FastQCFastQC Report
Thu 26 May 2016
SRR937130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047229
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112400.5490348173067107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82910.404986447534692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51080.24950799348778274No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.10072151185822396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62150.047.9479981
GTATCAA112150.043.6921581
ATCAACG146750.033.2618983
TATCAAC147000.033.2061462
TCAACGC149900.032.5629354
CAACGCA151550.032.180195
AACGCAG156350.031.2499926
ACGCAGA176100.027.7438817
CGCAGAG177800.027.2661088
GTACATG133250.025.5025081
GCAGAGT185700.025.3895199
TACATGG136600.024.8782982
ACATGGG136800.023.7990263
GAGTACT90250.023.51240512-13
GTATACG1050.00218945222.6320971
AGTACTT94450.022.54233412-13
GTACTTT96500.021.81694414-15
CATGGGG55050.021.7569474
AGAGTAC172600.021.18907210-11
CAGAGTA182300.020.60911210-11
Produced by FastQC (version 0.11.5)