##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937131_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2318142 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03052099483121 31.0 31.0 34.0 30.0 34.0 2 32.015065513674315 33.0 31.0 34.0 30.0 34.0 3 32.19193302222211 33.0 31.0 34.0 30.0 34.0 4 35.951892938396355 37.0 35.0 37.0 35.0 37.0 5 35.86648747143186 37.0 35.0 37.0 35.0 37.0 6 35.910542149704376 37.0 35.0 37.0 35.0 37.0 7 35.84061890945421 37.0 35.0 37.0 35.0 37.0 8 35.822053610175736 37.0 35.0 37.0 35.0 37.0 9 37.47634959376949 39.0 37.0 39.0 35.0 39.0 10-11 37.39987153504833 39.0 37.0 39.0 34.0 39.0 12-13 37.29847373456846 39.0 37.0 39.0 34.0 39.0 14-15 38.46348088253437 40.0 38.0 41.0 34.0 41.0 16-17 38.37879495734084 40.0 38.0 41.0 33.5 41.0 18-19 38.45557023685348 40.0 38.0 41.0 33.5 41.0 20-21 38.54305560228838 40.0 38.0 41.0 34.0 41.0 22-23 38.471477804206984 40.0 38.0 41.0 34.0 41.0 24-25 38.36622109430742 40.0 38.0 41.0 34.0 41.0 26-27 38.199456720080136 40.0 38.0 41.0 33.5 41.0 28-29 38.01545612822683 40.0 37.0 41.0 33.0 41.0 30-31 37.861663996424724 40.0 37.0 41.0 33.0 41.0 32-33 37.830504127874825 40.0 37.0 41.0 33.0 41.0 34-35 37.73945642674176 40.0 37.0 41.0 33.0 41.0 36-37 37.56997241756545 40.0 37.0 41.0 33.0 41.0 38-39 37.40968780169636 40.0 36.0 41.0 32.5 41.0 40-41 37.20876654665676 40.0 36.0 41.0 31.5 41.0 42-43 36.96286616609336 39.5 36.0 41.0 31.0 41.0 44-45 36.608469412141275 39.0 35.5 41.0 30.5 41.0 46-47 36.26946213821241 39.0 35.0 41.0 30.0 41.0 48-49 35.910032258593304 39.0 35.0 41.0 29.0 41.0 50-51 35.63675262343722 39.0 35.0 40.0 28.0 41.0 52-53 35.36391170169903 38.5 35.0 40.0 27.5 41.0 54-55 35.11228345804528 38.0 34.5 40.0 27.0 41.0 56-57 34.83827824179882 38.0 34.0 40.0 26.0 41.0 58-59 34.50186593401095 38.0 34.0 40.0 26.0 41.0 60-61 34.17341754732885 37.0 33.5 40.0 25.5 41.0 62-63 33.80179169352007 37.0 33.0 39.5 24.5 41.0 64-65 33.38930617710218 36.0 33.0 39.0 23.0 40.5 66-67 33.01072690974064 36.0 32.0 39.0 22.5 40.0 68-69 32.57430713907949 35.0 32.0 38.5 22.0 40.0 70-71 32.106567457903786 35.0 31.0 38.0 20.5 40.0 72-73 31.782949016928214 35.0 31.0 37.0 20.0 39.0 74-75 31.342701180514396 35.0 31.0 37.0 18.5 39.0 76-77 29.931513686391945 33.5 29.5 35.0 17.5 37.5 78-79 30.67204554337051 34.0 30.5 36.0 18.0 38.0 80-81 30.517726265258986 34.0 30.5 35.5 16.0 37.0 82-83 30.20604389204803 34.0 30.0 35.0 12.0 37.0 84-85 29.839124393587625 34.0 30.0 35.0 7.5 36.0 86-87 29.479475157259564 34.0 29.5 35.0 6.0 36.0 88-89 29.13616810359331 34.0 29.0 35.0 2.0 35.5 90-91 28.731399327564922 34.0 29.0 35.0 2.0 35.0 92-93 28.278527588042493 33.5 29.0 35.0 2.0 35.0 94-95 27.81515994274725 33.0 27.5 35.0 2.0 35.0 96-97 27.210534557417105 33.0 26.5 35.0 2.0 35.0 98-99 26.487547139044977 33.0 25.0 34.5 2.0 35.0 100-101 23.562898864694226 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3739598704340139 1101 2 0.2658193692566755 1101 3 0.2169575427209125 1101 4 0.24883426306986678 1101 5 0.286452776556807 1101 6 0.24721173394392082 1101 7 0.23803591939870472 1101 8 0.2867823805033396 1101 9 0.3293758181021573 1101 10-11 0.3826601022626619 1101 12-13 0.4054745437721863 1101 14-15 0.5293147516280499 1101 16-17 0.4646986881999595 1101 18-19 0.47480494377273175 1101 20-21 0.48880932950134337 1101 22-23 0.482836242913244 1101 24-25 0.5236801779701494 1101 26-27 0.5053617361225733 1101 28-29 0.4711246014972872 1101 30-31 0.5522693607965365 1101 32-33 0.7078957263976093 1101 34-35 0.6268977986058744 1101 36-37 0.6330227120891081 1101 38-39 0.6214734142774248 1101 40-41 0.6230917502019864 1101 42-43 0.7188094409038186 1101 44-45 0.8438602153595198 1101 46-47 0.6589146644916326 1101 48-49 0.488384074853478 1101 50-51 0.3407271562587013 1101 52-53 0.36078923659031403 1101 54-55 0.39008740741746806 1101 56-57 0.6976034138860285 1101 58-59 0.6200448173260114 1101 60-61 0.5042096412042554 1101 62-63 0.6210736152510776 1101 64-65 0.6299255309394098 1101 66-67 0.6794000129936109 1101 68-69 0.5506733810499327 1101 70-71 0.5084976540123662 1101 72-73 0.5901810931986482 1101 74-75 0.5426021787964999 1101 76-77 0.4043161186077633 1101 78-79 0.7251560050324137 1101 80-81 0.8589813007831744 1101 82-83 0.94401769979779 1101 84-85 0.8437526044215709 1101 86-87 0.49348396023860275 1101 88-89 0.7082384612914652 1101 90-91 0.9062749795600951 1101 92-93 0.8374308324654614 1101 94-95 0.9200419244741767 1101 96-97 0.9416543487563942 1101 98-99 0.9140886005840692 1101 100-101 1.1693339797970204 1102 1 0.2005422309906848 1102 2 0.057872823163165776 1102 3 0.16526792637783672 1102 4 0.14024083220311212 1102 5 0.1695587744516729 1102 6 0.1942257544635524 1102 7 0.2156640373811527 1102 8 0.24346768500170413 1102 9 0.30869322822883305 1102 10-11 0.22878235981054473 1102 12-13 0.2701079072920365 1102 14-15 0.34245626010368824 1102 16-17 0.399283789317586 1102 18-19 0.29794668999419827 1102 20-21 0.3912149930391351 1102 22-23 0.39553997487428205 1102 24-25 0.3135853822262291 1102 26-27 0.2607583501897821 1102 28-29 0.28310320869579897 1102 30-31 0.2448214325352538 1102 32-33 0.28031072686229663 1102 34-35 0.2605154903532849 1102 36-37 0.3694115475452193 1102 38-39 0.3468835798565806 1102 40-41 0.3147534638306624 1102 42-43 0.2666964208618481 1102 44-45 0.3571081691244373 1102 46-47 0.22119229109669192 1102 48-49 0.13779197968599277 1102 50-51 0.19797613449198792 1102 52-53 0.20152817222386687 1102 54-55 0.034737910249887705 1102 56-57 -0.057288854835910286 1102 58-59 -0.10980309018653855 1102 60-61 -0.13861065105020742 1102 62-63 -0.08488634835220665 1102 64-65 -0.1616127709934645 1102 66-67 -0.03740872481380819 1102 68-69 -0.15862194032839483 1102 70-71 -0.1363204596258214 1102 72-73 0.029238085195256502 1102 74-75 -0.25424812130356855 1102 76-77 -0.26172415985744024 1102 78-79 -0.1551300031935945 1102 80-81 -0.18836318643150562 1102 82-83 -0.20525414958374455 1102 84-85 -0.032950466173804926 1102 86-87 -0.06966932746194132 1102 88-89 0.05113753386661912 1102 90-91 0.014494501085820843 1102 92-93 -0.08331432778603087 1102 94-95 0.1748767019332753 1102 96-97 0.4275391630661005 1102 98-99 0.4987479432384774 1102 100-101 0.5989360365145266 1103 1 0.05521162078927233 1103 2 -0.09175824279199318 1103 3 0.025773109732291744 1103 4 0.06404134954519947 1103 5 0.16169056555990835 1103 6 0.15648680162432527 1103 7 0.2204556105063844 1103 8 0.24996942735581484 1103 9 0.1817298514392931 1103 10-11 0.24984418258711116 1103 12-13 0.29571196973864033 1103 14-15 0.34092310769439393 1103 16-17 0.3910255871409305 1103 18-19 0.33701853379026403 1103 20-21 0.26152245794371254 1103 22-23 0.31393085026317635 1103 24-25 0.3744767840961316 1103 26-27 0.2898310284085639 1103 28-29 0.32175784113258743 1103 30-31 0.32645797389282194 1103 32-33 0.3925678005815598 1103 34-35 0.40474426522205675 1103 36-37 0.38078433185489047 1103 38-39 0.4249388583749649 1103 40-41 0.3800241942684224 1103 42-43 0.43439512273850767 1103 44-45 0.47537646384254373 1103 46-47 0.38061232757288366 1103 48-49 0.37535987586790753 1103 50-51 0.3564155256018111 1103 52-53 0.43768350994653105 1103 54-55 0.37889913661287267 1103 56-57 0.3399692718453551 1103 58-59 0.39502225719158446 1103 60-61 0.49889426608367415 1103 62-63 0.49159763271832446 1103 64-65 0.5466921191210119 1103 66-67 0.6276911476387212 1103 68-69 0.7253728385694629 1103 70-71 0.6202885656492398 1103 72-73 0.6556656433962438 1103 74-75 0.6576360449293297 1103 76-77 0.6440214365760006 1103 78-79 0.5942211338066947 1103 80-81 0.685068595537814 1103 82-83 0.5754333352675083 1103 84-85 0.6431784878971847 1103 86-87 0.3897016370115196 1103 88-89 0.6677189039887246 1103 90-91 0.8848132902987231 1103 92-93 0.8817487903324626 1103 94-95 0.924610481351646 1103 96-97 1.040817808599357 1103 98-99 0.9780836632856804 1103 100-101 0.8576519791539248 1104 1 0.2384872147465913 1104 2 0.09655055907555266 1104 3 0.12882652207348144 1104 4 0.12815741501381694 1104 5 0.08660004090848616 1104 6 0.1787206255869762 1104 7 0.2620201300638101 1104 8 0.2850709902345514 1104 9 0.22719727934587297 1104 10-11 0.2523656344123282 1104 12-13 0.29144416593567257 1104 14-15 0.32005078940561305 1104 16-17 0.3568673079110738 1104 18-19 0.38628781059991724 1104 20-21 0.3267893331930978 1104 22-23 0.28310622755799386 1104 24-25 0.32960452547344943 1104 26-27 0.1313028790643358 1104 28-29 0.3658119284464618 1104 30-31 0.3602860385857838 1104 32-33 0.4233689297623755 1104 34-35 0.30292109314877536 1104 36-37 0.2858908171863561 1104 38-39 0.4166191674453259 1104 40-41 0.5572054561317188 1104 42-43 0.3760804661355195 1104 44-45 0.5121324556235436 1104 46-47 0.6531918419654659 1104 48-49 0.5827683536739912 1104 50-51 0.5796314161742018 1104 52-53 0.3933309057080052 1104 54-55 0.4595263018394746 1104 56-57 0.42166744345249896 1104 58-59 0.47529329042186674 1104 60-61 0.46202621392058774 1104 62-63 0.4900744692220371 1104 64-65 0.5007652622935339 1104 66-67 0.6793297277348529 1104 68-69 0.4973489795902708 1104 70-71 0.4498628107799263 1104 72-73 0.5130996657486513 1104 74-75 0.5648442684086845 1104 76-77 0.5037339578786622 1104 78-79 0.49161391197188564 1104 80-81 0.5294312684296436 1104 82-83 0.6061087927386382 1104 84-85 0.6779723974806764 1104 86-87 0.5668832717879049 1104 88-89 0.6650121143601844 1104 90-91 0.7547495967437783 1104 92-93 0.9450858403662146 1104 94-95 0.8906353024465936 1104 96-97 0.8033456372383334 1104 98-99 0.9226134012396585 1104 100-101 0.9227217698378105 1105 1 0.10959909900013542 1105 2 0.06742733774244414 1105 3 0.03673506635169588 1105 4 0.18327509080707927 1105 5 0.1139485489192964 1105 6 0.06406852211240732 1105 7 0.13039684723073464 1105 8 0.08692020637354858 1105 9 0.18999085382935732 1105 10-11 0.23023935213802815 1105 12-13 0.23468531960475758 1105 14-15 0.2337403305455794 1105 16-17 0.34883014084552855 1105 18-19 0.21416644832294907 1105 20-21 0.15844951600614365 1105 22-23 0.2008034579509257 1105 24-25 0.0941292361600361 1105 26-27 0.21045298466855655 1105 28-29 0.23513592081343404 1105 30-31 0.2363309230185635 1105 32-33 0.20156648874084482 1105 34-35 0.23116258067482676 1105 36-37 0.22608596536613135 1105 38-39 0.369558514421108 1105 40-41 0.07807889622899467 1105 42-43 0.0949347744717528 1105 44-45 0.27508803859051056 1105 46-47 0.15852757906892379 1105 48-49 0.23193704983955143 1105 50-51 0.2609108263368043 1105 52-53 0.35488569514969015 1105 54-55 0.266442939513297 1105 56-57 0.323786908016217 1105 58-59 0.2753266761180342 1105 60-61 0.45754971920782594 1105 62-63 0.2786867839753526 1105 64-65 0.23185753684344235 1105 66-67 0.22515282571659867 1105 68-69 0.18676518676432607 1105 70-71 0.06918697886109726 1105 72-73 0.19670289607832459 1105 74-75 0.2703887539702521 1105 76-77 0.17245243914577202 1105 78-79 0.26369837426889475 1105 80-81 0.47686689553601624 1105 82-83 0.4817634159546955 1105 84-85 0.5734271985195001 1105 86-87 0.47059997834168854 1105 88-89 0.600248959517323 1105 90-91 0.7170126900866691 1105 92-93 0.7856816168999359 1105 94-95 0.8312757103846558 1105 96-97 0.7600614335926146 1105 98-99 0.7130505078595135 1105 100-101 0.6910571941700105 1106 1 0.2448997112655178 1106 2 0.09574210237157388 1106 3 0.14457407066766592 1106 4 0.1526718588488336 1106 5 0.12181766464748733 1106 6 0.1834703087228604 1106 7 0.19983376382212725 1106 8 0.18381873091435352 1106 9 0.3589861363747815 1106 10-11 0.2995558230805102 1106 12-13 0.3318598654242919 1106 14-15 0.4611467342392501 1106 16-17 0.43989206998335106 1106 18-19 0.4143973024833443 1106 20-21 0.37516134979114923 1106 22-23 0.45516961272358003 1106 24-25 0.4291889571276144 1106 26-27 0.43037670242426174 1106 28-29 0.5279694047038319 1106 30-31 0.5015923904480459 1106 32-33 0.504943985478775 1106 34-35 0.4315640661711626 1106 36-37 0.36935606473379323 1106 38-39 0.5613682102958464 1106 40-41 0.3836996931776966 1106 42-43 0.3700627479737051 1106 44-45 0.5083460933932784 1106 46-47 0.43318882568138406 1106 48-49 0.43095842894224035 1106 50-51 0.3214749134591628 1106 52-53 0.4512371957017294 1106 54-55 0.4939798259932786 1106 56-57 0.5254530728260107 1106 58-59 0.5313088100134493 1106 60-61 0.5805096710295672 1106 62-63 0.599122395738874 1106 64-65 0.6069955474828035 1106 66-67 0.6990964170043625 1106 68-69 0.7430785461942193 1106 70-71 0.685888775508694 1106 72-73 0.7016110562710445 1106 74-75 0.7056191062737724 1106 76-77 0.6622798003823469 1106 78-79 0.6758282548269605 1106 80-81 0.5929815196452211 1106 82-83 0.8237136317531508 1106 84-85 0.7024947484542565 1106 86-87 0.7784088712454356 1106 88-89 0.9893937954101268 1106 90-91 1.1156024722807878 1106 92-93 1.0363290122627795 1106 94-95 1.1410418450830413 1106 96-97 1.2177699551222751 1106 98-99 1.2055979333337703 1106 100-101 1.1297152288985082 1107 1 0.17124787155635346 1107 2 0.06659339676367892 1107 3 0.10684729217788913 1107 4 0.15786377770624682 1107 5 0.1433979802469807 1107 6 0.18358860793708232 1107 7 0.17296052428185504 1107 8 0.15219641265450434 1107 9 0.2654285680767572 1107 10-11 0.17050738663552067 1107 12-13 0.17414916679660308 1107 14-15 0.2156396206480835 1107 16-17 0.3048726508821602 1107 18-19 0.2213215781076272 1107 20-21 0.18138834077796417 1107 22-23 0.24439803691549855 1107 24-25 0.24431865390999263 1107 26-27 0.29582716853787616 1107 28-29 0.32397326460665 1107 30-31 0.19216515734340334 1107 32-33 0.30201069850842543 1107 34-35 0.31925524450860365 1107 36-37 0.28961089559167164 1107 38-39 0.298877616279249 1107 40-41 0.30857389677377256 1107 42-43 0.3454829125623178 1107 44-45 0.4200429052018606 1107 46-47 0.35776571741093477 1107 48-49 0.30626507779962964 1107 50-51 0.32608352336060165 1107 52-53 0.36879993752059903 1107 54-55 0.3190742537875195 1107 56-57 0.32510654627860447 1107 58-59 0.4940128209639383 1107 60-61 0.5532815338466364 1107 62-63 0.4237212730922124 1107 64-65 0.308845375553382 1107 66-67 0.24487112967342028 1107 68-69 0.3794323730726106 1107 70-71 0.3897337759410462 1107 72-73 0.4440388946702676 1107 74-75 0.43078326253176513 1107 76-77 0.4768681356750655 1107 78-79 0.4081486856347958 1107 80-81 0.6324253845523025 1107 82-83 0.4601890694878392 1107 84-85 0.6078299878215851 1107 86-87 0.6286707669397451 1107 88-89 0.5304128980655101 1107 90-91 0.6623237626437266 1107 92-93 0.7619331569668191 1107 94-95 0.9192640268296373 1107 96-97 1.0324332623120362 1107 98-99 0.8975190904951056 1107 100-101 0.7034879435435073 1108 1 -0.018352634717459182 1108 2 -0.14025430990590237 1108 3 -0.023954136696424655 1108 4 0.04394092663940796 1108 5 0.03400573368906379 1108 6 0.054521391736322755 1108 7 0.12254740999118496 1108 8 0.13209098177566148 1108 9 0.12680720049498717 1108 10-11 0.08101144389328852 1108 12-13 0.1462653977551156 1108 14-15 0.022954088642968884 1108 16-17 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70-71 -0.8193794074704179 2316 72-73 -0.7141809795746816 2316 74-75 -0.7912424217975484 2316 76-77 -1.0832620250548821 2316 78-79 -0.8727990761648847 2316 80-81 -0.9473274337289403 2316 82-83 -0.8097406328970216 2316 84-85 -0.8227160457156693 2316 86-87 -0.7419908963196029 2316 88-89 -0.9510488594908075 2316 90-91 -0.9557549753227832 2316 92-93 -1.1151078506194487 2316 94-95 -1.0507004572229661 2316 96-97 -0.8971995592154975 2316 98-99 -0.9863661763769045 2316 100-101 -0.9398940712425663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 27.0 5 67.0 6 140.0 7 336.0 8 795.0 9 1489.0 10 2584.0 11 3966.0 12 6139.0 13 8444.0 14 10407.0 15 14316.0 16 19150.0 17 26023.0 18 22771.0 19 13756.0 20 12149.0 21 12517.0 22 13113.0 23 14457.0 24 16694.0 25 20293.0 26 24569.0 27 30383.0 28 38810.0 29 48167.0 30 59841.0 31 73977.0 32 93006.0 33 120509.0 34 161114.0 35 225694.0 36 337782.0 37 495400.0 38 361823.0 39 27423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15348848045761 19.726442418251782 15.319207319835511 21.80086178145509 2 15.650692170641955 21.263464865168004 39.54177397576432 23.544068988425717 3 19.046328989968057 26.154104987069122 30.214206061442695 24.58535996152013 4 13.182013479795836 17.452729261786192 37.00693143111534 32.35832582730262 5 13.596856393722106 38.372772207607774 33.13046621762114 14.899905181048979 6 30.969332203688143 36.44986566770141 17.672160170344053 14.908641958266397 7 28.005242131518315 33.89703214774252 20.559232591977796 17.538493128761363 8 27.210800718851562 33.91168444383476 19.684126339111234 19.19338849820244 9 27.493872247688017 16.356763304404993 20.232323990506192 35.917040457400795 10-11 24.904033991066537 27.187978630267345 26.797665454415252 21.110321924250858 12-13 26.19727867092987 24.673314548093035 27.720121201036008 21.40928557994109 14-15 23.319537802257155 25.31247007301537 26.578268285549377 24.7897238391781 16-17 21.8345122947602 28.944344220500728 26.88987559864754 22.33126788609153 18-19 21.80638632145917 26.89927968174512 29.997277992461203 21.297056004334504 20-21 23.06341233200667 25.835275298973553 29.914869863137795 21.186442505881978 22-23 22.783785462667947 26.022370501893327 29.796988277681002 21.39685575775772 24-25 22.328333806072123 26.168195068921 30.029086916711336 21.47438420829554 26-27 22.077539431033546 26.398668218513627 29.817958515990266 21.705833834462567 28-29 21.8715805299026 26.34310691988046 30.055854715188406 21.729457835028533 30-31 22.06575524737712 26.083635310903507 30.136139067736025 21.714470373983346 32-33 21.936538256631124 26.239743309344078 29.903335503194185 21.920382930830616 34-35 22.28300527095586 26.24964922486514 29.762663548569602 21.70468195560939 36-37 22.56933285074526 25.972527854146815 29.56868618847595 21.889453106631976 38-39 22.297351221071512 26.09377905143336 29.75748968869589 21.851380038799235 40-41 22.283274666761 26.13203764295191 29.733436840162835 21.851250850124256 42-43 22.30163175884886 26.240966937530025 29.32142377220128 22.13597753141984 44-45 22.348597922810264 26.412991149770594 29.101492063437284 22.13691886398186 46-47 22.628000305417405 26.573233960517467 28.621299240468325 22.177466493596803 48-49 22.721241238114917 26.805663340863806 28.123143714111805 22.349951706909472 50-51 22.656366439163527 26.88066470632769 28.00400235275188 22.45896650175691 52-53 22.8085936109796 27.010969029827002 27.95904435387766 22.221393005315733 54-55 22.536283369850736 26.95238674536997 27.944805879711293 22.566524005068004 56-57 22.602602481694863 27.29834153209899 27.73511534171781 22.363940644488334 58-59 22.8320030162386 26.880630373160308 27.72573366595934 22.561632944641755 60-61 22.752179241567426 26.899284464197976 28.17924243019029 22.169293864044313 62-63 22.656398960891956 26.832113822190358 28.227369160301656 22.28411805661603 64-65 22.584682746385838 26.88490221136581 28.01037470814393 22.520040334104426 66-67 22.559055967284117 27.18968655905165 27.791073878195448 22.46018359546878 68-69 22.482838083870206 27.05440975022448 28.003445863852573 22.45930630205274 70-71 22.54093587180746 27.048562251255102 27.843014522862745 22.567487354074693 72-73 22.556302106666053 27.17612363179728 27.519178617871255 22.74839564366541 74-75 22.543714038964097 27.408143791515073 27.457536967233125 22.5906052022877 76-77 22.561994754800175 27.335066203446374 27.496748981232415 22.60619006052104 78-79 22.535748064444117 27.31362422122118 27.38594536766125 22.764682346673453 80-81 22.49382767178874 27.524312911910965 27.386327996421063 22.595531419879233 82-83 22.66142988069696 27.433400407275215 27.379153125516993 22.526016586510824 84-85 22.557441574840244 27.368164030903547 27.272501769805757 22.801892624450453 86-87 22.57619628556589 27.074141147115817 27.41521906809566 22.93444349922264 88-89 22.650717480432352 27.106196767024198 27.334680118993386 22.90840563355006 90-91 22.452177094010015 27.151534681312917 27.585460904795923 22.810827319881145 92-93 22.56771697666363 27.159737047919002 27.280027401266793 22.99251857415058 94-95 22.402776888053737 26.98680771111426 27.305749515396972 23.304665885435032 96-97 22.436032659547898 26.922816430891373 27.28460025615839 23.356550653402337 98-99 22.234305977360574 27.122381453616782 27.23665706101601 23.406655508006626 100-101 22.10767278255273 27.139388192825553 27.299971183495565 23.452967841126156 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 636.0 1 459.5 2 368.0 3 458.0 4 426.0 5 443.5 6 717.5 7 1544.5 8 2662.5 9 3342.5 10 3216.5 11 2911.5 12 2965.5 13 3292.0 14 3641.0 15 3834.0 16 4055.5 17 4354.5 18 4673.0 19 4860.5 20 5122.0 21 5719.0 22 6486.0 23 7450.0 24 8786.5 25 10709.0 26 13131.0 27 15600.0 28 17884.5 29 20670.5 30 24763.0 31 29488.0 32 33908.0 33 38457.5 34 43912.0 35 47873.5 36 52791.0 37 58330.5 38 61355.5 39 62791.5 40 64642.5 41 69297.0 42 72846.5 43 76508.0 44 83050.0 45 88061.0 46 88452.5 47 88087.5 48 90864.5 49 89655.5 50 84733.0 51 80879.5 52 77493.5 53 77097.0 54 76638.0 55 72754.5 56 66972.0 57 60577.5 58 55671.5 59 50730.5 60 43553.0 61 35502.0 62 28802.0 63 22793.5 64 17147.0 65 13000.0 66 9211.0 67 6796.5 68 4727.0 69 2720.0 70 1788.0 71 1156.5 72 735.0 73 511.5 74 349.0 75 249.5 76 152.0 77 66.0 78 35.0 79 20.5 80 13.0 81 10.5 82 7.0 83 2.0 84 1.5 85 2.5 86 2.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016608128406284 2 0.0031059357019544097 3 0.002027485805442462 4 2.588279751628675E-4 5 0.0017255198344191165 6 4.313799586047791E-4 7 4.313799586047792E-5 8 0.0 9 0.0 10-11 1.5098298551167273E-4 12-13 4.313799586047791E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0018980718178610281 22-23 0.0 24-25 0.003860850629512774 26-27 0.0018980718178610281 28-29 0.0032137806916056047 30-31 0.01574536848907444 32-33 9.058979130700362E-4 34-35 0.002652986745419392 36-37 0.0036667296481406233 38-39 0.0023725897723262854 40-41 0.004249092592257074 42-43 0.002609848749558914 44-45 4.313799586047791E-4 46-47 1.2941398758143375E-4 48-49 2.1568997930238956E-4 50-51 2.3725897723262852E-4 52-53 0.0010568808985817089 54-55 0.0029765217143729763 56-57 3.666729648140623E-4 58-59 9.92173904790992E-4 60-61 8.196219213490803E-4 62-63 0.0 64-65 1.941209813721506E-4 66-67 8.627599172095584E-5 68-69 2.156899793023896E-5 70-71 1.7255198344191168E-4 72-73 2.156899793023896E-5 74-75 3.4510396688382336E-4 76-77 9.706049068607532E-4 78-79 4.0981096067454017E-4 80-81 0.005823629441164518 82-83 0.0023078827785355683 84-85 0.00392555762330349 86-87 0.002156899793023896 88-89 0.0012941398758143376 90-91 2.588279751628675E-4 92-93 4.313799586047792E-5 94-95 3.235349689535844E-4 96-97 0.0019412098137215064 98-99 0.002674555743349631 100-101 0.001121587892372426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2318142.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.100277252421186 #Duplication Level Percentage of deduplicated Percentage of total 1 70.00077318422188 31.570542784922566 2 15.073951586087883 13.596787916442752 3 5.766543360272298 7.8021811300916735 4 2.9043651234003773 5.239506892304779 5 1.561984019910753 3.522295618091316 6 1.0227348084816217 2.767537405093381 7 0.6525195977146903 2.0600170338699604 8 0.4699138653368064 1.69545964891575 9 0.37041217628591644 1.5035122663350786 >10 1.9300580356795942 16.015228574065727 >50 0.15893637753217948 4.983896084896087 >100 0.08387164989655016 6.693178138779497 >500 0.0030721679448635335 0.9458586411572826 >1k 5.76031489661899E-4 0.39808763317921264 >5k 1.920104965539663E-4 0.679355554120279 >10k+ 9.600524827698315E-5 0.5265546777346536 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12161 0.5246011676592719 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9520 0.4106737205917498 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6170 0.26616143445914875 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2462 0.10620574580849663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.156899793023896E-5 0.0 18-19 0.0 0.0 0.0 8.627599172095584E-5 0.0 20-21 0.0 0.0 0.0 1.5098298551167273E-4 0.0 22-23 0.0 0.0 0.0 2.156899793023896E-4 0.0 24-25 0.0 0.0 0.0 4.745179544652571E-4 0.0 26-27 0.0 0.0 0.0 6.902079337676467E-4 0.0 28-29 0.0 0.0 0.0 0.0014235538633957713 0.0 30-31 0.0 0.0 0.0 0.003041228708163693 0.0 32-33 0.0 0.0 0.0 0.004702041548792093 0.0 34-35 0.0 0.0 0.0 0.0067726653500950335 0.0 36-37 0.0 0.0 0.0 0.009058979130700362 0.0 38-39 0.0 0.0 0.0 0.012035500845073339 0.0 40-41 0.0 0.0 0.0 0.016694404398004955 0.0 42-43 0.0 0.0 0.0 0.021870963901262305 0.0 44-45 0.0 0.0 0.0 0.03386332675047517 0.0 46-47 0.0 0.0 0.0 0.05441858177799289 0.0 48-49 0.0 0.0 0.0 0.06971100131053232 0.0 50-51 0.0 0.0 0.0 0.08918780644153809 0.0 52-53 0.0 0.0 0.0 0.1112313223262423 0.0 54-55 0.0 0.0 0.0 0.13737294781769194 0.0 56-57 0.0 0.0 0.0 0.16599500807111903 0.0 58-59 0.0 0.0 0.0 0.21016831583224843 0.0 60-61 0.0 0.0 0.0 0.265190829552288 0.0 62-63 0.0 0.0 0.0 0.3274820955748181 0.0 64-65 0.0 0.0 0.0 0.3763790138826698 0.0 66-67 0.0 0.0 0.0 0.44824691498622604 0.0 68-69 0.0 0.0 0.0 0.5388151372952994 0.0 70-71 0.0 0.0 0.0 0.6049456849494121 0.0 72-73 0.0 0.0 0.0 0.7152279713667238 0.0 74-75 0.0 0.0 0.0 0.8032726209179593 0.0 76-77 0.0 0.0 0.0 0.8803170815247728 0.0 78-79 0.0 0.0 0.0 0.9744441884923356 0.0 80-81 0.0 0.0 0.0 1.0702968152943177 0.0 82-83 0.0 0.0 0.0 1.2091148859733356 0.0 84-85 0.0 0.0 0.0 1.3970024269436472 0.0 86-87 0.0 0.0 0.0 1.6475910448971633 0.0 88-89 0.0 0.0 0.0 1.9433667135145303 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7575 0.0 48.230526 1 GTATCAA 13170 0.0 41.26849 1 TATCAAC 17430 0.0 31.096418 2 ATCAACG 17440 0.0 31.023441 3 TCAACGC 17700 0.0 30.56707 4 CAACGCA 18095 0.0 29.97792 5 AACGCAG 18610 0.0 29.224905 6 ACGCAGA 21300 0.0 25.310509 7 CGCAGAG 21435 0.0 25.040304 8 GTACATG 15040 0.0 23.975752 1 GCAGAGT 22505 0.0 23.322113 9 TACATGG 15460 0.0 22.891216 2 GAGTACT 11260 0.0 22.04035 12-13 ACATGGG 15775 0.0 21.500154 3 AGTACTT 11580 0.0 21.18519 12-13 GTGGTAT 4015 0.0 21.06264 1 GTACTTT 11925 0.0 20.711468 14-15 AGAGTAC 20705 0.0 20.405663 10-11 TGGTATC 4030 0.0 20.392157 2 CAGAGTA 22040 0.0 19.76231 10-11 >>END_MODULE