FastQCFastQC Report
Thu 26 May 2016
SRR937132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098042
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50940.4639166807827023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.38350081326579494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.23523690350642323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33450.041.0540961
GTATCAA56600.037.1074071
ATCAACG70550.029.2866023
TATCAAC71600.028.9247762
TCAACGC71450.028.9177024
CAACGCA72750.028.400965
AACGCAG74550.027.7177456
GTGTACG1351.3388015E-524.638771
ACGCAGA84000.024.4298487
CGCAGAG83950.024.2768578
TACATGG47900.023.5022452
GTACATG47800.023.4605871
TGAACCG1703.1498348E-622.3520745
GCAGAGT88550.022.0500289
TTGGACG1302.942165E-421.9222264
GGCGCGC2205.630136E-821.5940068
ACATGGG48650.021.0885963
GGGCGCG2056.82523E-720.854757
AGAGTAC76350.020.4080210-11
GAGTACT49000.020.4066812-13
Produced by FastQC (version 0.11.5)