FastQCFastQC Report
Thu 26 May 2016
SRR937129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125267
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113780.5353680266997041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82690.3890805249411015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52530.24716894394916028No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.10074028345614927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65350.048.9415321
GTATCAA116600.041.7766271
ATCAACG152300.031.9831943
TATCAAC153300.031.8675632
TCAACGC155250.031.3754624
CAACGCA157550.030.85715
AACGCAG162100.030.0181646
ACGCAGA182650.026.2759657
CGCAGAG183600.025.9335658
GTACATG140250.024.7698061
GCAGAGT190950.024.3629689
TACATGG146150.023.5416892
GAGTACT91050.023.22181312-13
ACATGGG148100.022.2049053
GTACTTT98250.021.4913314-15
GTGGTAT33050.021.281311
AGTACTT95400.021.26647812-13
ATACCGT1353.7871723E-421.119556
TGGTATC34150.020.5953352
AGAGTAC177500.020.26560610-11
Produced by FastQC (version 0.11.5)