##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937128_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2174788 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58833136839085 31.0 31.0 34.0 30.0 34.0 2 31.65464725757177 33.0 31.0 34.0 30.0 34.0 3 31.666346788744466 33.0 31.0 34.0 30.0 34.0 4 35.285441155643674 37.0 35.0 37.0 33.0 37.0 5 35.292235381103815 37.0 35.0 37.0 33.0 37.0 6 35.36315677666053 37.0 35.0 37.0 33.0 37.0 7 35.2990507580509 37.0 35.0 37.0 33.0 37.0 8 35.26042308491678 37.0 35.0 37.0 32.0 37.0 9 36.83740162259494 39.0 37.0 39.0 33.0 39.0 10-11 36.69820943466674 39.0 37.0 39.0 32.0 39.0 12-13 36.6051081760613 39.0 37.0 39.0 32.0 39.0 14-15 37.687552303948706 40.0 37.0 41.0 32.0 41.0 16-17 37.62597871608635 40.0 37.0 41.0 32.0 41.0 18-19 37.60474722133836 40.0 37.0 41.0 32.0 41.0 20-21 37.54788742626867 40.0 37.0 41.0 32.0 41.0 22-23 37.432914610527554 40.0 37.0 41.0 32.0 41.0 24-25 37.37845022135491 40.0 37.0 41.0 31.5 41.0 26-27 37.39412577225918 40.0 37.0 41.0 32.0 41.0 28-29 37.31318914763186 40.0 37.0 41.0 31.0 41.0 30-31 37.13208827711023 39.5 36.5 41.0 31.0 41.0 32-33 36.922306220192496 39.0 36.0 41.0 30.5 41.0 34-35 36.746169281787466 39.0 35.5 41.0 30.0 41.0 36-37 36.5574476684624 39.0 35.0 41.0 30.0 41.0 38-39 36.390567034579924 39.0 35.0 41.0 30.0 41.0 40-41 36.184748812298025 39.0 35.0 41.0 30.0 41.0 42-43 35.936797287827595 39.0 35.0 40.0 29.0 41.0 44-45 35.63627420235903 38.5 35.0 40.0 27.5 41.0 46-47 35.22827167521616 38.0 34.5 40.0 26.0 41.0 48-49 34.94681504588033 38.5 34.0 40.0 24.5 41.0 50-51 34.23079215077516 38.0 33.5 39.5 23.5 40.5 52-53 34.39874553289792 38.0 34.0 39.5 24.0 40.5 54-55 34.77528959144523 38.0 34.0 40.0 24.5 41.0 56-57 34.65826577119241 38.0 34.0 40.0 24.0 41.0 58-59 34.43454350493013 38.0 34.0 40.0 23.5 41.0 60-61 34.142966118996426 38.0 34.0 40.0 23.0 41.0 62-63 33.827859543091094 37.0 33.5 40.0 22.0 41.0 64-65 33.48713138935841 37.0 33.0 40.0 22.0 41.0 66-67 33.11772066978482 36.0 33.0 39.0 20.5 41.0 68-69 32.73007460957114 36.0 32.0 39.0 20.0 41.0 70-71 32.32375270601088 35.0 32.0 38.5 18.5 40.0 72-73 31.947930556909455 35.0 32.0 37.5 18.0 40.0 74-75 31.550592517523548 35.0 31.0 37.0 15.0 39.0 76-77 31.112474871113875 35.0 31.0 36.5 11.5 39.0 78-79 30.671994695574924 35.0 31.0 36.0 7.5 39.0 80-81 30.256342227380323 34.5 30.0 36.0 7.0 37.5 82-83 29.872046148866005 34.0 30.0 35.0 2.0 37.0 84-85 29.52253690934473 34.0 29.5 35.0 2.0 36.5 86-87 29.17642110403405 34.0 29.0 35.0 2.0 36.0 88-89 28.830882136557676 34.0 29.0 35.0 2.0 36.0 90-91 28.476373559169907 34.0 29.0 35.0 2.0 35.5 92-93 28.11161961533722 34.0 28.5 35.0 2.0 35.0 94-95 27.741171783180704 34.0 27.0 35.0 2.0 35.0 96-97 27.251347487663168 34.0 27.0 35.0 2.0 35.0 98-99 26.693620021813622 33.5 25.5 35.0 2.0 35.0 100-101 24.544577218561074 31.0 19.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3950598522689788 1101 2 0.38203514851945997 1101 3 0.43531404808211605 1101 4 0.3162042291976519 1101 5 0.3962473700440796 1101 6 0.37201104769869886 1101 7 0.3390058108365892 1101 8 0.41266416047319865 1101 9 0.42857308111418035 1101 10-11 0.4591287701915263 1101 12-13 0.5707560700873984 1101 14-15 0.6897279428801539 1101 16-17 0.749345085674527 1101 18-19 0.725815699693733 1101 20-21 0.6630117385567615 1101 22-23 0.6373943627698324 1101 24-25 0.7394031380942536 1101 26-27 0.7131645426640958 1101 28-29 0.6635212216310862 1101 30-31 0.6264839435630734 1101 32-33 0.5368453219125939 1101 34-35 0.6040549265894342 1101 36-37 0.6542400934307935 1101 38-39 0.7002489816219466 1101 40-41 0.7163734624441389 1101 42-43 0.7691644687799055 1101 44-45 0.7179413387058915 1101 46-47 0.754511068437381 1101 48-49 0.8278373093074691 1101 50-51 0.7351959270959583 1101 52-53 0.840932284838658 1101 54-55 0.9497858077838757 1101 56-57 0.8261401177737824 1101 58-59 0.8825116468146135 1101 60-61 0.8931350067086825 1101 62-63 0.9000852982986487 1101 64-65 0.6495315846472707 1101 66-67 0.7204702551043098 1101 68-69 0.7743318319089738 1101 70-71 0.7010385391641591 1101 72-73 0.5834002479754901 1101 74-75 0.5432161476200541 1101 76-77 0.7316848733443848 1101 78-79 0.8014199328757989 1101 80-81 0.9488624504201333 1101 82-83 0.8074376289290264 1101 84-85 0.7230016108031023 1101 86-87 0.7468053970451933 1101 88-89 0.8697496062595995 1101 90-91 0.8439731822187717 1101 92-93 0.7115304743215773 1101 94-95 0.6908218042196843 1101 96-97 0.9562210930187156 1101 98-99 0.7025165171079806 1101 100-101 1.0868845427520704 1102 1 0.07604576847583289 1102 2 -0.001561637135807814 1102 3 0.09562366242765563 1102 4 0.10381560044393012 1102 5 0.2134700861795693 1102 6 -0.024715405563647153 1102 7 -0.0034540934872850926 1102 8 -0.12293378489267326 1102 9 -0.04202470712306905 1102 10-11 0.0965015593664802 1102 12-13 0.09629940565432094 1102 14-15 -0.12708563059977251 1102 16-17 0.15680504575372112 1102 18-19 -0.07167429729548047 1102 20-21 -0.12361124297403592 1102 22-23 -0.07732889317849612 1102 24-25 -0.15952139252200226 1102 26-27 -0.06503046510616883 1102 28-29 -0.1365986016144234 1102 30-31 -0.03564947789745787 1102 32-33 -0.018473177803052465 1102 34-35 -0.08881376558343135 1102 36-37 0.016344623499641386 1102 38-39 0.0017634987859693751 1102 40-41 -0.08806263334551545 1102 42-43 -0.04716038400071909 1102 44-45 -0.1204189278248009 1102 46-47 0.04224773708757823 1102 48-49 0.03977999689622891 1102 50-51 0.05459735828426915 1102 52-53 0.29693627083698004 1102 54-55 -0.07982867967776741 1102 56-57 0.07525203694276428 1102 58-59 -0.18948266006062653 1102 60-61 -0.10357624055803427 1102 62-63 -0.16432172362737418 1102 64-65 -0.062039873901696296 1102 66-67 -0.22635924412171704 1102 68-69 -0.04147510466074067 1102 70-71 -0.07491398974497088 1102 72-73 -0.23177107797719287 1102 74-75 -0.04979122323289076 1102 76-77 -0.1822554765759854 1102 78-79 -0.28907064295497875 1102 80-81 -0.33574634493048094 1102 82-83 -0.11696231714037708 1102 84-85 -0.2278900180935608 1102 86-87 -0.1440726275540527 1102 88-89 0.03134057916961197 1102 90-91 -0.10600132239523319 1102 92-93 -0.1475473530052156 1102 94-95 -0.1173708821019126 1102 96-97 -0.3707720835441819 1102 98-99 -0.24785172141934808 1102 100-101 -0.08846978228926261 1103 1 0.21228726308094537 1103 2 0.24890342678606103 1103 3 0.24659425892632925 1103 4 0.13360313535395818 1103 5 -0.020506280125950127 1103 6 0.006842702773269593 1103 7 0.16381932390921605 1103 8 0.15120125575273846 1103 9 0.05632361356730087 1103 10-11 0.18194428645145422 1103 12-13 0.16229066442485873 1103 14-15 0.3640260573059635 1103 16-17 0.30859720246323974 1103 18-19 0.3321412921607134 1103 20-21 0.3201510022827563 1103 22-23 0.3575577852845484 1103 24-25 0.400527672233423 1103 26-27 0.516210220125835 1103 28-29 0.4725055433061556 1103 30-31 0.34030897800473525 1103 32-33 0.35810040165412005 1103 34-35 0.4793371238896995 1103 36-37 0.3951780284671429 1103 38-39 0.3795846291917897 1103 40-41 0.5220514603519746 1103 42-43 0.6411585945890437 1103 44-45 0.6356313686662389 1103 46-47 0.48764910241627035 1103 48-49 0.4671555069408271 1103 50-51 0.43005437499084564 1103 52-53 0.47117197144707745 1103 54-55 0.5128692864699644 1103 56-57 0.6561968823524964 1103 58-59 0.6865916199229147 1103 60-61 0.6480909749938348 1103 62-63 0.7046272821461486 1103 64-65 0.663488776975683 1103 66-67 0.6243310487969254 1103 68-69 0.5694893889787025 1103 70-71 0.4923471475079566 1103 72-73 0.5410657096116722 1103 74-75 0.706080732082274 1103 76-77 0.6072781198501183 1103 78-79 0.6548615706525389 1103 80-81 0.5588378326627215 1103 82-83 0.5904694955018464 1103 84-85 0.7122752614750709 1103 86-87 0.6297969945338302 1103 88-89 0.4577520049128836 1103 90-91 0.5011153929268524 1103 92-93 0.4715158953160312 1103 94-95 0.41235795000061515 1103 96-97 0.5394096439743201 1103 98-99 0.480445636675217 1103 100-101 0.6328059385644664 1104 1 0.01817366049039748 1104 2 0.008398033509358527 1104 3 -0.027904672768691796 1104 4 0.1784127996326461 1104 5 0.011687035020322867 1104 6 -0.015284887589068319 1104 7 -0.029872871180280924 1104 8 -0.05096714459917706 1104 9 0.05051262592119343 1104 10-11 0.13372085175852533 1104 12-13 -0.029006161711279788 1104 14-15 -0.13703300628221626 1104 16-17 0.16980072750001796 1104 18-19 0.11659874848589169 1104 20-21 0.001450489939955446 1104 22-23 -0.05225176809742749 1104 24-25 -0.11794217495091175 1104 26-27 -0.057452920025426124 1104 28-29 -0.05602633210508401 1104 30-31 0.19441696182859403 1104 32-33 -0.22858386062399205 1104 34-35 -0.22982516235689587 1104 36-37 -0.24417662660712125 1104 38-39 0.03483263120271829 1104 40-41 -0.07117385958503064 1104 42-43 -0.1774772280057988 1104 44-45 -0.18363286862314965 1104 46-47 -0.29140029097872144 1104 48-49 -0.29157206419941417 1104 50-51 -0.42541394181887426 1104 52-53 -0.18434957578382694 1104 54-55 -0.004959208070218324 1104 56-57 -0.028008099127752928 1104 58-59 -0.12049431024172463 1104 60-61 -0.10009216722750125 1104 62-63 -0.3323253501721126 1104 64-65 -0.18727936757448305 1104 66-67 -0.40566466319692296 1104 68-69 -0.38795954308596947 1104 70-71 -0.17185761217886864 1104 72-73 -0.16707817517403356 1104 74-75 -0.14512730275408003 1104 76-77 -0.11931343277776207 1104 78-79 -0.05230457479958872 1104 80-81 -0.026824331597484985 1104 82-83 -0.003990474482108652 1104 84-85 -0.001295169025958387 1104 86-87 0.12772507432032043 1104 88-89 0.06464904101346036 1104 90-91 0.022703285963277864 1104 92-93 -0.04277869070654816 1104 94-95 0.036822873370987 1104 96-97 -0.004952736904133559 1104 98-99 0.07885537784229513 1104 100-101 0.1893041161618534 1105 1 0.14677205706343344 1105 2 0.17206418523788258 1105 3 0.25786884901849305 1105 4 0.15642426545005605 1105 5 0.16115471576699036 1105 6 0.17210687017904291 1105 7 0.18913847876305567 1105 8 0.2209383811391632 1105 9 0.2968416781492209 1105 10-11 0.27501837117003447 1105 12-13 0.12123704393713552 1105 14-15 0.34680282042214117 1105 16-17 0.43514481893557644 1105 18-19 0.3149533366574886 1105 20-21 0.27374105015212535 1105 22-23 0.3525207887653039 1105 24-25 0.32239229062280117 1105 26-27 0.44087314130053556 1105 28-29 0.40660687761192094 1105 30-31 0.3554063185612222 1105 32-33 0.2587006486752941 1105 34-35 0.08220526835690833 1105 36-37 0.09087715091362014 1105 38-39 0.3339181613369888 1105 40-41 0.284068247078352 1105 42-43 0.29831886064670243 1105 44-45 0.5184616461684612 1105 46-47 0.5126854911686678 1105 48-49 0.5397282767197709 1105 50-51 0.4345662712543117 1105 52-53 0.612014212685196 1105 54-55 0.44419987352123513 1105 56-57 0.6015928939598112 1105 58-59 0.5331827689834086 1105 60-61 0.6064728524165375 1105 62-63 0.5843731428933765 1105 64-65 0.6933664407199203 1105 66-67 0.6629863079888807 1105 68-69 0.41532005689386864 1105 70-71 0.31898989494375485 1105 72-73 0.6145511597763402 1105 74-75 0.6832938532534705 1105 76-77 0.5318640400554848 1105 78-79 0.7383486483962187 1105 80-81 0.6455380913241733 1105 82-83 0.6614280777363035 1105 84-85 0.7645877628270981 1105 86-87 0.8325106735113827 1105 88-89 0.8153254494160471 1105 90-91 0.7174876017060932 1105 92-93 0.8248112792753126 1105 94-95 0.8585960792324485 1105 96-97 0.6005942536695379 1105 98-99 0.7631746540398403 1105 100-101 0.9292411284710056 1106 1 0.17077866760267923 1106 2 0.18065370643999756 1106 3 0.3097987717294117 1106 4 0.27567387064502924 1106 5 0.2914845836044293 1106 6 0.2676751402337132 1106 7 0.35003780361564907 1106 8 0.34527128523757966 1106 9 0.48033344257267174 1106 10-11 0.41206407745438867 1106 12-13 0.3893119333729729 1106 14-15 0.42406778483207574 1106 16-17 0.5678456604182074 1106 18-19 0.4608768642504302 1106 20-21 0.37686138972463823 1106 22-23 0.1999977073408914 1106 24-25 0.14211844839154963 1106 26-27 0.06340202623825064 1106 28-29 0.13588852270162022 1106 30-31 0.25194650293396137 1106 32-33 0.3115830870725844 1106 34-35 0.3254533923966889 1106 36-37 0.2598116355182398 1106 38-39 0.22974349005537675 1106 40-41 0.392422466093187 1106 42-43 0.13133013382391567 1106 44-45 0.21478732025752123 1106 46-47 0.44544940499801555 1106 48-49 0.43091186654910274 1106 50-51 0.6052691394116749 1106 52-53 0.5037583265133634 1106 54-55 0.5701103900982218 1106 56-57 0.5965538046451897 1106 58-59 0.6242794400817502 1106 60-61 0.6845287665609732 1106 62-63 0.6113543889178601 1106 64-65 0.37811241778130267 1106 66-67 0.3185276986135577 1106 68-69 0.5044980318374783 1106 70-71 0.4704377455807176 1106 72-73 0.5558922697103128 1106 74-75 0.6817486611400376 1106 76-77 0.680522399606442 1106 78-79 0.6437010095365778 1106 80-81 0.8598208965806613 1106 82-83 0.7121086106735213 1106 84-85 0.7469569788062103 1106 86-87 0.9965681706270431 1106 88-89 0.9640950027813062 1106 90-91 0.9598643949407943 1106 92-93 1.1179706894068637 1106 94-95 1.140147926898532 1106 96-97 1.221881251066801 1106 98-99 1.3535018103558336 1106 100-101 1.4897734333553352 1107 1 0.296653569506109 1107 2 0.4198604279022682 1107 3 0.30259487446196687 1107 4 0.3133818415403411 1107 5 0.40047141509420214 1107 6 0.3725095251471089 1107 7 0.3510519187091745 1107 8 0.35525441936160007 1107 9 0.4898977278140677 1107 10-11 0.35833380892634636 1107 12-13 0.49065467790683925 1107 14-15 0.4525846974681258 1107 16-17 0.5721960548247509 1107 18-19 0.7117905827084812 1107 20-21 0.624136358514896 1107 22-23 0.5721294793239551 1107 24-25 0.5414542466353822 1107 26-27 0.4892585655638513 1107 28-29 0.4727085643171449 1107 30-31 0.5284825619719271 1107 32-33 0.4220404423567814 1107 34-35 0.5205389936885965 1107 36-37 0.4267004362616902 1107 38-39 0.49003881645683833 1107 40-41 0.7523098321654089 1107 42-43 0.7870784648306284 1107 44-45 0.6214299258769671 1107 46-47 0.6651766182814498 1107 48-49 0.7563007292283288 1107 50-51 0.7061718839531252 1107 52-53 0.551934768881523 1107 54-55 0.6280690784714338 1107 56-57 0.7119804377053072 1107 58-59 0.6349581896281435 1107 60-61 0.7370170950371389 1107 62-63 0.8159443314987627 1107 64-65 0.747224323465943 1107 66-67 0.7564313599560251 1107 68-69 0.6946746786979858 1107 70-71 0.7632360677570773 1107 72-73 0.7195525384982346 1107 74-75 0.6484345250471542 1107 76-77 0.7738350257187498 1107 78-79 0.8206689306063275 1107 80-81 0.8648289671991911 1107 82-83 0.9666555776138637 1107 84-85 0.8373470526947173 1107 86-87 0.8326941143886124 1107 88-89 0.8011800497813475 1107 90-91 0.7214182597986714 1107 92-93 0.7904789511441663 1107 94-95 1.022573744834709 1107 96-97 1.0040952923306214 1107 98-99 1.2245937634303452 1107 100-101 1.364847011711472 1108 1 0.014093669684744015 1108 2 0.046535412354700156 1108 3 0.1051834780185743 1108 4 0.06984290345213395 1108 5 -0.06782489847390849 1108 6 -0.037734414463372445 1108 7 0.04272414831939386 1108 8 0.011956657262679471 1108 9 -0.07713553712520138 1108 10-11 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#Quality Count 2 4283.0 3 1674.0 4 1791.0 5 1924.0 6 2243.0 7 2667.0 8 3332.0 9 4081.0 10 5316.0 11 6677.0 12 8589.0 13 10529.0 14 11820.0 15 14862.0 16 18920.0 17 24646.0 18 21258.0 19 13107.0 20 12942.0 21 13804.0 22 14924.0 23 16867.0 24 19113.0 25 21754.0 26 25202.0 27 30131.0 28 35457.0 29 42704.0 30 52419.0 31 64465.0 32 81129.0 33 104909.0 34 142053.0 35 198798.0 36 294273.0 37 420066.0 38 365196.0 39 60850.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32104728129126 19.041862732922112 14.853243545337877 21.783846440448755 2 15.356476911769171 20.651448988058714 40.53229371824604 23.45978038192608 3 18.86526910305115 26.254633760451835 30.02322629308362 24.856870843413393 4 12.682105495597447 17.036030067993412 37.614572119940746 32.66729231646839 5 13.550771496622904 38.09810261307805 33.24958289705324 15.10154299324581 6 30.556701296692534 36.31899151865123 18.166677101094876 14.957630083561357 7 27.96586814969467 33.828570587296205 20.769783917801625 17.435777345207494 8 26.921437874206717 33.73886871643357 19.958397187208053 19.381296222151654 9 27.522828975370707 16.493690252965063 20.399498441256657 35.58398233040758 10-11 24.757218450132374 27.119197809127883 26.985753947464993 21.13782979327475 12-13 26.170103556479816 24.67209952244521 27.722541980164806 21.43525494091016 14-15 23.36464164532323 25.27263353539146 26.698750267152455 24.66397455213286 16-17 21.77954816729491 28.940838682801367 26.85847385870412 22.42113929119961 18-19 21.772219574095306 26.923836086874104 29.981885891699996 21.322058447330598 20-21 23.156295835534053 25.87884604413462 29.765262255168086 21.199595865163246 22-23 22.785486402299128 26.08909939265259 29.665383957270414 21.460030247777873 24-25 22.250388697356858 26.220206192393807 29.907945061347736 21.6214600489016 26-27 22.03164631465878 26.43944702868042 29.63235794519213 21.896548711468675 28-29 21.83693106766316 26.33633498993508 29.932781745881197 21.893952196520562 30-31 22.037905157324268 26.15686235738892 29.97924507435515 21.825987410931663 32-33 21.885375261422098 26.33313774576436 29.713875006667756 22.067611986145778 34-35 22.216750037017725 26.35135383701349 29.59911874825833 21.832777377710457 36-37 22.49670695724689 26.011117597436474 29.440319534934307 22.051855910382333 38-39 22.213158438063722 26.15404989444575 29.593334470649204 22.039457196841326 40-41 22.177397289565643 26.21897579959469 29.616721534162316 21.986905376677353 42-43 22.2079087181119 26.262280925215915 29.214715130357373 22.315095226314817 44-45 22.18643758778354 26.466952233567394 29.036065715487176 22.310544463161893 46-47 22.500766752331412 26.558277545491286 28.612507052512075 22.328448649665226 48-49 22.530140979777265 26.79146856233734 28.14930705634593 22.529083401539467 50-51 22.52502939427785 26.92819335434118 27.989675101839616 22.557102149541347 52-53 22.702862404769487 27.04016609512073 27.88496579346403 22.37200570664576 54-55 22.373246056155637 27.001146915560525 27.917161793271923 22.708445235011922 56-57 22.424619086082508 27.307918608685576 27.77703818002852 22.490424125203397 58-59 22.70887709249562 26.870600494050606 27.751076482197806 22.669445931255964 60-61 22.59216827456631 26.896653678856445 28.187594652489285 22.323583394087958 62-63 22.559264197575146 26.79308959717061 28.252158839844743 22.395487365409497 64-65 22.47082796772103 26.917383020455198 28.0635030993524 22.54828591247137 66-67 22.343633977477158 27.205722902025336 27.80228779024243 22.648355330255075 68-69 22.328911497968036 27.081878479573163 28.05303074215253 22.53617928030627 70-71 22.372751983922498 27.064484842684884 27.88996131535166 22.67280185804096 72-73 22.35877698495085 27.205267834869744 27.5755245245625 22.860430655616902 74-75 22.348398359418958 27.470902726568063 27.501369871833344 22.679329042179635 76-77 22.40886206851353 27.29038603449707 27.538718774152883 22.762033122836517 78-79 22.40610664412374 27.32694729425054 27.482152236524144 22.784793825101577 80-81 22.336011919963592 27.525158053176916 27.44543547394786 22.693394552911634 82-83 22.53339383161317 27.437572053474284 27.476529511424264 22.55250460348828 84-85 22.45008937534749 27.34407370396549 27.268126502331302 22.93771041835572 86-87 22.489058665789674 27.076714678981368 27.50997541505776 22.9242512401712 88-89 22.576840226383236 27.089829890546163 27.35175083582615 22.981579047244445 90-91 22.41610469653613 27.163334867495116 27.59807537449533 22.822485061473426 92-93 22.50420283651501 27.17690140832112 27.36578466710476 22.953111088059114 94-95 22.351812051493887 27.056618029666907 27.343487189415733 23.248082729423473 96-97 22.423003225449666 27.03406026097468 27.33915234161851 23.203784171957146 98-99 22.276278708835786 27.156611171178135 27.276185560869205 23.290924559116874 100-101 22.19763689415905 27.193893492278743 27.365510177129405 23.242959436432802 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 410.0 1 324.0 2 310.0 3 399.0 4 361.0 5 370.5 6 632.5 7 1355.5 8 2455.0 9 3137.5 10 2971.0 11 2740.0 12 2869.0 13 3117.0 14 3330.5 15 3527.5 16 3746.0 17 3892.0 18 4020.0 19 4230.5 20 4654.5 21 5155.0 22 5832.0 23 6889.0 24 8045.5 25 9660.5 26 12083.0 27 14714.5 28 16913.5 29 19485.0 30 23476.0 31 27901.5 32 32211.5 33 36590.0 34 41423.0 35 45201.5 36 50013.5 37 55528.5 38 58294.5 39 59757.0 40 61820.5 41 66195.5 42 69270.5 43 72301.5 44 77767.5 45 82326.0 46 83099.0 47 82654.0 48 85202.5 49 84227.5 50 79196.0 51 75382.0 52 72595.0 53 72267.0 54 71892.0 55 67925.5 56 62444.5 57 56574.0 58 51857.0 59 46923.5 60 40000.5 61 32887.0 62 26636.0 63 20845.5 64 15716.5 65 11962.5 66 8610.0 67 6367.0 68 4437.0 69 2620.0 70 1704.5 71 1067.5 72 700.0 73 497.0 74 326.5 75 229.0 76 156.5 77 73.0 78 42.5 79 31.5 80 22.5 81 21.5 82 19.5 83 16.0 84 10.5 85 6.0 86 5.0 87 4.5 88 4.0 89 5.5 90 4.5 91 3.5 92 3.5 93 2.0 94 0.5 95 0.5 96 1.0 97 0.5 98 1.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03577360184073114 2 0.029887970689556866 3 0.024278228498593884 4 0.04818860505023938 5 0.09485982081931664 6 0.11458588147442417 7 0.055959477429524174 8 0.08543361467876409 9 0.07186907413504212 10-11 0.07308758370930868 12-13 0.07032869410719574 14-15 0.06453502594275856 16-17 0.05193609675977612 18-19 0.06179912708732989 20-21 0.057752755670897576 22-23 0.034210231066200476 24-25 0.009977984060975139 26-27 0.0029428155755871375 28-29 0.0071501222188093735 30-31 0.005678714431015804 32-33 0.0066213350450710605 34-35 0.00708114997875655 36-37 0.00586264040449 38-39 0.003540574989378275 40-41 0.007127131472125099 42-43 0.0034026305092726278 44-45 0.002276083921743177 46-47 0.0014254262944250198 48-49 3.678519469483922E-4 50-51 0.002069167201584706 52-53 0.006000584884595648 54-55 0.011955188275822747 56-57 0.008391622539760198 58-59 0.0051499272572774904 60-61 0.00259795437532302 62-63 0.008391622539760198 64-65 0.002735898855428667 66-67 0.023289626391170083 68-69 0.047360938169605495 70-71 0.06051164527301052 72-73 0.0037015102161681965 74-75 0.01446117966440867 76-77 0.022415978017167652 78-79 0.02602552524659875 80-81 0.07481188971062927 82-83 0.02857749812855322 84-85 0.011541354835505805 86-87 0.03186517490440448 88-89 0.047843743849975265 90-91 0.04425718736722844 92-93 0.04407326139375424 94-95 0.012759864409772354 96-97 0.016668291346099023 98-99 0.03804968576247432 100-101 0.07499581568410346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2174788.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.11443558401973 #Duplication Level Percentage of deduplicated Percentage of total 1 72.63939457805313 34.950034712879294 2 14.08487666615182 13.553717821248485 3 5.234926240450374 7.556265641497324 4 2.5029527111272425 4.81712627957517 5 1.3858649679371482 3.3340055363980743 6 0.8770768540126497 2.5320034676775784 7 0.6054692141600304 2.0392266651966877 8 0.40826993927524075 1.5714942155320173 9 0.33581987803371444 1.4542005500537865 >10 1.7065312185576684 15.092836664464038 >50 0.14386924317515845 4.785778073164933 >100 0.07197884173396181 6.138356006781763 >500 0.002203286730064711 0.7147832563501506 >1k 4.789753761010236E-4 0.31162300723390274 >5k 1.9159015044040945E-4 0.6198813525053628 >10k+ 9.579507522020473E-5 0.5286667494413348 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11470 0.5274077289372573 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8270 0.3802669501579004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5179 0.23813815415571543 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.2990746684274512E-5 0.0 12-13 0.0 0.0 0.0 4.5981493368549024E-5 0.0 14-15 0.0 0.0 0.0 4.5981493368549024E-5 0.0 16-17 0.0 0.0 0.0 4.5981493368549024E-5 0.0 18-19 0.0 0.0 0.0 4.5981493368549024E-5 0.0 20-21 0.0 0.0 0.0 6.897224005282353E-5 0.0 22-23 0.0 0.0 0.0 2.0691672015847064E-4 0.0 24-25 0.0 0.0 0.0 4.3682418700121577E-4 0.0 26-27 0.0 0.0 0.0 6.207501604754118E-4 0.0 28-29 0.0 0.0 0.0 0.001517389281162118 0.0 30-31 0.0 0.0 0.0 0.003011787815639961 0.0 32-33 0.0 0.0 0.0 0.004897029043750471 0.0 34-35 0.0 0.0 0.0 0.0068052610185452556 0.0 36-37 0.0 0.0 0.0 0.00866751149997149 0.0 38-39 0.0 0.0 0.0 0.011219484381925963 0.0 40-41 0.0 0.0 0.0 0.015863615212149414 0.0 42-43 0.0 0.0 0.0 0.021312422176322475 0.0 44-45 0.0 0.0 0.0 0.031980128637825844 0.0 46-47 0.0 0.0 0.0 0.05352245828099107 0.0 48-49 0.0 0.0 0.0 0.06938607349314048 0.0 50-51 0.0 0.0 0.0 0.08922708788166939 0.0 52-53 0.0 0.0 0.0 0.1141030757940544 0.0 54-55 0.0 0.0 0.0 0.1420828145088165 0.0 56-57 0.0 0.0 0.0 0.1707752663707911 0.0 58-59 0.0 0.0 0.0 0.21443469432422838 0.0 60-61 0.0 0.0 0.0 0.2688767824725904 0.0 62-63 0.0 0.0 0.0 0.3342854567893514 0.0 64-65 0.0 0.0 0.0 0.38343967320033034 0.0 66-67 0.0 0.0 0.0 0.4587113778446451 0.0 68-69 0.0 0.0 0.0 0.5598936540021372 0.0 70-71 0.0 0.0 0.0 0.6290268292817507 0.0 72-73 0.0 0.0 0.0 0.7455439334776539 0.0 74-75 0.0 0.0 0.0 0.839851976376548 0.0 76-77 0.0 0.0 0.0 0.9193999599041378 0.0 78-79 0.0 0.0 0.0 1.015225392084194 0.0 80-81 0.0 0.0 0.0 1.1100852129035106 0.0 82-83 0.0 0.0 0.0 1.24605248879431 0.0 84-85 0.0 0.0 0.0 1.432714361123935 0.0 86-87 0.0 0.0 0.0 1.6894980108405968 0.0 88-89 0.0 0.0 0.0 2.005000027588896 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6490 0.0 49.4338 1 GTATCAA 12000 0.0 43.212402 1 TATCAAC 15360 0.0 33.452564 2 ATCAACG 15390 0.0 33.232925 3 TCAACGC 15645 0.0 32.75202 4 CAACGCA 15800 0.0 32.369804 5 AACGCAG 16400 0.0 31.181955 6 ACGCAGA 18540 0.0 27.296993 7 CGCAGAG 18715 0.0 27.091906 8 GTACATG 14495 0.0 26.297901 1 TACATGG 14855 0.0 25.470373 2 GCAGAGT 19435 0.0 25.30584 9 ACATGGG 15095 0.0 24.215204 3 GAGTACT 9440 0.0 23.230827 12-13 CATGGGG 6220 0.0 21.550318 4 GTACTTT 10235 0.0 21.429094 14-15 AGTACTT 9890 0.0 21.116772 12-13 CATGGGA 9030 0.0 21.055578 4 AGAGTAC 17945 0.0 20.67986 10-11 CAGAGTA 19030 0.0 20.574457 10-11 >>END_MODULE