FastQCFastQC Report
Thu 26 May 2016
SRR937131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318142
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121610.5246011676592719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95200.4106737205917498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61700.26616143445914875No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24620.10620574580849663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75750.048.2305261
GTATCAA131700.041.268491
TATCAAC174300.031.0964182
ATCAACG174400.031.0234413
TCAACGC177000.030.567074
CAACGCA180950.029.977925
AACGCAG186100.029.2249056
ACGCAGA213000.025.3105097
CGCAGAG214350.025.0403048
GTACATG150400.023.9757521
GCAGAGT225050.023.3221139
TACATGG154600.022.8912162
GAGTACT112600.022.0403512-13
ACATGGG157750.021.5001543
AGTACTT115800.021.1851912-13
GTGGTAT40150.021.062641
GTACTTT119250.020.71146814-15
AGAGTAC207050.020.40566310-11
TGGTATC40300.020.3921572
CAGAGTA220400.019.7623110-11
Produced by FastQC (version 0.11.5)