##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937128_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2174788 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0403418632069 31.0 31.0 34.0 30.0 34.0 2 32.16922936856374 33.0 31.0 34.0 30.0 34.0 3 32.289154161233185 34.0 31.0 34.0 30.0 34.0 4 35.98233482987767 37.0 35.0 37.0 35.0 37.0 5 35.810581996957865 37.0 35.0 37.0 35.0 37.0 6 35.8969747855883 37.0 35.0 37.0 35.0 37.0 7 35.845457120418175 37.0 35.0 37.0 35.0 37.0 8 35.79637739402645 37.0 35.0 37.0 35.0 37.0 9 37.464599767885424 39.0 37.0 39.0 34.0 39.0 10-11 37.3796616037977 39.0 37.0 39.0 34.0 39.0 12-13 37.26698349448314 39.0 37.0 39.0 34.0 39.0 14-15 38.427555697382914 40.0 38.0 41.0 34.0 41.0 16-17 38.42347438003152 40.0 38.0 41.0 33.5 41.0 18-19 38.556354688365026 40.0 38.0 41.0 34.0 41.0 20-21 38.56334272581971 40.0 38.0 41.0 34.0 41.0 22-23 38.46704092536835 40.0 38.0 41.0 34.0 41.0 24-25 38.37047243225547 40.0 38.0 41.0 34.0 41.0 26-27 38.16168081670489 40.0 38.0 41.0 33.5 41.0 28-29 38.00038187630243 40.0 37.0 41.0 33.0 41.0 30-31 37.97138709612155 40.0 37.0 41.0 33.0 41.0 32-33 37.83159025155555 40.0 37.0 41.0 33.0 41.0 34-35 37.70408104146243 40.0 37.0 41.0 33.0 41.0 36-37 37.54094376095509 40.0 37.0 41.0 33.0 41.0 38-39 37.371375968600155 40.0 36.0 41.0 32.0 41.0 40-41 37.038675263979755 39.5 36.0 41.0 31.5 41.0 42-43 36.81708676891725 39.0 36.0 41.0 31.0 41.0 44-45 36.549019030820475 39.0 35.0 41.0 30.5 41.0 46-47 36.1246942230691 39.0 35.0 40.5 30.0 41.0 48-49 35.725865463668185 39.0 35.0 40.0 28.5 41.0 50-51 35.42749316255194 39.0 35.0 40.0 27.5 41.0 52-53 35.157952867130035 38.0 34.5 40.0 27.0 41.0 54-55 34.746139163909305 38.0 34.0 40.0 26.0 41.0 56-57 34.54807618029895 38.0 34.0 40.0 26.0 41.0 58-59 34.21028509445519 37.5 33.5 40.0 25.0 41.0 60-61 33.82982272295047 37.0 33.0 40.0 24.0 41.0 62-63 33.4431507806738 36.5 33.0 39.0 23.0 40.5 64-65 33.10955297711777 36.0 32.0 39.0 22.5 40.0 66-67 32.77496864062152 35.5 32.0 39.0 22.0 40.0 68-69 32.323010794615385 35.0 31.0 38.0 20.5 40.0 70-71 31.885369746384477 35.0 31.0 37.5 20.0 40.0 72-73 31.488778216543405 35.0 31.0 37.0 18.5 39.0 74-75 31.00106539120135 34.0 30.5 36.5 17.5 39.0 76-77 29.55498122115811 33.0 28.5 35.0 14.0 37.0 78-79 30.246518281322132 34.0 30.0 35.0 14.0 37.0 80-81 30.11984294561125 34.0 30.0 35.0 11.0 37.0 82-83 29.78416884772217 34.0 29.5 35.0 7.5 36.5 84-85 29.353878171113692 34.0 29.0 35.0 6.0 36.0 86-87 28.9719692218276 34.0 29.0 35.0 2.0 36.0 88-89 28.600265405179723 33.5 29.0 35.0 2.0 35.0 90-91 28.147408161163295 33.0 28.0 35.0 2.0 35.0 92-93 27.658304855461772 33.0 27.0 35.0 2.0 35.0 94-95 27.09573714771279 33.0 26.0 34.0 2.0 35.0 96-97 26.32715648605749 32.0 25.0 34.0 2.0 35.0 98-99 25.455995251031368 32.0 23.0 34.0 2.0 35.0 100-101 22.354778028938913 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24195013160272794 1101 2 0.3345144689183641 1101 3 0.2714094240092422 1101 4 0.20737355314686567 1101 5 0.10351012353697797 1101 6 0.1874899071128837 1101 7 0.18210860769647752 1101 8 0.2680670291971481 1101 9 0.3156368761743593 1101 10-11 0.3684630679936802 1101 12-13 0.2916834291271826 1101 14-15 0.47547414453512005 1101 16-17 0.5418224935274765 1101 18-19 0.3356152971459636 1101 20-21 0.312795488556425 1101 22-23 0.291103045409713 1101 24-25 0.23405778399300203 1101 26-27 0.22718621075888024 1101 28-29 0.2850295945055663 1101 30-31 0.3921732420030608 1101 32-33 0.4864289953207006 1101 34-35 0.3303914913329109 1101 36-37 0.44012365286123156 1101 38-39 0.3696681974391183 1101 40-41 0.529880048995544 1101 42-43 0.6078147634939981 1101 44-45 0.47519901044712753 1101 46-47 0.34054511953412003 1101 48-49 0.32646407611503747 1101 50-51 0.3894251064975194 1101 52-53 0.5251312166607462 1101 54-55 0.3619049960392644 1101 56-57 0.3221408568366684 1101 58-59 0.48127190140802156 1101 60-61 0.5733876800974258 1101 62-63 0.5242322935216563 1101 64-65 0.5568989091525083 1101 66-67 0.43153330032382087 1101 68-69 0.13171691760211246 1101 70-71 0.17354096750710113 1101 72-73 0.28310157164116134 1101 74-75 0.27931203332387256 1101 76-77 0.42402568260691 1101 78-79 0.3962274193062463 1101 80-81 0.31643220337969424 1101 82-83 0.42951793437186936 1101 84-85 0.40607118775482576 1101 86-87 0.07446941355307857 1101 88-89 0.1547583800074861 1101 90-91 0.3372156584435366 1101 92-93 0.23638194668853174 1101 94-95 0.11737754284712665 1101 96-97 0.10704827071669243 1101 98-99 0.2657586396909686 1101 100-101 0.7935334729814194 1102 1 -0.1269081921085906 1102 2 -0.03248933156680778 1102 3 0.02132655133309669 1102 4 0.06714872743291522 1102 5 -0.23508664691229342 1102 6 -0.020962801733865888 1102 7 -0.0028169792296068863 1102 8 0.05475570062903756 1102 9 0.1367136025870721 1102 10-11 0.11029476195172805 1102 12-13 0.1848357638050615 1102 14-15 0.2224640163282956 1102 16-17 0.18543085601407938 1102 18-19 0.18014666356687314 1102 20-21 0.1917334612728112 1102 22-23 -0.06544632896493852 1102 24-25 -0.008298820933951845 1102 26-27 0.030215447833533915 1102 28-29 -0.12549741489072375 1102 30-31 -0.10111503427568636 1102 32-33 -0.05130204711112185 1102 34-35 0.0706647734862429 1102 36-37 0.09503970055939703 1102 38-39 0.18324289385141412 1102 40-41 0.30280780125540474 1102 42-43 0.3025990056221062 1102 44-45 0.2415541231590339 1102 46-47 0.04329628963548515 1102 48-49 0.23037343621633255 1102 50-51 0.14423582733267182 1102 52-53 0.05974867942886419 1102 54-55 0.3115474017088715 1102 56-57 0.30820425772855486 1102 58-59 0.009781318481103085 1102 60-61 0.04076382472874229 1102 62-63 -0.06135738991993378 1102 64-65 0.18257849044021413 1102 66-67 0.07971745101321659 1102 68-69 0.13221395091236587 1102 70-71 -0.15279916223045475 1102 72-73 -0.1531685801045377 1102 74-75 -0.10167297052324287 1102 76-77 0.08997013206370141 1102 78-79 -0.2931539298305097 1102 80-81 0.08170211487066226 1102 82-83 0.10727105590933661 1102 84-85 0.1458740977768933 1102 86-87 0.049659108348468095 1102 88-89 -0.09200755815682626 1102 90-91 -0.10359662556797034 1102 92-93 -0.10571767691238776 1102 94-95 -0.017982682120507576 1102 96-97 0.12003094703274897 1102 98-99 0.05585031156991249 1102 100-101 -0.034793066650980364 1103 1 0.10088456372307775 1103 2 0.19157956375985208 1103 3 0.18097586020289924 1103 4 0.0794971560397002 1103 5 0.037890338982286664 1103 6 0.0678911073521391 1103 7 0.04019031629204761 1103 8 0.1541003875132887 1103 9 0.09652571639050933 1103 10-11 0.10300510127442664 1103 12-13 0.2076018475370276 1103 14-15 0.16824318213442524 1103 16-17 0.18647624975083943 1103 18-19 0.07975137318798886 1103 20-21 0.05674305280918901 1103 22-23 0.07656227457806608 1103 24-25 0.06931568675323518 1103 26-27 0.017414607574011143 1103 28-29 0.04215045385314653 1103 30-31 0.16327078178637322 1103 32-33 0.05664255455636891 1103 34-35 0.12171844701676804 1103 36-37 0.05947538144684472 1103 38-39 0.10032829480740446 1103 40-41 0.14330938930124404 1103 42-43 -0.10101675240697006 1103 44-45 -0.03905934443720582 1103 46-47 -1.0471473141393517E-4 1103 48-49 -0.1447228047573006 1103 50-51 -0.22680415197714154 1103 52-53 -0.21561307313368872 1103 54-55 -0.021191647100984312 1103 56-57 -0.18865583980555556 1103 58-59 -0.27846844475400445 1103 60-61 -0.32544698812372985 1103 62-63 -0.04126409804138831 1103 64-65 -0.24410812215661082 1103 66-67 -0.0948767925764642 1103 68-69 -0.2079489098401055 1103 70-71 -0.23418683705969556 1103 72-73 -0.06752341671350592 1103 74-75 -0.33055754275561 1103 76-77 -0.17520310168883313 1103 78-79 -0.19662400070182073 1103 80-81 -0.1687208058312457 1103 82-83 0.06395545435906058 1103 84-85 -0.2130727310620948 1103 86-87 -0.34423124113325443 1103 88-89 -0.08713922548401598 1103 90-91 -0.15812725429271168 1103 92-93 -0.14370620800255196 1103 94-95 0.05659546703857998 1103 96-97 0.3010383095370024 1103 98-99 0.06546966433192125 1103 100-101 0.4760919781808468 1104 1 -0.03617268115903727 1104 2 0.07841686349887311 1104 3 -0.04335339952459094 1104 4 -0.07129649475395183 1104 5 -0.21137219789087425 1104 6 -0.015872874874816034 1104 7 -0.013528050421200533 1104 8 0.06807780894296656 1104 9 -0.0672132771109375 1104 10-11 0.04960279018696667 1104 12-13 0.12816283703141806 1104 14-15 0.10392124158985183 1104 16-17 -9.632191278541313E-4 1104 18-19 -0.03540561958893562 1104 20-21 0.033314274373431374 1104 22-23 0.00321334177407806 1104 24-25 -0.0477228535503329 1104 26-27 -0.058625418745471336 1104 28-29 -0.10076398563791145 1104 30-31 -0.06192901712092436 1104 32-33 -0.07794354195333852 1104 34-35 0.09181145239156052 1104 36-37 0.06523276901605612 1104 38-39 -0.06891487210693015 1104 40-41 0.07138178267405948 1104 42-43 0.13031748288627654 1104 44-45 0.12453733161871128 1104 46-47 0.08256508661560957 1104 48-49 0.1924623409979489 1104 50-51 0.40368985590391304 1104 52-53 0.24726610120332992 1104 54-55 0.21994955398432836 1104 56-57 0.30055575851176997 1104 58-59 -0.12189707532748173 1104 60-61 0.19431110918444006 1104 62-63 0.2892783347367214 1104 64-65 0.2325776926754557 1104 66-67 -0.05218525547412156 1104 68-69 -0.002392614973139473 1104 70-71 0.18520419213571415 1104 72-73 0.1015182878428682 1104 74-75 -0.04186013552070733 1104 76-77 0.1536222803503371 1104 78-79 0.2442717489969084 1104 80-81 0.17053256358048685 1104 82-83 0.3334529443968428 1104 84-85 0.23209069372406432 1104 86-87 0.25543717770144525 1104 88-89 0.21847732326247993 1104 90-91 0.1560301894641718 1104 92-93 0.051573658329946426 1104 94-95 0.020493042836481123 1104 96-97 0.3217704496217273 1104 98-99 0.0943601057747614 1104 100-101 0.6645794209766258 1105 1 0.014928304394118186 1105 2 0.11929702840378553 1105 3 0.052758687596082154 1105 4 0.027640737352207623 1105 5 -0.06876810738022954 1105 6 0.04012028283914759 1105 7 0.08811221722192641 1105 8 0.0069986627945866076 1105 9 0.16423660891157965 1105 10-11 0.08855544445666652 1105 12-13 0.06557343083147771 1105 14-15 0.10968922428460104 1105 16-17 -0.0926429930746906 1105 18-19 -0.052831677121716325 1105 20-21 0.059840603508796164 1105 22-23 0.12578083062705048 1105 24-25 0.03203202342310618 1105 26-27 -0.08579373181124339 1105 28-29 -0.09332206426912393 1105 30-31 0.08606971294080523 1105 32-33 0.124216184479927 1105 34-35 0.1771820649600997 1105 36-37 0.05535879116307996 1105 38-39 -0.09053058276054315 1105 40-41 0.03045017591636423 1105 42-43 0.12575032970439537 1105 44-45 0.12494782721159936 1105 46-47 0.1151685691770794 1105 48-49 0.30344086376460666 1105 50-51 0.07270206321145878 1105 52-53 0.32338289603296033 1105 54-55 0.41392872678894577 1105 56-57 0.10940718664672033 1105 58-59 0.13239585120990682 1105 60-61 0.10679318897334866 1105 62-63 0.0671908659201037 1105 64-65 -0.018594687933983778 1105 66-67 0.05549657198991298 1105 68-69 0.12566590195304173 1105 70-71 -0.049071098679632996 1105 72-73 -0.09962767036036624 1105 74-75 -0.07684527194293977 1105 76-77 0.02842943835424805 1105 78-79 0.21916526695972394 1105 80-81 0.21154886753013002 1105 82-83 0.12248237173470145 1105 84-85 -0.15056004779205878 1105 86-87 0.006061105021903046 1105 88-89 0.06761006038993855 1105 90-91 -0.018408094082278836 1105 92-93 0.10996717603295636 1105 94-95 0.009018911593244638 1105 96-97 0.12532544537907242 1105 98-99 0.25634270595777053 1105 100-101 0.45629577579654423 1106 1 -0.012876043793099257 1106 2 0.03471685069731478 1106 3 0.00531724386631538 1106 4 0.05523234363928964 1106 5 -0.016216571056830276 1106 6 0.09552320367290434 1106 7 0.07801956999998083 1106 8 0.12888905409499785 1106 9 0.2063455724191101 1106 10-11 0.17332023068856728 1106 12-13 0.21357614878341025 1106 14-15 0.25657652091378935 1106 16-17 0.11618943972271722 1106 18-19 0.1412902159489562 1106 20-21 0.14502819417194246 1106 22-23 0.15551753975098848 1106 24-25 0.14104188403697293 1106 26-27 0.2963161748341818 1106 28-29 0.3152171383828133 1106 30-31 0.1415489957916165 1106 32-33 0.059246576562713926 1106 34-35 0.03159935310641515 1106 36-37 0.16793944686897788 1106 38-39 0.17150634859027747 1106 40-41 0.3192757066591767 1106 42-43 0.5053432370930793 1106 44-45 0.33023116780603345 1106 46-47 0.4469331319496064 1106 48-49 0.110188158003794 1106 50-51 0.2241817983397567 1106 52-53 0.29318935449122563 1106 54-55 0.4017654631888661 1106 56-57 0.20749942231759633 1106 58-59 0.35792316442975647 1106 60-61 0.2755007310220279 1106 62-63 0.21096741511787087 1106 64-65 0.14714652467340983 1106 66-67 0.4071420528857388 1106 68-69 0.3778422194389819 1106 70-71 0.39806387882170924 1106 72-73 0.5461361668390303 1106 74-75 0.37832352562590543 1106 76-77 0.37731329360186905 1106 78-79 0.3428379745317862 1106 80-81 0.37150987735216034 1106 82-83 0.6329964837033906 1106 84-85 0.46294123910888274 1106 86-87 0.5466489907371646 1106 88-89 0.8312856938329638 1106 90-91 0.6577207642760889 1106 92-93 0.49473501674554043 1106 94-95 0.8440914233570176 1106 96-97 0.755382285513452 1106 98-99 0.548195655755503 1106 100-101 1.014255618485432 1107 1 0.24778662901288584 1107 2 0.3127553024664351 1107 3 0.2819853313564096 1107 4 0.19810135604939916 1107 5 0.05789615885715449 1107 6 0.12341876689091436 1107 7 0.14969148081399908 1107 8 0.12548837297234883 1107 9 0.24848844731209851 1107 10-11 0.23633137855645003 1107 12-13 0.2025842110553171 1107 14-15 0.24578253101586967 1107 16-17 0.28526306474408614 1107 18-19 0.2630346760180373 1107 20-21 0.21073198264870285 1107 22-23 0.16675219357750848 1107 24-25 0.2620066933326868 1107 26-27 0.42005377132071686 1107 28-29 0.3787885535400193 1107 30-31 0.339943275620989 1107 32-33 0.24143607115741617 1107 34-35 0.3205329027691235 1107 36-37 0.38732587119515216 1107 38-39 0.45213462570813334 1107 40-41 0.4373798994920435 1107 42-43 0.5789729060108684 1107 44-45 0.2809326732908062 1107 46-47 0.38342649799412953 1107 48-49 0.4000581422267757 1107 50-51 0.3375618955438071 1107 52-53 0.4732827077122934 1107 54-55 0.5961098271579246 1107 56-57 0.5513435293433417 1107 58-59 0.5562786514165126 1107 60-61 0.4853292249245271 1107 62-63 0.5711914916822352 1107 64-65 0.512196468798443 1107 66-67 0.5363847236468118 1107 68-69 0.3971385760797048 1107 70-71 0.34475843460192834 1107 72-73 0.46800908907446015 1107 74-75 0.38533137202370327 1107 76-77 0.1817666360557908 1107 78-79 0.4972463874758084 1107 80-81 0.5239371995780324 1107 82-83 0.6243950007734753 1107 84-85 0.5497548784826485 1107 86-87 0.8730861137992569 1107 88-89 0.8439678233836041 1107 90-91 0.7982350795937982 1107 92-93 0.7349516799881961 1107 94-95 0.9057335348182427 1107 96-97 0.8984760877450704 1107 98-99 0.99793568680116 1107 100-101 1.4181720072948067 1108 1 0.00872637258957809 1108 2 0.02194825835770331 1108 3 0.08228350044858956 1108 4 -0.03317827079501967 1108 5 -0.10678651808395045 1108 6 -0.030507208093219162 1108 7 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2316 64-65 -0.9283066875863355 2316 66-67 -0.9886974308093386 2316 68-69 -1.02687379179536 2316 70-71 -0.8101434319242422 2316 72-73 -1.1324833569458796 2316 74-75 -0.8635176964437967 2316 76-77 -0.9357227929875656 2316 78-79 -1.0946844970233904 2316 80-81 -1.2687526401895326 2316 82-83 -1.260060164718002 2316 84-85 -1.083332263888284 2316 86-87 -1.0473646252543496 2316 88-89 -1.7517232258667654 2316 90-91 -1.5167079939539079 2316 92-93 -1.4441888757585808 2316 94-95 -1.5609356956699543 2316 96-97 -1.3141919467203813 2316 98-99 -1.7654392894033712 2316 100-101 -2.526733551134363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 28.0 5 51.0 6 163.0 7 278.0 8 745.0 9 1418.0 10 2374.0 11 3917.0 12 5768.0 13 8107.0 14 10231.0 15 14110.0 16 18560.0 17 25594.0 18 21924.0 19 13745.0 20 11947.0 21 12545.0 22 13275.0 23 14711.0 24 17130.0 25 20332.0 26 25108.0 27 30961.0 28 38577.0 29 47565.0 30 58988.0 31 73324.0 32 92992.0 33 119525.0 34 160907.0 35 225290.0 36 336268.0 37 463683.0 38 272296.0 39 12339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.144491646051414 19.749388441017842 15.36612365655933 21.739996256371413 2 15.672897058742382 21.22833510214667 39.53504733491637 23.56372050419457 3 19.047960625055175 26.148803743268296 30.230496453900706 24.572739177775816 4 13.137602377794986 17.547917314239363 37.019332459071876 32.29514784889378 5 13.55306992838175 38.39027923070733 33.17435537826622 14.882295462644702 6 30.96090285581859 36.509627605081505 17.635098225666134 14.89437131343377 7 27.994609117323428 33.95244491059568 20.555935925561307 17.497010046519584 8 27.160854299361592 33.94234288583531 19.721186616810467 19.175616197992632 9 27.459174125258357 16.331637380246782 20.301501067921656 35.907687426573204 10-11 24.886379729886315 27.171200135369517 26.828798945000614 21.113621189743554 12-13 26.21043940543199 24.677391282155394 27.743556505182777 21.36861280722984 14-15 23.297750290266585 25.33878996194921 26.624929588798587 24.73853015898562 16-17 21.80023983946941 29.00032095082371 26.898828759400917 22.30061045030596 18-19 21.781773487580566 26.918920882462892 30.06076025266039 21.238545377296155 20-21 23.078346747791027 25.859562951082598 30.000740308851338 21.061349992275037 22-23 22.73019782232891 26.03185784878247 29.894776628810288 21.34316770007833 24-25 22.298388431728142 26.145911119122157 30.143150375027126 21.412550074122578 26-27 22.12061059856979 26.402530090344605 29.86535674347429 21.611502567611314 28-29 21.804807046576755 26.381555154954288 30.135808733409004 21.67782906505995 30-31 21.982234590222127 26.124408448087816 30.22742906435018 21.66592789733988 32-33 21.906648243389576 26.32113059451854 29.95392648008884 21.81829468200305 34-35 22.27989578754343 26.25092652709138 29.839161334346155 21.63001635101904 36-37 22.534334366784268 25.996820327830406 29.651397113004812 21.81744819238051 38-39 22.25028908020645 26.113904384293075 29.843332208972857 21.792474326527618 40-41 22.227576552295265 26.138929997933115 29.79062398049634 21.84286946927528 42-43 22.268354432707923 26.293091556510333 29.391186635203066 22.047367375578677 44-45 22.27848118727338 26.47767149040735 29.17920538062857 22.0646419416907 46-47 22.592041920494616 26.644169408127294 28.636022850090598 22.127765821287497 48-49 22.677983644823822 26.86028116336071 28.203907925632205 22.257827266183263 50-51 22.622896168644477 26.94435343791995 28.010658684149114 22.42209170928646 52-53 22.751585503377925 27.0513258222374 28.029216714774925 22.16787195960975 54-55 22.53210427866992 26.98398648511947 27.984106956632093 22.499802279578514 56-57 22.600576701215708 27.331347541340907 27.76079268598292 22.307283071460464 58-59 22.813537243098228 26.935208092936065 27.785406882655785 22.46584778130993 60-61 22.738393109839805 26.981724595454153 28.2003684555514 22.079513839154643 62-63 22.651229752614853 26.872884562948002 28.290471664581407 22.185414019855738 64-65 22.59140260830081 26.906536845553873 28.102791652057963 22.399268894087353 66-67 22.491824490479072 27.223665019303027 27.897753712085958 22.38675677813194 68-69 22.486419353444433 27.116507095410768 28.021492689395643 22.375580861749157 70-71 22.495621963912154 27.089473332087227 27.902108561500455 22.51279614250016 72-73 22.54569540565234 27.18367964406531 27.607492432571373 22.66313251771098 74-75 22.55319706601377 27.464264948070337 27.456884689319843 22.525653296596047 76-77 22.50683515527505 27.380096248550434 27.539836103414313 22.573232492760205 78-79 22.51774085046646 27.341164612745274 27.440967673556393 22.700126863231873 80-81 22.536678497191506 27.553366687489067 27.38336947172802 22.52658534359141 82-83 22.60790257064373 27.484681251396687 27.420468036855123 22.486948141104456 84-85 22.505717940676885 27.39314325338179 27.30556932480018 22.795569481141143 86-87 22.483988042933976 27.107488064315444 27.490216256257526 22.918307636493054 88-89 22.636064810908408 27.12575581343217 27.36771727387329 22.87046210178613 90-91 22.466809441534153 27.122695571105215 27.62307434983981 22.78742063752082 92-93 22.524057517330426 27.14418600801549 27.34974627411959 22.98201020053449 94-95 22.29925399625159 27.052544891731976 27.34080287365941 23.307398238357028 96-97 22.449435185027657 26.995727849629137 27.24791392980357 23.306923035539636 98-99 22.232693304701986 27.226755902054123 27.166059898219242 23.374490895024653 100-101 22.032169288813172 27.313501123120183 27.203212330641062 23.451117257425583 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 607.0 1 450.0 2 341.0 3 408.0 4 391.5 5 405.5 6 655.5 7 1361.0 8 2375.5 9 3045.0 10 2939.0 11 2698.5 12 2860.0 13 3169.5 14 3424.5 15 3655.5 16 3905.0 17 4152.5 18 4395.0 19 4661.5 20 5114.5 21 5561.5 22 6177.5 23 7290.0 24 8498.5 25 10124.5 26 12354.0 27 14905.5 28 17122.0 29 19758.0 30 23538.5 31 27710.5 32 32134.0 33 36394.0 34 41231.5 35 45290.5 36 50139.5 37 55182.5 38 58096.0 39 59517.5 40 60956.5 41 65099.5 42 68303.5 43 71762.5 44 77917.0 45 82549.0 46 83195.5 47 82710.0 48 85300.5 49 84427.5 50 79347.0 51 75745.0 52 73061.5 53 72215.5 54 71310.0 55 67643.5 56 62063.5 57 55778.0 58 51314.0 59 47252.5 60 40603.5 61 33047.5 62 26556.5 63 20944.0 64 15775.5 65 11832.5 66 8479.0 67 6320.5 68 4424.0 69 2487.0 70 1602.0 71 1038.0 72 662.5 73 474.5 74 323.0 75 216.0 76 140.5 77 78.5 78 41.5 79 17.0 80 10.0 81 6.0 82 4.0 83 2.5 84 2.5 85 2.0 86 2.0 87 2.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019634097668370435 2 0.0033106675225355302 3 1.839259734741961E-4 4 0.0 5 0.0028968340822185887 6 0.0 7 2.2990746684274516E-4 8 0.0 9 1.3794448010564706E-4 10-11 0.0 12-13 0.006138529364701295 14-15 0.0028968340822185887 16-17 0.0 18-19 5.057964270540392E-4 20-21 9.196298673709806E-4 22-23 0.0015403800278463923 24-25 5.517779204225882E-4 26-27 1.839259734741961E-4 28-29 6.897224005282353E-5 30-31 0.0 32-33 1.3794448010564706E-4 34-35 0.0 36-37 0.0016323430145834905 38-39 0.010046956301027962 40-41 7.586946405810589E-4 42-43 0.0 44-45 0.001080565094160902 46-47 2.0691672015847064E-4 48-49 1.1495373342137258E-4 50-51 0.0016323430145834905 52-53 2.528982135270196E-4 54-55 0.0 56-57 2.2990746684274516E-4 58-59 1.1495373342137258E-4 60-61 0.0015863615212149413 62-63 4.5981493368549024E-5 64-65 2.2990746684274512E-5 66-67 0.0 68-69 9.196298673709805E-5 70-71 5.287871737383137E-4 72-73 3.2187045357984315E-4 74-75 0.0031037508023770595 76-77 9.196298673709805E-5 78-79 2.2990746684274516E-4 80-81 0.0021381394416375298 82-83 9.196298673709805E-5 84-85 0.0024830006419016473 86-87 3.2187045357984315E-4 88-89 0.002827861842165765 90-91 0.0016323430145834905 92-93 0.0 94-95 0.0 96-97 2.2990746684274516E-4 98-99 7.127131472125099E-4 100-101 0.0019772042148476082 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2174788.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.23027581026671 #Duplication Level Percentage of deduplicated Percentage of total 1 70.70894228271312 32.68893903982058 2 15.00935858854923 13.877735745676528 3 5.62391218861276 7.799850348368658 4 2.7105091618918875 5.012303445620673 5 1.5050130174092489 3.478858344643566 6 0.9495214732682276 2.633798375817657 7 0.6140232999433283 1.9870526557217132 8 0.45573489397805494 1.6854999875974508 9 0.33397274041820135 1.3895686712379651 >10 1.8536605771954584 15.683423520630448 >50 0.15321838128064233 4.918846932795284 >100 0.07834274286646453 6.351984689470015 >500 0.00299261989998372 0.9336295100189429 >1k 4.98769983330618E-4 0.3550974179869516 >5k 1.9950799333224719E-4 0.6692433415359275 >10k+ 9.975399666612359E-5 0.5341679730575144 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11583 0.5326036376879033 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8855 0.40716612377850164 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5657 0.26011730798588184 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2223 0.1022168597582845 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.2990746684274512E-5 0.0 12-13 0.0 0.0 0.0 4.5981493368549024E-5 0.0 14-15 0.0 0.0 0.0 4.5981493368549024E-5 0.0 16-17 0.0 0.0 0.0 4.5981493368549024E-5 0.0 18-19 0.0 0.0 0.0 4.5981493368549024E-5 0.0 20-21 0.0 0.0 0.0 9.196298673709805E-5 0.0 22-23 0.0 0.0 0.0 2.758889602112941E-4 0.0 24-25 0.0 0.0 0.0 5.287871737383138E-4 0.0 26-27 0.0 0.0 0.0 7.127131472125099E-4 0.0 28-29 0.0 0.0 0.0 0.0016323430145834903 0.0 30-31 0.0 0.0 0.0 0.003172723042429883 0.0 32-33 0.0 0.0 0.0 0.0051269365105932165 0.0 34-35 0.0 0.0 0.0 0.007173112965493648 0.0 36-37 0.0 0.0 0.0 0.008989381953551335 0.0 38-39 0.0 0.0 0.0 0.011656308568927179 0.0 40-41 0.0 0.0 0.0 0.016277448652466355 0.0 42-43 0.0 0.0 0.0 0.021634292629902317 0.0 44-45 0.0 0.0 0.0 0.032256017598037146 0.0 46-47 0.0 0.0 0.0 0.05379834724120236 0.0 48-49 0.0 0.0 0.0 0.06966196245335177 0.0 50-51 0.0 0.0 0.0 0.08954895833524923 0.0 52-53 0.0 0.0 0.0 0.11428700176752861 0.0 54-55 0.0 0.0 0.0 0.14238169421571206 0.0 56-57 0.0 0.0 0.0 0.17139601653126652 0.0 58-59 0.0 0.0 0.0 0.21517039821812517 0.0 60-61 0.0 0.0 0.0 0.26981940308664565 0.0 62-63 0.0 0.0 0.0 0.33522807740340665 0.0 64-65 0.0 0.0 0.0 0.3844512660544384 0.0 66-67 0.0 0.0 0.0 0.4593551187518048 0.0 68-69 0.0 0.0 0.0 0.5602385152024014 0.0 70-71 0.0 0.0 0.0 0.6294176719753833 0.0 72-73 0.0 0.0 0.0 0.746463563345025 0.0 74-75 0.0 0.0 0.0 0.8411624489375515 0.0 76-77 0.0 0.0 0.0 0.9206184694784043 0.0 78-79 0.0 0.0 0.0 1.0167197906186718 0.0 80-81 0.0 0.0 0.0 1.111970454131621 0.0 82-83 0.0 0.0 0.0 1.2486044616762646 0.0 84-85 0.0 0.0 0.0 1.4349444635523094 0.0 86-87 0.0 0.0 0.0 1.691268298335286 0.0 88-89 0.0 0.0 0.0 2.0058736759628983 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6780 0.0 49.462864 1 GTATCAA 12370 0.0 42.62432 1 TATCAAC 16315 0.0 32.287075 2 ATCAACG 16325 0.0 32.17927 3 TCAACGC 16495 0.0 31.90522 4 CAACGCA 16810 0.0 31.279097 5 AACGCAG 17425 0.0 30.23312 6 ACGCAGA 19815 0.0 26.466665 7 CGCAGAG 19895 0.0 26.243265 8 GCAGAGT 20815 0.0 24.535572 9 GAGTACT 10315 0.0 23.188059 12-13 GTACATG 13980 0.0 22.93511 1 AGTACTT 10710 0.0 22.377209 12-13 TACATGG 14430 0.0 21.889688 2 GTACTTT 11070 0.0 21.432959 14-15 AGAGTAC 19255 0.0 21.021332 10-11 ACATGGG 14530 0.0 20.953987 3 CTAAGGG 770 0.0 20.356705 2 CAGAGTA 20380 0.0 20.035763 10-11 TAAGGTG 855 0.0 19.443579 5 >>END_MODULE