##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937132_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1098042 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.003460705510356 31.0 31.0 34.0 30.0 34.0 2 32.14188801521253 33.0 31.0 34.0 30.0 34.0 3 32.25765590022968 34.0 31.0 34.0 30.0 34.0 4 35.955489862864994 37.0 35.0 37.0 35.0 37.0 5 35.76505816717393 37.0 35.0 37.0 35.0 37.0 6 35.85619311465317 37.0 35.0 37.0 35.0 37.0 7 35.812227583280055 37.0 35.0 37.0 35.0 37.0 8 35.777910134585014 37.0 35.0 37.0 33.0 37.0 9 37.43503436116287 39.0 37.0 39.0 34.0 39.0 10-11 37.3395922013912 39.0 37.0 39.0 34.0 39.0 12-13 37.23350928288717 39.0 37.0 39.0 33.0 39.0 14-15 38.39475311509032 40.0 38.0 41.0 33.5 41.0 16-17 38.385199746457786 40.0 38.0 41.0 33.5 41.0 18-19 38.507544338012565 40.0 38.0 41.0 34.0 41.0 20-21 38.50771828400006 40.0 38.0 41.0 34.0 41.0 22-23 38.4109096009078 40.0 38.0 41.0 34.0 41.0 24-25 38.31173670952477 40.0 38.0 41.0 34.0 41.0 26-27 38.107536870174364 40.0 37.5 41.0 33.0 41.0 28-29 37.939689465430284 40.0 37.0 41.0 33.0 41.0 30-31 37.92507663641281 40.0 37.0 41.0 33.0 41.0 32-33 37.80296245498806 40.0 37.0 41.0 33.0 41.0 34-35 37.67015833638422 40.0 37.0 41.0 33.0 41.0 36-37 37.523087003957954 40.0 37.0 41.0 33.0 41.0 38-39 37.346172095420755 40.0 36.0 41.0 32.0 41.0 40-41 36.94600297620674 39.0 36.0 41.0 30.5 41.0 42-43 36.7802388251087 39.0 36.0 41.0 30.5 41.0 44-45 36.56866312946135 39.0 35.5 41.0 30.0 41.0 46-47 36.174307995504726 39.0 35.0 40.0 30.0 41.0 48-49 35.79760928999073 39.0 35.0 40.0 28.5 41.0 50-51 35.510987284639384 39.0 35.0 40.0 27.5 41.0 52-53 35.2561227166174 38.0 34.5 40.0 27.0 41.0 54-55 34.88901927248685 38.0 34.0 40.0 26.0 41.0 56-57 34.66084858320538 38.0 34.0 40.0 26.0 41.0 58-59 34.333741332298764 38.0 34.0 40.0 25.5 41.0 60-61 33.93890944062249 37.0 33.0 40.0 24.5 41.0 62-63 33.555659983862185 37.0 33.0 39.0 23.0 40.5 64-65 33.241431566369954 36.0 32.0 39.0 23.0 40.0 66-67 32.90234526548165 36.0 32.0 39.0 22.0 40.0 68-69 32.440634329105805 35.0 31.0 38.5 20.5 40.0 70-71 31.979223472326193 35.0 31.0 38.0 20.0 40.0 72-73 31.55463179004082 35.0 31.0 37.0 18.5 39.0 74-75 31.064066310760424 34.5 30.0 37.0 17.5 39.0 76-77 29.6132875609494 33.0 28.5 35.0 13.5 37.5 78-79 30.28850717914251 34.0 30.0 36.0 13.5 38.0 80-81 30.162919086883743 34.0 30.0 35.5 11.0 37.0 82-83 29.809707187885344 34.0 29.5 35.0 7.5 37.0 84-85 29.356943541321733 34.0 29.0 35.0 6.5 36.0 86-87 28.951143945313568 34.0 29.0 35.0 2.0 36.0 88-89 28.55546053793935 33.5 29.0 35.0 2.0 35.5 90-91 28.069107556905838 33.0 27.5 35.0 2.0 35.0 92-93 27.519107192621046 33.0 26.5 34.5 2.0 35.0 94-95 26.92689623894168 32.5 25.5 34.0 2.0 35.0 96-97 26.117555612626838 32.0 25.0 34.0 2.0 35.0 98-99 25.209472861693815 32.0 21.5 34.0 2.0 35.0 100-101 22.124349068614862 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20824122045300086 1101 2 0.3653787870651186 1101 3 0.25109595322324907 1101 4 0.17694465430019335 1101 5 0.08370966462162244 1101 6 0.16568501470612063 1101 7 0.1628355353294353 1101 8 0.24612574315555236 1101 9 0.29199822272510545 1101 10-11 0.33512613654948353 1101 12-13 0.35314145634410465 1101 14-15 0.4674013267069057 1101 16-17 0.47730129282822276 1101 18-19 0.3256224998451671 1101 20-21 0.26201602504939814 1101 22-23 0.3056425034310948 1101 24-25 0.2667864429647082 1101 26-27 0.2596282651556123 1101 28-29 0.33733243350204134 1101 30-31 0.4008987357961544 1101 32-33 0.4507736297007128 1101 34-35 0.35083594449543654 1101 36-37 0.4897634926454941 1101 38-39 0.5188677071175931 1101 40-41 0.7011152614547242 1101 42-43 0.7643870451620174 1101 44-45 0.5934798876931779 1101 46-47 0.4185544700787034 1101 48-49 0.7046891711600622 1101 50-51 0.7439912353428539 1101 52-53 0.7734026746679348 1101 54-55 0.6071524901654399 1101 56-57 0.6246813197402901 1101 58-59 0.6248812512981203 1101 60-61 0.5684259897438579 1101 62-63 0.7265222446746193 1101 64-65 0.6464656504191666 1101 66-67 0.6608028713531979 1101 68-69 0.4053881164494868 1101 70-71 0.4294249523208684 1101 72-73 0.3962012464436775 1101 74-75 0.3370458027470207 1101 76-77 0.5274992634228362 1101 78-79 0.6393378717840292 1101 80-81 0.7017374015268913 1101 82-83 0.9295405174473998 1101 84-85 0.674775039405251 1101 86-87 0.4596213071875148 1101 88-89 0.5415236068898999 1101 90-91 0.7349309054536377 1101 92-93 0.7734736191522735 1101 94-95 0.7965182462555411 1101 96-97 0.6005443072912193 1101 98-99 0.6925173915168479 1101 100-101 0.9618506432904859 1102 1 -0.10401257470799052 1102 2 -0.0010766352885127617 1102 3 0.0875963072783037 1102 4 0.030970211896594435 1102 5 -0.11051414483624455 1102 6 0.04488120255845729 1102 7 0.08239324061260334 1102 8 0.16822775084593644 1102 9 0.20556554032320662 1102 10-11 0.23625875231353177 1102 12-13 0.1782380698402335 1102 14-15 0.19329480592900694 1102 16-17 0.17521010122098346 1102 18-19 0.27320452101866977 1102 20-21 0.22358165593036006 1102 22-23 0.09654790747021735 1102 24-25 0.05043841320093634 1102 26-27 0.2335068173483279 1102 28-29 -0.050373026078105454 1102 30-31 -0.04384856867164899 1102 32-33 -0.054051117315424335 1102 34-35 0.08976411276653096 1102 36-37 0.14015020271053658 1102 38-39 0.2280623641773829 1102 40-41 0.2357120266074375 1102 42-43 0.2189355950917502 1102 44-45 0.11587820901887369 1102 46-47 0.1120183842526501 1102 48-49 0.35526054374604854 1102 50-51 0.2312730428693115 1102 52-53 0.32093710279931287 1102 54-55 0.5898957918099086 1102 56-57 0.5436155163369278 1102 58-59 0.43711285281252543 1102 60-61 0.6131063528622676 1102 62-63 0.49025693461515374 1102 64-65 0.5207338054385602 1102 66-67 0.46344397220359923 1102 68-69 0.4554480677486552 1102 70-71 0.2999340347387758 1102 72-73 0.13317068313667946 1102 74-75 0.254413545211186 1102 76-77 0.42558025278145806 1102 78-79 0.08506577502124557 1102 80-81 0.30495009310595833 1102 82-83 0.5226320525819332 1102 84-85 0.4719096499418427 1102 86-87 0.3057387184067615 1102 88-89 0.3152185967595429 1102 90-91 0.09796594542780923 1102 92-93 0.18085365158534827 1102 94-95 0.37903555208342254 1102 96-97 0.6040676909535492 1102 98-99 0.631437441086451 1102 100-101 0.3000816275559508 1103 1 0.05001544638540523 1103 2 0.11502170717063365 1103 3 0.11109941383754318 1103 4 0.057005598656125755 1103 5 -0.12080794696686326 1103 6 -0.10657065286596179 1103 7 0.0813466386777435 1103 8 0.2004580812902148 1103 9 0.2409808919034404 1103 10-11 0.12703435926286488 1103 12-13 0.15589460354426876 1103 14-15 0.25943065051593095 1103 16-17 0.20119140689790527 1103 18-19 0.08774087325851099 1103 20-21 0.15924832939410294 1103 22-23 0.1637667035721293 1103 24-25 0.1422051446466952 1103 26-27 0.14683345999472408 1103 28-29 0.07294813310113568 1103 30-31 0.26939427046106346 1103 32-33 0.36927697931417214 1103 34-35 0.46875913110090295 1103 36-37 0.4814412866018216 1103 38-39 0.44674409789276837 1103 40-41 0.5487130523748576 1103 42-43 0.3270677275886271 1103 44-45 0.39606908421036735 1103 46-47 0.55857386257766 1103 48-49 0.6633639068801287 1103 50-51 0.6746456056742929 1103 52-53 0.50223395829466 1103 54-55 0.6824680875784637 1103 56-57 0.3886542641027546 1103 58-59 0.3551108597916439 1103 60-61 0.07324105908673317 1103 62-63 0.40362081341678646 1103 64-65 0.3930011592600593 1103 66-67 0.38230993622992315 1103 68-69 0.30122311615566133 1103 70-71 0.342690449996379 1103 72-73 0.576781956294024 1103 74-75 0.4471830152875036 1103 76-77 0.4662726103265662 1103 78-79 0.4925934405980179 1103 80-81 0.33027810373776845 1103 82-83 0.5260218725717181 1103 84-85 0.18066706170988667 1103 86-87 0.01453713427433101 1103 88-89 0.40964981892108554 1103 90-91 0.2376854197596856 1103 92-93 0.12447223898746884 1103 94-95 0.48895513085384934 1103 96-97 0.622993410143291 1103 98-99 0.5787275577242745 1103 100-101 0.7104176306625085 1104 1 0.05561567155108449 1104 2 0.18335960110041327 1104 3 0.03615361223009472 1104 4 0.056688615171211154 1104 5 -0.07867503091708983 1104 6 0.11221900449444178 1104 7 0.06430223565320858 1104 8 0.05398114204528781 1104 9 0.1192462899752087 1104 10-11 0.24461939676110234 1104 12-13 0.1943159762177018 1104 14-15 0.2199286147714261 1104 16-17 0.14009621381062232 1104 18-19 0.195154899405928 1104 20-21 0.1561562945749415 1104 22-23 0.13914223674331083 1104 24-25 0.11641353966925294 1104 26-27 0.021989886249656365 1104 28-29 0.058214735214434654 1104 30-31 0.2104467712982796 1104 32-33 0.13007149249803973 1104 34-35 0.2956141316737302 1104 36-37 0.275221360100133 1104 38-39 0.2071023801121683 1104 40-41 0.3478464564304815 1104 42-43 0.17668164666117292 1104 44-45 0.28859364423398404 1104 46-47 0.3464929086859456 1104 48-49 0.5183544276694008 1104 50-51 0.5186631551578671 1104 52-53 0.5639906581853822 1104 54-55 0.6080275042186898 1104 56-57 0.3721817257232374 1104 58-59 0.11904711719434857 1104 60-61 0.5349920857717265 1104 62-63 0.5752314310140818 1104 64-65 0.6143550505283955 1104 66-67 0.2855702118926402 1104 68-69 0.4525127790240333 1104 70-71 0.3101609493266082 1104 72-73 0.35172437050193395 1104 74-75 0.04285488257634995 1104 76-77 0.5060901148651311 1104 78-79 0.7660244687371183 1104 80-81 0.662253585574696 1104 82-83 0.7118601190113694 1104 84-85 0.42656142331451363 1104 86-87 0.422894757740913 1104 88-89 0.5479475652814223 1104 90-91 0.5253991564083762 1104 92-93 0.7433257612220281 1104 94-95 0.657093801627255 1104 96-97 0.5928483082679392 1104 98-99 0.442979999956723 1104 100-101 0.9230854584085613 1105 1 -0.09778423216819121 1105 2 0.029610848731884687 1105 3 0.01933114652729273 1105 4 0.09034330983288896 1105 5 -0.05348169967159322 1105 6 0.005353032220590137 1105 7 0.1447893920898622 1105 8 0.18253731418801777 1105 9 0.1737755771148244 1105 10-11 0.0381883024427907 1105 12-13 0.13403312717942129 1105 14-15 0.16950627884482827 1105 16-17 -0.178230431692306 1105 18-19 0.07280928464749081 1105 20-21 0.17746460092789107 1105 22-23 0.18332266021327115 1105 24-25 0.03815897703684357 1105 26-27 -0.005062969765596392 1105 28-29 0.014835837516081085 1105 30-31 0.06681444200317799 1105 32-33 0.06521624778953594 1105 34-35 0.18864921255480027 1105 36-37 0.15025656414842103 1105 38-39 0.2275597811484289 1105 40-41 0.30085440296989674 1105 42-43 0.4329327591091001 1105 44-45 0.39338548785966765 1105 46-47 0.2736672796881834 1105 48-49 0.29022243145153226 1105 50-51 0.48741510311520386 1105 52-53 0.6520803870146992 1105 54-55 0.7310462203805557 1105 56-57 0.6773133306598424 1105 58-59 0.35401076408592047 1105 60-61 0.43072144981575633 1105 62-63 0.589602005161133 1105 64-65 0.49998676699141953 1105 66-67 0.5637815157905095 1105 68-69 0.3892213450795978 1105 70-71 0.34337573420077305 1105 72-73 0.21834158252155333 1105 74-75 0.2696667739218235 1105 76-77 0.19432043458172643 1105 78-79 0.36520957481489447 1105 80-81 0.49668415868534055 1105 82-83 0.5659230980211163 1105 84-85 0.18173594902679824 1105 86-87 0.47098517750835356 1105 88-89 0.6925047753333686 1105 90-91 0.4962778043926761 1105 92-93 0.4381584828498184 1105 94-95 0.7631591869041152 1105 96-97 0.6598843264215866 1105 98-99 0.6578717066715107 1105 100-101 0.8316784755776219 1106 1 -0.06181519028353932 1106 2 0.04002562053584313 1106 3 0.10022025855521122 1106 4 0.1436626242139596 1106 5 0.06984142262557924 1106 6 0.12995314039451245 1106 7 0.08910043819960123 1106 8 0.04068516320050719 1106 9 0.17219905134105318 1106 10-11 0.11818970918886862 1106 12-13 0.1591320238539211 1106 14-15 0.22313240939570989 1106 16-17 0.19678442403133545 1106 18-19 0.016364204741890376 1106 20-21 0.06454533602547485 1106 22-23 0.03629950468779697 1106 24-25 0.01932191946454509 1106 26-27 0.19566514259945933 1106 28-29 0.16744656419744786 1106 30-31 0.23494909670765196 1106 32-33 0.05546422316663069 1106 34-35 0.12251213727690669 1106 36-37 0.18372135547704715 1106 38-39 0.10499702935906896 1106 40-41 0.3590967222575969 1106 42-43 0.306939244693595 1106 44-45 -0.15067295031262518 1106 46-47 -0.19322628541870301 1106 48-49 -0.23027482903175667 1106 50-51 0.043981454234177875 1106 52-53 0.16888372633882653 1106 54-55 0.3914546860814241 1106 56-57 0.22912281843449023 1106 58-59 0.6223688003654146 1106 60-61 0.2770010915320924 1106 62-63 0.1816547387355527 1106 64-65 -0.15729714589704002 1106 66-67 0.08041871355232644 1106 68-69 -0.047635159544476835 1106 70-71 -0.21353927165662512 1106 72-73 0.10753789393632829 1106 74-75 -0.0023287924026149653 1106 76-77 -0.008958011400437726 1106 78-79 -0.1870189050019384 1106 80-81 -0.16562220150471774 1106 82-83 0.05157792619193913 1106 84-85 0.07596319672810026 1106 86-87 0.12806474840229143 1106 88-89 0.3895862623124806 1106 90-91 0.27816606994752746 1106 92-93 0.24764140665026346 1106 94-95 0.21777404165758085 1106 96-97 0.3790377805895595 1106 98-99 0.30286599479736154 1106 100-101 0.8030887870263506 1107 1 0.2601418156336486 1107 2 0.37670725633534374 1107 3 0.2671250219852581 1107 4 0.18135131322969045 1107 5 0.11007123404866803 1107 6 0.23836083892770432 1107 7 0.3495441309843059 1107 8 0.10027341595463213 1107 9 0.22996794586739355 1107 10-11 0.17186664925040418 1107 12-13 0.14134576855013137 1107 14-15 0.39737439685239195 1107 16-17 0.2887986799415785 1107 18-19 0.3016249473902519 1107 20-21 0.3652540742834631 1107 22-23 0.055697984712217874 1107 24-25 0.28457719549663807 1107 26-27 0.28091446087397287 1107 28-29 0.43236978599900056 1107 30-31 0.38523513313325 1107 32-33 0.3206228724853375 1107 34-35 0.35643526725638 1107 36-37 0.4733505864992438 1107 38-39 0.36941362822688717 1107 40-41 0.5813440353943449 1107 42-43 0.43123162751901845 1107 44-45 0.2538673307483208 1107 46-47 0.17853191503955657 1107 48-49 0.07077450504468885 1107 50-51 -0.1777277388633678 1107 52-53 -0.12077112944712809 1107 54-55 0.01654090273452624 1107 56-57 -0.10846155643871924 1107 58-59 -0.012848273414682865 1107 60-61 -0.09919606540037051 1107 62-63 0.08579036206860025 1107 64-65 0.09407858360794563 1107 66-67 0.2021507553975681 1107 68-69 0.024191053307625054 1107 70-71 -0.02345637554582325 1107 72-73 0.18501009640612764 1107 74-75 0.12180023255377037 1107 76-77 -0.2628535093429818 1107 78-79 -0.18184804121250409 1107 80-81 -0.04718934799508645 1107 82-83 0.5561167124750028 1107 84-85 0.28176475001586354 1107 86-87 0.7469962171069788 1107 88-89 0.9125860893453215 1107 90-91 0.9655407488372063 1107 92-93 0.9295521385148717 1107 94-95 1.1813511092023248 1107 96-97 1.0936553203132782 1107 98-99 1.033227816461821 1107 100-101 1.3269657283691316 1108 1 -0.14160125179665428 1108 2 0.03176508893815111 1108 3 0.001865403752617567 1108 4 0.01715003038383145 1108 5 -0.27874256911388784 1108 6 -0.15143861995725416 1108 7 -0.12775591904518535 1108 8 -0.10691963069226773 1108 9 -0.2164868048189561 1108 10-11 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6554.0 22 6999.0 23 7958.0 24 9042.0 25 10838.0 26 13141.0 27 16395.0 28 20244.0 29 24552.0 30 30618.0 31 37947.0 32 47497.0 33 60386.0 34 79846.0 35 111307.0 36 166629.0 37 232968.0 38 139897.0 39 6919.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.01221161825348 20.40522605521736 15.602833649266218 21.979728677262944 2 15.331378876014002 21.447027011765908 40.128761032529056 23.09283307969104 3 18.89854349170383 25.969161330629113 30.455804312605128 24.676490865061922 4 12.888578032534276 18.074991666985415 37.76576852251553 31.270661777964776 5 13.367151782299047 38.12562612702865 34.20648075627038 14.300741334401923 6 30.48016378244184 37.58289755765262 17.758701397578598 14.178237262326942 7 27.12465324515181 33.882707155854355 21.37676473856096 17.615874860432882 8 25.885804003854133 35.38380134821801 20.02819564278962 18.702199005138237 9 26.21231811926877 17.122038248116418 20.8828267796922 35.782816852922615 10-11 24.2853187765131 27.61624783022872 27.540886414180875 20.557546979077305 12-13 25.517871390110354 24.989434913603095 28.216431259540453 21.276262436746098 14-15 22.656825627261526 26.127176369119127 26.8512286575342 24.364769346085154 16-17 21.36270743742043 29.42505842217329 27.378961824775374 21.833272315630914 18-19 21.32873421900436 27.70001024555172 30.161720341973748 20.80953519347017 20-21 22.332854446475856 26.800891594738772 30.33348284317632 20.532771115609055 22-23 21.90729371864262 26.631130199894905 30.28609496852081 21.175481112941664 24-25 21.76487999016425 26.811895795671358 30.424440022403658 20.998784191760738 26-27 21.650040822757287 27.008682292394763 30.256106451543864 21.085170433304086 28-29 21.44811598479291 27.170172684887305 30.220378938663924 21.16133239165586 30-31 21.717485177017444 26.94105823869134 30.188339876042935 21.153116708248277 32-33 21.63984694567555 27.018468791935458 30.08164991942472 21.260034342964268 34-35 21.766881412550703 27.072962600701977 29.93523927135756 21.224916715389757 36-37 21.852290181138944 26.87471909779791 30.071460154122242 21.201530566940903 38-39 21.752509384718586 27.129407789305045 29.91797735462737 21.200105471348998 40-41 21.782644847122636 27.005829493905942 29.846671833462807 21.36485382550861 42-43 21.63674067112187 27.171638243345882 29.692534529644586 21.49908655588766 44-45 21.847315640236005 27.26753618846004 29.47392914534262 21.41121902596134 46-47 22.04987978580015 27.448908965065026 29.04457032530691 21.456640923827912 48-49 22.196145502631044 27.65855040153291 28.602958721068955 21.542345374767084 50-51 22.1007612787005 27.813356939439227 28.468125837301837 21.617755944558436 52-53 22.25059606315993 27.83191474247704 28.31700724382945 21.600481950533588 54-55 22.14519116755097 27.745705537675242 28.317359445267122 21.791743849506666 56-57 22.17159666314524 28.127390623292413 28.157808458708246 21.543204254854103 58-59 22.21969752509354 27.79785645624505 28.27784742197813 21.704598596683276 60-61 22.153694551670718 27.65147668680451 28.639440882209765 21.555387879315 62-63 21.912823006770232 27.645026328683237 28.844479537212603 21.59767112733393 64-65 21.939461332080192 27.706135102300276 28.659149650013386 21.69525391560614 66-67 21.96177377550221 27.86350613182374 28.311713031013387 21.863007061660667 68-69 21.981247504076578 27.77110680343758 28.465935455021924 21.78171023746392 70-71 22.046215410844127 27.634850350445163 28.44730390371137 21.871630334999345 72-73 22.009817537692335 27.688507196947278 28.25082989157905 22.050845373781346 74-75 21.988182925579753 28.03242295563474 27.88355969444097 22.09583442434454 76-77 22.0611625891759 27.902067364546205 27.98512441025627 22.05164563602162 78-79 22.046459230008434 27.88409872882359 27.91233090293779 22.15711113823019 80-81 21.99107109517517 28.06671542166853 27.87791881108226 22.064294672074038 82-83 22.084415945153133 28.009934073379906 27.849602791886262 22.0560471895807 84-85 22.01747631262463 27.983138441193788 27.704950954029623 22.294434292151962 86-87 21.883799802556563 27.92290052420868 27.76844292901143 22.424856744223323 88-89 21.96822838043065 27.80533564664604 27.671454950739644 22.554981022183668 90-91 21.921592770928523 27.859966794503883 27.86770806485545 22.35073236971214 92-93 21.78172601776617 27.873478427965416 27.801577717427932 22.543217836840483 94-95 21.878261487265515 27.889871243540775 27.60718624606345 22.624681023130265 96-97 22.02698456788718 27.942825137632227 27.437058017276318 22.59313227720428 98-99 21.755567030683217 28.004912447508122 27.35770614019509 22.881814381613566 100-101 21.65010186601045 28.034327524951934 27.274499071502927 23.041071537534688 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 275.0 1 199.5 2 138.5 3 169.0 4 159.5 5 152.0 6 261.0 7 555.0 8 943.0 9 1229.5 10 1237.0 11 1139.0 12 1215.5 13 1322.0 14 1447.5 15 1604.5 16 1738.0 17 1853.5 18 1899.5 19 2074.5 20 2327.0 21 2619.0 22 3165.0 23 3885.5 24 4600.0 25 5511.5 26 7116.5 27 9135.5 28 10793.5 29 12539.0 30 14683.5 31 17010.5 32 19260.0 33 21505.0 34 24245.0 35 26789.0 36 29911.0 37 32275.0 38 33609.0 39 34739.5 40 35211.0 41 36158.0 42 36854.0 43 37895.5 44 39725.5 45 40849.5 46 40673.0 47 40453.5 48 41523.0 49 40907.5 50 38756.0 51 37335.0 52 36513.0 53 35817.0 54 34340.0 55 31464.5 56 27782.0 57 24815.0 58 22706.5 59 20168.5 60 16572.0 61 13079.5 62 9931.5 63 7212.5 64 5096.0 65 3612.0 66 2495.5 67 1789.5 68 1229.0 69 742.5 70 474.0 71 263.0 72 153.5 73 95.0 74 64.5 75 37.5 76 22.0 77 9.5 78 5.5 79 4.5 80 3.5 81 2.0 82 1.0 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02140173144560955 2 0.0035517767079947765 3 4.5535598820445854E-4 4 0.0 5 0.0020035663480996173 6 0.0 7 3.642847905635668E-4 8 0.0 9 9.10711976408917E-5 10-11 0.0 12-13 0.007832122997116686 14-15 0.005008915870249043 16-17 0.0 18-19 4.098203893840127E-4 20-21 8.651763775884712E-4 22-23 0.0013660679646133754 24-25 6.374983834862419E-4 26-27 2.2767799410222927E-4 28-29 1.3660679646133756E-4 30-31 4.553559882044585E-5 32-33 4.553559882044585E-5 34-35 0.0 36-37 0.0015937459587156049 38-39 0.010336580932241208 40-41 4.5535598820445854E-4 42-43 0.0 44-45 5.008915870249043E-4 46-47 1.821423952817834E-4 48-49 0.0 50-51 0.0019124951504587257 52-53 3.642847905635668E-4 54-55 0.0 56-57 1.821423952817834E-4 58-59 4.553559882044585E-5 60-61 0.00241338673748363 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.3660679646133756E-4 70-71 9.107119764089171E-4 72-73 6.374983834862419E-4 74-75 0.004963380271428597 76-77 1.3660679646133756E-4 78-79 3.642847905635668E-4 80-81 0.0031874919174312098 82-83 2.732135929226751E-4 84-85 0.0037794547020970058 86-87 5.464271858453502E-4 88-89 0.004508024283224139 90-91 0.0028232071268676426 92-93 0.0 94-95 0.0 96-97 6.374983834862419E-4 98-99 0.0013660679646133754 100-101 0.0028232071268676426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1098042.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.01483374934158 #Duplication Level Percentage of deduplicated Percentage of total 1 81.19127055239194 51.974456863091824 2 11.252875421225149 14.407018985835599 3 3.2956543536205225 6.329122966269346 4 1.4031059065298195 3.592783653569023 5 0.7073771170772694 2.2641314273894966 6 0.4800075933393066 1.8436563771622358 7 0.3073743547924654 1.3773562754595565 8 0.22398822057589926 1.1470854961581634 9 0.16304104042336764 0.9393340587319401 >10 0.8991327261069663 10.357142514005712 >50 0.05422039954576287 2.3394118793286527 >100 0.02109703092336314 2.1257035567321925 >500 2.8509448274904566E-4 0.12072557518456946 >1k 4.2764172412356854E-4 0.7172358435001632 >5k 1.4254724137452283E-4 0.4648345275814035 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5094 0.4639166807827023 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4211 0.38350081326579494 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2583 0.23523690350642323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.821423952817834E-4 0.0 0.0 0.0 0.0 7 1.821423952817834E-4 0.0 0.0 0.0 0.0 8 1.821423952817834E-4 0.0 0.0 0.0 0.0 9 1.821423952817834E-4 0.0 0.0 0.0 0.0 10-11 1.821423952817834E-4 0.0 0.0 0.0 0.0 12-13 2.732135929226751E-4 0.0 0.0 0.0 0.0 14-15 2.732135929226751E-4 0.0 0.0 0.0 0.0 16-17 2.732135929226751E-4 0.0 0.0 4.553559882044585E-5 4.553559882044585E-5 18-19 4.0982038938401264E-4 0.0 0.0 9.10711976408917E-5 1.821423952817834E-4 20-21 5.464271858453502E-4 0.0 0.0 2.2767799410222927E-4 1.821423952817834E-4 22-23 5.464271858453502E-4 0.0 0.0 4.5535598820445854E-4 1.821423952817834E-4 24-25 5.464271858453502E-4 0.0 0.0 9.562475752293628E-4 1.821423952817834E-4 26-27 5.464271858453502E-4 0.0 0.0 0.0013660679646133754 1.821423952817834E-4 28-29 5.464271858453502E-4 0.0 0.0 0.00241338673748363 1.821423952817834E-4 30-31 5.464271858453502E-4 0.0 0.0 0.005008915870249043 1.821423952817834E-4 32-33 5.464271858453502E-4 0.0 0.0 0.00801426539239847 1.821423952817834E-4 34-35 5.464271858453502E-4 0.0 0.0 0.010564258926343436 1.821423952817834E-4 36-37 5.464271858453502E-4 0.0 0.0 0.013114252460288404 1.821423952817834E-4 38-39 5.464271858453502E-4 0.0 0.0 0.016848171563564964 1.821423952817834E-4 40-41 5.91962784665796E-4 0.0 0.0 0.0208553042597642 1.821423952817834E-4 42-43 7.741051799475794E-4 0.0 0.0 0.029370461239187573 1.821423952817834E-4 44-45 0.0010928543716907004 0.0 0.0 0.04307667648414177 2.2767799410222927E-4 46-47 0.0012749967669724838 0.0 0.0 0.05582664415386661 2.732135929226751E-4 48-49 0.0014571391622542672 0.0 0.0 0.08665424455530846 2.732135929226751E-4 50-51 0.0014571391622542672 0.0 0.0 0.11443095983578042 2.732135929226751E-4 52-53 0.0014571391622542672 0.0 0.0 0.14534963143486312 2.732135929226751E-4 54-55 0.0016392815575360506 9.10711976408917E-5 0.0 0.2060941202613379 2.732135929226751E-4 56-57 0.0017303527551769421 9.10711976408917E-5 0.0 0.26683860908781265 3.1874919174312095E-4 58-59 0.0017303527551769421 9.10711976408917E-5 0.0 0.350214290528049 3.642847905635668E-4 60-61 0.0017303527551769421 1.821423952817834E-4 0.0 0.4422872713429905 3.642847905635668E-4 62-63 0.0017303527551769421 1.821423952817834E-4 0.0 0.5590860823174342 3.642847905635668E-4 64-65 0.0017303527551769421 2.732135929226751E-4 0.0 0.6489733543889942 3.642847905635668E-4 66-67 0.001821423952817834 2.732135929226751E-4 0.0 0.7726025051865046 3.642847905635668E-4 68-69 0.001821423952817834 2.732135929226751E-4 0.0 0.9391717256716956 3.642847905635668E-4 70-71 0.001821423952817834 2.732135929226751E-4 0.0 1.0498687664041997 3.642847905635668E-4 72-73 0.001821423952817834 2.732135929226751E-4 0.0 1.2072853315264807 4.0982038938401264E-4 74-75 0.001821423952817834 2.732135929226751E-4 0.0 1.4056383999883428 4.553559882044585E-4 76-77 0.001821423952817834 2.732135929226751E-4 0.0 1.56464871106934 4.553559882044585E-4 78-79 0.001821423952817834 2.732135929226751E-4 0.0 1.7067197793891307 4.553559882044585E-4 80-81 0.0019124951504587257 2.732135929226751E-4 0.0 1.9007469659630507 4.553559882044585E-4 82-83 0.002094637545740509 2.732135929226751E-4 0.0 2.1869837401483734 4.553559882044585E-4 84-85 0.0021857087433814005 2.732135929226751E-4 0.0 2.5216248558798298 4.553559882044585E-4 86-87 0.0022767799410222922 2.732135929226751E-4 0.0 2.897794437735533 4.553559882044585E-4 88-89 0.0022767799410222922 2.732135929226751E-4 0.0 3.292451472712337 5.008915870249043E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3345 0.0 41.054096 1 GTATCAA 5660 0.0 37.107407 1 ATCAACG 7055 0.0 29.286602 3 TATCAAC 7160 0.0 28.924776 2 TCAACGC 7145 0.0 28.917702 4 CAACGCA 7275 0.0 28.40096 5 AACGCAG 7455 0.0 27.717745 6 GTGTACG 135 1.3388015E-5 24.63877 1 ACGCAGA 8400 0.0 24.429848 7 CGCAGAG 8395 0.0 24.276857 8 TACATGG 4790 0.0 23.502245 2 GTACATG 4780 0.0 23.460587 1 TGAACCG 170 3.1498348E-6 22.352074 5 GCAGAGT 8855 0.0 22.050028 9 TTGGACG 130 2.942165E-4 21.922226 4 GGCGCGC 220 5.630136E-8 21.594006 8 ACATGGG 4865 0.0 21.088596 3 GGGCGCG 205 6.82523E-7 20.85475 7 AGAGTAC 7635 0.0 20.40802 10-11 GAGTACT 4900 0.0 20.40668 12-13 >>END_MODULE