FastQCFastQC Report
Thu 26 May 2016
SRR937127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846024
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117740.6378031921578484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78930.42756757225258174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55350.29983358829571016No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18950.10265305326474629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA116400.039.6106721
GGTATCA66650.038.51121
ATCAACG146400.031.3006233
TCAACGC148600.030.9012014
TATCAAC148850.030.8476282
CAACGCA149300.030.6901425
AACGCAG153700.029.8726066
ACGCAGA169800.026.6482777
CGCAGAG169700.026.609418
GTACATG109250.025.0172671
GCAGAGT176800.024.5998429
GAGTACT84350.024.08323712-13
TACATGG111650.023.9686242
AGTACTT89850.023.3494412-13
AGAGTAC147250.022.52892910-11
ACATGGG113150.022.4338473
GTACTTT92150.021.94129214-15
TACTTTT102800.020.4077714-15
CATGGGA73000.019.7955594
CAGAGTA173500.019.38060610-11
Produced by FastQC (version 0.11.5)