##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937130_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2047229 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12708397546147 33.0 31.0 34.0 31.0 34.0 2 32.196292158815645 33.0 31.0 34.0 30.0 34.0 3 32.329238692886825 34.0 31.0 34.0 30.0 34.0 4 36.02440127606633 37.0 35.0 37.0 35.0 37.0 5 35.93160022645244 37.0 35.0 37.0 35.0 37.0 6 35.96425460952341 37.0 35.0 37.0 35.0 37.0 7 35.91163665618258 37.0 35.0 37.0 35.0 37.0 8 35.89792543970411 37.0 35.0 37.0 35.0 37.0 9 37.5649959042198 39.0 37.0 39.0 35.0 39.0 10-11 37.46475894978041 39.0 37.0 39.0 34.5 39.0 12-13 37.38951358152898 39.0 37.0 39.0 34.0 39.0 14-15 38.58279215466369 40.0 38.0 41.0 34.0 41.0 16-17 38.503967802331836 40.0 38.0 41.0 34.0 41.0 18-19 38.55765329623604 40.0 38.0 41.0 34.0 41.0 20-21 38.65190264498989 40.0 38.0 41.0 34.0 41.0 22-23 38.581788114568525 40.0 38.0 41.0 34.0 41.0 24-25 38.492265398741424 40.0 38.0 41.0 34.0 41.0 26-27 38.31468462980936 40.0 38.0 41.0 34.0 41.0 28-29 38.15499756011663 40.0 38.0 41.0 33.5 41.0 30-31 38.094918301763016 40.0 37.5 41.0 33.5 41.0 32-33 37.99966882063511 40.0 37.0 41.0 33.0 41.0 34-35 37.87265493992123 40.0 37.0 41.0 33.0 41.0 36-37 37.70044679906351 40.0 37.0 41.0 33.0 41.0 38-39 37.552665334459405 40.0 37.0 41.0 33.0 41.0 40-41 37.31436004472387 40.0 36.0 41.0 32.0 41.0 42-43 37.0721292048911 40.0 36.0 41.0 31.5 41.0 44-45 36.79481533331152 39.5 36.0 41.0 31.0 41.0 46-47 36.38781421130709 39.0 35.5 41.0 30.0 41.0 48-49 35.98264336818207 39.0 35.0 41.0 29.0 41.0 50-51 35.734671597559434 39.0 35.0 40.0 28.0 41.0 52-53 35.50316305601376 39.0 35.0 40.0 28.0 41.0 54-55 35.22621406789372 38.0 35.0 40.0 27.0 41.0 56-57 34.972103755857304 38.0 34.0 40.0 26.5 41.0 58-59 34.61470407072194 38.0 34.0 40.0 26.0 41.0 60-61 34.28118569051142 37.5 34.0 40.0 26.0 41.0 62-63 33.94280415136753 37.0 33.0 40.0 25.0 41.0 64-65 33.5302120573712 36.0 33.0 39.0 23.5 41.0 66-67 33.10377979209947 36.0 32.5 39.0 23.0 40.0 68-69 32.66659250137625 35.0 32.0 38.5 22.0 40.0 70-71 32.27966119080963 35.0 32.0 38.0 21.0 40.0 72-73 31.90738920755812 35.0 31.0 37.0 20.0 39.5 74-75 31.43862606479295 35.0 31.0 37.0 18.5 39.0 76-77 29.96665517145371 33.5 29.5 35.0 17.5 37.5 78-79 30.692512659795263 34.0 30.5 36.0 18.0 38.0 80-81 30.422589998480873 34.0 30.0 35.5 12.0 37.0 82-83 30.233378874566547 34.0 30.0 35.0 12.5 37.0 84-85 29.918160352359212 34.0 30.0 35.0 8.5 36.0 86-87 29.563023970449812 34.0 30.0 35.0 7.0 36.0 88-89 29.206155002689 34.0 29.0 35.0 2.0 35.5 90-91 28.77448761227982 34.0 29.0 35.0 2.0 35.0 92-93 28.361590716036165 34.0 29.0 35.0 2.0 35.0 94-95 27.889613961115245 33.0 28.0 35.0 2.0 35.0 96-97 27.282982265296162 33.0 27.0 35.0 2.0 35.0 98-99 26.532096800113713 33.0 25.5 34.5 2.0 35.0 100-101 23.430834557345563 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31364294297697626 1101 2 0.2690559836815467 1101 3 0.1879468417327459 1101 4 0.129906927443578 1101 5 0.16753556742008158 1101 6 0.17511919422831568 1101 7 0.20960222911929094 1101 8 0.17992159225133975 1101 9 0.2981596562637492 1101 10-11 0.2408216204983873 1101 12-13 0.18046286406994483 1101 14-15 0.3127063801636396 1101 16-17 0.30115850927556664 1101 18-19 0.25079702595653686 1101 20-21 0.2832977220932946 1101 22-23 0.24146915755322595 1101 24-25 0.21204498959282603 1101 26-27 0.24839897214274487 1101 28-29 0.178196104005103 1101 30-31 0.20460691369080308 1101 32-33 0.3098434516752917 1101 34-35 0.3501035589207575 1101 36-37 0.4065428714530128 1101 38-39 0.33769257568648214 1101 40-41 0.3173241333373653 1101 42-43 0.518256959157469 1101 44-45 0.35860646140447017 1101 46-47 0.364862967933945 1101 48-49 0.4793244547029971 1101 50-51 0.5152571079019239 1101 52-53 0.48255088453517914 1101 54-55 0.5407399979363348 1101 56-57 0.5057863055327445 1101 58-59 0.5211901772141516 1101 60-61 0.3998439064690231 1101 62-63 0.41196185651760686 1101 64-65 0.5514397386744605 1101 66-67 0.6251882291802744 1101 68-69 0.5731079255889924 1101 70-71 0.5701621721553138 1101 72-73 0.5705968754236608 1101 74-75 0.4022190687567502 1101 76-77 0.41940449165998217 1101 78-79 0.6248348755297073 1101 80-81 0.8214772005667932 1101 82-83 0.5939676981939179 1101 84-85 0.4970964094497923 1101 86-87 0.3981865050574669 1101 88-89 0.32108956426423774 1101 90-91 0.11065503306462787 1101 92-93 0.4509964395690744 1101 94-95 0.39529750452614465 1101 96-97 0.41641123737850094 1101 98-99 0.5358596291487459 1101 100-101 0.9578839738579852 1102 1 0.2779967962303971 1102 2 0.2296598010449742 1102 3 0.20139404100271463 1102 4 0.17083857750514397 1102 5 0.1804428563962972 1102 6 0.24243133977564923 1102 7 0.19728249569973855 1102 8 0.22453045793206172 1102 9 0.2840121340462787 1102 10-11 0.2875853420220196 1102 12-13 0.25818997280975253 1102 14-15 0.3491463718912584 1102 16-17 0.4150292312407018 1102 18-19 0.31833793118323683 1102 20-21 0.2927217054286899 1102 22-23 0.29449396596309185 1102 24-25 0.22078363015072 1102 26-27 0.25262067532981547 1102 28-29 0.29035575766356914 1102 30-31 0.40584521199343015 1102 32-33 0.3101722872125805 1102 34-35 0.3458655231872214 1102 36-37 0.3434110255673488 1102 38-39 0.3183570252879875 1102 40-41 0.37345410212451213 1102 42-43 0.25053541804751944 1102 44-45 0.30617784295480277 1102 46-47 0.44454571706145174 1102 48-49 0.594818860649788 1102 50-51 0.5029646300694637 1102 52-53 0.46543042274647206 1102 54-55 0.4356871865953522 1102 56-57 0.4983611346027672 1102 58-59 0.6282744660534618 1102 60-61 0.5954944973327017 1102 62-63 0.49211554473949093 1102 64-65 0.5890498481253985 1102 66-67 0.4479903989746745 1102 68-69 0.47766510136089835 1102 70-71 0.31183246943263043 1102 72-73 0.3025741704793319 1102 74-75 0.3637821371449732 1102 76-77 0.3781772953277951 1102 78-79 0.36058342838286706 1102 80-81 0.3423007811852834 1102 82-83 0.2698433203391062 1102 84-85 0.3229110498196057 1102 86-87 0.25302751343928875 1102 88-89 0.19404940034788964 1102 90-91 0.21304626350666211 1102 92-93 0.3999011602783682 1102 94-95 0.5204279048254143 1102 96-97 0.5316000062457142 1102 98-99 0.5843122608109184 1102 100-101 0.9075665190887392 1103 1 0.20521557957567182 1103 2 0.14090846723404837 1103 3 0.17265906221355465 1103 4 0.106838939123719 1103 5 0.13981007868624573 1103 6 0.15743179864769985 1103 7 0.1816466017690459 1103 8 0.0837978241845505 1103 9 0.17009954437789077 1103 10-11 0.16112910814246817 1103 12-13 0.2199296320938302 1103 14-15 0.25132923308862587 1103 16-17 0.17124626265356824 1103 18-19 0.3385180281901583 1103 20-21 0.28048694374766114 1103 22-23 0.31567262334456814 1103 24-25 0.25320191325696584 1103 26-27 0.12308588940390308 1103 28-29 0.15538231060302365 1103 30-31 0.2660734700192293 1103 32-33 0.21962861286654345 1103 34-35 0.31090632170677424 1103 36-37 0.29184202559336825 1103 38-39 0.21092138038098796 1103 40-41 0.2809948081847722 1103 42-43 0.18932462592477606 1103 44-45 0.1490196444179901 1103 46-47 0.19434538859125183 1103 48-49 0.1489157662084395 1103 50-51 0.030860626376565392 1103 52-53 0.05062721036815532 1103 54-55 0.04929020884996049 1103 56-57 0.03101775722227984 1103 58-59 0.057524614330773716 1103 60-61 -0.010798761631399145 1103 62-63 0.0745714279586025 1103 64-65 0.016797361655278564 1103 66-67 0.023113232858882782 1103 68-69 -0.10548525388825425 1103 70-71 0.17864245941962764 1103 72-73 0.049322685309114433 1103 74-75 -0.10808695145294678 1103 76-77 0.022664461292912108 1103 78-79 0.03870249129764858 1103 80-81 0.34744768188125974 1103 82-83 0.2494721407913758 1103 84-85 0.22200508571572541 1103 86-87 0.19447543968005832 1103 88-89 -0.029864408413416754 1103 90-91 0.13285529054724066 1103 92-93 0.15701059509867576 1103 94-95 0.3838032032634686 1103 96-97 0.7111189713591095 1103 98-99 0.8179178582280819 1103 100-101 0.9550029310654331 1104 1 0.15307017402210477 1104 2 0.12060584130799157 1104 3 0.08924414973006378 1104 4 0.09392354386914548 1104 5 -0.06678816939391652 1104 6 0.06107046178266273 1104 7 0.08870364039130152 1104 8 0.0892081930078632 1104 9 0.14579531030028647 1104 10-11 0.127057594432479 1104 12-13 0.12964033145395604 1104 14-15 0.14317299722052468 1104 16-17 0.10415717209238551 1104 18-19 0.12257254291855446 1104 20-21 0.18803920340552338 1104 22-23 0.13825209765369095 1104 24-25 0.17800445212908755 1104 26-27 0.20121387918957367 1104 28-29 0.11530532006058536 1104 30-31 0.31446722427735807 1104 32-33 0.2963662296112375 1104 34-35 0.1841638303245432 1104 36-37 0.22295267105475602 1104 38-39 0.23361966560034375 1104 40-41 0.3103603334073384 1104 42-43 0.2708975191859153 1104 44-45 0.3449174406029769 1104 46-47 0.3795230044855131 1104 48-49 0.6834092377375853 1104 50-51 0.5963843740975037 1104 52-53 0.5130914478627986 1104 54-55 0.3662443131319577 1104 56-57 0.41302701194670277 1104 58-59 0.4592816099144841 1104 60-61 0.45782523641032213 1104 62-63 0.3229860215316336 1104 64-65 0.3514425694460428 1104 66-67 0.2685544890711711 1104 68-69 0.4837158246229052 1104 70-71 0.34939335088453305 1104 72-73 0.49720189236552415 1104 74-75 0.5587309024002494 1104 76-77 0.537703149059638 1104 78-79 0.6819857100370967 1104 80-81 0.7010450781927418 1104 82-83 0.5844718850883837 1104 84-85 0.446545447618778 1104 86-87 0.5827343819584101 1104 88-89 0.41645194579441025 1104 90-91 0.4696018597696785 1104 92-93 0.38294937463117407 1104 94-95 0.5367120594168426 1104 96-97 0.7009697653121094 1104 98-99 0.7298459909392783 1104 100-101 0.5848608808714175 1105 1 0.007911938147145747 1105 2 -0.019541588671764032 1105 3 -0.04004325035509737 1105 4 -0.013886732998209084 1105 5 0.036939242765377855 1105 6 -0.011422958844597986 1105 7 0.017173549349386974 1105 8 0.0066344349780464995 1105 9 -0.17475815659587823 1105 10-11 -0.02204376979984346 1105 12-13 -0.026088514604985846 1105 14-15 0.010009132868653126 1105 16-17 -0.044510062574012466 1105 18-19 -6.859174910047727E-4 1105 20-21 0.09452564811870445 1105 22-23 -0.14062933055319604 1105 24-25 -0.012085285197173334 1105 26-27 0.0034842649154640526 1105 28-29 -0.02672037306423647 1105 30-31 -0.05680556441641471 1105 32-33 -0.10631328425546371 1105 34-35 -0.06054043935264275 1105 36-37 -0.12422214878003501 1105 38-39 0.016321893080025518 1105 40-41 0.06470861110182113 1105 42-43 0.09338194924715992 1105 44-45 0.15649650536222026 1105 46-47 0.1550349789630232 1105 48-49 0.20062158075177905 1105 50-51 0.09368433495985329 1105 52-53 0.07402969568181561 1105 54-55 0.013422365744752085 1105 56-57 -0.05457471822077764 1105 58-59 0.030266623979940732 1105 60-61 -0.01108318644929085 1105 62-63 -0.08389763404017003 1105 64-65 -0.07102211962766347 1105 66-67 -0.11857928404749885 1105 68-69 -0.1443049577399762 1105 70-71 -0.19178946711083 1105 72-73 -0.03532396698912166 1105 74-75 -0.02842794865897247 1105 76-77 -0.023649238662535765 1105 78-79 0.07641848374914062 1105 80-81 0.26257469483883256 1105 82-83 0.04421251299716644 1105 84-85 -0.2610114291886596 1105 86-87 -0.33659145184007144 1105 88-89 -0.1709925948469646 1105 90-91 -0.16881804251243437 1105 92-93 -0.1521901570406783 1105 94-95 -0.27045541110844695 1105 96-97 -0.0663814532539071 1105 98-99 -0.14890207082859064 1105 100-101 0.1694475692480708 1106 1 0.10265313615858673 1106 2 0.06581516750281224 1106 3 0.027115454699632835 1106 4 0.07069474149194832 1106 5 0.06468461478910825 1106 6 0.16353674653701944 1106 7 0.0512470187679952 1106 8 0.10464171729735483 1106 9 0.17575457234651992 1106 10-11 0.20036995814677283 1106 12-13 0.09676954063407806 1106 14-15 0.1489869250070015 1106 16-17 0.1174006109176986 1106 18-19 0.1969979686296952 1106 20-21 0.1059936949114757 1106 22-23 0.1584988318421452 1106 24-25 0.10048125052378509 1106 26-27 0.0999792472198422 1106 28-29 0.06670116438566964 1106 30-31 0.11980947978957346 1106 32-33 0.05847772225024528 1106 34-35 0.09673781742563392 1106 36-37 0.042757332614527854 1106 38-39 0.0793268040161692 1106 40-41 0.3015536700273884 1106 42-43 0.07820632517658055 1106 44-45 0.01061250851398654 1106 46-47 0.23003143605988186 1106 48-49 0.29933423146687943 1106 50-51 0.07625325151172291 1106 52-53 0.14325915676374024 1106 54-55 0.3069793838107202 1106 56-57 0.21126612134334266 1106 58-59 0.3132770335017554 1106 60-61 0.16360168412609966 1106 62-63 -0.006991788344464567 1106 64-65 0.13794388172902927 1106 66-67 0.14648920263213228 1106 68-69 0.14464486908291718 1106 70-71 0.17460878921395562 1106 72-73 0.200166758162851 1106 74-75 0.30853624628950627 1106 76-77 0.11763833736816309 1106 78-79 0.2748267282264649 1106 80-81 0.42954453953685245 1106 82-83 0.39546046519915024 1106 84-85 0.18579428934043918 1106 86-87 0.24053528205672592 1106 88-89 0.11335022968768627 1106 90-91 0.2287550215980545 1106 92-93 0.3082285290807789 1106 94-95 0.3133983983154849 1106 96-97 0.37485576631349815 1106 98-99 0.39466354468233433 1106 100-101 0.4775047923300342 1107 1 0.11990378891469788 1107 2 0.0388377497892165 1107 3 0.01209395707862626 1107 4 0.06295969804010326 1107 5 0.037770562408340425 1107 6 0.06700301661125252 1107 7 0.08831611120869809 1107 8 0.017807356141048558 1107 9 0.02546523505471754 1107 10-11 0.013298629282552099 1107 12-13 0.05217590810455164 1107 14-15 0.10163643457796212 1107 16-17 0.13671632123376298 1107 18-19 0.20504918645547576 1107 20-21 0.10919578745841818 1107 22-23 0.058240245373625044 1107 24-25 0.12113322388798053 1107 26-27 0.15872217220087492 1107 28-29 0.14689819329702658 1107 30-31 0.20732427279926213 1107 32-33 0.2734109743268647 1107 34-35 0.16527357504205753 1107 36-37 0.2591874043105378 1107 38-39 0.24333874279034973 1107 40-41 0.2961845762821014 1107 42-43 0.24421007864070532 1107 44-45 0.2471031127397012 1107 46-47 0.36361496927356995 1107 48-49 0.4007943364669657 1107 50-51 0.3050980828574339 1107 52-53 0.3308260398043785 1107 54-55 0.33658795378561734 1107 56-57 0.44245649503107387 1107 58-59 0.5459060797975823 1107 60-61 0.532549409590203 1107 62-63 0.5430575993414095 1107 64-65 0.4055327003569502 1107 66-67 0.2158701419672795 1107 68-69 0.3368959411684358 1107 70-71 0.17043590249439688 1107 72-73 0.33527487992120086 1107 74-75 0.34404850841325185 1107 76-77 0.38807952781640154 1107 78-79 0.46571386152289307 1107 80-81 0.6691473134474855 1107 82-83 0.6836032272940074 1107 84-85 0.6206701957908436 1107 86-87 0.5430769773775665 1107 88-89 0.4332870134497604 1107 90-91 0.4760895775095726 1107 92-93 0.673820873803102 1107 94-95 0.613955360954165 1107 96-97 0.5708196741653957 1107 98-99 0.820906670009645 1107 100-101 0.8995890085847371 1108 1 0.030737497050708384 1108 2 -0.016443440407705623 1108 3 -0.02855633291576254 1108 4 0.02166783405084516 1108 5 -0.08520476822641143 1108 6 0.021624701077371355 1108 7 -0.028959279100526203 1108 8 0.0036905235323345664 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#Quality Count 2 3.0 3 10.0 4 25.0 5 56.0 6 96.0 7 280.0 8 610.0 9 1213.0 10 2049.0 11 3383.0 12 5083.0 13 7254.0 14 9049.0 15 12494.0 16 16793.0 17 23319.0 18 20711.0 19 12132.0 20 10520.0 21 10820.0 22 11099.0 23 12282.0 24 14283.0 25 16964.0 26 20591.0 27 25832.0 28 31877.0 29 40370.0 30 49854.0 31 62540.0 32 79226.0 33 102775.0 34 140711.0 35 197905.0 36 302964.0 37 449520.0 38 328594.0 39 23942.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24320626881379 19.760756690149964 15.341209740739131 21.65482730029711 2 15.681706491377364 21.1837108351807 39.600909404441694 23.533673269000232 3 19.11196241621492 26.121404770663 30.220858961639003 24.545773851483084 4 13.1369832768993 17.503814914606483 37.090055431051155 32.26914637744306 5 13.65974207030565 38.27189742602754 33.17201940065858 14.896341103008234 6 30.914421395945446 36.477404335323506 17.629439598598886 14.978734670132162 7 27.970881616077147 33.96820775790105 20.611030812869494 17.449879813152315 8 27.210172589556507 33.87113397781487 19.72092005429784 19.197773378330783 9 27.470245102397094 16.363361934637407 20.304499845156325 35.861893117809174 10-11 24.911990793409043 27.182303494137685 26.77961283276077 21.1260928796925 12-13 26.25650637155485 24.69148466411101 27.71916995892975 21.33283900540439 14-15 23.381238737825615 25.33829874430267 26.59118740502406 24.689275112847657 16-17 21.79236465299306 28.99075506227077 26.91257961678435 22.304300667951818 18-19 21.77328098381276 26.916609167679262 30.055015845296175 21.255094003211802 20-21 23.046130557314733 25.833986461023233 30.039974101217737 21.079908880444297 22-23 22.74310787899155 25.984562547716937 29.932582041383743 21.33974753190776 24-25 22.27577156359002 26.193191379815257 30.17846243427675 21.352574622317977 26-27 22.08521841459486 26.369318224839034 29.951182267441645 21.594281093124458 28-29 21.796248377552015 26.357910194175943 30.191503819378124 21.654337608893922 30-31 21.981855205665028 26.084894770209505 30.29135013443077 21.6418998896947 32-33 21.877461421398277 26.334781885550452 29.987937279716142 21.79981941333513 34-35 22.183284439929746 26.29702260299183 29.890425205304666 21.629267751773753 36-37 22.564560412717643 25.972070232349093 29.694663201297562 21.768706153635705 38-39 22.256490596103614 26.15429219321012 29.87955509245622 21.709662118230042 40-41 22.20293679637269 26.158918802467873 29.851518776137 21.78662562502244 42-43 22.239990229902602 26.285225780844502 29.439916954172134 22.034867035080755 44-45 22.226016088598666 26.47206086078106 29.23058435963118 22.071338690989094 46-47 22.560486835311583 26.60038364951609 28.748044261841038 22.09108525333129 48-49 22.681418276475696 26.85939109676732 28.178321591216054 22.280869035540928 50-51 22.632847592639223 26.94391735626409 28.023003704909538 22.400231346187148 52-53 22.774527875386116 27.055296971822614 28.004614207372132 22.165560945419145 54-55 22.491377200639512 27.056173123243628 28.01587933502964 22.43657034108722 56-57 22.566017865281108 27.37981087440425 27.789807694468234 22.264363565846413 58-59 22.795103568035444 26.97043041610391 27.802980160755762 22.43148585510489 60-61 22.71533085599915 26.990014544060198 28.220019514196636 22.074635085744013 62-63 22.604522457811846 26.891124704020434 28.3075036848616 22.19684915330612 64-65 22.58727466131264 26.904911073577566 28.125419046607142 22.38239521850266 66-67 22.511061987428278 27.186368202437116 27.955340271056244 22.347229539078363 68-69 22.469350850575566 27.101844371000027 28.050724842980813 22.378079935443594 70-71 22.459819513877424 27.1275800654377 27.909818488055034 22.502781932629848 72-73 22.504378454826803 27.251356282868944 27.593840607145736 22.650424655158517 74-75 22.53235494041933 27.468414441220094 27.50321980109897 22.496010817261602 76-77 22.526448451927504 27.387297171545217 27.551892454970773 22.53436192155651 78-79 22.466090371937263 27.378095100843435 27.480562876620983 22.67525165059832 80-81 22.582647872766273 27.58588518259722 27.337573382889857 22.493893561746646 82-83 22.584988022150227 27.502330111884447 27.4420744932373 22.47060737272802 84-85 22.465495807719268 27.418321964229452 27.334329809033246 22.78185241901803 86-87 22.536902320160568 27.174170549557473 27.418916007930722 22.870011122351237 88-89 22.641020962976587 27.20324227501454 27.281837256610174 22.873899505398704 90-91 22.404076767893866 27.248816682216287 27.596118619975478 22.75098792991437 92-93 22.523060495578402 27.21148247578184 27.322877223010284 22.942579805629475 94-95 22.329815861497288 27.066923797228327 27.34432586023215 23.25893448104224 96-97 22.421096105135668 27.026679819452852 27.292601215843032 23.259622859568445 98-99 22.25779341738046 27.239220710772074 27.154887018985953 23.348098852861522 100-101 22.091173597947837 27.272018989798042 27.234528841647233 23.402278570606892 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 624.0 1 448.0 2 326.5 3 400.0 4 373.5 5 400.5 6 681.0 7 1412.5 8 2419.5 9 3057.5 10 2938.0 11 2644.5 12 2776.5 13 3051.5 14 3310.0 15 3594.5 16 3813.5 17 3963.5 18 4119.5 19 4356.5 20 4712.0 21 5193.0 22 5871.5 23 6869.5 24 8011.5 25 9632.5 26 11775.0 27 14102.5 28 16150.5 29 18485.5 30 22163.5 31 26271.0 32 30145.5 33 34271.5 34 38872.5 35 42306.5 36 46815.5 37 51803.5 38 54663.0 39 55809.5 40 57246.0 41 61676.5 42 64808.5 43 67703.0 44 73359.0 45 77914.5 46 78124.0 47 77473.0 48 80307.5 49 79305.5 50 74505.0 51 71219.5 52 68308.0 53 67676.0 54 67018.5 55 63575.5 56 58809.5 57 52914.5 58 48502.0 59 44180.0 60 37907.0 61 30890.0 62 24784.5 63 19764.0 64 14865.5 65 11210.0 66 8040.0 67 5899.5 68 4095.0 69 2368.0 70 1532.0 71 1006.5 72 662.0 73 469.5 74 303.0 75 199.0 76 134.5 77 63.0 78 33.5 79 21.0 80 10.5 81 6.5 82 4.5 83 2.0 84 0.5 85 1.0 86 1.5 87 1.5 88 1.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025693266361506213 2 0.008548139949170318 3 0.008596986463165576 4 4.884651399525896E-5 5 0.0040054141476112345 6 0.0 7 0.0 8 9.769302799051792E-5 9 1.4653954198577687E-4 10-11 0.0 12-13 2.4423256997629483E-4 14-15 0.0 16-17 2.442325699762948E-5 18-19 0.004469456030566195 20-21 0.0013921256488648801 22-23 0.0 24-25 0.005055614198509303 26-27 0.00608139099240974 28-29 0.008206214351203505 30-31 0.031090806157982327 32-33 6.10581424940737E-4 34-35 0.004322916488580418 36-37 0.01509357282453502 38-39 0.007424670127279361 40-41 0.009891419084039938 42-43 0.008132944580210615 44-45 0.0031750234096918325 46-47 0.00234463267177243 48-49 0.0019538605598103586 50-51 0.003932144376618346 52-53 0.003590218778651533 54-55 0.0019538605598103586 56-57 8.792372519146613E-4 58-59 0.001172316335886215 60-61 7.326977099288844E-5 62-63 7.326977099288844E-5 64-65 0.008377177150186911 66-67 7.571209669265138E-4 68-69 0.001318855877871992 70-71 0.004445032773568565 72-73 0.013383944834700954 74-75 0.006374470076381294 76-77 0.0042252234605899 78-79 0.011918549414843185 80-81 2.1980931297866532E-4 82-83 0.005715042137445298 84-85 6.838511959336254E-4 86-87 0.0 88-89 0.0011967395928838445 90-91 9.280837659099201E-4 92-93 2.4423256997629483E-4 94-95 9.769302799051793E-4 96-97 3.663488549644422E-4 98-99 2.1980931297866532E-4 100-101 0.0011967395928838445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2047229.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.69603157554981 #Duplication Level Percentage of deduplicated Percentage of total 1 71.09849895339659 33.20017752102002 2 14.814211067745504 13.835297355726068 3 5.533878196843478 7.7523045304514895 4 2.690764748743851 5.025921426788768 5 1.490326641063094 3.479616994448266 6 0.9195844886587572 2.576456779127709 7 0.6293078909901497 2.0570326803893075 8 0.45202670892382735 1.6886282778319124 9 0.33545277389864087 1.4097881989869032 >10 1.8048984740348364 15.37994010858317 >50 0.14968182857289128 4.80688224988368 >100 0.07749973349046653 6.254260700469456 >500 0.003032062581123381 0.915617423851635 >1k 5.227694105385135E-4 0.36904938183291586 >5k 2.0910776421540538E-4 0.7042159390869133 >10k+ 1.0455388210770269E-4 0.5448104315218293 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11159 0.5450782496730947 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8827 0.43116817903615084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5597 0.2733939388314644 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2393 0.11688970799065468 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.442325699762948E-5 0.0 18-19 0.0 0.0 0.0 4.884651399525896E-5 0.0 20-21 0.0 0.0 0.0 4.884651399525896E-5 0.0 22-23 0.0 0.0 0.0 1.4653954198577687E-4 0.0 24-25 0.0 0.0 0.0 3.419255979668127E-4 0.0 26-27 0.0 0.0 0.0 5.128883969502191E-4 0.0 28-29 0.0 0.0 0.0 0.001270009363876733 0.0 30-31 0.0 0.0 0.0 0.002515595470755836 0.0 32-33 0.0 0.0 0.0 0.003785604834632569 0.0 34-35 0.0 0.0 0.0 0.005299846768485597 0.0 36-37 0.0 0.0 0.0 0.007839865496239062 0.0 38-39 0.0 0.0 0.0 0.011478930788885854 0.0 40-41 0.0 0.0 0.0 0.016070503104440196 0.0 42-43 0.0 0.0 0.0 0.021883238269876013 0.0 44-45 0.0 0.0 0.0 0.032434085292851945 0.0 46-47 0.0 0.0 0.0 0.055172137557644996 0.0 48-49 0.0 0.0 0.0 0.07239053374097378 0.0 50-51 0.0 0.0 0.0 0.09363876732891142 0.0 52-53 0.0 0.0 0.0 0.11845279643850298 0.0 54-55 0.0 0.0 0.0 0.1466372350137674 0.0 56-57 0.0 0.0 0.0 0.17643360855087536 0.0 58-59 0.0 0.0 0.0 0.2248649271771746 0.0 60-61 0.0 0.0 0.0 0.28330978117250194 0.0 62-63 0.0 0.0 0.0 0.3494968076360778 0.0 64-65 0.0 0.0 0.0 0.40195796366698594 0.0 66-67 0.0 0.0 0.0 0.48289663735713007 0.0 68-69 0.0 0.0 0.0 0.5861337446861099 0.0 70-71 0.0 0.0 0.0 0.6574740783761855 0.0 72-73 0.0 0.0 0.0 0.7775876562905273 0.0 74-75 0.0 0.0 0.0 0.8731802841792491 0.0 76-77 0.0 0.0 0.0 0.956243781228187 0.0 78-79 0.0 0.0 0.0 1.059090116445205 0.0 80-81 0.0 0.0 0.0 1.1562214095247771 0.0 82-83 0.0 0.0 0.0 1.2974366814850709 0.0 84-85 0.0 0.0 0.0 1.4975852725806442 0.0 86-87 0.0 0.0 0.0 1.7606970202161067 0.0 88-89 0.0 0.0 0.0 2.0815697706509626 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6730 0.0 47.01811 1 GTATCAA 11915 0.0 42.826855 1 ATCAACG 15660 0.0 32.391937 3 TCAACGC 15885 0.0 31.900112 4 TATCAAC 16000 0.0 31.884056 2 CAACGCA 16120 0.0 31.405605 5 AACGCAG 16610 0.0 30.534822 6 ACGCAGA 19005 0.0 26.462599 7 CGCAGAG 19075 0.0 26.265902 8 TATCACG 115 1.2823186E-4 24.78236 2 GCAGAGT 20045 0.0 24.56841 9 AGTACTT 10400 0.0 22.946568 12-13 GTACATG 13295 0.0 22.621496 1 GAGTACT 9945 0.0 22.539911 12-13 GTATAGG 540 0.0 21.996431 1 TACATGG 13860 0.0 21.487879 2 GTACTTT 10450 0.0 21.337566 14-15 AGAGTAC 18815 0.0 20.974962 10-11 ACATGGG 13705 0.0 20.828253 3 TACTTTT 11815 0.0 19.917532 14-15 >>END_MODULE