##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937130_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2047229 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.685905191847127 33.0 31.0 34.0 30.0 34.0 2 31.78735549369416 33.0 31.0 34.0 30.0 34.0 3 31.788740292365926 33.0 31.0 34.0 30.0 34.0 4 35.39613350533819 37.0 35.0 37.0 33.0 37.0 5 35.40305310251076 37.0 35.0 37.0 33.0 37.0 6 35.47705801353928 37.0 35.0 37.0 33.0 37.0 7 35.411031203641606 37.0 35.0 37.0 33.0 37.0 8 35.355925985808135 37.0 35.0 37.0 33.0 37.0 9 36.966476637445055 39.0 37.0 39.0 33.0 39.0 10-11 36.778448331867125 39.0 37.0 39.0 32.5 39.0 12-13 36.71053335997097 39.0 37.0 39.0 32.0 39.0 14-15 37.82883693030921 40.0 37.5 41.0 32.5 41.0 16-17 37.77002230820294 40.0 37.0 41.0 32.0 41.0 18-19 37.75370390903997 40.0 37.5 41.0 32.0 41.0 20-21 37.69424158215813 40.0 37.0 41.0 32.0 41.0 22-23 37.58046046631813 40.0 37.0 41.0 32.0 41.0 24-25 37.421970380450844 40.0 37.0 41.0 32.0 41.0 26-27 37.505924105217346 40.0 37.0 41.0 32.0 41.0 28-29 37.452274269268365 40.0 37.0 41.0 32.0 41.0 30-31 37.28271702872517 40.0 37.0 41.0 31.0 41.0 32-33 37.07142288429873 39.5 36.0 41.0 31.0 41.0 34-35 36.89482563992597 39.0 36.0 41.0 30.5 41.0 36-37 36.71769377045753 39.0 35.5 41.0 30.0 41.0 38-39 36.5453075840563 39.0 35.0 41.0 30.0 41.0 40-41 36.354541431368936 39.0 35.0 41.0 30.0 41.0 42-43 36.11371175379012 39.0 35.0 41.0 29.5 41.0 44-45 35.822248756734105 39.0 35.0 40.0 28.5 41.0 46-47 35.40013598869496 38.5 35.0 40.0 26.5 41.0 48-49 35.02786742469944 38.0 34.5 40.0 25.5 41.0 50-51 34.35599339399745 38.0 33.5 39.5 24.0 40.5 52-53 34.572270615549115 38.0 34.0 40.0 24.5 40.5 54-55 34.94379475867136 39.0 35.0 40.0 26.0 41.0 56-57 34.83225081317234 38.5 34.5 40.0 25.0 41.0 58-59 34.58069517381787 38.0 34.0 40.0 24.0 41.0 60-61 34.304072236178754 38.0 34.0 40.0 23.5 41.0 62-63 33.98153162152353 37.5 34.0 40.0 23.0 41.0 64-65 33.65600795025862 37.0 33.0 40.0 22.0 41.0 66-67 33.27896244142693 36.0 33.0 39.0 21.5 41.0 68-69 32.89942820270717 36.0 33.0 39.0 20.5 41.0 70-71 32.49336468953888 35.0 32.0 39.0 19.5 40.0 72-73 32.115059429111255 35.0 32.0 38.0 18.5 40.0 74-75 31.690018312558095 35.0 31.5 37.0 17.5 39.0 76-77 31.270091181787677 35.0 31.0 37.0 14.0 39.0 78-79 30.8484407460035 35.0 31.0 36.0 9.5 39.0 80-81 30.434185672438208 35.0 31.0 36.0 7.0 37.5 82-83 30.045381342292437 34.0 30.0 35.0 6.0 37.0 84-85 29.71078662914603 34.0 30.0 35.0 2.0 36.5 86-87 29.387564605620575 34.0 30.0 35.0 2.0 36.0 88-89 29.05523612649098 34.0 29.0 35.0 2.0 36.0 90-91 28.726554039631132 34.0 29.0 35.0 2.0 35.5 92-93 28.374557755873916 34.0 29.0 35.0 2.0 35.0 94-95 27.968223633018095 34.0 29.0 35.0 2.0 35.0 96-97 27.509038070484543 34.0 27.0 35.0 2.0 35.0 98-99 26.924058568924142 34.0 26.5 35.0 2.0 35.0 100-101 24.751536589214005 31.0 21.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1988790995141443 1101 2 0.27620819070031644 1101 3 0.2455026636856381 1101 4 0.12984471247727214 1101 5 0.06825682716078063 1101 6 0.07863719977867589 1101 7 0.041077666033686455 1101 8 0.18878136886295493 1101 9 0.08187866857770842 1101 10-11 0.23126592367565735 1101 12-13 0.18990798854502344 1101 14-15 0.4795080181489553 1101 16-17 0.5793790339984213 1101 18-19 0.3740985313894427 1101 20-21 0.4101198474650758 1101 22-23 0.4935922159675741 1101 24-25 0.3776945517759529 1101 26-27 0.5229413830629 1101 28-29 0.6538803519000425 1101 30-31 0.44177594586401625 1101 32-33 0.6504285334352318 1101 34-35 0.47536159860096205 1101 36-37 0.4019962486853004 1101 38-39 0.5807805037943652 1101 40-41 0.6529204223892933 1101 42-43 0.5928176626660644 1101 44-45 0.7500568576608018 1101 46-47 0.6976796473075169 1101 48-49 0.7425200062576636 1101 50-51 0.6208651987411784 1101 52-53 0.6884756213434358 1101 54-55 0.8217384579479585 1101 56-57 0.8468736765886788 1101 58-59 0.8302401243646713 1101 60-61 0.7930481751992247 1101 62-63 0.7170354145861211 1101 64-65 0.8227130910321279 1101 66-67 0.9431561395775603 1101 68-69 0.8144229726991696 1101 70-71 0.7884693702591221 1101 72-73 0.9247505485291612 1101 74-75 0.6757646567345468 1101 76-77 0.6566633205580743 1101 78-79 0.7269442731510303 1101 80-81 0.6391951647240113 1101 82-83 0.7982668934195907 1101 84-85 0.8722041456510432 1101 86-87 0.8073521420586793 1101 88-89 0.6938323918015215 1101 90-91 0.8813803545190702 1101 92-93 0.974787410182568 1101 94-95 0.9600946236977137 1101 96-97 0.846607792818979 1101 98-99 0.8729649151115844 1101 100-101 0.9338286332889503 1102 1 0.4369335582577172 1102 2 0.20600112730048892 1102 3 0.21463623145863053 1102 4 0.22516748728745029 1102 5 0.2404141422482695 1102 6 0.2507982599086631 1102 7 0.3200082233750692 1102 8 0.15142311352013138 1102 9 0.3051572701954868 1102 10-11 0.43002614816182927 1102 12-13 0.4439694871362647 1102 14-15 0.4628671298581679 1102 16-17 0.5124859170952547 1102 18-19 0.44796460534071514 1102 20-21 0.5205123612083398 1102 22-23 0.5190111717914618 1102 24-25 0.5559730348007221 1102 26-27 0.4599917959122308 1102 28-29 0.39695098298547293 1102 30-31 0.3728264885414845 1102 32-33 0.4032243116904297 1102 34-35 0.2604784460075322 1102 36-37 0.20802052405078086 1102 38-39 0.30718174265442855 1102 40-41 0.2788195966906457 1102 42-43 0.298230851124508 1102 44-45 0.23134007150647307 1102 46-47 0.385855445428092 1102 48-49 0.40033047964352164 1102 50-51 0.3735159606284597 1102 52-53 0.268284453568441 1102 54-55 0.3231060226341995 1102 56-57 0.38890584900045155 1102 58-59 0.41706799846632947 1102 60-61 0.23026732301897113 1102 62-63 0.14184734063235283 1102 64-65 0.2722828688720469 1102 66-67 0.10108603820757622 1102 68-69 -0.03651684571619995 1102 70-71 0.16191606186777108 1102 72-73 0.27300090628475004 1102 74-75 0.1909676567632559 1102 76-77 0.3623560348275596 1102 78-79 0.3529975143396733 1102 80-81 0.2060915065248814 1102 82-83 0.06817876424725 1102 84-85 0.24878689423760036 1102 86-87 0.2260016006711716 1102 88-89 0.1690778689344974 1102 90-91 0.2713757647614301 1102 92-93 0.4155175353460514 1102 94-95 0.317926984776399 1102 96-97 0.1794946338631469 1102 98-99 0.39498097967944545 1102 100-101 0.5524042729526109 1103 1 0.19962684954832355 1103 2 0.3151962383269833 1103 3 0.2803015068982937 1103 4 0.13200276317913762 1103 5 0.14573034618470615 1103 6 0.11403384208032463 1103 7 0.15737164045140872 1103 8 0.20932043850153548 1103 9 0.17541838893924222 1103 10-11 0.27604512491824806 1103 12-13 0.11257209161183823 1103 14-15 0.2790813395503662 1103 16-17 0.365587275767453 1103 18-19 0.29596015633647 1103 20-21 0.41457798128146095 1103 22-23 0.5087892831836882 1103 24-25 0.3454658236606747 1103 26-27 0.5430147628008442 1103 28-29 0.4598938458813535 1103 30-31 0.39664742707865486 1103 32-33 0.49855669257888735 1103 34-35 0.45709073747758566 1103 36-37 0.3487867170975676 1103 38-39 0.4800354200002559 1103 40-41 0.5878062291313881 1103 42-43 0.5772942316280592 1103 44-45 0.7031413566221758 1103 46-47 0.5147067174625093 1103 48-49 0.5640354810749315 1103 50-51 0.3603771525177919 1103 52-53 0.4556179412288941 1103 54-55 0.5599367372007151 1103 56-57 0.6040641050674935 1103 58-59 0.5348988075467886 1103 60-61 0.671172136518301 1103 62-63 0.6289886499381367 1103 64-65 0.6383887984577896 1103 66-67 0.7167546695771705 1103 68-69 0.648300379381773 1103 70-71 0.464047693767462 1103 72-73 0.5397740495309051 1103 74-75 0.4130987133606716 1103 76-77 0.5409627349074171 1103 78-79 0.4045761026848673 1103 80-81 0.47783622025036365 1103 82-83 0.628566326212102 1103 84-85 0.538118050554079 1103 86-87 0.5710048949615718 1103 88-89 0.5107533135267239 1103 90-91 0.6156873265564293 1103 92-93 0.540663586786291 1103 94-95 0.39726116827865 1103 96-97 0.47045444352027843 1103 98-99 0.5611317006564711 1103 100-101 0.652602752236934 1104 1 0.3112753945025375 1104 2 0.2350455133850602 1104 3 0.3337936148696947 1104 4 0.23643263074414023 1104 5 0.21311392614533275 1104 6 0.16768818258591267 1104 7 0.1390763308870291 1104 8 0.21036508577601865 1104 9 0.28706195391011846 1104 10-11 0.414809487621163 1104 12-13 0.39435496778589396 1104 14-15 0.4352009441933191 1104 16-17 0.47387414042932363 1104 18-19 0.4051285738184376 1104 20-21 0.4186554384100276 1104 22-23 0.29778336677193806 1104 24-25 0.492850441756282 1104 26-27 0.27115239651578804 1104 28-29 0.26778907316109724 1104 30-31 0.4587763584642559 1104 32-33 0.41968419528388523 1104 34-35 0.43523378213596686 1104 36-37 0.4141921902141874 1104 38-39 0.5015120769987291 1104 40-41 0.5137987924839607 1104 42-43 0.5343662006234524 1104 44-45 0.41577093914059304 1104 46-47 0.46254445658071575 1104 48-49 0.43020321725686017 1104 50-51 0.33412880955936686 1104 52-53 0.39020241237668785 1104 54-55 0.395050350732177 1104 56-57 0.3282238813206675 1104 58-59 0.33823237008704865 1104 60-61 0.37599894706306713 1104 62-63 0.49253394604698997 1104 64-65 0.4944813579604457 1104 66-67 0.47083456881295405 1104 68-69 0.4264005578758727 1104 70-71 0.3213613068775416 1104 72-73 0.44793405869407366 1104 74-75 0.4091774553249401 1104 76-77 0.39523932433134945 1104 78-79 0.47535191322025483 1104 80-81 0.45971884932147944 1104 82-83 0.4519641458604724 1104 84-85 0.45846101434519326 1104 86-87 0.4237248565160989 1104 88-89 0.5045126282503709 1104 90-91 0.5065385417010937 1104 92-93 0.5222568022102188 1104 94-95 0.4729037633019999 1104 96-97 0.5775236064865936 1104 98-99 0.6174008197223202 1104 100-101 0.5176527694657622 1105 1 -0.0209248724598865 1105 2 -0.0010547846043813536 1105 3 0.1332117913457047 1105 4 0.011637682600579069 1105 5 -0.050126786504321785 1105 6 0.0165477771075615 1105 7 -0.01943251296061277 1105 8 0.030787328665915936 1105 9 0.025624519259103806 1105 10-11 0.0405153905207527 1105 12-13 -0.03094127136468927 1105 14-15 -2.9576162503275327E-4 1105 16-17 -0.06692523948942863 1105 18-19 -0.12970422652099245 1105 20-21 -0.0298150367624217 1105 22-23 -0.07153196528027195 1105 24-25 -0.04469827467782039 1105 26-27 -0.15910969569986833 1105 28-29 -0.0846457060210426 1105 30-31 -0.42663409530683083 1105 32-33 -0.3312964459232859 1105 34-35 -0.13438983068665067 1105 36-37 -0.30218806634978534 1105 38-39 -0.39659755128536034 1105 40-41 -0.3656747314468163 1105 42-43 -0.2611591564072313 1105 44-45 -0.06984329441748116 1105 46-47 -0.030456196478695574 1105 48-49 0.052919470019553216 1105 50-51 -0.15633631259181868 1105 52-53 -0.3015265729403822 1105 54-55 -0.12952201395120255 1105 56-57 -7.411208925418578E-4 1105 58-59 -0.1542511341371835 1105 60-61 -0.004342549066585377 1105 62-63 0.023750243379922154 1105 64-65 -0.06966489597675007 1105 66-67 -0.03555810620945721 1105 68-69 0.05441655802277978 1105 70-71 -0.007440813059680806 1105 72-73 -0.02868138342561366 1105 74-75 -0.08135815668967084 1105 76-77 -0.10771264194369934 1105 78-79 -0.16466171035660793 1105 80-81 -0.104193554254703 1105 82-83 -0.08844519652362237 1105 84-85 -0.16923658586231838 1105 86-87 -0.06962881972634705 1105 88-89 -0.13870651469235895 1105 90-91 -0.25420135892828455 1105 92-93 -0.25907159026509774 1105 94-95 -0.21093418308438316 1105 96-97 -0.23481535239055518 1105 98-99 -0.10340509079055948 1105 100-101 -0.14732383019504525 1106 1 0.25623370032646164 1106 2 0.24144153550022196 1106 3 0.24740551162075164 1106 4 0.15691126085717144 1106 5 0.11104223926345469 1106 6 0.1714663659172757 1106 7 0.22602567416891617 1106 8 0.3215323208904266 1106 9 0.15586825209963706 1106 10-11 0.37685159407801194 1106 12-13 0.30429823833530634 1106 14-15 0.43317702037588646 1106 16-17 0.4636251670468994 1106 18-19 0.32612087310544524 1106 20-21 0.4109704862740031 1106 22-23 0.28464496824730645 1106 24-25 0.34041822542516087 1106 26-27 0.26731280353013886 1106 28-29 0.32840011363668964 1106 30-31 0.34645098916542594 1106 32-33 0.41767516739198385 1106 34-35 0.40416895410702836 1106 36-37 0.3103964200804228 1106 38-39 0.26218865954666626 1106 40-41 0.1866176686007961 1106 42-43 0.23819301194397013 1106 44-45 0.3693753206569639 1106 46-47 0.3604823799733765 1106 48-49 0.4304215626943346 1106 50-51 0.4424875380431672 1106 52-53 0.35341053344308193 1106 54-55 0.490312197077337 1106 56-57 0.42831200753817456 1106 58-59 0.5226893847705796 1106 60-61 0.5599731415538827 1106 62-63 0.5583914275976625 1106 64-65 0.39200892263038867 1106 66-67 0.5520729103486559 1106 68-69 0.26332851829690895 1106 70-71 0.23369474880697538 1106 72-73 0.4473798341615449 1106 74-75 0.38350399131109114 1106 76-77 0.5937969182261327 1106 78-79 0.5683087352177871 1106 80-81 0.5922567402314058 1106 82-83 0.501177592941012 1106 84-85 0.563067699444364 1106 86-87 0.7121684052829558 1106 88-89 0.5956821313895695 1106 90-91 0.5043883388762502 1106 92-93 0.6668821120214474 1106 94-95 0.573584747355671 1106 96-97 0.5415244790995253 1106 98-99 0.5455724746019861 1106 100-101 0.6116507686066015 1107 1 0.1794201938194 1107 2 0.23854667163318766 1107 3 0.1955266363181316 1107 4 0.24140315475378316 1107 5 0.1695927386381868 1107 6 0.14353194530938396 1107 7 0.18775897693779342 1107 8 0.23720648143954293 1107 9 0.32727706845385995 1107 10-11 0.47705669275088525 1107 12-13 0.4355028692229226 1107 14-15 0.339243039597946 1107 16-17 0.32415797631166043 1107 18-19 0.2797251491472039 1107 20-21 0.4275576667216967 1107 22-23 0.306019399590042 1107 24-25 0.408604635052221 1107 26-27 0.35667814381129404 1107 28-29 0.3242669234344575 1107 30-31 0.23809999740478105 1107 32-33 0.46833901243765297 1107 34-35 0.3791898739852897 1107 36-37 0.4179805049452341 1107 38-39 0.3193500603678032 1107 40-41 0.4841637529027807 1107 42-43 0.3756157388524315 1107 44-45 0.41377848445075216 1107 46-47 0.36720190078236215 1107 48-49 0.48851890675504706 1107 50-51 0.3213204417968214 1107 52-53 0.33662254080091714 1107 54-55 0.3239493112709937 1107 56-57 0.5224659032947443 1107 58-59 0.5917434335357967 1107 60-61 0.6108940821190671 1107 62-63 0.5130360133095309 1107 64-65 0.48847239158417466 1107 66-67 0.5189126202845316 1107 68-69 0.5475927629169135 1107 70-71 0.5606634071951433 1107 72-73 0.50761864691885 1107 74-75 0.5764699734457999 1107 76-77 0.5972658906454384 1107 78-79 0.6014418154625254 1107 80-81 0.6089136225452094 1107 82-83 0.6159487043130412 1107 84-85 0.6321310666408806 1107 86-87 0.4101056773308329 1107 88-89 0.403054035573728 1107 90-91 0.44856217607580007 1107 92-93 0.4218118417310279 1107 94-95 0.41901098172167295 1107 96-97 0.39078161375644527 1107 98-99 0.44946012413950953 1107 100-101 0.46175951469984966 1108 1 0.13880113738146704 1108 2 0.1664460275605073 1108 3 0.08933327929139168 1108 4 0.0980613405912365 1108 5 0.12566108794840147 1108 6 0.004057947796304973 1108 7 0.08509795141887366 1108 8 0.13967069880745697 1108 9 0.02441372297985822 1108 10-11 -0.0399327084173251 1108 12-13 0.2073536815893533 1108 14-15 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50-51 -1.2668953195187171 2316 52-53 -1.0144313301993364 2316 54-55 -1.154909054497793 2316 56-57 -1.4064705424270016 2316 58-59 -1.349203724406955 2316 60-61 -1.34762636907017 2316 62-63 -1.322872960846425 2316 64-65 -1.5086097442832838 2316 66-67 -1.3423689289793401 2316 68-69 -1.409962959498813 2316 70-71 -1.3068613181232962 2316 72-73 -1.6389799690846267 2316 74-75 -1.7300531311737615 2316 76-77 -2.0245017091669553 2316 78-79 -2.117817186462762 2316 80-81 -1.988767012641457 2316 82-83 -1.6391800991959755 2316 84-85 -1.858517523563247 2316 86-87 -2.484123452666797 2316 88-89 -2.580559592541416 2316 90-91 -2.158249949388569 2316 92-93 -2.2541372218020364 2316 94-95 -2.858949104685294 2316 96-97 -2.8077642276241868 2316 98-99 -2.793019045068572 2316 100-101 -3.0066264389401276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4095.0 3 1624.0 4 1643.0 5 1743.0 6 2015.0 7 2324.0 8 2942.0 9 3634.0 10 4635.0 11 5796.0 12 7526.0 13 9532.0 14 10616.0 15 13467.0 16 17143.0 17 23113.0 18 19954.0 19 12412.0 20 11792.0 21 12263.0 22 13479.0 23 14787.0 24 16748.0 25 19027.0 26 22413.0 27 26451.0 28 31996.0 29 38162.0 30 46838.0 31 58059.0 32 73908.0 33 96067.0 34 130879.0 35 186045.0 36 279269.0 37 405982.0 38 357696.0 39 61134.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.308597453113244 19.029622462642077 14.84661017777387 21.815169906470814 2 15.371508207904256 20.68604925035745 40.51265562376065 23.42978691797764 3 18.901283703695743 26.177265829365222 30.078847568858485 24.84260289808055 4 12.712288066225716 16.966088152721817 37.67335260355709 32.648271177495374 5 13.574849948190582 38.05211245576648 33.287552053803594 15.08548554223934 6 30.539991006666796 36.29161860446929 18.142290171850867 15.026100217013042 7 27.900827383419486 33.86206431381136 20.757558280481415 17.47955002228774 8 26.901758718074298 33.79136643527955 19.980869260900462 19.326005585745694 9 27.507222844864877 16.521949090368775 20.308327863147436 35.66250020161891 10-11 24.730183628374277 27.067056508013167 27.061044159235347 21.14171570437721 12-13 26.148588489271578 24.689089304476553 27.710956740978915 21.451365465272954 14-15 23.34469746958566 25.307760360915193 26.685582454751188 24.66195971474796 16-17 21.780983498877273 28.870867907690283 26.91029202151277 22.437856571919674 18-19 21.77808022362531 26.92959907735012 30.010800086009738 21.281520613014838 20-21 23.117695988927423 25.881368589380177 29.773118697877365 21.227816723815035 22-23 22.719058460460502 26.07346652786773 29.732378809692438 21.475096201979326 24-25 22.205310283590492 26.19643823335788 29.98313284502859 21.615118638023038 26-27 21.97729321214579 26.416352990891394 29.717972705932933 21.888381091029892 28-29 21.795163356001005 26.354387097136662 30.003305006682073 21.847144540180256 30-31 21.9928484697782 26.129123738473538 30.019719346772515 21.858308444975744 32-33 21.83492763832155 26.358375206025368 29.760981262072082 22.045715893580997 34-35 22.22431740064132 26.362292064397753 29.643292617394586 21.770097917566346 36-37 22.45481010682613 26.01836783194272 29.487231154727038 22.039590906504117 38-39 22.139189680006233 26.140351348558944 29.679874336577587 22.04058463485724 40-41 22.1819617118883 26.15900809175195 29.681823100121175 21.977207096238576 42-43 22.1651016727056 26.249766441595746 29.289750419183907 22.295381466514748 44-45 22.149032825322394 26.4322245017585 29.102041813208285 22.316700859710824 46-47 22.436485018741685 26.56817970329349 28.66797627657046 22.327359001394363 48-49 22.53644173169634 26.778075392003686 28.190390173145698 22.495092703154274 50-51 22.46668049102357 26.965971321054084 28.014277678572956 22.553070509349393 52-53 22.6405490435726 26.978904790062742 27.98333857239194 22.397207593972713 54-55 22.356234835039917 26.945303802359565 27.967251746519782 22.731209616080733 56-57 22.42412264962391 27.28142610796945 27.794013358097512 22.500437884309132 58-59 22.70343242798965 26.85789962577847 27.77524432115589 22.663423625075993 60-61 22.573213555057496 26.84954228169066 28.243448734938653 22.33379542831319 62-63 22.52731321769942 26.766306040430425 28.28581178275858 22.420568959111577 64-65 22.490878207893324 26.900676641812666 28.06387916880188 22.54456598149213 66-67 22.37119000166349 27.20552366859063 27.83476773785536 22.58851859189052 68-69 22.317128107309987 27.099886353975606 28.057791397494313 22.525194141220087 70-71 22.395816412052536 27.019787180873294 27.89974670903735 22.684649698036818 72-73 22.353384608394578 27.161704046521784 27.606603060576912 22.878308284506726 74-75 22.358379350995843 27.429661740256655 27.542102313837937 22.669856594909565 76-77 22.34023997349914 27.26830557680901 27.578703744419137 22.812750705272713 78-79 22.413333525533226 27.315481383770816 27.488974363547563 22.78221072714839 80-81 22.36209926810284 27.5191671646473 27.436029236797243 22.68270433045262 82-83 22.44023293130305 27.411473205473243 27.569270579865652 22.579023283358058 84-85 22.423577969818133 27.3175779463668 27.343545411551347 22.915298672263717 86-87 22.474891272669797 27.04809604111106 27.546740598564014 22.930272087655126 88-89 22.64132368848158 27.047340614199744 27.369107095571387 22.942228601747292 90-91 22.40572818714319 27.096301873039224 27.676702931147485 22.821267008670105 92-93 22.499083850855804 27.190915709392605 27.36302959528195 22.946970844469636 94-95 22.345652774011672 27.038814910358543 27.38311432919754 23.232417986432246 96-97 22.38972400889528 27.026639413353376 27.397960106113967 23.185676471637386 98-99 22.259538962855807 27.17438503333704 27.277847760242114 23.28822824356504 100-101 22.299792663960186 27.08948979302565 27.368259501435517 23.242458041578647 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 436.0 1 343.0 2 289.5 3 357.5 4 359.5 5 406.5 6 646.5 7 1310.0 8 2379.5 9 3060.0 10 2887.0 11 2667.0 12 2791.5 13 2980.5 14 3229.0 15 3396.5 16 3518.5 17 3708.5 18 3927.0 19 4029.0 20 4288.5 21 4824.5 22 5550.5 23 6451.0 24 7402.5 25 9132.5 26 11550.5 27 13881.5 28 15986.0 29 18397.0 30 22078.5 31 26161.0 32 30382.0 33 34522.0 34 39161.5 35 42652.5 36 46818.0 37 52178.0 38 54917.5 39 56436.0 40 58282.0 41 62295.5 42 64940.5 43 67669.5 44 73269.5 45 77586.0 46 78100.0 47 77775.5 48 80303.5 49 79024.0 50 74230.5 51 70920.0 52 68258.5 53 67890.5 54 67545.0 55 64051.0 56 58916.5 57 53162.0 58 48766.0 59 44296.0 60 37739.5 61 30759.0 62 24902.0 63 19658.5 64 14889.0 65 11287.0 66 8091.5 67 6050.5 68 4182.0 69 2413.0 70 1553.5 71 1028.0 72 681.0 73 462.0 74 326.0 75 243.5 76 156.5 77 71.0 78 36.5 79 25.5 80 20.0 81 17.0 82 15.5 83 13.0 84 10.0 85 4.0 86 2.5 87 4.5 88 5.5 89 4.5 90 3.5 91 3.5 92 3.0 93 2.5 94 2.0 95 2.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0395168298221645 2 0.0384422065142688 3 0.024179024427653184 4 0.04366878351176151 5 0.061986226259983616 6 0.061986226259983616 7 0.06159545414802154 8 0.06384239379180345 9 0.06301200305388405 10-11 0.07031455689617527 12-13 0.06958185918624639 14-15 0.06442855195974656 16-17 0.05351135608180618 18-19 0.04635534178150075 20-21 0.05338923979681805 22-23 0.03045580147604396 24-25 0.017169549669333522 26-27 0.012895479694748364 28-29 0.016339158931414122 30-31 0.012431437811793403 32-33 0.007375823613284103 34-35 0.00981814931304705 36-37 0.005739465394442927 38-39 0.008474870178177428 40-41 0.007058321272314919 42-43 0.00471368860054249 44-45 0.0014165489058625097 46-47 0.0026621350127416133 48-49 0.0010990465648933266 50-51 0.005739465394442927 52-53 0.00515330722649982 54-55 0.007717749211250915 56-57 0.022249587124840455 58-59 0.008767949262148982 60-61 0.01912341022914388 62-63 0.013701447175670138 64-65 0.010160074911013863 66-67 0.01604607984744257 68-69 0.025546726819520433 70-71 0.01824417297722922 72-73 0.017584745038293225 74-75 0.018073210178245813 76-77 0.024569796539615257 78-79 0.03656161572545133 80-81 0.030773303817013143 82-83 0.030138299135074775 84-85 0.021345926615928164 86-87 0.023470749974721927 88-89 0.01968514514008936 90-91 0.017999940407252925 92-93 0.030235992163065297 94-95 0.022664782493800155 96-97 0.03663488549644422 98-99 0.05282750488587256 100-101 0.06227930534395517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2047229.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.499911640084584 #Duplication Level Percentage of deduplicated Percentage of total 1 72.63540590867086 35.22810768512215 2 14.105308271683187 13.682124096655773 3 5.283732399716964 7.6878166354837445 4 2.5503157988908214 4.947603636020662 5 1.3742178914594112 3.3324723155002394 6 0.8578885145554039 2.49645102917883 7 0.5921974361030928 2.0105066327139243 8 0.42368875763001124 1.6439093846362205 9 0.300809703447436 1.3130319633912626 >10 1.6656560575483699 14.92396877649042 >50 0.1397376360582804 4.715135537769901 >100 0.06791875822917738 5.778700042968472 >500 0.0023169651020159224 0.714054603811486 >1k 5.036880656556348E-4 0.3223139391956462 >5k 2.0147522626225392E-4 0.6546436973294624 >10k+ 1.0073761313112696E-4 0.5491600237318573 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11240 0.5490348173067107 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8291 0.404986447534692 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5108 0.24950799348778274 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2062 0.10072151185822396 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 2.442325699762948E-5 0.0 0.0 0.0 12-13 0.0 4.884651399525896E-5 0.0 2.442325699762948E-5 0.0 14-15 0.0 4.884651399525896E-5 0.0 4.884651399525896E-5 0.0 16-17 0.0 4.884651399525896E-5 0.0 7.326977099288844E-5 0.0 18-19 0.0 4.884651399525896E-5 0.0 9.769302799051792E-5 0.0 20-21 0.0 4.884651399525896E-5 0.0 9.769302799051792E-5 0.0 22-23 0.0 4.884651399525896E-5 0.0 1.9538605598103584E-4 0.0 24-25 0.0 4.884651399525896E-5 0.0 3.9077211196207167E-4 0.0 26-27 0.0 4.884651399525896E-5 0.0 5.128883969502191E-4 0.0 28-29 0.0 4.884651399525896E-5 0.0 0.001123469821890956 0.0 30-31 0.0 4.884651399525896E-5 0.0 0.0022713629007795415 0.0 32-33 0.0 4.884651399525896E-5 0.0 0.003565795521653904 0.0 34-35 0.0 4.884651399525896E-5 0.0 0.005080037455506932 0.0 36-37 0.0 4.884651399525896E-5 0.0 0.007449093384276991 0.0 38-39 0.0 4.884651399525896E-5 0.0 0.011112581933921412 0.0 40-41 0.0 4.884651399525896E-5 0.0 0.015728577506473385 0.0 42-43 0.0 4.884651399525896E-5 0.0 0.021516889414911568 0.0 44-45 0.0 4.884651399525896E-5 0.0 0.03201888992389225 4.884651399525896E-5 46-47 0.0 4.884651399525896E-5 0.0 0.0550988677866521 4.884651399525896E-5 48-49 0.0 4.884651399525896E-5 0.0 0.0723172639699809 4.884651399525896E-5 50-51 0.0 4.884651399525896E-5 0.0 0.09356549755791853 4.884651399525896E-5 52-53 0.0 4.884651399525896E-5 0.0 0.11815971735453142 4.884651399525896E-5 54-55 0.0 4.884651399525896E-5 0.0 0.14653954198577687 4.884651399525896E-5 56-57 0.0 4.884651399525896E-5 0.0 0.1764824550648706 4.884651399525896E-5 58-59 0.0 4.884651399525896E-5 0.0 0.22571974117209165 4.884651399525896E-5 60-61 0.0 4.884651399525896E-5 0.0 0.28399363236843556 4.884651399525896E-5 62-63 0.0 4.884651399525896E-5 0.0 0.3502295053460067 4.884651399525896E-5 64-65 0.0 4.884651399525896E-5 0.0 0.40300816371788406 4.884651399525896E-5 66-67 0.0 4.884651399525896E-5 0.0 0.4840933769500139 4.884651399525896E-5 68-69 0.0 4.884651399525896E-5 0.0 0.5867687493680482 4.884651399525896E-5 70-71 0.0 4.884651399525896E-5 0.0 0.6586219714550741 4.884651399525896E-5 72-73 0.0 4.884651399525896E-5 0.0 0.7794194005653496 4.884651399525896E-5 74-75 0.0 4.884651399525896E-5 0.0 0.8761599215329599 4.884651399525896E-5 76-77 0.0 4.884651399525896E-5 0.0 0.9599561162918266 4.884651399525896E-5 78-79 0.0 9.769302799051792E-5 0.0 1.0642434236717047 4.884651399525896E-5 80-81 0.0 9.769302799051792E-5 0.0 1.1620097214332152 4.884651399525896E-5 82-83 0.0 9.769302799051792E-5 0.0 1.302834221281547 4.884651399525896E-5 84-85 0.0 9.769302799051792E-5 0.0 1.5022989611811868 4.884651399525896E-5 86-87 0.0 9.769302799051792E-5 0.0 1.7648733971627015 4.884651399525896E-5 88-89 0.0 9.769302799051792E-5 0.0 2.083816710294745 4.884651399525896E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6215 0.0 47.947998 1 GTATCAA 11215 0.0 43.692158 1 ATCAACG 14675 0.0 33.261898 3 TATCAAC 14700 0.0 33.206146 2 TCAACGC 14990 0.0 32.562935 4 CAACGCA 15155 0.0 32.18019 5 AACGCAG 15635 0.0 31.249992 6 ACGCAGA 17610 0.0 27.743881 7 CGCAGAG 17780 0.0 27.266108 8 GTACATG 13325 0.0 25.502508 1 GCAGAGT 18570 0.0 25.389519 9 TACATGG 13660 0.0 24.878298 2 ACATGGG 13680 0.0 23.799026 3 GAGTACT 9025 0.0 23.512405 12-13 GTATACG 105 0.002189452 22.632097 1 AGTACTT 9445 0.0 22.542334 12-13 GTACTTT 9650 0.0 21.816944 14-15 CATGGGG 5505 0.0 21.756947 4 AGAGTAC 17260 0.0 21.189072 10-11 CAGAGTA 18230 0.0 20.609112 10-11 >>END_MODULE