FastQCFastQC Report
Thu 26 May 2016
SRR937129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125267
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111210.5232754284520487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89740.4222528275270825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57510.27060129386095955No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24230.11400920449054165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69900.046.0858731
GTATCAA119200.040.338481
ATCAACG157600.030.4968973
TATCAAC159700.030.0378062
TCAACGC160250.029.9629424
CAACGCA162650.029.4964795
AACGCAG165700.029.0108766
ACGCAGA190100.025.2122677
CGCAGAG191550.024.8974238
GTACATG140750.023.697581
GCAGAGT202550.023.2169869
TACATGG146050.022.7965032
GAGTACT99050.022.12832512-13
ACATGGG147000.021.8727133
AGTACTT102200.021.81805412-13
GTGGTAT38200.021.3934291
TGGTATC38600.021.1637632
AGAGTAC188300.020.47109410-11
GTACTTT105650.020.4092914-15
CATGGGG58250.019.7310754
Produced by FastQC (version 0.11.5)