 1104 28-29 0.6298677042751137 1104 30-31 0.5379182736303676 1104 32-33 0.6700937392378918 1104 34-35 0.570300903114628 1104 36-37 0.6055070608026014 1104 38-39 0.42153774155854506 1104 40-41 0.44336153878405327 1104 42-43 0.6208005921629649 1104 44-45 0.43283793202571275 1104 46-47 0.5765429261574653 1104 48-49 0.6573911217007549 1104 50-51 0.4232057315718336 1104 52-53 0.37333597905217886 1104 54-55 0.5036540359268074 1104 56-57 0.5888373769986259 1104 58-59 0.5437091115998669 1104 60-61 0.49398517214731896 1104 62-63 0.7090590436746282 1104 64-65 0.7657589930908202 1104 66-67 0.6061965437132883 1104 68-69 0.603639868764823 1104 70-71 0.5851864121911632 1104 72-73 0.6270372282793062 1104 74-75 0.5849871677845577 1104 76-77 0.5388848709153713 1104 78-79 0.7319570869081957 1104 80-81 0.5568306718129641 1104 82-83 0.6326840397578728 1104 84-85 0.7260846240514987 1104 86-87 0.8514059739824944 1104 88-89 0.8251399019723884 1104 90-91 0.7048846374191697 1104 92-93 0.5155620241960648 1104 94-95 0.5750551568840443 1104 96-97 0.654471903287142 1104 98-99 0.8464712320269143 1104 100-101 0.8131663642314955 1105 1 0.18539114750915786 1105 2 0.29997521280058237 1105 3 0.2995784999882716 1105 4 0.36090727737528994 1105 5 0.48134448677787134 1105 6 0.34767978588698156 1105 7 0.22611960206518233 1105 8 0.41268087130031716 1105 9 0.41029895499116975 1105 10-11 0.4568992414645976 1105 12-13 0.5460835096611163 1105 14-15 0.3894357482994266 1105 16-17 0.5114445010382056 1105 18-19 0.4218635723476396 1105 20-21 0.29025540121919846 1105 22-23 0.3979884171676531 1105 24-25 0.14097698765247202 1105 26-27 -0.07505951459507543 1105 28-29 0.17276698461185447 1105 30-31 -0.151701987464385 1105 32-33 0.16699497476970748 1105 34-35 -0.22098459323703423 1105 36-37 0.03602844148263529 1105 38-39 0.26945353258737725 1105 40-41 -0.009535417946857194 1105 42-43 0.19582173604091224 1105 44-45 -0.1074707849558294 1105 46-47 -0.006151493495103466 1105 48-49 -0.016721057500205916 1105 50-51 0.07016538451768639 1105 52-53 -0.005558573658369426 1105 54-55 -0.09235787103357751 1105 56-57 0.03381069760900601 1105 58-59 0.1697189100165417 1105 60-61 0.15170190449364185 1105 62-63 0.18974903772301843 1105 64-65 0.15963566058189116 1105 66-67 0.21620408491286724 1105 68-69 0.2541302805012222 1105 70-71 0.2536901456226204 1105 72-73 0.22358281653728795 1105 74-75 0.18556306670910772 1105 76-77 0.028141349778270097 1105 78-79 0.06318569465688384 1105 80-81 0.3689762576398117 1105 82-83 0.18727077687763938 1105 84-85 0.3702138726385158 1105 86-87 0.18075629472892984 1105 88-89 0.26345003742879314 1105 90-91 0.13278132532244413 1105 92-93 0.045158778067300176 1105 94-95 0.12092439818700385 1105 96-97 0.1643937469117418 1105 98-99 -0.0277157981383489 1105 100-101 -0.10281448028000639 1106 1 0.09863690170298511 1106 2 0.2591497250703725 1106 3 0.3156200497893735 1106 4 0.24579005404038412 1106 5 0.30386003453901367 1106 6 0.30893205822610526 1106 7 0.18614938464291697 1106 8 0.35857405499172756 1106 9 0.38472995047315806 1106 10-11 0.34252151980479795 1106 12-13 0.35271782861416057 1106 14-15 0.5196754602481661 1106 16-17 0.439674699032949 1106 18-19 0.22207020079378026 1106 20-21 0.556101021890953 1106 22-23 0.39788243655333133 1106 24-25 0.5048968663080444 1106 26-27 0.373539549940368 1106 28-29 0.43613252390129276 1106 30-31 0.49278637930684965 1106 32-33 0.23044934876516265 1106 34-35 0.4627729663196831 1106 36-37 0.3060775098320292 1106 38-39 0.23768128695265744 1106 40-41 0.4494942991524056 1106 42-43 0.19281985201611462 1106 44-45 0.26796317865839825 1106 46-47 0.47966499263325346 1106 48-49 0.6279492314083441 1106 50-51 0.356969515466524 1106 52-53 0.5783098942737155 1106 54-55 0.3251048315530838 1106 56-57 0.5424950326616553 1106 58-59 0.6356144158309718 1106 60-61 0.5233644433166376 1106 62-63 0.6882457330854592 1106 64-65 0.656934504988179 1106 66-67 0.7921526957905272 1106 68-69 0.7152671217253896 1106 70-71 0.7493938805279754 1106 72-73 0.4232664947801368 1106 74-75 0.7414290262083121 1106 76-77 0.8458857447875978 1106 78-79 0.9692855202951485 1106 80-81 0.9591090574248788 1106 82-83 1.0101940462378352 1106 84-85 0.7842835079774488 1106 86-87 1.0110165277647738 1106 88-89 0.8836286788923253 1106 90-91 0.9572156622314374 1106 92-93 0.7020350635885251 1106 94-95 0.7881125299782887 1106 96-97 0.7937994521789768 1106 98-99 0.5669772057235285 1106 100-101 0.9301168017546608 1107 1 0.3131730992580515 1107 2 0.21199624961500874 1107 3 0.20007102857997694 1107 4 0.3145177623907287 1107 5 0.3429272566251029 1107 6 0.21312765298774394 1107 7 0.04219773880447519 1107 8 0.2504469619589713 1107 9 0.4321121280440181 1107 10-11 0.4451829045416531 1107 12-13 0.407916717775322 1107 14-15 0.3327114808313567 1107 16-17 0.46089771082199604 1107 18-19 0.4512691988607074 1107 20-21 0.5578612113869923 1107 22-23 0.3640331621380142 1107 24-25 0.2278623704056244 1107 26-27 0.22720695172098004 1107 28-29 0.41879336410930534 1107 30-31 0.4271025872079619 1107 32-33 0.6287572253749261 1107 34-35 0.5000146554481617 1107 36-37 0.5988216629535472 1107 38-39 0.5133136210755609 1107 40-41 0.4658465004102652 1107 42-43 0.4558517886329554 1107 44-45 0.5151479645224342 1107 46-47 0.7129819901011913 1107 48-49 0.809787833058266 1107 50-51 0.5097221087851622 1107 52-53 0.8891446569952706 1107 54-55 0.7231567796050413 1107 56-57 1.0347790589079509 1107 58-59 0.8541583456956516 1107 60-61 0.9052289100867696 1107 62-63 0.8975585376812774 1107 64-65 0.9052029032565727 1107 66-67 0.9212754598189505 1107 68-69 1.0579118983324278 1107 70-71 0.9836175185252003 1107 72-73 1.0717921829284691 1107 74-75 1.1258418879796643 1107 76-77 1.0548255471236239 1107 78-79 1.0146257146919453 1107 80-81 1.1036828274703474 1107 82-83 1.1455394136586747 1107 84-85 1.0812790700673531 1107 86-87 0.9275089452005894 1107 88-89 0.9794113029768319 1107 90-91 0.9006459216994323 1107 92-93 0.8028722766898895 1107 94-95 1.1366477473814278 1107 96-97 0.9585126216091275 1107 98-99 0.869859124643181 1107 100-101 0.6512676634715611 1108 1 -0.02681103772377824 1108 2 -0.07861890889853385 1108 3 -0.15650862476642047 1108 4 0.001587069783361983 1108 5 0.10505896633187461 1108 6 -0.26507469380582904 1108 7 -0.25448502416004004 1108 8 -0.21246856746925857 1108 9 -0.15111972862894874 1108 10-11 -0.3027047479649383 1108 12-13 -0.07319812581065577 1108 14-15 -0.004628430988923071 1108 16-17 0.02397653602552907 1108 18-19 0.006754770512550579 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-1.188204985012625 2316 74-75 -1.5137266950468629 2316 76-77 -1.7352056055243459 2316 78-79 -1.8204634951636187 2316 80-81 -1.8021134530922893 2316 82-83 -1.4762343992366347 2316 84-85 -1.6804220533160148 2316 86-87 -1.6519491875285723 2316 88-89 -1.3815110042071588 2316 90-91 -1.1173848533406847 2316 92-93 -1.8296255992659312 2316 94-95 -1.6861550469166922 2316 96-97 -1.7340909845458796 2316 98-99 -1.5357719524832198 2316 100-101 -1.8625669442882788 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2550.0 3 874.0 4 835.0 5 890.0 6 1061.0 7 1262.0 8 1523.0 9 1943.0 10 2421.0 11 3066.0 12 3770.0 13 4689.0 14 5240.0 15 6459.0 16 8090.0 17 10525.0 18 9408.0 19 6226.0 20 6102.0 21 6525.0 22 7062.0 23 7876.0 24 8930.0 25 10172.0 26 11863.0 27 13948.0 28 16725.0 29 20118.0 30 24756.0 31 30580.0 32 38192.0 33 49766.0 34 67006.0 35 93189.0 36 139376.0 37 205410.0 38 186108.0 39 33900.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01635365567456 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21.30993502696319 27.479893272891527 30.114826081937952 21.095345618207332 20-21 22.38098713107549 26.56695289184733 30.134202931315972 20.91785704576121 22-23 21.98853748210919 26.459606237662435 30.069531517154907 21.482324763073464 24-25 21.631493506493506 26.72104628626368 30.160658965006792 21.486801242236027 26-27 21.51270258515444 26.847206537663286 30.009213618605784 21.630877258576486 28-29 21.455802751746667 26.867873238429073 30.15981114528936 21.5165128645349 30-31 21.614499131574355 26.74469621316821 30.0425199508989 21.598284704358534 32-33 21.431681475098134 26.811217178193814 30.00914210609898 21.747959240609074 34-35 21.72487333658894 26.853501556586494 29.841203378708336 21.580421728116225 36-37 21.689991597111543 26.653847731267806 29.880900263150618 21.775260408470036 38-39 21.57167396792786 26.89200905337313 29.835452177454506 21.700864801244503 40-41 21.74489257221504 26.744947415156776 29.852873078649072 21.657286933979115 42-43 21.549767632618703 26.80658783582392 29.653988397130043 21.98965613442733 44-45 21.7132474444357 26.98494679037339 29.463897810810746 21.837907954380167 46-47 21.886970073861555 27.08536808692467 29.074164860822854 21.953496978390916 48-49 21.843104489228313 27.20157414375412 28.840337529078834 22.114983837938734 50-51 21.90535734213109 27.348969763908404 28.73053434167995 22.015138552280558 52-53 22.025222042485655 27.455868102185327 28.507899294957877 22.01101056037114 54-55 21.89982435616735 27.413266617609118 28.46226519023472 22.224643835988818 56-57 21.87161404285191 27.649869139909654 28.39559043462337 22.082926382615064 58-59 21.995568667061537 27.37597561336191 28.481328980211607 22.147126739364946 60-61 21.9362644573866 27.246367411720048 28.831123989675948 21.986244141217405 62-63 21.773243586356347 27.293836778646906 28.942112436798297 21.990807198198446 64-65 21.761808019533785 27.28949677616268 28.798166800198388 22.150528404105145 66-67 21.74079352269974 27.476634444234588 28.4695394042761 22.31303262878957 68-69 21.669808207051677 27.371179355882468 28.70283635309133 22.25617608397452 70-71 21.756858345449203 27.216250147870852 28.72634543393894 22.30054607274101 72-73 21.79089955165506 27.234427167795477 28.508346847276545 22.46632643327292 74-75 21.673761706816247 27.57993546494573 28.26821398164114 22.478088846596886 76-77 21.777275736858474 27.421849210907435 28.324950437239906 22.475924614994188 78-79 21.74639917082075 27.490697728771956 28.283865108600825 22.479037991806468 80-81 21.64113270867337 27.596760826864163 28.2061692765201 22.55593718794237 82-83 21.850695430031223 27.53048991381963 28.209485326915324 22.409329329233824 84-85 21.791696874880756 27.43241309573778 28.0404090510169 22.73548097836456 86-87 21.73221871871466 27.43027020994177 28.280205987802326 22.557305083541245 88-89 21.812355337943444 27.266743835661515 28.15108716233199 22.769813664063047 90-91 21.78140652822105 27.341904260835015 28.291931139388314 22.584758071555623 92-93 21.815036865163563 27.407350516722893 28.166883557984402 22.61072906012914 94-95 21.754407050708803 27.436343925707913 28.069339537377125 22.739909486206162 96-97 21.81513950228215 27.516671921483965 27.93160374321067 22.736584833023212 98-99 21.707871493415574 27.414674404348748 27.827986843078182 23.04946725915749 100-101 22.005488502484237 27.304059411703523 27.74146023555141 22.94899185026083 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 161.0 1 128.5 2 110.0 3 128.5 4 127.5 5 147.0 6 227.0 7 460.0 8 852.0 9 1084.5 10 1069.0 11 1010.0 12 1030.0 13 1159.5 14 1311.5 15 1369.0 16 1388.0 17 1482.5 18 1634.5 19 1773.5 20 1932.0 21 2241.5 22 2776.5 23 3443.0 24 4095.0 25 5071.0 26 6576.0 27 8370.5 28 9953.5 29 11643.0 30 13730.0 31 15846.0 32 17897.0 33 20224.5 34 23198.0 35 25636.0 36 28267.5 37 30793.5 38 32139.5 39 33291.0 40 33785.5 41 34524.5 42 35248.0 43 36225.5 44 38057.0 45 39102.0 46 39270.5 47 39288.0 48 39772.5 49 39362.0 50 37609.0 51 35780.5 52 34686.5 53 34099.5 54 32740.0 55 30084.5 56 26969.5 57 24134.0 58 22120.0 59 19746.0 60 16363.5 61 12964.5 62 9710.0 63 7048.5 64 5047.5 65 3622.5 66 2485.0 67 1706.5 68 1185.5 69 739.5 70 460.5 71 272.0 72 166.5 73 115.5 74 72.0 75 46.0 76 33.5 77 23.0 78 18.0 79 13.0 80 9.5 81 7.0 82 5.5 83 6.5 84 6.5 85 4.0 86 1.5 87 2.5 88 4.0 89 3.0 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023177093020261358 2 0.009633277345869946 3 0.013257678723523986 4 0.023940124889241153 5 0.058467316960577005 6 0.04826176571297221 7 0.018122006888270197 8 0.04959707148368686 9 0.03099816967730428 10-11 0.025800015069879414 12-13 0.037817767006311225 14-15 0.021412581823245576 16-17 0.02002958656071969 18-19 0.01602366924857575 20-21 0.014163779067937496 22-23 0.007201113263496841 24-25 0.0016214427215820704 26-27 9.537898362247471E-5 28-29 0.0022890956069393934 30-31 0.0 32-33 3.3382644267866153E-4 34-35 9.537898362247471E-5 36-37 0.0 38-39 0.0 40-41 4.7689491811237355E-5 42-43 1.4306847543371209E-4 44-45 1.4306847543371209E-4 46-47 9.537898362247471E-5 48-49 5.722739017348483E-4 50-51 0.0 52-53 9.537898362247471E-5 54-55 0.0019552691642607317 56-57 9.537898362247471E-5 58-59 5.722739017348483E-4 60-61 0.002193716623316919 62-63 0.0019075796724494945 64-65 8.584108526022725E-4 66-67 0.010587067182094694 68-69 0.012494646854544189 70-71 0.02260481911852651 72-73 0.014211468559748733 74-75 0.01731128552747916 76-77 0.026992252365160344 78-79 0.01750204349472411 80-81 0.04439891687626198 82-83 0.034002607661412235 84-85 0.01611904823219823 86-87 0.01282847329722285 88-89 0.009585587854058709 90-91 0.020554170970643302 92-93 0.02909059000485479 94-95 0.009585587854058709 96-97 0.01950500215079608 98-99 0.031618133070850374 100-101 0.041966752793888874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1048449.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.32544429083899 #Duplication Level Percentage of deduplicated Percentage of total 1 82.51220980022167 54.72658974418621 2 10.626393268540028 14.096005094901962 3 2.990522137543688 5.950431283025237 4 1.2372835515616865 3.2825352508430417 5 0.6650552672792828 2.2055043040130546 6 0.42121809790096965 1.6762486491974358 7 0.2900554063282466 1.3466637585576549 8 0.19986309804227056 1.0604807019997684 9 0.15179589537726298 0.906113718218041 >10 0.8440130642005902 9.992790042576905 >50 0.04318039091458738 1.9578526691592795 >100 0.0178347096711432 1.7784438272088028 >500 1.4382810462058144E-4 0.08089464573517996 >1k 4.314843138617443E-4 0.93944631037742 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4500 0.4292054263011363 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3361 0.32056876395513756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1987 0.18951804045785728 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 9.537898362247472E-5 0.0 0.0 0.0 9.537898362247472E-5 3 9.537898362247472E-5 0.0 0.0 0.0 9.537898362247472E-5 4 9.537898362247472E-5 0.0 0.0 0.0 9.537898362247472E-5 5 1.9075796724494945E-4 0.0 0.0 0.0 9.537898362247472E-5 6 1.9075796724494945E-4 0.0 0.0 0.0 9.537898362247472E-5 7 1.9075796724494945E-4 0.0 0.0 0.0 9.537898362247472E-5 8 1.9075796724494945E-4 0.0 0.0 0.0 9.537898362247472E-5 9 1.9075796724494945E-4 0.0 0.0 0.0 9.537898362247472E-5 10-11 1.9075796724494945E-4 0.0 0.0 0.0 9.537898362247472E-5 12-13 1.9075796724494945E-4 0.0 0.0 0.0 9.537898362247472E-5 14-15 4.768949181123736E-4 0.0 0.0 0.0 9.537898362247472E-5 16-17 4.768949181123736E-4 0.0 0.0 4.768949181123736E-5 1.9075796724494945E-4 18-19 4.768949181123736E-4 0.0 0.0 9.537898362247472E-5 1.9075796724494945E-4 20-21 4.768949181123736E-4 0.0 0.0 9.537898362247472E-5 1.9075796724494945E-4 22-23 4.768949181123736E-4 0.0 0.0 2.384474590561868E-4 1.9075796724494945E-4 24-25 6.676528853573231E-4 0.0 0.0 5.722739017348483E-4 1.9075796724494945E-4 26-27 6.676528853573231E-4 0.0 0.0 0.0010014793280359847 1.9075796724494945E-4 28-29 7.630318689797978E-4 0.0 0.0 0.0019075796724494945 1.9075796724494945E-4 30-31 7.630318689797978E-4 9.537898362247472E-5 0.0 0.003385953918597853 1.9075796724494945E-4 32-33 7.630318689797978E-4 9.537898362247472E-5 0.0 0.005722739017348483 1.9075796724494945E-4 34-35 7.630318689797978E-4 9.537898362247472E-5 0.0 0.008250282083344064 1.9075796724494945E-4 36-37 7.630318689797978E-4 9.537898362247472E-5 0.0 0.010205551247604795 1.9075796724494945E-4 38-39 7.630318689797978E-4 1.4306847543371209E-4 0.0 0.01282847329722285 1.9075796724494945E-4 40-41 7.630318689797978E-4 1.9075796724494945E-4 0.0 0.01754973298653535 1.9075796724494945E-4 42-43 8.107213607910352E-4 1.9075796724494945E-4 0.0 0.02684918388972663 1.9075796724494945E-4 44-45 9.537898362247472E-4 1.9075796724494945E-4 0.0 0.043540506023659706 2.384474590561868E-4 46-47 0.0010968583116584593 1.9075796724494945E-4 0.0 0.057561216616163494 2.8613695086742417E-4 48-49 0.0011445478034696967 1.9075796724494945E-4 0.0 0.08946548663788129 2.8613695086742417E-4 50-51 0.0011445478034696967 1.9075796724494945E-4 0.0 0.12122668818416538 2.8613695086742417E-4 52-53 0.0011445478034696967 1.9075796724494945E-4 0.0 0.1567553595835372 2.8613695086742417E-4 54-55 0.0011445478034696967 1.9075796724494945E-4 0.0 0.2163672243475839 2.8613695086742417E-4 56-57 0.0012399267870921713 1.9075796724494945E-4 0.0 0.27545450470170696 2.8613695086742417E-4 58-59 0.0012399267870921713 1.9075796724494945E-4 0.0 0.35829115197782624 2.8613695086742417E-4 60-61 0.0012399267870921713 1.9075796724494945E-4 0.0 0.44441837418892094 2.8613695086742417E-4 62-63 0.0012399267870921713 1.9075796724494945E-4 0.0 0.5584916386014007 2.8613695086742417E-4 64-65 0.0012399267870921713 1.9075796724494945E-4 0.0 0.6514384581415023 2.8613695086742417E-4 66-67 0.0015737532297708328 2.8613695086742417E-4 0.0 0.780343154507277 2.8613695086742417E-4 68-69 0.0016214427215820702 3.815159344898989E-4 0.0 0.9569373426842889 2.8613695086742417E-4 70-71 0.0017168217052045448 4.2920542630113626E-4 0.0 1.0781640308684544 2.8613695086742417E-4 72-73 0.0018122006888270197 4.768949181123736E-4 0.0 1.2437896359288816 3.3382644267866153E-4 74-75 0.0018122006888270197 4.768949181123736E-4 0.0 1.4431317116998539 3.815159344898989E-4 76-77 0.0018122006888270197 4.768949181123736E-4 0.0 1.6088526957439035 3.815159344898989E-4 78-79 0.0018122006888270197 4.768949181123736E-4 0.0 1.7625559278515217 3.815159344898989E-4 80-81 0.0018122006888270197 4.768949181123736E-4 0.0 1.9702436646894603 4.2920542630113626E-4 82-83 0.0020506481478832065 4.768949181123736E-4 0.0 2.259098916590125 6.676528853573231E-4 84-85 0.0020983376396944437 4.768949181123736E-4 0.0 2.601461778302998 6.676528853573231E-4 86-87 0.0020983376396944437 4.768949181123736E-4 0.0 2.973678261889706 6.676528853573231E-4 88-89 0.0020983376396944437 4.768949181123736E-4 0.0 3.3578171184292227 6.676528853573231E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2765 0.0 40.39225 1 GTATCAA 4840 0.0 39.571724 1 TCAACGC 5880 0.0 32.168514 4 ATCAACG 5900 0.0 32.05947 3 CAACGCA 6000 0.0 31.525148 5 TATCAAC 6085 0.0 31.162882 2 AACGCAG 6215 0.0 30.583132 6 CGCAGAG 6885 0.0 27.26321 8 ACGCAGA 6960 0.0 27.105978 7 ACAGCGT 145 7.856361E-7 26.218378 8 GTACATG 4395 0.0 25.087328 1 TACATGG 4350 0.0 25.01909 2 GCAGAGT 7250 0.0 24.774004 9 GTATAGG 400 0.0 23.762674 1 GAGTACT 3700 0.0 23.305323 12-13 ACATGGG 4375 0.0 23.246683 3 GTACTTT 3990 0.0 21.78694 14-15 CATGGGA 2550 0.0 20.128384 4 AGTACTT 4065 0.0 19.86866 12-13 AGAGTAC 6245 0.0 19.85821 10-11 >>END_MODULE