##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937129_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2125267 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.654198272499407 33.0 31.0 34.0 30.0 34.0 2 31.757937238003507 33.0 31.0 34.0 30.0 34.0 3 31.75611064398026 33.0 31.0 34.0 30.0 34.0 4 35.342588954705455 37.0 35.0 37.0 33.0 37.0 5 35.34153873372146 37.0 35.0 37.0 33.0 37.0 6 35.44239570839805 37.0 35.0 37.0 33.0 37.0 7 35.37885075145852 37.0 35.0 37.0 33.0 37.0 8 35.33969708276654 37.0 35.0 37.0 33.0 37.0 9 36.90761913679552 39.0 37.0 39.0 33.0 39.0 10-11 36.77672899452163 39.0 37.0 39.0 32.5 39.0 12-13 36.671864288110626 39.0 37.0 39.0 32.0 39.0 14-15 37.79142479509633 40.0 37.5 41.0 32.5 41.0 16-17 37.72069838754378 40.0 37.0 41.0 32.0 41.0 18-19 37.687513615936254 40.0 37.0 41.0 32.0 41.0 20-21 37.58985718029781 40.0 37.0 41.0 32.0 41.0 22-23 37.460256758327304 40.0 37.0 41.0 32.0 41.0 24-25 37.56583008158505 40.0 37.0 41.0 32.0 41.0 26-27 37.51624713506585 40.0 37.0 41.0 32.0 41.0 28-29 37.41093166176297 40.0 37.0 41.0 32.0 41.0 30-31 37.204363734062596 39.5 36.5 41.0 31.0 41.0 32-33 36.985473119377474 39.0 36.0 41.0 31.0 41.0 34-35 36.78708110557403 39.0 36.0 41.0 30.0 41.0 36-37 36.59585854389119 39.0 35.0 41.0 30.0 41.0 38-39 36.42612198843722 39.0 35.0 41.0 30.0 41.0 40-41 36.21723458746595 39.0 35.0 41.0 30.0 41.0 42-43 35.958666134655076 39.0 35.0 40.0 29.0 41.0 44-45 35.65179739769168 39.0 35.0 40.0 27.5 41.0 46-47 35.236105392875345 38.0 35.0 40.0 26.5 41.0 48-49 34.932961129119306 38.5 34.0 40.0 24.5 41.0 50-51 34.21143602192101 38.0 33.5 39.5 23.5 40.5 52-53 34.38471260316939 38.0 34.0 39.5 24.0 40.5 54-55 34.7751830711153 38.0 34.0 40.0 24.5 41.0 56-57 34.67740994425641 38.0 34.0 40.0 24.0 41.0 58-59 34.41590586029896 38.0 34.0 40.0 23.0 41.0 60-61 34.13609254743051 38.0 34.0 40.0 23.0 41.0 62-63 33.78886558724151 37.0 33.5 40.0 22.0 41.0 64-65 33.44228725143711 37.0 33.0 40.0 21.5 41.0 66-67 33.07194790113431 36.0 33.0 39.0 20.5 41.0 68-69 32.674091302410474 36.0 32.0 39.0 19.5 41.0 70-71 32.279160453721815 35.0 32.0 38.5 18.0 40.0 72-73 31.879537488701416 35.0 31.5 37.5 17.0 40.0 74-75 31.437811578498135 35.0 31.0 37.0 13.0 39.0 76-77 31.01004720818608 35.0 31.0 36.5 9.5 39.0 78-79 30.594209104079628 35.0 30.5 36.0 7.0 38.5 80-81 30.167229341066324 34.0 30.0 36.0 6.0 37.0 82-83 29.77218815330027 34.0 30.0 35.0 2.0 37.0 84-85 29.393575724838335 34.0 29.0 35.0 2.0 36.5 86-87 29.040007679035153 34.0 29.0 35.0 2.0 36.0 88-89 28.701119200552213 34.0 29.0 35.0 2.0 36.0 90-91 28.322726509186843 34.0 29.0 35.0 2.0 35.0 92-93 27.959526732405855 34.0 27.5 35.0 2.0 35.0 94-95 27.553333534092424 34.0 27.0 35.0 2.0 35.0 96-97 27.04016107152654 33.5 26.0 35.0 2.0 35.0 98-99 26.390515168211806 33.0 25.0 35.0 2.0 35.0 100-101 23.89347973689894 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3344005120590232 1101 2 0.37401027066885106 1101 3 0.33457445838380906 1101 4 0.300431682770558 1101 5 0.3316294530950614 1101 6 0.2531043526967167 1101 7 0.16675324253051826 1101 8 0.18731648601135475 1101 9 0.2723793490530184 1101 10-11 0.4084106828404188 1101 12-13 0.5692187189818085 1101 14-15 0.6080222818203822 1101 16-17 0.5841979158967803 1101 18-19 0.5711153660376667 1101 20-21 0.5011851663125668 1101 22-23 0.6217204583080971 1101 24-25 0.6855274692248514 1101 26-27 0.6508666028867651 1101 28-29 0.5446115069399582 1101 30-31 0.5252027081256472 1101 32-33 0.6922290203429569 1101 34-35 0.6441260897667149 1101 36-37 0.6271031816170236 1101 38-39 0.5358794877120943 1101 40-41 0.7076355177497078 1101 42-43 0.7102037666667158 1101 44-45 0.7708923806986121 1101 46-47 0.9349008403939578 1101 48-49 0.8939909202950034 1101 50-51 0.9325738816745002 1101 52-53 1.0398333244180904 1101 54-55 0.8234819346524986 1101 56-57 0.7846394919644695 1101 58-59 0.7955054689401635 1101 60-61 0.9351122172628052 1101 62-63 0.9470119455470609 1101 64-65 1.0278206260385403 1101 66-67 0.9978625451527634 1101 68-69 0.9905126775622861 1101 70-71 0.9072911264344086 1101 72-73 0.744845548212151 1101 74-75 0.7102598881728603 1101 76-77 0.7842847748641439 1101 78-79 0.8595765145239689 1101 80-81 0.7544041893422992 1101 82-83 0.7195188857626214 1101 84-85 0.7047148271181207 1101 86-87 0.843482742211183 1101 88-89 0.8373645929503155 1101 90-91 0.7044159418347995 1101 92-93 0.7784766498518607 1101 94-95 0.7392143132305655 1101 96-97 0.6538645436327641 1101 98-99 0.5534589921091886 1101 100-101 0.8930710326203659 1102 1 0.16962325766453645 1102 2 0.261327024478625 1102 3 0.23716922571362886 1102 4 0.18006401841423525 1102 5 0.30497965777607305 1102 6 0.16624754092200078 1102 7 0.18958975593847782 1102 8 0.16708065298750086 1102 9 0.2153983503764394 1102 10-11 0.32809524053757855 1102 12-13 0.270403265405875 1102 14-15 0.4001380109276198 1102 16-17 0.6232142899523438 1102 18-19 0.4710924369998466 1102 20-21 0.41753958968129723 1102 22-23 0.43385815079609813 1102 24-25 0.08040431817099147 1102 26-27 0.2556995250713925 1102 28-29 0.20982086096643116 1102 30-31 0.12185913117022551 1102 32-33 0.35919399847103506 1102 34-35 0.28074933589739004 1102 36-37 0.3381349840648582 1102 38-39 0.2895507925104752 1102 40-41 0.3871874316011912 1102 42-43 0.41289241968571133 1102 44-45 0.03215696180124894 1102 46-47 0.11149963030228349 1102 48-49 0.10194091542417993 1102 50-51 0.1810154302821516 1102 52-53 0.24178422820078538 1102 54-55 0.16087783405570377 1102 56-57 0.2072379304927381 1102 58-59 0.15031031475195533 1102 60-61 0.07062995621294021 1102 62-63 0.1922095032429496 1102 64-65 0.1508872380838966 1102 66-67 0.00639999584363693 1102 68-69 0.12425416053077498 1102 70-71 0.06093854504589302 1102 72-73 -0.035069401648133436 1102 74-75 0.02234206601456279 1102 76-77 0.04289358633597473 1102 78-79 0.036289949621391315 1102 80-81 0.05857701898550616 1102 82-83 0.0988065413897452 1102 84-85 -0.02208749522331388 1102 86-87 -0.13861899550359524 1102 88-89 -0.0140871469552728 1102 90-91 -0.07367149139341578 1102 92-93 -0.14109528330424936 1102 94-95 0.04051729090006617 1102 96-97 0.03141188954345964 1102 98-99 -0.17941037916298086 1102 100-101 -0.2735857435058726 1103 1 0.2081571399071791 1103 2 0.3393770456128742 1103 3 0.28741402828124407 1103 4 0.23913369851042177 1103 5 0.22942526198215774 1103 6 0.22657605676847936 1103 7 0.19698604228282335 1103 8 0.2368341027606391 1103 9 0.28428999570342484 1103 10-11 0.39241541411701775 1103 12-13 0.40848878887491935 1103 14-15 0.39654749310140147 1103 16-17 0.45392392979624674 1103 18-19 0.4540564670860405 1103 20-21 0.5311420505137932 1103 22-23 0.5125309759574179 1103 24-25 0.2977259461957473 1103 26-27 0.48125779898531107 1103 28-29 0.5706480609577653 1103 30-31 0.5988519587526184 1103 32-33 0.5635085923677323 1103 34-35 0.37186721852001625 1103 36-37 0.6502102071669853 1103 38-39 0.6064878954165422 1103 40-41 0.6776796642378855 1103 42-43 0.4125520069294808 1103 44-45 0.6090837310707542 1103 46-47 0.6986310661025712 1103 48-49 0.7423602253753572 1103 50-51 0.6702268411341876 1103 52-53 0.8550497836207214 1103 54-55 0.9150821871301744 1103 56-57 1.00091162447319 1103 58-59 0.928280628995914 1103 60-61 0.9089503314145446 1103 62-63 0.9875534944918201 1103 64-65 0.8721492164141225 1103 66-67 0.723862118035079 1103 68-69 0.802745084828068 1103 70-71 0.788878004686488 1103 72-73 0.5132000649610511 1103 74-75 0.6599394674536931 1103 76-77 0.680662045936689 1103 78-79 0.6440353469342881 1103 80-81 0.7560642176821375 1103 82-83 0.8067748855086627 1103 84-85 0.6463391565000656 1103 86-87 0.7293784174797082 1103 88-89 0.5157536336712063 1103 90-91 0.27976603610165185 1103 92-93 0.4462968984458975 1103 94-95 0.6154943779248789 1103 96-97 0.5245823899371835 1103 98-99 0.344209977139041 1103 100-101 0.481330099285767 1104 1 0.2128858428472995 1104 2 0.28509541710705477 1104 3 0.16930848685207067 1104 4 0.22974427141396347 1104 5 0.2834568121130019 1104 6 0.08895338876831715 1104 7 0.1416142847331443 1104 8 0.23018775250925927 1104 9 0.21684137047938634 1104 10-11 0.27340810943591976 1104 12-13 0.3217884961294146 1104 14-15 0.3094817669218415 1104 16-17 0.469545188470903 1104 18-19 0.3510157267173284 1104 20-21 0.5777427410491214 1104 22-23 0.5002953645309844 1104 24-25 0.33869531605710534 1104 26-27 0.3012684933012366 1104 28-29 0.4545150435675396 1104 30-31 0.2674010807344729 1104 32-33 0.3942832903605975 1104 34-35 0.3818699737494953 1104 36-37 0.3402340737964096 1104 38-39 0.2586152041080041 1104 40-41 0.37200603721468894 1104 42-43 0.30052562964230134 1104 44-45 0.2898766295284858 1104 46-47 0.18523354915107149 1104 48-49 0.37733694264191087 1104 50-51 0.19193144141799223 1104 52-53 0.3630862810119879 1104 54-55 0.22451629413675533 1104 56-57 0.31733311495929684 1104 58-59 0.42396198243451266 1104 60-61 0.310170607548919 1104 62-63 0.4597514061759824 1104 64-65 0.41057393912050344 1104 66-67 0.4564241833292684 1104 68-69 0.4081311881797447 1104 70-71 0.32892491384500033 1104 72-73 0.41821955759247587 1104 74-75 0.3038859662520075 1104 76-77 0.3534542403013248 1104 78-79 0.41032125012831955 1104 80-81 0.5015511764841456 1104 82-83 0.4414752253456129 1104 84-85 0.46097724827864184 1104 86-87 0.42236031062807555 1104 88-89 0.5267109401467174 1104 90-91 0.43403989831625367 1104 92-93 0.4288790440651873 1104 94-95 0.5695442329572735 1104 96-97 0.5089614160898392 1104 98-99 0.2952775003890231 1104 100-101 0.5458350342986193 1105 1 0.16101342662185658 1105 2 0.15320222986395393 1105 3 0.16792251905454592 1105 4 0.3364388177638773 1105 5 0.3072106574244131 1105 6 0.26362288484174456 1105 7 0.0784548886318035 1105 8 0.25531702101535814 1105 9 0.29422414085733806 1105 10-11 0.29989504180561255 1105 12-13 0.42234515866272204 1105 14-15 0.3653953559365135 1105 16-17 0.38905139026981317 1105 18-19 0.40825062846474935 1105 20-21 0.24511315204258466 1105 22-23 0.2660738364848356 1105 24-25 0.12783951794074255 1105 26-27 0.12374788461497133 1105 28-29 0.02263567530394539 1105 30-31 -0.002758581201860011 1105 32-33 0.16313606902068756 1105 34-35 -0.10358457910476915 1105 36-37 0.15422912967262903 1105 38-39 0.09125561520692571 1105 40-41 0.10706410375260589 1105 42-43 -0.015006810828332107 1105 44-45 -0.10983476284192051 1105 46-47 -0.04131789028895838 1105 48-49 -0.029142419812899334 1105 50-51 0.0228095163952986 1105 52-53 -0.06592023795747792 1105 54-55 0.05677219721816584 1105 56-57 0.16503667620466445 1105 58-59 0.2640188566923456 1105 60-61 0.05648753502184434 1105 62-63 0.10389825438494427 1105 64-65 0.2460884019682581 1105 66-67 0.19670118079616117 1105 68-69 0.3626426040072701 1105 70-71 0.1957622119181721 1105 72-73 0.10507685711229087 1105 74-75 0.010915368489573751 1105 76-77 0.012853486528715763 1105 78-79 0.02206962112592592 1105 80-81 0.08868067475159336 1105 82-83 0.04669602470420742 1105 84-85 0.09924483293006503 1105 86-87 0.11261204729641605 1105 88-89 0.020030101780495357 1105 90-91 0.002808098162006445 1105 92-93 0.02830956151474595 1105 94-95 0.22874026433875017 1105 96-97 0.16289384072506508 1105 98-99 -0.06288399445827508 1105 100-101 0.06569246386853322 1106 1 0.18149453952241146 1106 2 0.29639435263120006 1106 3 0.2798971100917278 1106 4 0.2370131901600061 1106 5 0.48548444819430614 1106 6 0.37732374372594535 1106 7 0.33070028244214456 1106 8 0.4136934866211561 1106 9 0.37032701792156786 1106 10-11 0.38501356518715824 1106 12-13 0.4824390263086755 1106 14-15 0.46616495926126333 1106 16-17 0.44363801652988855 1106 18-19 0.30341557643492223 1106 20-21 0.4329331637073466 1106 22-23 0.36012127148235606 1106 24-25 0.40999904164908685 1106 26-27 0.32386627244492416 1106 28-29 0.40665443564434156 1106 30-31 0.3454118203385903 1106 32-33 0.27688977470813114 1106 34-35 0.40310269384631425 1106 36-37 0.3640327703685884 1106 38-39 0.2269678545631919 1106 40-41 0.302219105166742 1106 42-43 0.23444594541179953 1106 44-45 0.20994701374853975 1106 46-47 0.39938772169350756 1106 48-49 0.34052060259194405 1106 50-51 0.20908405672886232 1106 52-53 0.2650651525117098 1106 54-55 0.16305362137448043 1106 56-57 0.37316378183378873 1106 58-59 0.15339492736675453 1106 60-61 0.3026758791968618 1106 62-63 0.4799633418633533 1106 64-65 0.408254358881905 1106 66-67 0.4038654121746674 1106 68-69 0.29986609707655276 1106 70-71 0.224049356351685 1106 72-73 0.21146214309684908 1106 74-75 0.26282069266303765 1106 76-77 0.3577492180457078 1106 78-79 0.4012568830863401 1106 80-81 0.41149496027746935 1106 82-83 0.5118070597761637 1106 84-85 0.5127626422111717 1106 86-87 0.530386909290236 1106 88-89 0.5166921438176075 1106 90-91 0.664932931653361 1106 92-93 0.4289160816196116 1106 94-95 0.517877947388353 1106 96-97 0.30718017624529637 1106 98-99 0.30580241535888675 1106 100-101 0.2884333134033348 1107 1 0.20326128062715654 1107 2 0.2572768702966215 1107 3 0.24999187293940395 1107 4 0.17848062480398852 1107 5 0.35768715667896345 1107 6 0.26973329861845485 1107 7 0.08997649414574482 1107 8 0.274444588622174 1107 9 0.4426825708552897 1107 10-11 0.21942254295950647 1107 12-13 0.22389789654619108 1107 14-15 0.2633457769808416 1107 16-17 0.3628394158330792 1107 18-19 0.2772968019766111 1107 20-21 0.4490700513522867 1107 22-23 0.4642606339209294 1107 24-25 0.4693360748533877 1107 26-27 0.3389798734331393 1107 28-29 0.3756018878577976 1107 30-31 0.37922176940702457 1107 32-33 0.582363763077602 1107 34-35 0.35928082923643245 1107 36-37 0.3972706632829457 1107 38-39 0.31804676366392215 1107 40-41 0.4723818679803031 1107 42-43 0.47534839575601495 1107 44-45 0.5650626652191306 1107 46-47 0.4897387450867754 1107 48-49 0.5772934809290433 1107 50-51 0.5264380495901904 1107 52-53 0.5831771486509751 1107 54-55 0.6444413181959163 1107 56-57 0.7023876511544529 1107 58-59 0.5084786844190603 1107 60-61 0.564323560836911 1107 62-63 0.6366277471950355 1107 64-65 0.6259557965650089 1107 66-67 0.6265829800822402 1107 68-69 0.610159873986909 1107 70-71 0.5178631363040083 1107 72-73 0.6776558365664265 1107 74-75 0.6829841662157285 1107 76-77 0.6060191901705174 1107 78-79 0.5707209004966032 1107 80-81 0.5876594707985383 1107 82-83 0.6276861445716087 1107 84-85 0.5714529028107798 1107 86-87 0.5687422127545219 1107 88-89 0.6205898549533266 1107 90-91 0.6379596710807611 1107 92-93 0.5824553670140666 1107 94-95 0.6058522591441218 1107 96-97 0.5075395792528603 1107 98-99 0.5707538789839681 1107 100-101 0.3913075601975464 1108 1 -0.1776588564310444 1108 2 -0.01141622827381994 1108 3 -0.12687732583001932 1108 4 0.0851518124520041 1108 5 0.07712904563338441 1108 6 -0.09932062963637378 1108 7 -0.21309412152064766 1108 8 -0.06315325607288713 1108 9 -0.03931174932008474 1108 10-11 -0.15150902245136422 1108 12-13 -0.04614389119886653 1108 14-15 0.061935545264020675 1108 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#Quality Count 2 4355.0 3 1648.0 4 1679.0 5 1892.0 6 2048.0 7 2607.0 8 3273.0 9 3969.0 10 4983.0 11 6547.0 12 8298.0 13 10303.0 14 11420.0 15 14417.0 16 18440.0 17 24747.0 18 21052.0 19 13245.0 20 12860.0 21 13392.0 22 14597.0 23 16446.0 24 18682.0 25 21220.0 26 24990.0 27 29022.0 28 34963.0 29 41809.0 30 50888.0 31 62927.0 32 79475.0 33 103739.0 34 140444.0 35 197364.0 36 292956.0 37 412621.0 38 347227.0 39 54708.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.320312588247255 19.037450722733247 14.859896305946405 21.782340383073095 2 15.40298254612876 20.686296193465502 40.521874985294325 23.388846275111412 3 18.934415313368092 26.20121572165179 30.03045186501791 24.83391709996221 4 12.642478316752143 17.004965650836294 37.7124967702797 32.640059262131864 5 13.54211882703989 38.05303235027979 33.325345493681446 15.079503328998872 6 30.5714428279439 36.26063976516022 18.111255155714097 15.056662251181777 7 27.832616056491137 33.87854216717217 20.78002385955335 17.508817916783336 8 26.829290108520244 33.737310588585316 20.031333972315384 19.402065330579056 9 27.57322624735956 16.482447916078332 20.316071179790722 35.62825465677138 10-11 24.771485256995692 27.078905858961992 27.013815955592023 21.13579292845029 12-13 26.156406781049192 24.67851537282426 27.721072385024193 21.444005461102357 14-15 23.329289143420812 25.290995779014043 26.694218298720056 24.68549677884509 16-17 21.799579741962507 28.927871390687027 26.882772479451827 22.38977638789864 18-19 21.736933911462636 26.933697334862188 29.97409995155065 21.355268802124524 20-21 23.104887325256026 25.82994269084776 29.806947499395285 21.258222484500926 22-23 22.81001598054477 26.0175833049423 29.695908491146707 21.47649222336623 24-25 22.211206439198328 26.22027179601677 29.955790740170436 21.61273102461447 26-27 22.036917269865814 26.417405260660377 29.684785265652692 21.860892203821123 28-29 21.804134492523943 26.37022969638181 29.971106500343375 21.85452931075087 30-31 22.019045136446387 26.129728641154266 29.99592521786674 21.855301004532606 32-33 21.868550046288508 26.30182460243053 29.776721173787173 22.052904177493787 34-35 22.224559184591484 26.332303815145963 29.6209980303642 21.822138969898354 36-37 22.49016241253452 25.969584056967904 29.497281988568965 22.042971541928612 38-39 22.154327469049175 26.157731277465672 29.611251106625925 22.076690146859228 40-41 22.184093661687527 26.180981580658607 29.644383338367998 21.99054141928587 42-43 22.20451722809901 26.197551503613553 29.267547294723688 22.330383973563748 44-45 22.18571955900998 26.423147059176426 29.059855401459632 22.331277980353967 46-47 22.465624319203187 26.59192939051893 28.63364932500246 22.30879696527542 48-49 22.551732542590123 26.82989547621983 28.100141253438405 22.518230727751643 50-51 22.552638766910547 26.91082776323971 28.000598514019554 22.535934955830182 52-53 22.657652221109416 27.026515164882532 27.92137227735677 22.394460336651285 54-55 22.399672881387037 27.015964912858557 27.870275260525602 22.714086945228807 56-57 22.48371434937035 27.26345782081773 27.680042046610286 22.572785783201635 58-59 22.707651450845173 26.880631118121023 27.731639284712724 22.68007814632108 60-61 22.586885940146807 26.850555241859592 28.20769809900245 22.354860718991155 62-63 22.569733956955044 26.78242723106314 28.248557796369315 22.3992810156125 64-65 22.497385592467683 26.910576087199463 28.074890277227187 22.51714804310567 66-67 22.392118302636042 27.189780635470324 27.79344426381908 22.624656798074565 68-69 22.28210344020264 27.128858477680033 28.0366926712307 22.55234541088662 70-71 22.395387556993676 27.039317546698044 27.91069274893367 22.654602147374614 72-73 22.413079070879398 27.172758508554086 27.54021265090774 22.873949769658772 74-75 22.336292589904833 27.455387607077743 27.48047163622228 22.727848166795145 76-77 22.407689250069367 27.260962361204694 27.542218413881542 22.7891299748444 78-79 22.41249036691041 27.33176066405078 27.506268126386136 22.749480842652673 80-81 22.37665058255855 27.495822054842883 27.425208897257857 22.70231846534071 82-83 22.497186395474884 27.43536240779591 27.47294773967886 22.594503457050344 84-85 22.451503172803985 27.317198745985316 27.286914333704104 22.944383747506595 86-87 22.5025588897595 27.043994079846772 27.52447710411328 22.928969926280445 88-89 22.630803460923435 27.090770651233754 27.35484347632121 22.923582411521604 90-91 22.44142766342024 27.0962461604207 27.637134723727957 22.825191452431103 92-93 22.51365752415263 27.203943025419598 27.303728322291448 22.978671128136323 94-95 22.420405695072223 27.050134235609967 27.30389953858715 23.22556053073066 96-97 22.43980436197036 27.010496072061812 27.35485100660402 23.194848559363813 98-99 22.296436102116125 27.13972720084344 27.240191279463815 23.323645417576625 100-101 22.392257200211166 27.044042328196742 27.290959162812833 23.272741308779256 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 414.0 1 314.5 2 271.0 3 366.0 4 379.0 5 389.5 6 669.5 7 1420.5 8 2469.5 9 3124.0 10 2974.0 11 2778.5 12 2919.5 13 3054.5 14 3269.5 15 3469.5 16 3625.0 17 3798.5 18 3953.5 19 4196.5 20 4432.5 21 4781.0 22 5574.0 23 6728.0 24 7930.0 25 9554.0 26 11817.0 27 14208.5 28 16258.0 29 18741.0 30 22775.5 31 27333.5 32 31653.5 33 35827.5 34 40418.5 35 44134.0 36 48826.5 37 54010.5 38 56911.5 39 58509.0 40 60165.5 41 64393.5 42 67577.5 43 70440.5 44 75828.5 45 80458.5 46 81092.5 47 80699.5 48 83213.0 49 82132.0 50 77469.5 51 73992.0 52 71108.0 53 70579.0 54 70184.5 55 66682.0 56 61608.0 57 55503.0 58 50726.5 59 45963.0 60 39020.5 61 31914.5 62 25878.5 63 20338.0 64 15409.5 65 11886.5 66 8627.0 67 6348.0 68 4341.0 69 2547.0 70 1689.5 71 1091.0 72 715.0 73 494.5 74 351.5 75 249.0 76 161.5 77 81.5 78 52.5 79 34.0 80 23.0 81 23.0 82 19.0 83 11.0 84 8.5 85 9.0 86 7.5 87 6.5 88 6.5 89 7.0 90 7.0 91 5.0 92 2.0 93 2.5 94 3.5 95 2.5 96 1.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02611436586555948 2 0.01115154001826594 3 0.012892497742636571 4 0.022114868390653974 5 0.05669875832071923 6 0.0435239431092658 7 0.01562156660786621 8 0.04964082160029775 9 0.02912575219960598 10-11 0.024161670039576203 12-13 0.03705416778221277 14-15 0.019197587879546427 16-17 0.01797421218134004 18-19 0.017291944965032627 20-21 0.014162926352312437 22-23 0.008610682798914207 24-25 0.0015762725342274642 26-27 7.057936720421482E-5 28-29 0.002282066206269612 30-31 0.0 32-33 4.470026589600271E-4 34-35 4.7052911469476544E-5 36-37 0.0 38-39 2.3526455734738272E-5 40-41 4.7052911469476544E-5 42-43 1.646851901431679E-4 44-45 1.646851901431679E-4 46-47 0.0 48-49 4.2347620322528886E-4 50-51 1.646851901431679E-4 52-53 5.411084818989802E-4 54-55 0.0020703281046569677 56-57 3.058439245515975E-4 58-59 0.0010822169637979605 60-61 0.0031525450684549284 62-63 0.0022114868390653974 64-65 0.0011527963310021753 66-67 0.011410331031348062 68-69 0.015033405214497756 70-71 0.027267162196561653 72-73 0.01769189471252318 74-75 0.019244640791015906 76-77 0.03249003536967355 78-79 0.020209225476140173 80-81 0.04785281096445764 82-83 0.03613663600855799 84-85 0.019362273069689597 86-87 0.015151037493171447 88-89 0.013410079768800813 90-91 0.024585146242801493 92-93 0.033195829041715696 94-95 0.012798391919697618 96-97 0.01997396091879279 98-99 0.02950217549136179 100-101 0.0408184006997709 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2125267.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.25345124498064 #Duplication Level Percentage of deduplicated Percentage of total 1 72.60505874163928 35.03444662128646 2 14.094562600642687 13.602225785388791 3 5.264228398557756 7.6205156511674765 4 2.5110502814976052 4.846673693277584 5 1.4075543858217787 3.395967846545493 6 0.8693039365606331 2.516814907193895 7 0.5896889673063913 1.9918169483535357 8 0.43946451757091676 1.6964543738005735 9 0.30993618373476517 1.3459941477810766 >10 1.693035723967142 14.996610725226688 >50 0.14375359562323495 4.815008130286157 >100 0.06933248871953758 5.932619722808843 >500 0.0022481968463979787 0.7005188398817722 >1k 4.88738444869124E-4 0.3298834342939066 >5k 1.954953779476496E-4 0.637787217403804 >10k+ 9.77476889738248E-5 0.5366619553039848 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11378 0.5353680266997041 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8269 0.3890805249411015 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5253 0.24716894394916028 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2141 0.10074028345614927 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.7052911469476544E-5 0.0 5 0.0 0.0 0.0 4.7052911469476544E-5 0.0 6 0.0 0.0 0.0 4.7052911469476544E-5 0.0 7 0.0 0.0 0.0 4.7052911469476544E-5 0.0 8 0.0 0.0 0.0 4.7052911469476544E-5 0.0 9 0.0 0.0 0.0 4.7052911469476544E-5 0.0 10-11 0.0 0.0 0.0 4.7052911469476544E-5 0.0 12-13 0.0 0.0 0.0 4.7052911469476544E-5 0.0 14-15 0.0 0.0 0.0 4.7052911469476544E-5 0.0 16-17 0.0 0.0 0.0 4.7052911469476544E-5 0.0 18-19 0.0 0.0 0.0 4.7052911469476544E-5 0.0 20-21 0.0 0.0 0.0 4.7052911469476544E-5 0.0 22-23 0.0 0.0 0.0 1.8821164587790615E-4 2.3526455734738272E-5 24-25 0.0 0.0 0.0 4.470026589600271E-4 4.7052911469476544E-5 26-27 0.0 0.0 0.0 5.175820261642419E-4 4.7052911469476544E-5 28-29 0.0 0.0 0.0 9.881111408590075E-4 4.7052911469476544E-5 30-31 0.0 0.0 0.0 0.0022585397505348738 4.7052911469476544E-5 32-33 0.0 0.0 0.0 0.003999497474905506 4.7052911469476544E-5 34-35 0.0 0.0 0.0 0.005834561022215091 4.7052911469476544E-5 36-37 0.0 0.0 0.0 0.008257785962893133 4.7052911469476544E-5 38-39 0.0 0.0 0.0 0.010586905080632222 4.7052911469476544E-5 40-41 0.0 0.0 0.0 0.014821667112885111 4.7052911469476544E-5 42-43 0.0 0.0 0.0 0.01933874661395486 4.7052911469476544E-5 44-45 0.0 0.0 0.0 0.030090336884730246 4.7052911469476544E-5 46-47 0.0 0.0 0.0 0.05312273704903901 4.7052911469476544E-5 48-49 0.0 0.0 0.0 0.06933246505027368 4.7052911469476544E-5 50-51 0.0 0.0 0.0 0.08845947356261591 4.7052911469476544E-5 52-53 0.0 0.0 0.0 0.11219766739896682 4.7052911469476544E-5 54-55 0.0 0.0 0.0 0.1421233190935539 4.7052911469476544E-5 56-57 0.0 0.0 0.0 0.17110791255875146 4.7052911469476544E-5 58-59 0.0 0.0 0.0 0.21580817845475414 4.7052911469476544E-5 60-61 0.0 0.0 0.0 0.2704836615822859 4.7052911469476544E-5 62-63 0.0 0.0 0.0 0.33673416093130887 4.7052911469476544E-5 64-65 0.0 0.0 0.0 0.3877159905084867 4.7052911469476544E-5 66-67 0.0 0.0 0.0 0.4601774741714806 4.7052911469476544E-5 68-69 0.0 0.0 0.0 0.5616941306668762 4.7052911469476544E-5 70-71 0.0 0.0 0.0 0.6358024662313018 4.7052911469476544E-5 72-73 0.0 0.0 0.0 0.7493176151514139 4.7052911469476544E-5 74-75 0.0 4.7052911469476544E-5 0.0 0.8429999618871418 4.7052911469476544E-5 76-77 0.0 4.7052911469476544E-5 0.0 0.9259071918963594 4.7052911469476544E-5 78-79 0.0 4.7052911469476544E-5 0.0 1.0286707505456962 4.7052911469476544E-5 80-81 0.0 4.7052911469476544E-5 0.0 1.1341163251487931 4.7052911469476544E-5 82-83 0.0 4.7052911469476544E-5 0.0 1.2734399960099132 4.7052911469476544E-5 84-85 0.0 4.7052911469476544E-5 0.0 1.4699329543064472 4.7052911469476544E-5 86-87 0.0 4.7052911469476544E-5 0.0 1.7298767637195702 4.7052911469476544E-5 88-89 0.0 4.7052911469476544E-5 0.0 2.039273183087113 4.7052911469476544E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6535 0.0 48.941532 1 GTATCAA 11660 0.0 41.776627 1 ATCAACG 15230 0.0 31.983194 3 TATCAAC 15330 0.0 31.867563 2 TCAACGC 15525 0.0 31.375462 4 CAACGCA 15755 0.0 30.8571 5 AACGCAG 16210 0.0 30.018164 6 ACGCAGA 18265 0.0 26.275965 7 CGCAGAG 18360 0.0 25.933565 8 GTACATG 14025 0.0 24.769806 1 GCAGAGT 19095 0.0 24.362968 9 TACATGG 14615 0.0 23.541689 2 GAGTACT 9105 0.0 23.221813 12-13 ACATGGG 14810 0.0 22.204905 3 GTACTTT 9825 0.0 21.49133 14-15 GTGGTAT 3305 0.0 21.28131 1 AGTACTT 9540 0.0 21.266478 12-13 ATACCGT 135 3.7871723E-4 21.11955 6 TGGTATC 3415 0.0 20.595335 2 AGAGTAC 17750 0.0 20.265606 10-11 >>END_MODULE