##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937133_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1048449 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.079155972298125 31.0 31.0 34.0 30.0 34.0 2 32.20888665066207 33.0 31.0 34.0 30.0 34.0 3 32.3094408979359 34.0 31.0 34.0 30.0 34.0 4 35.98664980366236 37.0 35.0 37.0 35.0 37.0 5 35.88192558722456 37.0 35.0 37.0 35.0 37.0 6 35.91132711271602 37.0 35.0 37.0 35.0 37.0 7 35.85605976065598 37.0 35.0 37.0 35.0 37.0 8 35.82770358882502 37.0 35.0 37.0 35.0 37.0 9 37.48081594812909 39.0 37.0 39.0 35.0 39.0 10-11 37.39625866398842 39.0 37.0 39.0 34.0 39.0 12-13 37.293012821796765 39.0 37.0 39.0 34.0 39.0 14-15 38.476791431915146 40.0 38.0 41.0 34.0 41.0 16-17 38.39015440903658 40.0 38.0 41.0 33.5 41.0 18-19 38.54459253621302 40.0 38.0 41.0 34.0 41.0 20-21 38.56854649105488 40.0 38.0 41.0 34.0 41.0 22-23 38.47382848378891 40.0 38.0 41.0 34.0 41.0 24-25 38.365453159858035 40.0 38.0 41.0 34.0 41.0 26-27 38.1748535217259 40.0 38.0 41.0 33.0 41.0 28-29 38.08102587727204 40.0 37.5 41.0 33.0 41.0 30-31 38.0841447700365 40.0 37.0 41.0 33.0 41.0 32-33 37.92470258448432 40.0 37.0 41.0 33.0 41.0 34-35 37.76042468446248 40.0 37.0 41.0 33.0 41.0 36-37 37.609527025158116 40.0 37.0 41.0 33.0 41.0 38-39 37.449133434244295 40.0 37.0 41.0 32.5 41.0 40-41 37.2803674761481 40.0 36.0 41.0 32.0 41.0 42-43 37.02895658253287 39.5 36.0 41.0 31.0 41.0 44-45 36.740534828112764 39.0 36.0 41.0 31.0 41.0 46-47 36.355845157942824 39.0 35.5 41.0 30.0 41.0 48-49 35.976553461351 39.0 35.0 40.0 29.0 41.0 50-51 35.6699033524759 39.0 35.0 40.0 28.0 41.0 52-53 35.427035554423725 38.5 35.0 40.0 27.5 41.0 54-55 35.12729040706796 38.0 34.5 40.0 26.5 41.0 56-57 34.837573882945186 38.0 34.0 40.0 26.0 41.0 58-59 34.495887735121116 38.0 34.0 40.0 26.0 41.0 60-61 34.14823181671211 37.5 33.5 40.0 25.0 41.0 62-63 33.78158308129437 37.0 33.0 39.5 24.0 41.0 64-65 33.39848099430682 36.5 32.5 39.0 23.0 40.0 66-67 32.996875861391445 36.0 32.0 39.0 22.0 40.0 68-69 32.6876157066295 35.5 32.0 39.0 22.0 40.0 70-71 32.22305281420461 35.0 31.0 38.0 20.5 40.0 72-73 31.691284459234545 35.0 31.0 37.5 18.5 39.5 74-75 31.17420685221694 34.5 30.5 37.0 18.0 39.0 76-77 29.74211001202729 33.0 28.5 35.5 15.5 37.5 78-79 30.474629190356424 34.0 30.0 36.0 15.5 38.0 80-81 30.16077653753306 34.0 30.0 35.5 9.5 37.0 82-83 29.870042319655035 34.0 30.0 35.0 7.5 37.0 84-85 29.52964760326921 34.0 29.0 35.0 7.0 36.5 86-87 29.18822327075518 34.0 29.0 35.0 2.0 36.0 88-89 28.79055633607357 34.0 29.0 35.0 2.0 36.0 90-91 28.33576215915128 33.5 29.0 35.0 2.0 35.0 92-93 27.831561668712546 33.0 27.0 35.0 2.0 35.0 94-95 27.198914777924344 33.0 26.0 34.5 2.0 35.0 96-97 26.45981921867444 32.0 25.0 34.0 2.0 35.0 98-99 25.58289387466629 32.0 23.5 34.0 2.0 35.0 100-101 22.1744190704555 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24285242141967345 1101 2 0.23720148339971558 1101 3 0.2605438188402829 1101 4 0.2026760545828168 1101 5 0.21168691323283895 1101 6 0.26195007497889833 1101 7 0.2810054820669379 1101 8 0.3249617816608392 1101 9 0.3556763565747971 1101 10-11 0.44181629976708336 1101 12-13 0.40399375351349676 1101 14-15 0.5448787632662544 1101 16-17 0.5336112688762356 1101 18-19 0.3983668035063346 1101 20-21 0.3680379778332181 1101 22-23 0.4698227535931707 1101 24-25 0.46086428645570976 1101 26-27 0.35632997898641605 1101 28-29 0.41036397059484386 1101 30-31 0.5485987778899499 1101 32-33 0.5499008542245107 1101 34-35 0.6575627987327621 1101 36-37 0.6974952317961325 1101 38-39 0.6515416301532113 1101 40-41 0.6986430377650095 1101 42-43 0.8331799343357176 1101 44-45 0.8377968313217394 1101 46-47 0.8647455880223589 1101 48-49 1.0032962564448553 1101 50-51 0.9903442077744344 1101 52-53 0.9638033763781451 1101 54-55 0.9985563916965887 1101 56-57 0.9392291473706109 1101 58-59 0.8382665589127782 1101 60-61 0.8992514550462758 1101 62-63 0.7545714561921173 1101 64-65 1.0087875288619514 1101 66-67 0.7294304813864159 1101 68-69 0.7255044774803139 1101 70-71 0.749081073354354 1101 72-73 0.7846168033102394 1101 74-75 0.7326737965547601 1101 76-77 0.677016002234776 1101 78-79 0.8134195112214435 1101 80-81 1.0049544627331919 1101 82-83 1.100729204886843 1101 84-85 0.8850865841651121 1101 86-87 0.638718645701772 1101 88-89 0.5960503275178404 1101 90-91 0.8669376379527911 1101 92-93 0.8113066014134596 1101 94-95 0.7564371688040161 1101 96-97 0.8365778571691571 1101 98-99 0.9731996150755968 1101 100-101 1.4228477829033181 1102 1 0.3068454284366986 1102 2 0.29966262872392946 1102 3 0.3003664984075627 1102 4 0.17624915060352464 1102 5 0.296855790108026 1102 6 0.2098083880763184 1102 7 0.1810887155086931 1102 8 0.2827188064144863 1102 9 0.32873024536255 1102 10-11 0.3338996202204072 1102 12-13 0.3979739263230684 1102 14-15 0.46032383320180514 1102 16-17 0.50954712989585 1102 18-19 0.3203543425327453 1102 20-21 0.34885679110981016 1102 22-23 0.3388608864163416 1102 24-25 0.4785682799435804 1102 26-27 0.3481400143978419 1102 28-29 0.45010106969759534 1102 30-31 0.6085291457313886 1102 32-33 0.5133806074322109 1102 34-35 0.5319352563632762 1102 36-37 0.48689544726426703 1102 38-39 0.5611483378298132 1102 40-41 0.4984986672422451 1102 42-43 0.5551922602123867 1102 44-45 0.6704343582695671 1102 46-47 0.6498954024930725 1102 48-49 0.5496184717595796 1102 50-51 0.5706292737257712 1102 52-53 0.573696993277629 1102 54-55 0.6518355832216614 1102 56-57 0.6480091305865301 1102 58-59 0.7124383747184311 1102 60-61 0.6975091054818492 1102 62-63 0.6481022027628924 1102 64-65 0.6215324922083241 1102 66-67 0.6320075696356184 1102 68-69 0.48427604576093586 1102 70-71 0.42370758188893376 1102 72-73 0.6572905316532278 1102 74-75 0.6285604387894921 1102 76-77 0.6836532109034223 1102 78-79 0.7399963384359189 1102 80-81 0.9089374748678694 1102 82-83 0.7330812537232347 1102 84-85 0.6362709719986057 1102 86-87 0.5283181291277828 1102 88-89 0.720001179716089 1102 90-91 0.8392018394441223 1102 92-93 0.7036143201416714 1102 94-95 0.4499935608919152 1102 96-97 0.6138708697961412 1102 98-99 0.9298234857855547 1102 100-101 1.2047572748358597 1103 1 0.23862156303620097 1103 2 0.1986928978055431 1103 3 0.23102948987631322 1103 4 0.09975285468723882 1103 5 0.1307150760603264 1103 6 0.10318281781288619 1103 7 0.05023466181438607 1103 8 0.13567853776269345 1103 9 0.16919513571437506 1103 10-11 0.2519281945144485 1103 12-13 0.2527962139581419 1103 14-15 0.22923432558682322 1103 16-17 0.3439893312617599 1103 18-19 0.32878749363134574 1103 20-21 0.2764570791358807 1103 22-23 0.30779836969146857 1103 24-25 0.32372422397076406 1103 26-27 0.3208054988265445 1103 28-29 0.31770786511635407 1103 30-31 0.3144823470404461 1103 32-33 0.32796266690183273 1103 34-35 0.4622354422029531 1103 36-37 0.3040320512094681 1103 38-39 0.28982645898656045 1103 40-41 0.41857975083405563 1103 42-43 0.34848109555085216 1103 44-45 0.403422702734332 1103 46-47 0.33059582995666403 1103 48-49 0.5251341756972465 1103 50-51 0.44281620135367206 1103 52-53 0.6817623472811718 1103 54-55 0.5062043638203946 1103 56-57 0.6230960537313592 1103 58-59 0.49941704960438216 1103 60-61 0.44153344257588145 1103 62-63 0.5267856620151932 1103 64-65 0.43599149817635663 1103 66-67 0.42078987377950483 1103 68-69 0.31015141255280554 1103 70-71 0.4164447678020906 1103 72-73 0.40097031894282864 1103 74-75 0.5643107970889361 1103 76-77 0.3576239405771773 1103 78-79 0.2841549829386274 1103 80-81 0.5521499130464811 1103 82-83 0.5012433728408752 1103 84-85 0.47702714674243296 1103 86-87 0.25010744951658026 1103 88-89 0.30075963297170105 1103 90-91 0.4293494481852882 1103 92-93 0.3987760182199125 1103 94-95 0.21250989292238387 1103 96-97 0.4446517384313857 1103 98-99 0.27641713377535737 1103 100-101 0.7480721073929715 1104 1 0.2827063179180165 1104 2 0.2969247809028559 1104 3 0.28497249619232434 1104 4 0.09938054129848695 1104 5 0.08105057626584511 1104 6 0.12881235112331524 1104 7 0.22245717141105814 1104 8 0.23696667394390403 1104 9 0.3090242122749487 1104 10-11 0.35765652406321635 1104 12-13 0.28122553156222097 1104 14-15 0.2523905457448379 1104 16-17 0.2385870082368342 1104 18-19 0.26914254803340043 1104 20-21 0.2999448812262244 1104 22-23 0.21777720482137397 1104 24-25 0.33910646623279206 1104 26-27 0.17580120421328616 1104 28-29 0.2939075304059955 1104 30-31 0.4970865769789583 1104 32-33 0.44583113867926016 1104 34-35 0.4308764023130678 1104 36-37 0.44680664704655726 1104 38-39 0.5349446468053358 1104 40-41 0.6545412594528557 1104 42-43 0.5735702478812357 1104 44-45 0.7704804946309736 1104 46-47 0.6979689421775603 1104 48-49 0.6311267176591642 1104 50-51 0.8811791313299508 1104 52-53 0.9028238864718006 1104 54-55 0.8275860863304487 1104 56-57 0.8499293796824361 1104 58-59 1.039445075324359 1104 60-61 1.0235780563750794 1104 62-63 1.1318176849933153 1104 64-65 0.8078605578496436 1104 66-67 0.9593771210237847 1104 68-69 0.9328799369311156 1104 70-71 0.8759669924644129 1104 72-73 1.057751115253641 1104 74-75 1.0947904711629484 1104 76-77 0.8976734935688029 1104 78-79 1.0811007741711727 1104 80-81 1.24263033670319 1104 82-83 1.1836489014159248 1104 84-85 1.1273951391896126 1104 86-87 1.009984053337039 1104 88-89 0.9970576856363884 1104 90-91 1.145887162881106 1104 92-93 1.1154340688155067 1104 94-95 1.1836447642218246 1104 96-97 1.0671279755758114 1104 98-99 0.9060427481931157 1104 100-101 1.0657519135422113 1105 1 0.13181593741236597 1105 2 0.197773668447347 1105 3 0.15509695637474152 1105 4 0.2017342090658616 1105 5 0.12787099987274786 1105 6 0.1849089645028883 1105 7 0.17400040567400765 1105 8 0.0616125110661514 1105 9 0.25263258978562675 1105 10-11 0.162816799099609 1105 12-13 0.1564373794272811 1105 14-15 0.06607522822667278 1105 16-17 0.09387872829890398 1105 18-19 0.0751740583725109 1105 20-21 0.08201987525357879 1105 22-23 0.1631698965433941 1105 24-25 0.11395911362397015 1105 26-27 0.18162751724928228 1105 28-29 0.09772483607985549 1105 30-31 0.15824009532492767 1105 32-33 0.16876639049127817 1105 34-35 0.15150487924789502 1105 36-37 0.16500048065487505 1105 38-39 0.04768170018328988 1105 40-41 0.31797069593871186 1105 42-43 0.3164959902078408 1105 44-45 0.4723518154910096 1105 46-47 0.25923943560951557 1105 48-49 0.07904148013007983 1105 50-51 0.11215092686946804 1105 52-53 0.015029736441668717 1105 54-55 0.13907526519994207 1105 56-57 0.03948582343410578 1105 58-59 0.15822738431531036 1105 60-61 0.22057471402938944 1105 62-63 0.3405366301417203 1105 64-65 0.1709520195566654 1105 66-67 0.28660837871166933 1105 68-69 0.2424309854290243 1105 70-71 0.06558748732232544 1105 72-73 0.1954462665848986 1105 74-75 0.26681631414797025 1105 76-77 -0.041210530448960725 1105 78-79 0.14365556241213895 1105 80-81 0.001374832165144113 1105 82-83 0.23908723773285345 1105 84-85 0.04856996738537944 1105 86-87 -0.21828403747714376 1105 88-89 -0.12088541360495597 1105 90-91 0.016880988260957963 1105 92-93 -0.08239455193041678 1105 94-95 0.11038549329172298 1105 96-97 -0.012169164051396564 1105 98-99 -0.13654422455991622 1105 100-101 -0.059358130466041104 1106 1 0.1559808675495411 1106 2 0.07352658645817911 1106 3 0.04425675961531539 1106 4 0.09129093333333316 1106 5 0.03851989149777779 1106 6 0.09890333335458479 1106 7 0.06806636881839978 1106 8 0.1570227404230522 1106 9 0.09898905051913687 1106 10-11 0.10889730521441265 1106 12-13 0.21181866325078857 1106 14-15 0.18517786268872527 1106 16-17 0.14877972417915686 1106 18-19 -0.07861191343934593 1106 20-21 0.14810385105219126 1106 22-23 0.1009039230034503 1106 24-25 0.16586227569398204 1106 26-27 0.07811145848606316 1106 28-29 0.2492642888823653 1106 30-31 0.15463405429490962 1106 32-33 0.2094190690669322 1106 34-35 0.1405665997925425 1106 36-37 0.13890562327980405 1106 38-39 0.49519631977026535 1106 40-41 0.43686069413635664 1106 42-43 0.2917333159051765 1106 44-45 0.2032292337222401 1106 46-47 0.38117013238817776 1106 48-49 0.4828762232755963 1106 50-51 0.4486954053132237 1106 52-53 0.44061480681077825 1106 54-55 0.35186273761629394 1106 56-57 0.5101342560348243 1106 58-59 0.33963919460595804 1106 60-61 0.42330415362145146 1106 62-63 0.5603390741761913 1106 64-65 0.17789037461602675 1106 66-67 0.30127893088417323 1106 68-69 0.3014625251248475 1106 70-71 0.18412072912080646 1106 72-73 0.23465740629502463 1106 74-75 0.5280541407218351 1106 76-77 0.41031920183860393 1106 78-79 0.5475436333826984 1106 80-81 0.458387879931351 1106 82-83 0.5412848841531748 1106 84-85 0.5208816880987825 1106 86-87 0.6995472346344975 1106 88-89 0.6901113239472174 1106 90-91 0.5600427747565995 1106 92-93 0.6097402014081439 1106 94-95 0.5575656326596068 1106 96-97 0.4698293198182917 1106 98-99 0.2426915404547323 1106 100-101 -0.1034200771476641 1107 1 0.06711103717027456 1107 2 0.11460311998754236 1107 3 0.10907475084840002 1107 4 0.10543234747474628 1107 5 0.01123355100389034 1107 6 0.05877155737374551 1107 7 -0.11419560400960904 1107 8 0.10368613991663977 1107 9 0.10923163812021386 1107 10-11 0.1611639065376238 1107 12-13 0.0671686591668319 1107 14-15 0.1619346488794733 1107 16-17 0.06125079608419526 1107 18-19 0.013299110547151827 1107 20-21 -0.04399231274208404 1107 22-23 0.14502981807274296 1107 24-25 0.23043795159041025 1107 26-27 0.10556524160022462 1107 28-29 0.3549124143418041 1107 30-31 0.2682826698303131 1107 32-33 0.24418587458844598 1107 34-35 0.2725249977886719 1107 36-37 0.3131848032193645 1107 38-39 0.28286410272106366 1107 40-41 0.48553476639533955 1107 42-43 0.3308794946739937 1107 44-45 0.3728526920249493 1107 46-47 0.2684472158916833 1107 48-49 0.4235443601701476 1107 50-51 0.43000717530423316 1107 52-53 0.4442141424855848 1107 54-55 0.3702256116395475 1107 56-57 0.5824735519590263 1107 58-59 0.546452611796731 1107 60-61 0.4933645963234099 1107 62-63 0.4848850732504886 1107 64-65 0.27668288189909873 1107 66-67 0.565187558943741 1107 68-69 0.38090247720630543 1107 70-71 0.5063797070401677 1107 72-73 0.4354537797329101 1107 74-75 0.3570993093896426 1107 76-77 0.33118282458902115 1107 78-79 0.4939143486213382 1107 80-81 0.4914407809087784 1107 82-83 0.5632253126973765 1107 84-85 0.7242482376917501 1107 86-87 0.6027068632658228 1107 88-89 0.5110579873467138 1107 90-91 0.5781047965922035 1107 92-93 0.5195213008118849 1107 94-95 0.3948291029419444 1107 96-97 0.42838931328394025 1107 98-99 0.2755143779945506 1107 100-101 0.6413347487392365 1108 1 0.02569922855292006 1108 2 -0.07832150734817134 1108 3 -0.11211170959902717 1108 4 -0.06806241539646152 1108 5 0.007141825434104021 1108 6 -0.10032518370913834 1108 7 -0.11675234929153078 1108 8 -0.11287905008965993 1108 9 -0.06413248813075256 1108 10-11 -0.07831751273594278 1108 12-13 -0.1248076862737193 1108 14-15 -0.21783067176344417 1108 16-17 -0.22467310808450947 1108 18-19 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-0.4938140285455681 2316 98-99 -0.9289461147836988 2316 100-101 -1.1596375914854384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 6.0 5 27.0 6 51.0 7 152.0 8 320.0 9 629.0 10 1054.0 11 1625.0 12 2630.0 13 3545.0 14 4553.0 15 6286.0 16 8461.0 17 11388.0 18 10436.0 19 6697.0 20 5781.0 21 6135.0 22 6431.0 23 7276.0 24 8379.0 25 9891.0 26 11919.0 27 14838.0 28 18233.0 29 22552.0 30 28014.0 31 34574.0 32 43493.0 33 55777.0 34 74354.0 35 103602.0 36 155748.0 37 224802.0 38 149748.0 39 9039.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.00023279821547 20.410488132061467 15.669323492202214 21.919955577520852 2 15.357966896443841 21.396843019934053 40.13584419028107 23.10934589334104 3 18.961932303748018 25.88158878647296 30.430407612013184 24.726071297765838 4 12.935990088197114 17.911444259938747 37.794089734626546 31.358475917237588 5 13.412860329877752 38.05287620094063 34.20891240298765 14.32535106619397 6 30.45145734318026 37.530199370689466 17.819273994252463 14.19906929187781 7 27.192071335849434 33.75490844094467 21.453117891285125 17.59990233192077 8 25.859436176676216 35.4496022219488 20.010129248060707 18.680832353314276 9 26.229983528049527 17.085809610195632 20.91260519109656 35.77160167065828 10-11 24.266453757015906 27.58896990648001 27.607475494404444 20.53710084209964 12-13 25.475297320136697 24.936692199620584 28.296846103148553 21.291164377094166 14-15 22.64301840146731 26.114336510407277 26.82786668688701 24.4147784012384 16-17 21.357548149695408 29.449882636160652 27.35378640258134 21.838782811562606 18-19 21.378619997005092 27.707981423953715 30.161944285214226 20.751454293826967 20-21 22.423791537903934 26.732144900995763 30.23177291976651 20.612290641333793 22-23 21.903867596184387 26.639381398263495 30.284501859448753 21.17224914610336 24-25 21.79929145530197 26.888307867822263 30.28432406858041 21.028076608295354 26-27 21.67002259648815 27.012055593656196 30.24042510776016 21.077496702095495 28-29 21.475634368645345 27.13845470048949 30.246059875396302 21.139851055468867 30-31 21.683842454885834 26.96500984573505 30.216844624333582 21.134303075045533 32-33 21.62903691947002 27.013201808059193 30.08812333260525 21.26963793986553 34-35 21.78093545799557 26.986863452585673 30.0467643156701 21.185436773748652 36-37 21.948178739327908 26.80054962376717 30.02885000145466 21.222421635450257 38-39 21.762391254578755 27.14567116910867 29.89430708180708 21.197630494505496 40-41 21.76192613145105 27.11662574091975 29.9049972649933 21.216450862635895 42-43 21.586743847340216 27.188017729045477 29.683656525019337 21.541581898594973 44-45 21.80654471509821 27.295795980538873 29.46585861591742 21.431800688445506 46-47 22.0838640789015 27.47693849900782 28.988555925361954 21.450641496728732 48-49 22.25799574882692 27.591849231674626 28.56670035238858 21.583454667109876 50-51 22.092493091729057 27.865966666698462 28.45134545551665 21.590194786055832 52-53 22.244620386876232 27.79353120657276 28.331897879629814 21.629950526921196 54-55 22.187964241344694 27.798849217988064 28.273469300159526 21.739717240507716 56-57 22.20001421185488 28.087585086327483 28.167944064428347 21.544456637389292 58-59 22.23963954254179 27.775358852701455 28.2715696714803 21.713431933276457 60-61 22.151539545947927 27.672577749884113 28.621142550833344 21.554740153334617 62-63 21.908850673001396 27.65322031036803 28.839169763845018 21.598759252785552 64-65 21.947488096691494 27.719352795751433 28.591117537541205 21.74204157001587 66-67 21.938577933610446 27.805369614027992 28.346518278015065 21.909534174346494 68-69 21.947703254358856 27.715510663461906 28.463860592880852 21.872925489298385 70-71 22.022024265702818 27.60401341237817 28.430817566571875 21.943144755347138 72-73 22.025057966577297 27.623279720806636 28.23938026551601 22.11228204710005 74-75 21.942433005738003 27.981597561347822 27.977687019289466 22.09828241362471 76-77 22.0759903438317 27.84060073499045 28.094642657868906 21.988766263308946 78-79 22.058917508810918 27.857623174029367 27.888526966897647 22.19493235026206 80-81 22.067677205084234 27.945103956627985 27.81042484963059 22.176793988657195 82-83 22.1198163276111 27.937921136490797 27.81877352366425 22.123489012233854 84-85 21.99129772455958 27.970856963799662 27.71307005842057 22.32477525322019 86-87 21.90107141239004 27.837788211840618 27.81212963779918 22.44901073797016 88-89 22.05299838713831 27.75366804425975 27.647987928812807 22.545345639789137 90-91 21.98648089260064 27.862819307124287 27.79905803086866 22.351641769406413 92-93 21.80544606796068 27.900666177370383 27.707332147767477 22.586555606901463 94-95 21.859167073617385 27.943016725674518 27.543139955438896 22.654676245269197 96-97 22.01362811313394 27.98308122405026 27.36152747798833 22.641763184827468 98-99 21.839662616288024 27.986099089058253 27.20907307707165 22.96516521758207 100-101 21.711835275580338 27.96268176971163 27.230023215399612 23.09545973930842 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 240.0 1 181.0 2 139.5 3 166.5 4 155.5 5 160.5 6 249.0 7 493.0 8 909.0 9 1170.0 10 1106.0 11 1064.0 12 1153.5 13 1243.0 14 1394.5 15 1566.5 16 1669.0 17 1736.5 18 1813.5 19 1953.5 20 2181.0 21 2556.0 22 3038.0 23 3666.5 24 4477.0 25 5373.5 26 6812.5 27 8627.0 28 10148.0 29 11904.5 30 14038.0 31 15988.0 32 18051.5 33 20260.5 34 23059.5 35 25505.5 36 28323.5 37 30866.5 38 32084.5 39 32923.0 40 33578.5 41 34685.0 42 35256.5 43 36275.5 44 37997.5 45 39074.0 46 39177.0 47 39071.0 48 39825.5 49 39192.0 50 37154.0 51 35804.0 52 34740.0 53 33905.5 54 32741.0 55 30089.5 56 26571.5 57 23649.5 58 21530.0 59 19276.5 60 16074.5 61 12603.5 62 9459.5 63 6939.5 64 4993.0 65 3429.5 66 2310.0 67 1690.5 68 1185.0 69 712.0 70 471.5 71 282.0 72 149.5 73 87.0 74 56.0 75 36.5 76 23.5 77 10.5 78 3.5 79 2.0 80 1.0 81 0.5 82 1.5 83 1.5 84 0.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03157044357903913 2 0.004005917312143938 3 9.537898362247471E-5 4 1.9075796724494942E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011159341083829544 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.9075796724494942E-4 20-21 8.584108526022725E-4 22-23 0.0019075796724494945 24-25 0.009824035313114897 26-27 0.0053412230828585846 28-29 4.768949181123736E-4 30-31 0.0024321640823731054 32-33 0.0030521274759191907 34-35 0.0 36-37 0.009108692935946336 38-39 0.01230388888729924 40-41 5.245844099236109E-4 42-43 0.0 44-45 0.0 46-47 2.384474590561868E-4 48-49 0.003099816967730429 50-51 0.006485770886328282 52-53 0.0 54-55 0.003004437984107954 56-57 0.0027183010332405295 58-59 3.3382644267866153E-4 60-61 0.002193716623316919 62-63 0.004625880705690024 64-65 9.537898362247471E-5 66-67 0.0029567484922967163 68-69 8.584108526022725E-4 70-71 0.0013829952625258833 72-73 0.0 74-75 9.537898362247471E-5 76-77 0.0 78-79 0.003242885443164141 80-81 0.0029567484922967163 82-83 0.016166737724009464 84-85 0.00977634582130366 86-87 0.006342702410894569 88-89 1.9075796724494942E-4 90-91 6.676528853573231E-4 92-93 4.2920542630113626E-4 94-95 9.537898362247471E-5 96-97 0.0026706115414292923 98-99 0.0027183010332405295 100-101 6.676528853573231E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1048449.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.14088934112657 #Duplication Level Percentage of deduplicated Percentage of total 1 81.23358203831248 52.10394196302728 2 11.231608230856532 14.408106813165103 3 3.288379868040079 6.327588276826413 4 1.3617316319124029 3.493707116592205 5 0.7554179229524229 2.4226588701197516 6 0.4615084708115203 1.7760938253788556 7 0.3044833102071401 1.3670881214351258 8 0.22046176014729513 1.1312490689246115 9 0.17393764692572714 1.0040863827347104 >10 0.893045509345971 10.249540498729496 >50 0.055480147684516756 2.43249976860945 >100 0.01947163301146262 1.9934218081651374 >500 2.9727659746467346E-4 0.11688429814454894 >1k 5.945531949293469E-4 1.1731331881473694 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4937 0.47088604214415775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3952 0.3769377432760201 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2411 0.22995872951378657 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 9.537898362247472E-5 0.0 0.0 0.0 0.0 3 9.537898362247472E-5 0.0 0.0 0.0 0.0 4 9.537898362247472E-5 0.0 0.0 0.0 0.0 5 9.537898362247472E-5 0.0 0.0 0.0 0.0 6 9.537898362247472E-5 0.0 0.0 0.0 0.0 7 9.537898362247472E-5 0.0 0.0 0.0 0.0 8 9.537898362247472E-5 0.0 0.0 0.0 0.0 9 9.537898362247472E-5 0.0 0.0 0.0 0.0 10-11 9.537898362247472E-5 0.0 0.0 0.0 0.0 12-13 9.537898362247472E-5 0.0 0.0 0.0 9.537898362247472E-5 14-15 9.537898362247472E-5 9.537898362247472E-5 0.0 0.0 9.537898362247472E-5 16-17 9.537898362247472E-5 9.537898362247472E-5 0.0 4.768949181123736E-5 1.9075796724494945E-4 18-19 9.537898362247472E-5 9.537898362247472E-5 0.0 9.537898362247472E-5 1.9075796724494945E-4 20-21 9.537898362247472E-5 9.537898362247472E-5 0.0 9.537898362247472E-5 1.9075796724494945E-4 22-23 1.9075796724494945E-4 9.537898362247472E-5 0.0 1.4306847543371209E-4 1.9075796724494945E-4 24-25 1.9075796724494945E-4 9.537898362247472E-5 0.0 4.768949181123736E-4 1.9075796724494945E-4 26-27 1.9075796724494945E-4 9.537898362247472E-5 0.0 9.061003444135098E-4 1.9075796724494945E-4 28-29 3.3382644267866153E-4 9.537898362247472E-5 0.0 0.0019075796724494945 1.9075796724494945E-4 30-31 3.815159344898989E-4 9.537898362247472E-5 0.0 0.003385953918597853 1.9075796724494945E-4 32-33 3.815159344898989E-4 9.537898362247472E-5 0.0 0.005818118000970958 2.8613695086742417E-4 34-35 3.815159344898989E-4 9.537898362247472E-5 0.0 0.008250282083344064 3.815159344898989E-4 36-37 4.768949181123736E-4 9.537898362247472E-5 0.0 0.010253240739416032 3.815159344898989E-4 38-39 4.768949181123736E-4 9.537898362247472E-5 0.0 0.013114610248090275 3.815159344898989E-4 40-41 4.768949181123736E-4 9.537898362247472E-5 0.0 0.017978938412836487 3.815159344898989E-4 42-43 6.676528853573231E-4 9.537898362247472E-5 0.0 0.027612215758706433 3.815159344898989E-4 44-45 8.584108526022725E-4 9.537898362247472E-5 0.0 0.04473274331894064 3.815159344898989E-4 46-47 8.584108526022725E-4 9.537898362247472E-5 0.0 0.0592303488295568 3.815159344898989E-4 48-49 9.537898362247472E-4 9.537898362247472E-5 0.0 0.09137306631033079 3.815159344898989E-4 50-51 9.537898362247472E-4 9.537898362247472E-5 0.0 0.12289582039755868 3.815159344898989E-4 52-53 9.537898362247472E-4 9.537898362247472E-5 0.0 0.15766145992795072 4.768949181123736E-4 54-55 9.537898362247472E-4 1.9075796724494945E-4 0.0 0.21698718774113 4.768949181123736E-4 56-57 0.0011445478034696967 1.9075796724494945E-4 0.0 0.2764082945379317 4.768949181123736E-4 58-59 0.0011445478034696967 1.9075796724494945E-4 0.0 0.35938801028948475 4.768949181123736E-4 60-61 0.0011445478034696967 1.9075796724494945E-4 0.0 0.445038337582467 4.768949181123736E-4 62-63 0.0011445478034696967 1.9075796724494945E-4 0.0 0.5582055016505333 4.768949181123736E-4 64-65 0.0011445478034696967 1.9075796724494945E-4 0.0 0.6514384581415024 4.768949181123736E-4 66-67 0.0011445478034696967 1.9075796724494945E-4 0.0 0.7805816019663332 4.768949181123736E-4 68-69 0.0011445478034696967 1.9075796724494945E-4 0.0 0.9567942742088552 4.768949181123736E-4 70-71 0.0011445478034696967 1.9075796724494945E-4 0.0 1.0774009989994746 4.768949181123736E-4 72-73 0.0011445478034696967 1.9075796724494945E-4 0.0 1.2417866772728097 4.768949181123736E-4 74-75 0.0011445478034696967 1.9075796724494945E-4 0.0 1.4400318947321233 4.768949181123736E-4 76-77 0.0011445478034696967 1.9075796724494945E-4 0.0 1.6046560204645148 4.768949181123736E-4 78-79 0.0011445478034696967 1.9075796724494945E-4 0.0 1.757596220703153 4.768949181123736E-4 80-81 0.0011445478034696967 1.9075796724494945E-4 0.0 1.9647116836393568 5.245844099236109E-4 82-83 0.0011445478034696967 1.9075796724494945E-4 0.0 2.253090040621909 6.676528853573231E-4 84-85 0.0012399267870921715 1.9075796724494945E-4 0.0 2.594785249449425 6.676528853573231E-4 86-87 0.0013353057707146461 1.9075796724494945E-4 0.0 2.9672878699869996 7.153423771685604E-4 88-89 0.0013353057707146461 1.9075796724494945E-4 0.0 3.3517605529691954 7.630318689797978E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3370 0.0 41.167812 1 GTATCAA 5615 0.0 35.96201 1 CCGTTGC 125 2.1532833E-7 30.400532 9 ATCAACG 6770 0.0 29.675003 3 TCAACGC 6850 0.0 29.328436 4 TATCAAC 7050 0.0 28.765892 2 CAACGCA 7015 0.0 28.6427 5 AACGCAG 7215 0.0 27.848722 6 TCCGTTG 110 9.474718E-5 25.909546 8 ACGCAGA 8190 0.0 24.301403 7 CGCAGAG 8205 0.0 24.199085 8 TTACGCG 50 0.0016525527 23.753817 80-81 GAGTACT 4745 0.0 23.271578 12-13 GTACATG 4485 0.0 22.882113 1 GCAGAGT 8560 0.0 22.696075 9 TACATGG 4595 0.0 22.119093 2 GTACTTT 4995 0.0 21.726503 14-15 AGAGTAC 7270 0.0 20.908209 10-11 AGTACTT 4985 0.0 20.864985 12-13 TACACCG 115 0.003722684 20.652536 5 >>END_MODULE