FastQCFastQC Report
Thu 26 May 2016
SRR937133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048449
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49370.47088604214415775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39520.3769377432760201No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.22995872951378657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33700.041.1678121
GTATCAA56150.035.962011
CCGTTGC1252.1532833E-730.4005329
ATCAACG67700.029.6750033
TCAACGC68500.029.3284364
TATCAAC70500.028.7658922
CAACGCA70150.028.64275
AACGCAG72150.027.8487226
TCCGTTG1109.474718E-525.9095468
ACGCAGA81900.024.3014037
CGCAGAG82050.024.1990858
TTACGCG500.001652552723.75381780-81
GAGTACT47450.023.27157812-13
GTACATG44850.022.8821131
GCAGAGT85600.022.6960759
TACATGG45950.022.1190932
GTACTTT49950.021.72650314-15
AGAGTAC72700.020.90820910-11
AGTACTT49850.020.86498512-13
TACACCG1150.00372268420.6525365
Produced by FastQC (version 0.11.5)