FastQCFastQC Report
Thu 26 May 2016
SRR937132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098042
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46900.42712391693578206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33210.3024474473654013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21340.19434593576566286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25950.043.6099621
GTATCAA48400.042.047921
TATCAAC59950.033.7898562
ATCAACG60100.033.784643
TCAACGC61350.032.941274
CAACGCA62350.032.4892045
AACGCAG64650.031.3290736
ACGCAGA73100.027.4424157
TCGATCG350.00841963127.1253738-39
CGCAGAG73000.027.0892968
GCAGAGT76950.024.7103359
GAGTACT38900.023.5854912-13
GTACATG47700.023.1268081
TACATGG47500.022.924922
ACATGGG46200.022.6436613
AGAGTAC65650.021.68698910-11
GTATTAG3750.021.5557821
GTACTTT42350.021.54990614-15
AGTACTT41750.021.00763112-13
ATGTGCG1150.00370663620.6680457
Produced by FastQC (version 0.11.5)