FastQCFastQC Report
Thu 26 May 2016
SRR937130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047229
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111590.5450782496730947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88270.43116817903615084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55970.2733939388314644No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.11688970799065468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67300.047.018111
GTATCAA119150.042.8268551
ATCAACG156600.032.3919373
TCAACGC158850.031.9001124
TATCAAC160000.031.8840562
CAACGCA161200.031.4056055
AACGCAG166100.030.5348226
ACGCAGA190050.026.4625997
CGCAGAG190750.026.2659028
TATCACG1151.2823186E-424.782362
GCAGAGT200450.024.568419
AGTACTT104000.022.94656812-13
GTACATG132950.022.6214961
GAGTACT99450.022.53991112-13
GTATAGG5400.021.9964311
TACATGG138600.021.4878792
GTACTTT104500.021.33756614-15
AGAGTAC188150.020.97496210-11
ACATGGG137050.020.8282533
TACTTTT118150.019.91753214-15
Produced by FastQC (version 0.11.5)