FastQCFastQC Report
Thu 26 May 2016
SRR937128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174788
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115830.5326036376879033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88550.40716612377850164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56570.26011730798588184No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22230.1022168597582845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67800.049.4628641
GTATCAA123700.042.624321
TATCAAC163150.032.2870752
ATCAACG163250.032.179273
TCAACGC164950.031.905224
CAACGCA168100.031.2790975
AACGCAG174250.030.233126
ACGCAGA198150.026.4666657
CGCAGAG198950.026.2432658
GCAGAGT208150.024.5355729
GAGTACT103150.023.18805912-13
GTACATG139800.022.935111
AGTACTT107100.022.37720912-13
TACATGG144300.021.8896882
GTACTTT110700.021.43295914-15
AGAGTAC192550.021.02133210-11
ACATGGG145300.020.9539873
CTAAGGG7700.020.3567052
CAGAGTA203800.020.03576310-11
TAAGGTG8550.019.4435795
Produced by FastQC (version 0.11.5)