FastQCFastQC Report
Thu 26 May 2016
SRR937131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318142
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123190.5314169710052274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92090.3972578038791411No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56470.2436002626241188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73150.050.742561
GTATCAA133550.043.3450051
TATCAAC172750.033.5650142
ATCAACG173150.033.5380253
TCAACGC173900.033.421434
CAACGCA177650.032.769445
AACGCAG183000.031.9146126
ACGCAGA205600.028.1747467
CGCAGAG206300.027.8239618
GCAGAGT214150.025.6724039
GTACATG152750.025.2956031
TACATGG156950.024.3769662
GAGTACT104000.023.77761712-13
ACATGGG159750.022.8756943
GTACTTT112450.021.84463714-15
AGTACTT109850.021.64636412-13
CAGAGTA208900.021.19736310-11
AGAGTAC197700.021.06442610-11
TACACCG2601.48393156E-820.1056235
CATGGGA95650.020.0721824
Produced by FastQC (version 0.11.5)