FastQCFastQC Report
Thu 26 May 2016
SRR937128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174788
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114700.5274077289372573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82700.3802669501579004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51790.23813815415571543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64900.049.43381
GTATCAA120000.043.2124021
TATCAAC153600.033.4525642
ATCAACG153900.033.2329253
TCAACGC156450.032.752024
CAACGCA158000.032.3698045
AACGCAG164000.031.1819556
ACGCAGA185400.027.2969937
CGCAGAG187150.027.0919068
GTACATG144950.026.2979011
TACATGG148550.025.4703732
GCAGAGT194350.025.305849
ACATGGG150950.024.2152043
GAGTACT94400.023.23082712-13
CATGGGG62200.021.5503184
GTACTTT102350.021.42909414-15
AGTACTT98900.021.11677212-13
CATGGGA90300.021.0555784
AGAGTAC179450.020.6798610-11
CAGAGTA190300.020.57445710-11
Produced by FastQC (version 0.11.5)