##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937131_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2318142 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56978045348387 31.0 31.0 34.0 30.0 34.0 2 31.65184876508859 33.0 31.0 34.0 30.0 34.0 3 31.651955747318326 33.0 31.0 34.0 28.0 34.0 4 35.24469424219914 37.0 35.0 37.0 33.0 37.0 5 35.27503146916798 37.0 35.0 37.0 33.0 37.0 6 35.3337901647095 37.0 35.0 37.0 33.0 37.0 7 35.26975224123458 37.0 35.0 37.0 32.0 37.0 8 35.23506023358362 37.0 35.0 37.0 32.0 37.0 9 36.80856867266975 39.0 37.0 39.0 33.0 39.0 10-11 36.66696302469823 39.0 37.0 39.0 32.0 39.0 12-13 36.54694859072481 39.0 37.0 39.0 32.0 39.0 14-15 37.6266423713474 40.0 37.0 41.0 32.0 41.0 16-17 37.56702199433857 40.0 37.0 41.0 32.0 41.0 18-19 37.545457957277854 40.0 37.0 41.0 32.0 41.0 20-21 37.496971497000615 40.0 37.0 41.0 32.0 41.0 22-23 37.35474552464862 40.0 37.0 41.0 31.5 41.0 24-25 37.173469097233905 39.5 36.5 41.0 31.0 41.0 26-27 37.128915959419224 39.0 36.5 41.0 31.0 41.0 28-29 37.189812358345606 40.0 37.0 41.0 31.0 41.0 30-31 37.033388593106025 39.0 36.0 41.0 31.0 41.0 32-33 36.832908855454065 39.0 36.0 41.0 30.0 41.0 34-35 36.63506959452872 39.0 35.0 41.0 30.0 41.0 36-37 36.47191630193491 39.0 35.0 41.0 30.0 41.0 38-39 36.30089873700575 39.0 35.0 41.0 30.0 41.0 40-41 36.07023512796024 39.0 35.0 40.5 29.5 41.0 42-43 35.830963978910695 39.0 35.0 40.0 28.5 41.0 44-45 35.54666603685193 38.5 35.0 40.0 27.0 41.0 46-47 35.141942987099156 38.0 34.5 40.0 25.5 41.0 48-49 34.75639693340615 38.0 34.0 40.0 24.0 41.0 50-51 34.088289241987766 37.5 33.5 39.5 23.5 40.5 52-53 34.37577163090096 38.0 34.0 40.0 24.0 40.5 54-55 34.67461829344363 38.0 34.0 40.0 24.0 41.0 56-57 34.554964924495565 38.0 34.0 40.0 23.5 41.0 58-59 34.30108013227835 38.0 34.0 40.0 23.0 41.0 60-61 34.01235450632446 38.0 34.0 40.0 22.5 41.0 62-63 33.69454934167105 37.0 33.0 40.0 22.0 41.0 64-65 33.3720768615555 37.0 33.0 40.0 21.5 41.0 66-67 33.003603963864165 36.0 32.5 39.0 20.0 41.0 68-69 32.56750319868239 36.0 32.0 39.0 18.0 41.0 70-71 32.220001190608684 35.0 32.0 38.5 18.0 40.0 72-73 31.851534763616723 35.0 31.0 37.5 16.5 40.0 74-75 31.42953775049156 35.0 31.0 37.0 13.0 39.0 76-77 30.99978085898103 35.0 31.0 36.5 9.5 39.0 78-79 30.58969165823319 35.0 30.5 36.0 7.0 39.0 80-81 30.186885229636495 34.5 30.0 36.0 6.5 37.0 82-83 29.793909950296403 34.0 30.0 35.0 2.0 37.0 84-85 29.45601844063047 34.0 29.5 35.0 2.0 36.5 86-87 29.130846600423958 34.0 29.0 35.0 2.0 36.0 88-89 28.77583814969057 34.0 29.0 35.0 2.0 36.0 90-91 28.45192119378364 34.0 29.0 35.0 2.0 35.5 92-93 28.10172370803859 34.0 29.0 35.0 2.0 35.0 94-95 27.709569776139684 34.0 27.0 35.0 2.0 35.0 96-97 27.270951908899455 34.0 27.0 35.0 2.0 35.0 98-99 26.670574537711666 33.5 25.5 35.0 2.0 35.0 100-101 24.54240529700079 31.0 20.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5639032938878934 1101 2 0.44345217056769926 1101 3 0.6293614255029389 1101 4 0.4854826392805194 1101 5 0.449092832235948 1101 6 0.44497770943851833 1101 7 0.5463165517281823 1101 8 0.5085917163642577 1101 9 0.6229239946267597 1101 10-11 0.5833752683982709 1101 12-13 0.6611473487829045 1101 14-15 0.9101773387517014 1101 16-17 1.0028173474159559 1101 18-19 1.0086909594909486 1101 20-21 0.8874570024415789 1101 22-23 0.8384214362379865 1101 24-25 0.8833962876393784 1101 26-27 0.9463569382594059 1101 28-29 1.0489422496960472 1101 30-31 0.9896827541973039 1101 32-33 1.0550427442139778 1101 34-35 0.9629433758768613 1101 36-37 0.892064949959348 1101 38-39 0.942741543113442 1101 40-41 1.043834587872773 1101 42-43 1.0184744852947034 1101 44-45 1.1028347915780046 1101 46-47 1.0561866040081966 1101 48-49 1.0059587695351837 1101 50-51 0.9513200982074679 1101 52-53 1.0456189061974754 1101 54-55 1.1339860022455284 1101 56-57 1.1198287746601778 1101 58-59 0.9826465528433133 1101 60-61 1.05015368414481 1101 62-63 1.123438157590975 1101 64-65 1.0011993030716653 1101 66-67 0.9691690021213688 1101 68-69 0.9936670774777454 1101 70-71 0.955888440425916 1101 72-73 1.0494280861180236 1101 74-75 0.8941160468045055 1101 76-77 0.935941465692089 1101 78-79 0.9585058355660507 1101 80-81 0.9786573247648747 1101 82-83 0.8141010728079543 1101 84-85 1.0283718946636462 1101 86-87 1.1581827794309376 1101 88-89 1.0539849129064294 1101 90-91 1.006357005506537 1101 92-93 1.074564737648938 1101 94-95 1.1251569722111974 1101 96-97 1.0204525118616807 1101 98-99 1.150847384784317 1101 100-101 1.541827026964409 1102 1 0.4177987592105019 1102 2 0.42824443475086227 1102 3 0.2559558894833671 1102 4 0.37503630391626075 1102 5 0.35556773168027433 1102 6 0.45963582013729365 1102 7 0.4655519179323946 1102 8 0.3615476534178086 1102 9 0.5297507578902767 1102 10-11 0.6223016082580912 1102 12-13 0.5257231129736226 1102 14-15 0.6091413072914449 1102 16-17 0.5403556839300379 1102 18-19 0.5258274623363306 1102 20-21 0.5598447702028153 1102 22-23 0.6143870625145169 1102 24-25 0.6908496192148377 1102 26-27 0.5858691054422636 1102 28-29 0.7216540001753202 1102 30-31 0.6867913327150319 1102 32-33 0.6806437548498536 1102 34-35 0.641411168882378 1102 36-37 0.5399572251652671 1102 38-39 0.5692374506432856 1102 40-41 0.647942079243677 1102 42-43 0.7093760606849813 1102 44-45 0.6550897026502653 1102 46-47 0.44402017760889834 1102 48-49 0.5463441122890131 1102 50-51 0.25054050803804273 1102 52-53 0.41236464136159867 1102 54-55 0.5547781713303621 1102 56-57 0.6625979515625247 1102 58-59 0.45245834460391166 1102 60-61 0.4937851219234801 1102 62-63 0.491317075804254 1102 64-65 0.47444490099160674 1102 66-67 0.23080892958727617 1102 68-69 0.33830201876533295 1102 70-71 0.3890024352257626 1102 72-73 0.5248634311412523 1102 74-75 0.5210840959271152 1102 76-77 0.49726162502501836 1102 78-79 0.5666598689239528 1102 80-81 0.6057657519898001 1102 82-83 0.5871172460079492 1102 84-85 0.5119276644305941 1102 86-87 0.6705597189505639 1102 88-89 0.7821914016383573 1102 90-91 0.6511447525190448 1102 92-93 0.6589197656489141 1102 94-95 0.7569486679657054 1102 96-97 0.5560232855514293 1102 98-99 0.5355698381483158 1102 100-101 0.7721684731938687 1103 1 0.30402402950386076 1103 2 0.44356523055173014 1103 3 0.3742555743894549 1103 4 0.4218267565309901 1103 5 0.38166966634894806 1103 6 0.3669123835871986 1103 7 0.2903259284461228 1103 8 0.32708480310926547 1103 9 0.37963135597269115 1103 10-11 0.35778066623457505 1103 12-13 0.27071180853251064 1103 14-15 0.30284399850338417 1103 16-17 0.4987191823068855 1103 18-19 0.5313594862882383 1103 20-21 0.37751446595640203 1103 22-23 0.19033204624327738 1103 24-25 0.575602928098256 1103 26-27 0.5236064689048803 1103 28-29 0.510949946072941 1103 30-31 0.6877306542896235 1103 32-33 0.5996060111288841 1103 34-35 0.5180326310293921 1103 36-37 0.6933817357366152 1103 38-39 0.7439836470663863 1103 40-41 0.811864024958524 1103 42-43 0.8246082487400272 1103 44-45 0.8927273327308072 1103 46-47 0.8068945254678042 1103 48-49 0.7704978685819626 1103 50-51 0.8073937012943233 1103 52-53 0.7956743989419266 1103 54-55 0.847787099653452 1103 56-57 0.9694729987471433 1103 58-59 0.9844264696560572 1103 60-61 0.9190476670717089 1103 62-63 0.9468057892436264 1103 64-65 1.0065515004806187 1103 66-67 0.9249075742463759 1103 68-69 0.962911131456103 1103 70-71 0.9203606370050679 1103 72-73 0.8734045820883161 1103 74-75 0.7427920002943296 1103 76-77 0.786592338535911 1103 78-79 0.9098096557488127 1103 80-81 0.9371378608144134 1103 82-83 0.7515597125615017 1103 84-85 0.9415558297734385 1103 86-87 0.9523736744718576 1103 88-89 0.9913263436857136 1103 90-91 0.7840535898503198 1103 92-93 0.627397460734958 1103 94-95 0.802090142350913 1103 96-97 0.758723835078392 1103 98-99 1.0831861705823265 1103 100-101 1.2475754529412377 1104 1 0.4084684076526486 1104 2 0.5337292750178797 1104 3 0.503526268268832 1104 4 0.4386089641018671 1104 5 0.33776138805136924 1104 6 0.34889583370447497 1104 7 0.3481934622600136 1104 8 0.3660195395902335 1104 9 0.3857296036541058 1104 10-11 0.45376103608977303 1104 12-13 0.4974282342351657 1104 14-15 0.66424749419064 1104 16-17 0.6134442148435539 1104 18-19 0.5476790951769956 1104 20-21 0.6923901553794636 1104 22-23 0.7004599927227915 1104 24-25 0.6609618010057474 1104 26-27 0.6843555021277083 1104 28-29 0.7955885373564655 1104 30-31 0.8231284966145154 1104 32-33 0.7407140850700955 1104 34-35 0.6971174277358188 1104 36-37 0.7279759503630103 1104 38-39 0.62864554541774 1104 40-41 0.6734168723363894 1104 42-43 0.7491366069155845 1104 44-45 0.5503176056658887 1104 46-47 0.7510944217979727 1104 48-49 0.4057217469526293 1104 50-51 0.39626280507230405 1104 52-53 0.6146454198660791 1104 54-55 0.6450420864336834 1104 56-57 0.717712103159073 1104 58-59 0.7492309326798647 1104 60-61 0.6647862027911913 1104 62-63 0.6149291787252409 1104 64-65 0.6047751289995205 1104 66-67 0.43824947215022547 1104 68-69 0.659669398006919 1104 70-71 0.5037904305230896 1104 72-73 0.5039547407628326 1104 74-75 0.3947632188624155 1104 76-77 0.5487862235071397 1104 78-79 0.6230547827070758 1104 80-81 0.6236307624138391 1104 82-83 0.5086008709787109 1104 84-85 0.5711681120388121 1104 86-87 0.5399994860239588 1104 88-89 0.46584314155563433 1104 90-91 0.3420259122411444 1104 92-93 0.2812474154260194 1104 94-95 0.3933048694344734 1104 96-97 0.442521880268 1104 98-99 0.4510729467114132 1104 100-101 0.5063245532884189 1105 1 0.23739869480477793 1105 2 0.1659060575504867 1105 3 0.2898718699669338 1105 4 0.18515811311804242 1105 5 0.23813811927543327 1105 6 0.28701948729449356 1105 7 0.3527318083677713 1105 8 0.31092400378495455 1105 9 0.36322975301116855 1105 10-11 0.344422354016487 1105 12-13 0.25807950131019197 1105 14-15 0.3069511381515255 1105 16-17 0.4055254791422982 1105 18-19 0.49114797443674263 1105 20-21 0.5440975701668691 1105 22-23 0.39217401436375354 1105 24-25 0.4789858597926866 1105 26-27 0.3496779552383771 1105 28-29 0.38291339423726356 1105 30-31 0.24909694537449667 1105 32-33 0.2377392438278605 1105 34-35 0.1768354416031812 1105 36-37 0.14316609068836783 1105 38-39 0.20663751879352787 1105 40-41 0.42077465805644465 1105 42-43 0.4182799650949036 1105 44-45 0.2857844606981672 1105 46-47 0.3283907426942534 1105 48-49 0.32296071473718513 1105 50-51 0.1523161589290467 1105 52-53 0.15048777612308584 1105 54-55 0.2880210745308034 1105 56-57 0.48242517945769237 1105 58-59 0.41017898102234795 1105 60-61 0.2910542442456858 1105 62-63 0.2062573320042418 1105 64-65 0.5508342111388842 1105 66-67 0.6700052434828123 1105 68-69 0.6148824866055307 1105 70-71 0.6439674801412636 1105 72-73 0.49386031195694713 1105 74-75 0.6892491318557745 1105 76-77 0.3915791302767033 1105 78-79 0.4676824970520279 1105 80-81 0.5441598026980721 1105 82-83 0.6246418194136254 1105 84-85 0.6664313846101777 1105 86-87 0.7529810746910961 1105 88-89 0.6598493548094027 1105 90-91 0.6674974412708856 1105 92-93 0.6396982136955849 1105 94-95 0.6616935351663429 1105 96-97 0.6914533622766257 1105 98-99 0.6937806320986688 1105 100-101 0.9797110577357486 1106 1 0.4078192508621896 1106 2 0.4045088880232335 1106 3 0.47104220018875154 1106 4 0.25930244715397777 1106 5 0.3473020257293129 1106 6 0.35668725804627854 1106 7 0.3310114430159672 1106 8 0.2970827912987559 1106 9 0.5529216128927317 1106 10-11 0.5974987366321614 1106 12-13 0.4512090807541398 1106 14-15 0.7012098955539088 1106 16-17 0.5364498994976117 1106 18-19 0.5296504335354086 1106 20-21 0.6723571305402487 1106 22-23 0.6439254948415183 1106 24-25 0.6615664078203949 1106 26-27 0.6518459769185938 1106 28-29 0.6517029154658047 1106 30-31 0.52495762990727 1106 32-33 0.6252982120101365 1106 34-35 0.5273122056300892 1106 36-37 0.4543825917422595 1106 38-39 0.5535109504908036 1106 40-41 0.48251309025557987 1106 42-43 0.4369992263615856 1106 44-45 0.6740159170868054 1106 46-47 0.2355823516473876 1106 48-49 0.4472857172464657 1106 50-51 0.4908938965582834 1106 52-53 0.49435508516655347 1106 54-55 0.43663594956844065 1106 56-57 0.5969087687380323 1106 58-59 0.6342329808216078 1106 60-61 0.5730024526166559 1106 62-63 0.6015499145596905 1106 64-65 0.5191720611840438 1106 66-67 0.7042770491638279 1106 68-69 0.5883593510038025 1106 70-71 0.5361636058062231 1106 72-73 0.5109134084138454 1106 74-75 0.49642568510278906 1106 76-77 0.6506878324748371 1106 78-79 0.6529831093174074 1106 80-81 0.7430908475640763 1106 82-83 0.754467173222718 1106 84-85 0.7935818412999325 1106 86-87 0.7982435458343247 1106 88-89 0.9363423789989085 1106 90-91 0.8269223132973842 1106 92-93 0.8490979928785585 1106 94-95 0.8670483479737285 1106 96-97 0.869291362878851 1106 98-99 0.950786554009742 1106 100-101 1.0077096525365086 1107 1 0.18296372124783034 1107 2 0.24253978564382095 1107 3 0.21982250401351777 1107 4 0.2123496178157751 1107 5 0.19611838965669648 1107 6 0.2153759949675873 1107 7 0.3182183856896046 1107 8 0.31746981186957157 1107 9 0.37514714675702976 1107 10-11 0.3923936563892383 1107 12-13 0.42483626712783007 1107 14-15 0.30962100648104496 1107 16-17 0.45278227429454887 1107 18-19 0.5041691875484773 1107 20-21 0.4971362164166493 1107 22-23 0.5538424830134332 1107 24-25 0.4143835113478431 1107 26-27 0.4196447652255486 1107 28-29 0.3292398632724911 1107 30-31 0.5036815254286466 1107 32-33 0.5663488672553214 1107 34-35 0.6256758864834566 1107 36-37 0.4843870808570756 1107 38-39 0.48410895945799837 1107 40-41 0.6290453410499168 1107 42-43 0.6041985154624285 1107 44-45 0.659264299441638 1107 46-47 0.8652852973530045 1107 48-49 0.8270587593760794 1107 50-51 0.724651947988086 1107 52-53 0.7228126694708408 1107 54-55 0.8583248134360346 1107 56-57 0.9808511682434897 1107 58-59 0.8711677249144643 1107 60-61 0.926855456184029 1107 62-63 0.7757179104826903 1107 64-65 1.0019694604411384 1107 66-67 0.8112165951377008 1107 68-69 0.8831625566733337 1107 70-71 0.9099523267212746 1107 72-73 0.8012245555850619 1107 74-75 0.8807814879276208 1107 76-77 0.6887001481037629 1107 78-79 0.8950776677375352 1107 80-81 0.8576294162355751 1107 82-83 0.9593492695657453 1107 84-85 0.9334567716174611 1107 86-87 0.8228087548265393 1107 88-89 0.7030060099936684 1107 90-91 0.8110882486430917 1107 92-93 0.9404378648651992 1107 94-95 0.9922719298090037 1107 96-97 1.1482434857334134 1107 98-99 1.142468537021017 1107 100-101 1.1226766189067838 1108 1 0.002972619588305747 1108 2 0.1830731720887897 1108 3 0.1587055213540367 1108 4 0.1706951047188312 1108 5 0.08798363601544423 1108 6 0.12323737360000564 1108 7 0.09496480547283426 1108 8 -0.04044168016046967 1108 9 -0.02364306442596842 1108 10-11 -0.057429822380029805 1108 12-13 -0.1640007326216022 1108 14-15 0.24463693997156355 1108 16-17 0.20365853596358363 1108 18-19 0.2342475057942508 1108 20-21 0.30037118079116 1108 22-23 0.20364066415409354 1108 24-25 0.2684185232658365 1108 26-27 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-1.5094300379299241 2316 66-67 -1.5235333826406752 2316 68-69 -1.0182689021857527 2316 70-71 -1.5867190676757907 2316 72-73 -1.446766030176871 2316 74-75 -1.7649679990427813 2316 76-77 -2.112958550984569 2316 78-79 -2.3151288194299617 2316 80-81 -2.1370710169894167 2316 82-83 -1.9901226287790337 2316 84-85 -1.6814668826218444 2316 86-87 -1.5813638859136177 2316 88-89 -2.0815219448056226 2316 90-91 -2.026382428478687 2316 92-93 -2.3584460300632486 2316 94-95 -2.3613525132012327 2316 96-97 -2.3527052808700013 2316 98-99 -2.4174390210353813 2316 100-101 -2.0835755791013852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6309.0 3 2037.0 4 2122.0 5 2295.0 6 2568.0 7 2960.0 8 3823.0 9 4650.0 10 5692.0 11 7352.0 12 9429.0 13 11303.0 14 12803.0 15 15986.0 16 19801.0 17 26421.0 18 22238.0 19 14729.0 20 13898.0 21 14832.0 22 16265.0 23 17840.0 24 20286.0 25 23329.0 26 27559.0 27 32272.0 28 38409.0 29 46370.0 30 56377.0 31 69637.0 32 87254.0 33 112509.0 34 152556.0 35 213026.0 36 313884.0 37 442696.0 38 381617.0 39 64988.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.294613420996065 19.044544143151768 14.848939768417763 21.811902667434406 2 15.429492767985256 20.656311221249087 40.39953659144266 23.514659419322996 3 18.877887332881222 26.198327592734167 30.049845869337084 24.873939205047527 4 12.750368120127456 17.000519622778913 37.559051478938 32.690060778155626 5 13.585314844459493 38.144594785419 33.15923183453462 15.11085853558688 6 30.70046459849302 36.239330190524676 18.092784106145576 14.96742110483672 7 28.000906921756407 33.83928050873992 20.727739929389664 17.43207264011401 8 26.977625264965106 33.77079293276698 19.924209940881855 19.327371861386062 9 27.60230008370066 16.474777078976626 20.29844596643163 35.62447687089109 10-11 24.793705001174633 27.087392728328012 26.966150691651812 21.152751578845542 12-13 26.240321558291726 24.622771777897142 27.69675108334643 21.440155580464705 14-15 23.389352385899528 25.275761110596555 26.603922391579804 24.730964111924113 16-17 21.88050683443971 28.933163210993662 26.767925302790196 22.418404651776427 18-19 21.79970506193764 26.946311644311 29.9204971159237 21.33348617782766 20-21 23.187512370833392 25.846337682902167 29.699929180014216 21.26622076625023 22-23 22.806692629378617 26.09513732306049 29.615191510616263 21.48297853694463 24-25 22.340236109789174 26.189111859165493 29.869794077852475 21.60085795319286 26-27 22.08818735253425 26.390966361029182 29.62034899517729 21.90049729125928 28-29 21.863546467469135 26.34499451018244 29.8650176133895 21.926441408958926 30-31 22.03666018545893 26.198717713550764 29.906148371461683 21.858473729528626 32-33 21.915641658318847 26.311204317011892 29.704338375975897 22.06881564869336 34-35 22.282147209172297 26.325238820602024 29.53253115419582 21.86008281602986 36-37 22.514570349750976 26.02056109576885 29.412824670034748 22.052043884445425 38-39 22.24025133236539 26.12286420145261 29.573785506005358 22.063098960176646 40-41 22.231416802858696 26.187201661758575 29.552741927546705 22.028639607836027 42-43 22.256306150171774 26.231025002730213 29.172341126281854 22.340327720816155 44-45 22.188042591153156 26.491309693928418 29.006213619553158 22.31443409536527 46-47 22.5368012837484 26.547867723165535 28.574406204870247 22.340924788215823 48-49 22.597732838112762 26.780299531911254 28.11836180161082 22.50360582836516 50-51 22.55871087532813 26.94481224105758 27.94300877310889 22.5534681105054 52-53 22.707200262388753 27.043908038784338 27.84835829011435 22.40053340871256 54-55 22.422082635953466 26.98065952354029 27.88046297678988 22.716794863716366 56-57 22.52383834917881 27.324569496192662 27.648837944544614 22.502754210083918 58-59 22.76795127371909 26.92033355799875 27.674527366340808 22.637187801941362 60-61 22.655369731133078 26.868218844315727 28.134894215320138 22.341517209231057 62-63 22.599644103151814 26.818500161329833 28.177296629987058 22.404559105531295 64-65 22.519040483901037 26.912140164200437 28.014390842614777 22.554428509283746 66-67 22.454219055707657 27.199659846693596 27.728041388196722 22.61807970940202 68-69 22.36488777254061 27.131838319601858 27.954949750299917 22.548324157557612 70-71 22.465961546925474 27.054065412858368 27.809202572053128 22.67077046816303 72-73 22.476951009461217 27.166851047085178 27.5150884163074 22.841109527146205 74-75 22.451526405533045 27.446064516101192 27.41786507115775 22.68454400720801 76-77 22.459210846868938 27.31817535446336 27.448357979128918 22.774255819538787 78-79 22.477158372716566 27.356815862156637 27.383233221469627 22.78279254365717 80-81 22.418081178263847 27.543466777606497 27.358046791520007 22.68040525260965 82-83 22.614871723041855 27.454547377322 27.37588031037773 22.554700589258417 84-85 22.505134135010213 27.35892325075191 27.200674263279957 22.935268350957923 86-87 22.54908520476702 27.072016382742863 27.457260345405132 22.921638067084984 88-89 22.691947081419066 27.067049761695404 27.284834492887345 22.956168663998184 90-91 22.541028364921615 27.091778614529993 27.573146153311555 22.794046867236833 92-93 22.607965881415122 27.200712832869574 27.289039870850846 22.902281414864454 94-95 22.457114880139265 27.012207126537223 27.29312128376453 23.23755670955898 96-97 22.493240495026335 27.017488788850425 27.304384546100646 23.1848861700226 98-99 22.35994449933324 27.144101974376834 27.227590526014588 23.268363000275343 100-101 22.35350492052784 27.06320543905551 27.33983126904097 23.243458371375684 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 607.0 1 462.0 2 370.0 3 436.5 4 440.0 5 508.5 6 801.0 7 1521.0 8 2714.5 9 3454.0 10 3250.0 11 2973.5 12 3080.5 13 3330.5 14 3584.0 15 3804.0 16 3928.0 17 4103.5 18 4313.0 19 4544.0 20 4881.0 21 5395.0 22 6216.0 23 7249.5 24 8487.5 25 10345.0 26 12777.0 27 15548.0 28 17942.5 29 20550.5 30 24680.0 31 29462.5 32 34152.0 33 38651.5 34 43721.0 35 48024.0 36 53115.0 37 58721.5 38 61664.0 39 63487.5 40 65485.0 41 69863.5 42 73286.5 43 77019.0 44 82643.5 45 87124.5 46 87996.0 47 87225.5 48 90448.5 49 89751.5 50 84710.5 51 80778.0 52 77678.5 53 77425.0 54 77038.0 55 72964.0 56 67271.0 57 61008.0 58 55804.5 59 50521.0 60 43254.5 61 35414.5 62 28476.5 63 22496.0 64 17160.0 65 13013.5 66 9294.0 67 6882.5 68 4783.0 69 2807.5 70 1870.5 71 1199.5 72 751.5 73 550.0 74 391.5 75 266.5 76 172.0 77 79.5 78 44.0 79 35.0 80 32.5 81 24.0 82 18.5 83 17.5 84 13.0 85 13.0 86 11.0 87 7.5 88 7.0 89 5.0 90 2.5 91 2.0 92 3.0 93 3.0 94 2.5 95 1.0 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05970298627090144 2 0.09744873264881962 3 0.06906393137262515 4 0.12945712557729422 5 0.10538612388714755 6 0.10262529215207697 7 0.11289213516687072 8 0.09796638859914536 9 0.11854321262459332 10-11 0.10896657754356721 12-13 0.09360945101723708 14-15 0.09634871375437742 16-17 0.11015287242973036 18-19 0.08897211646223571 20-21 0.07357185194004509 22-23 0.07518952678481301 24-25 0.06321873293353039 26-27 0.055669583657946754 28-29 0.06817960245748535 30-31 0.051204801086387294 32-33 0.05042831716089869 34-35 0.04652432853552543 36-37 0.05148519805948039 38-39 0.05219697499117828 40-41 0.06729527354234555 42-43 0.06362854389420493 44-45 0.048961625301642435 46-47 0.037335935417243636 48-49 0.04572627561210659 50-51 0.028578922257566624 52-53 0.04182228698673334 54-55 0.04900476329750291 56-57 0.05325385588975999 58-59 0.05881865735576164 60-61 0.05562644566208628 62-63 0.06295990495836752 64-65 0.04201640796810549 66-67 0.06641094462720576 68-69 0.054461719773853376 70-71 0.0265730054500544 72-73 0.02027485805442462 74-75 0.03108092601747434 76-77 0.04367722080873389 78-79 0.06399521685901899 80-81 0.04184385598466358 82-83 0.06110497113636697 84-85 0.06641094462720576 86-87 0.0672089975506246 88-89 0.09030939433391051 90-91 0.06526778773690309 92-93 0.0755130617537666 94-95 0.08429164391137385 96-97 0.09248786312486465 98-99 0.12313740918373421 100-101 0.11819810865770951 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2318142.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.10649131397946 #Duplication Level Percentage of deduplicated Percentage of total 1 71.79954584908803 33.82224682887735 2 14.293198877225102 13.466048975179707 3 5.526172253554855 7.8095575578490815 4 2.622716287557573 4.94187848075453 5 1.4594056980660912 3.4373740919761224 6 0.8995774961143425 2.5425563704177 7 0.6110900512489769 2.0150415733853433 8 0.45143290343561526 1.7012336115627469 9 0.32688924325418717 1.3858744768188016 >10 1.7843242872704779 15.4135619320066 >50 0.14665182455452 4.799736184730892 >100 0.0757751547004419 6.369311510871463 >500 0.002392054919209622 0.7553548563411256 >1k 5.52012673663734E-4 0.36248712022703283 >5k 1.8400422455457798E-4 0.6438436941765663 >10k+ 9.200211227728899E-5 0.5338927348250619 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12319 0.5314169710052274 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9209 0.3972578038791411 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5647 0.2436002626241188 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.313799586047792E-5 0.0 5 0.0 0.0 0.0 4.313799586047792E-5 0.0 6 0.0 0.0 0.0 4.313799586047792E-5 0.0 7 0.0 0.0 0.0 4.313799586047792E-5 0.0 8 0.0 0.0 0.0 4.313799586047792E-5 0.0 9 0.0 0.0 0.0 4.313799586047792E-5 0.0 10-11 0.0 0.0 0.0 4.313799586047792E-5 0.0 12-13 0.0 0.0 0.0 4.313799586047792E-5 0.0 14-15 0.0 0.0 0.0 4.313799586047792E-5 0.0 16-17 0.0 0.0 0.0 8.627599172095584E-5 0.0 18-19 0.0 0.0 0.0 1.5098298551167273E-4 0.0 20-21 0.0 0.0 0.0 1.9412098137215064E-4 0.0 22-23 0.0 0.0 0.0 2.588279751628675E-4 2.156899793023896E-5 24-25 0.0 0.0 0.0 4.5294895653501813E-4 4.313799586047792E-5 26-27 0.0 0.0 0.0 6.470699379071687E-4 4.313799586047792E-5 28-29 0.0 2.156899793023896E-5 0.0 0.0013804158675352935 4.313799586047792E-5 30-31 0.0 4.313799586047792E-5 0.0 0.0030627977060939324 4.313799586047792E-5 32-33 0.0 4.313799586047792E-5 0.0 0.004702041548792093 4.313799586047792E-5 34-35 0.0 4.313799586047792E-5 0.0 0.006880510339746228 4.313799586047792E-5 36-37 0.0 4.313799586047792E-5 0.0 0.009188393118281797 4.313799586047792E-5 38-39 0.0 4.313799586047792E-5 0.0 0.012164914832654774 4.313799586047792E-5 40-41 0.0 4.313799586047792E-5 0.0 0.016672835400074713 4.313799586047792E-5 42-43 0.0 4.313799586047792E-5 0.0 0.021655273921959914 4.313799586047792E-5 44-45 0.0 4.313799586047792E-5 0.0 0.03373391276289373 4.313799586047792E-5 46-47 0.0 4.313799586047792E-5 0.0 0.05390092582766716 4.313799586047792E-5 48-49 0.0 4.313799586047792E-5 0.0 0.06884824139332275 4.313799586047792E-5 50-51 0.0 4.313799586047792E-5 0.0 0.08836818452018902 4.313799586047792E-5 52-53 0.0 4.313799586047792E-5 0.0 0.11051954539454442 4.313799586047792E-5 54-55 0.0 4.313799586047792E-5 0.0 0.13661803289013355 4.313799586047792E-5 56-57 0.0 4.313799586047792E-5 0.0 0.1651106791559792 4.313799586047792E-5 58-59 0.0 4.313799586047792E-5 0.0 0.20930555591503885 4.313799586047792E-5 60-61 0.0 4.313799586047792E-5 0.0 0.2643496386330087 4.313799586047792E-5 62-63 0.0 4.313799586047792E-5 0.0 0.32657619766174806 4.313799586047792E-5 64-65 0.0 4.313799586047792E-5 0.0 0.3755378229633905 4.313799586047792E-5 66-67 0.0 4.313799586047792E-5 0.0 0.44708218909799313 4.313799586047792E-5 68-69 0.0 4.313799586047792E-5 0.0 0.5366366685043453 4.313799586047792E-5 70-71 0.0 4.313799586047792E-5 0.0 0.6030044751356906 4.313799586047792E-5 72-73 0.0 4.313799586047792E-5 0.0 0.7129416575861185 4.313799586047792E-5 74-75 0.0 4.313799586047792E-5 0.0 0.800900031145633 4.313799586047792E-5 76-77 0.0 4.313799586047792E-5 0.0 0.8773836978062604 4.313799586047792E-5 78-79 0.0 4.313799586047792E-5 0.0 0.9707774588441951 4.313799586047792E-5 80-81 0.0 4.313799586047792E-5 0.0 1.0659398777124094 4.313799586047792E-5 82-83 0.0 4.313799586047792E-5 0.0 1.2036794984949153 4.313799586047792E-5 84-85 0.0 4.313799586047792E-5 0.0 1.3914160564797151 4.313799586047792E-5 86-87 0.0 4.313799586047792E-5 0.0 1.6418536914477198 8.627599172095584E-5 88-89 0.0 4.313799586047792E-5 0.0 1.9372626871002727 8.627599172095584E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7315 0.0 50.74256 1 GTATCAA 13355 0.0 43.345005 1 TATCAAC 17275 0.0 33.565014 2 ATCAACG 17315 0.0 33.538025 3 TCAACGC 17390 0.0 33.42143 4 CAACGCA 17765 0.0 32.76944 5 AACGCAG 18300 0.0 31.914612 6 ACGCAGA 20560 0.0 28.174746 7 CGCAGAG 20630 0.0 27.823961 8 GCAGAGT 21415 0.0 25.672403 9 GTACATG 15275 0.0 25.295603 1 TACATGG 15695 0.0 24.376966 2 GAGTACT 10400 0.0 23.777617 12-13 ACATGGG 15975 0.0 22.875694 3 GTACTTT 11245 0.0 21.844637 14-15 AGTACTT 10985 0.0 21.646364 12-13 CAGAGTA 20890 0.0 21.197363 10-11 AGAGTAC 19770 0.0 21.064426 10-11 TACACCG 260 1.48393156E-8 20.105623 5 CATGGGA 9565 0.0 20.072182 4 >>END_MODULE