##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937127_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1846024 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.449087877514053 31.0 31.0 34.0 30.0 34.0 2 31.523000242683736 33.0 31.0 34.0 28.0 34.0 3 31.523085290332087 33.0 31.0 34.0 28.0 34.0 4 35.123683115712474 37.0 35.0 37.0 32.0 37.0 5 35.15220278826278 37.0 35.0 37.0 32.0 37.0 6 35.202042877015685 37.0 35.0 37.0 32.0 37.0 7 35.128594211126185 37.0 35.0 37.0 32.0 37.0 8 35.099406074893935 37.0 35.0 37.0 32.0 37.0 9 36.65497523325807 39.0 37.0 39.0 32.0 39.0 10-11 36.508412404172425 39.0 37.0 39.0 32.0 39.0 12-13 36.37664732419513 39.0 37.0 39.0 32.0 39.0 14-15 37.4217485796501 40.0 37.0 41.0 32.0 41.0 16-17 37.35696448150186 40.0 37.0 41.0 32.0 41.0 18-19 37.33575619818594 40.0 37.0 41.0 32.0 41.0 20-21 37.2840288100263 40.0 37.0 41.0 31.0 41.0 22-23 37.1304330279563 39.5 36.5 41.0 31.0 41.0 24-25 36.95044376454477 39.0 36.0 41.0 30.5 41.0 26-27 36.897813083686884 39.0 36.0 41.0 30.5 41.0 28-29 36.95707314747804 39.0 36.0 41.0 30.5 41.0 30-31 36.79656900451998 39.0 36.0 41.0 30.0 41.0 32-33 36.57902091197081 39.0 35.5 41.0 30.0 41.0 34-35 36.37115037507638 39.0 35.0 41.0 30.0 41.0 36-37 36.192117762282614 39.0 35.0 41.0 30.0 41.0 38-39 36.0130131027549 39.0 35.0 40.5 29.0 41.0 40-41 35.78191047353664 38.5 35.0 40.0 28.0 41.0 42-43 35.53840551368779 38.0 35.0 40.0 27.0 41.0 44-45 35.22956554194312 38.0 34.5 40.0 26.0 41.0 46-47 34.81434802581114 38.0 34.0 40.0 24.0 41.0 48-49 34.41915706404684 38.0 33.5 40.0 23.0 41.0 50-51 33.75198670223139 37.5 33.0 39.5 22.5 40.5 52-53 34.04500374859698 38.0 33.5 40.0 23.0 40.5 54-55 34.34155514771206 38.0 34.0 40.0 23.0 41.0 56-57 34.22091830875438 38.0 34.0 40.0 22.0 41.0 58-59 33.970481965564915 38.0 33.5 40.0 21.5 41.0 60-61 33.675713858541386 38.0 33.0 40.0 20.0 41.0 62-63 33.36146415214537 37.0 33.0 40.0 19.0 41.0 64-65 33.0520228881098 37.0 32.5 40.0 18.0 41.0 66-67 32.67795868309405 36.0 32.0 39.0 16.5 41.0 68-69 32.23395903845237 36.0 31.5 39.0 13.0 41.0 70-71 31.875464240984947 35.0 31.0 38.5 11.0 40.0 72-73 31.49942768891412 35.0 31.0 38.0 8.0 40.0 74-75 31.07342726855122 35.0 31.0 37.0 7.0 39.0 76-77 30.63636361174069 35.0 30.5 36.5 4.5 39.0 78-79 30.214483668684696 35.0 30.0 36.0 2.0 39.0 80-81 29.786478398980726 34.0 29.5 36.0 2.0 37.5 82-83 29.367059420679254 34.0 29.0 35.0 2.0 37.0 84-85 29.011861709273553 34.0 29.0 35.0 2.0 36.5 86-87 28.665888959190127 34.0 29.0 35.0 2.0 36.0 88-89 28.286496275238026 34.0 28.5 35.0 2.0 36.0 90-91 27.939770826381455 34.0 27.0 35.0 2.0 35.5 92-93 27.57074203802334 34.0 27.0 35.0 2.0 35.0 94-95 27.152558959146795 34.0 26.0 35.0 2.0 35.0 96-97 26.682861923788643 33.0 25.0 35.0 2.0 35.0 98-99 26.044842862281314 33.0 24.5 35.0 2.0 35.0 100-101 23.890080789848888 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4126862524813504 1101 2 0.3664310273916307 1101 3 0.44920391958190464 1101 4 0.327073992052604 1101 5 0.36784316376538584 1101 6 0.4861654635536823 1101 7 0.33318263947067095 1101 8 0.26422971038633136 1101 9 0.31778560545041046 1101 10-11 0.2661052857960158 1101 12-13 0.3005751954544138 1101 14-15 0.658659634538779 1101 16-17 0.6832337137528839 1101 18-19 0.6260184456697147 1101 20-21 0.616248267597264 1101 22-23 0.6044105884786504 1101 24-25 0.6855358884311613 1101 26-27 0.6288981724201506 1101 28-29 0.658979739142346 1101 30-31 0.6416023663265662 1101 32-33 0.7177893984114334 1101 34-35 0.7409212831454326 1101 36-37 0.6735511542480666 1101 38-39 0.6648685441182636 1101 40-41 0.7381652966312515 1101 42-43 0.7504036928393489 1101 44-45 0.8009230696453145 1101 46-47 0.9610656741093351 1101 48-49 1.0287586719012083 1101 50-51 0.9083416662504504 1101 52-53 1.0146596620622077 1101 54-55 0.9760176602000072 1101 56-57 0.9441312927505905 1101 58-59 0.9764208437057036 1101 60-61 1.0191394977786885 1101 62-63 1.1146325972455031 1101 64-65 1.105066863587659 1101 66-67 0.9742871578709256 1101 68-69 0.8521992203098705 1101 70-71 0.8981497874170756 1101 72-73 0.9407597379446315 1101 74-75 0.8983170921427615 1101 76-77 0.7981911130319084 1101 78-79 0.9187396152990921 1101 80-81 0.912585587225518 1101 82-83 0.9771485782020655 1101 84-85 1.1463470287933646 1101 86-87 1.2181317467229071 1101 88-89 1.302553055364065 1101 90-91 1.053428200830453 1101 92-93 1.0444458892208495 1101 94-95 1.226326099602307 1101 96-97 1.409191023103972 1101 98-99 1.3699355588766338 1101 100-101 1.6897683732731572 1102 1 0.3615887128884445 1102 2 0.3749155898971104 1102 3 0.378975502937152 1102 4 0.29240634813348976 1102 5 0.26054619505293886 1102 6 0.4265801383271608 1102 7 0.3798184027847711 1102 8 0.398156808384897 1102 9 0.4336844585324897 1102 10-11 0.6376223735321389 1102 12-13 0.49626935411482975 1102 14-15 0.6789914491341307 1102 16-17 0.5861252142350679 1102 18-19 0.5728113687117258 1102 20-21 0.4904149278176675 1102 22-23 0.6350246652974008 1102 24-25 0.7684887314880271 1102 26-27 0.5789348516183352 1102 28-29 0.8319572906995063 1102 30-31 0.7732818159194181 1102 32-33 0.7370332461044526 1102 34-35 0.47902503950788855 1102 36-37 0.5926606297290888 1102 38-39 0.5746029067897993 1102 40-41 0.6607101432361446 1102 42-43 0.7117329237751946 1102 44-45 0.8911393931132991 1102 46-47 0.6686702581711046 1102 48-49 0.8004372705882332 1102 50-51 0.5385674850539175 1102 52-53 0.7375620180399522 1102 54-55 0.984956361459858 1102 56-57 0.9077731181240836 1102 58-59 0.7007362190583066 1102 60-61 0.8344829414203545 1102 62-63 0.8877361408815005 1102 64-65 1.0073105153144866 1102 66-67 0.831085186347913 1102 68-69 0.7976919334617847 1102 70-71 0.8559287427110007 1102 72-73 0.8565875855138785 1102 74-75 0.8519390179231436 1102 76-77 0.8965940208091894 1102 78-79 0.9308163541122632 1102 80-81 0.9430389911066825 1102 82-83 1.0237977174979243 1102 84-85 1.0382376706342065 1102 86-87 1.1056623019776382 1102 88-89 1.0285122260660913 1102 90-91 0.8140543708259678 1102 92-93 1.0643208254029126 1102 94-95 1.2606801331537802 1102 96-97 0.9501010604406908 1102 98-99 1.1471197438893448 1102 100-101 1.5798298898967644 1103 1 0.4108053187691283 1103 2 0.48612533352498843 1103 3 0.38759600530203997 1103 4 0.45247009309488817 1103 5 0.29298568522788315 1103 6 0.18525227741553607 1103 7 0.1934944387245423 1103 8 0.28825703105774636 1103 9 0.3712130499775341 1103 10-11 0.4870616216647292 1103 12-13 0.21262141161258796 1103 14-15 0.44634666664637734 1103 16-17 0.6216300133139043 1103 18-19 0.6720569982559468 1103 20-21 0.5276138704199695 1103 22-23 0.4675804643232979 1103 24-25 0.7845606807980516 1103 26-27 0.7216148449966013 1103 28-29 0.7689278995354911 1103 30-31 0.7540134386772763 1103 32-33 0.6830718755755427 1103 34-35 0.6753804507152097 1103 36-37 0.6464480418739811 1103 38-39 0.5690651550665109 1103 40-41 0.7933605254396952 1103 42-43 0.9516252072750362 1103 44-45 0.9125183736161873 1103 46-47 0.8272225388619034 1103 48-49 0.6187564807039507 1103 50-51 0.6001446220569306 1103 52-53 0.7129725338166182 1103 54-55 0.6589305042367002 1103 56-57 0.7571828377007179 1103 58-59 0.6157599199318966 1103 60-61 0.5389486076656667 1103 62-63 0.6622282420692756 1103 64-65 0.6043203894800016 1103 66-67 0.6043153625125228 1103 68-69 0.8279427915493756 1103 70-71 0.7281934115949085 1103 72-73 0.736349906648087 1103 74-75 0.7658400216798498 1103 76-77 0.5571998366189312 1103 78-79 0.9209180149634868 1103 80-81 0.861616216542366 1103 82-83 0.9216693053049916 1103 84-85 1.1094255111114641 1103 86-87 1.165363534190508 1103 88-89 1.1551973809747338 1103 90-91 1.1460064409307371 1103 92-93 1.0250236723535018 1103 94-95 1.0745922445434495 1103 96-97 1.0313691543310917 1103 98-99 1.2560354400151077 1103 100-101 1.6276096943840663 1104 1 0.3523198706252266 1104 2 0.5070086546704431 1104 3 0.4044865317014441 1104 4 0.3636949512210137 1104 5 0.35963107178601916 1104 6 0.3905144024047331 1104 7 0.38845245480319335 1104 8 0.394222918245525 1104 9 0.4524742497784757 1104 10-11 0.4146562957578226 1104 12-13 0.4915131015063068 1104 14-15 0.4549569334323422 1104 16-17 0.6400359443313022 1104 18-19 0.6869680657035744 1104 20-21 0.5344288105747808 1104 22-23 0.7154313291173153 1104 24-25 0.522771831652804 1104 26-27 0.6818768706916032 1104 28-29 0.627361831515465 1104 30-31 0.6452186891205969 1104 32-33 0.43388652602494204 1104 34-35 0.5792166526772675 1104 36-37 0.6637640730967505 1104 38-39 0.7691771238717777 1104 40-41 0.7474368149208175 1104 42-43 0.7277115441603783 1104 44-45 0.7952038902146725 1104 46-47 0.602994346787213 1104 48-49 0.3537195432639635 1104 50-51 0.38325135046511605 1104 52-53 0.5405670034804899 1104 54-55 0.5001322209748977 1104 56-57 0.6194571186355375 1104 58-59 0.6449040799147028 1104 60-61 0.7728874798238152 1104 62-63 0.703261421516082 1104 64-65 0.5449576479322715 1104 66-67 0.6913969543035776 1104 68-69 0.7433133282753772 1104 70-71 0.5890089440103736 1104 72-73 0.5288717688931683 1104 74-75 0.32718295228372085 1104 76-77 0.44962509145456053 1104 78-79 0.4343073988955801 1104 80-81 0.41639755320575134 1104 82-83 0.3871908568176501 1104 84-85 0.49020530929893624 1104 86-87 0.5412850719187965 1104 88-89 0.590034106275592 1104 90-91 0.58747134569008 1104 92-93 0.45673744924762616 1104 94-95 0.5070802241051986 1104 96-97 0.3933414641009385 1104 98-99 0.3609039803437355 1104 100-101 0.6744454512744973 1105 1 0.3925473703629727 1105 2 0.40104431210728464 1105 3 0.3919211392096642 1105 4 0.3223984809332592 1105 5 0.3835161497553088 1105 6 0.3572400572418388 1105 7 0.37663448651997555 1105 8 0.3730253747683534 1105 9 0.4116583886500109 1105 10-11 0.5220744382689801 1105 12-13 0.37464240874498245 1105 14-15 0.47765160579714916 1105 16-17 0.6875250322799289 1105 18-19 0.5849991074681071 1105 20-21 0.6786084227123581 1105 22-23 0.678432405499386 1105 24-25 0.7602298139837131 1105 26-27 0.6352956950634407 1105 28-29 0.5814685028038866 1105 30-31 0.46647056480759375 1105 32-33 0.3697691023180809 1105 34-35 0.3602464447439644 1105 36-37 0.4416810229218413 1105 38-39 0.444648497125506 1105 40-41 0.8156756189508627 1105 42-43 0.6225647502738596 1105 44-45 0.6471320850398286 1105 46-47 0.8132645597375472 1105 48-49 0.6178453476999337 1105 50-51 0.47316175077891387 1105 52-53 0.5081692141685963 1105 54-55 0.6063268910592328 1105 56-57 0.5860698901825643 1105 58-59 0.55272831721134 1105 60-61 0.4452203555427303 1105 62-63 0.516803026420007 1105 64-65 0.700005697445846 1105 66-67 0.9074681141052636 1105 68-69 0.823913992804485 1105 70-71 0.7632271987206849 1105 72-73 0.6067543976371326 1105 74-75 0.8047553800270144 1105 76-77 0.6183764882427418 1105 78-79 0.5551349930448062 1105 80-81 0.6679502685619845 1105 82-83 0.6270644275327619 1105 84-85 0.7201884794265965 1105 86-87 0.9739162673870503 1105 88-89 0.8586473065510738 1105 90-91 0.6722113492476147 1105 92-93 0.6919688371875843 1105 94-95 0.6416818601147405 1105 96-97 0.7177988261438735 1105 98-99 0.8955810999270497 1105 100-101 0.9896375467076268 1106 1 0.48767940204740157 1106 2 0.5540525567246632 1106 3 0.5410527594872647 1106 4 0.5817227360518871 1106 5 0.4186947912704042 1106 6 0.5459341823644479 1106 7 0.5935929936381115 1106 8 0.4567008858681021 1106 9 0.7536836578193444 1106 10-11 0.844093524127878 1106 12-13 0.7744421073314882 1106 14-15 0.9181326649072759 1106 16-17 0.9653419940831824 1106 18-19 0.95593501528856 1106 20-21 0.9773555546146326 1106 22-23 0.8535139391312825 1106 24-25 0.9807238867343671 1106 26-27 1.0387882888248328 1106 28-29 0.8461270076635543 1106 30-31 0.8594693849123161 1106 32-33 0.8794090847142613 1106 34-35 0.7505912634899516 1106 36-37 0.7258808946166226 1106 38-39 0.8556004339422074 1106 40-41 0.6876586609320583 1106 42-43 0.8123694907304753 1106 44-45 0.7981252602033138 1106 46-47 0.7978181863742506 1106 48-49 1.000524509577538 1106 50-51 0.941918179010699 1106 52-53 1.0067146407468286 1106 54-55 0.921136133185577 1106 56-57 0.9272041339417214 1106 58-59 1.0776980873545554 1106 60-61 1.1294785810852304 1106 62-63 1.115189689025506 1106 64-65 1.285114875907901 1106 66-67 1.3829073092128183 1106 68-69 1.3253247703206767 1106 70-71 1.4106603924157852 1106 72-73 1.3490345681329075 1106 74-75 1.3115364206421418 1106 76-77 1.304096154160863 1106 78-79 1.3411330910511943 1106 80-81 1.4172293910080143 1106 82-83 1.4935354419680102 1106 84-85 1.6244709254782812 1106 86-87 1.451950560649312 1106 88-89 1.5551181805952687 1106 90-91 1.5858240017779188 1106 92-93 1.518778004845636 1106 94-95 1.68503049810786 1106 96-97 1.6141169791347245 1106 98-99 1.705221814798307 1106 100-101 1.7898625996747306 1107 1 0.2792586868743534 1107 2 0.37539316853853677 1107 3 0.24445170897573476 1107 4 0.3345500487579116 1107 5 0.3132794177065463 1107 6 0.3636931405799757 1107 7 0.40734017923787746 1107 8 0.32182963215576166 1107 9 0.3604014103955251 1107 10-11 0.5441402761949874 1107 12-13 0.5602757857395986 1107 14-15 0.32251385306283 1107 16-17 0.48913404682545547 1107 18-19 0.5184410448210315 1107 20-21 0.5055906863441493 1107 22-23 0.6153345685180014 1107 24-25 0.5122094002724253 1107 26-27 0.4660781382831729 1107 28-29 0.41371387374633883 1107 30-31 0.5975821152775609 1107 32-33 0.5549890700801186 1107 34-35 0.5500395890980698 1107 36-37 0.6500781319405036 1107 38-39 0.6920406276590327 1107 40-41 0.6961363691012039 1107 42-43 0.6500507663049646 1107 44-45 0.7155412215074435 1107 46-47 0.8615566894472693 1107 48-49 0.7336224499634554 1107 50-51 0.6152785543333295 1107 52-53 0.7458338021186321 1107 54-55 0.8832199099214577 1107 56-57 0.8331588630988307 1107 58-59 0.6098707820694997 1107 60-61 0.5913762400682359 1107 62-63 0.7047703600883537 1107 64-65 0.8234052641281622 1107 66-67 0.7833539027681624 1107 68-69 0.9196561934981275 1107 70-71 0.7579837252593364 1107 72-73 0.7318816057586801 1107 74-75 0.7956923677277103 1107 76-77 0.866906146339165 1107 78-79 0.7739635799293971 1107 80-81 0.7662102701546623 1107 82-83 0.8453414817414036 1107 84-85 0.8500477198300374 1107 86-87 0.9048666280732149 1107 88-89 0.9174137516161096 1107 90-91 0.8711529062805177 1107 92-93 0.8101422301638834 1107 94-95 0.8476125183017835 1107 96-97 0.7052749016806494 1107 98-99 0.6768995609627808 1107 100-101 1.0896076153849101 1108 1 0.03411477483543379 1108 2 0.22429217145534963 1108 3 0.19002817631606916 1108 4 0.17131479827446583 1108 5 0.19444242771350417 1108 6 0.21775958613128665 1108 7 0.1790843717346604 1108 8 0.2116569067477485 1108 9 0.17687798751950368 1108 10-11 0.13299188250645244 1108 12-13 -0.07629184343679185 1108 14-15 0.2325788589132216 1108 16-17 0.32053049220905905 1108 18-19 0.3935921424639659 1108 20-21 0.450224510801263 1108 22-23 0.38799624783365516 1108 24-25 0.35704911744085877 1108 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-3.290429185022635 2316 100-101 -2.8916687344042913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5752.0 3 1886.0 4 2062.0 5 2164.0 6 2405.0 7 2792.0 8 3328.0 9 4293.0 10 5475.0 11 6677.0 12 8453.0 13 10327.0 14 11657.0 15 14096.0 16 17428.0 17 22188.0 18 18697.0 19 12935.0 20 12234.0 21 12992.0 22 14125.0 23 15669.0 24 17652.0 25 20246.0 26 23591.0 27 27347.0 28 32678.0 29 39097.0 30 47047.0 31 57692.0 32 71413.0 33 91497.0 34 121607.0 35 167526.0 36 239397.0 37 335386.0 38 293626.0 39 52559.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24706716416292 19.13503294358939 14.603582059781935 22.01431783246575 2 15.036379912670858 20.851418461443366 40.866199241224386 23.24600238466139 3 18.69973346574219 26.500996589857607 30.08969579751853 24.70957414688168 4 12.301516488606673 17.19833353854031 38.16627406142216 32.33387591143086 5 13.218759794676469 38.16157786340757 33.71841437134581 14.90124797057015 6 30.22394797158478 36.898249289809314 18.287864320095782 14.589938418510126 7 27.310259256628104 33.92396867033297 21.32328418120795 17.44248789183098 8 26.20048528554581 34.309471201420614 20.282387387997993 19.207656125035584 9 27.239684033267075 16.48596833282263 20.636543347804746 35.63780428610555 10-11 24.347726587462553 27.25790287698778 27.516469524059495 20.877901011490163 12-13 25.987351662073365 24.897104838390483 28.03704063159276 21.078502867943392 14-15 22.92012272503362 25.469294206347122 27.191858310440548 24.41872475817871 16-17 21.44645767652778 29.00908869305196 27.35162713337811 22.192826497042155 18-19 21.32340522778897 27.202166999612608 30.601712703932215 20.872715068666203 20-21 22.64274142464549 26.18580190010194 30.407295966450743 20.76416070880183 22-23 22.1672942067448 26.35660196466892 30.338389162309216 21.13771466627707 24-25 21.796074364990336 26.38506108955438 30.604076648073704 21.214787897381584 26-27 21.669532942479183 26.65538945669394 30.339579158805414 21.335498442021464 28-29 21.36121445667667 26.82985795002571 30.50121462471579 21.307712968581836 30-31 21.63675848048963 26.520624102036315 30.45659776700718 21.386019650466874 32-33 21.44384535485467 26.66148902954261 30.302319991523564 21.592345624079158 34-35 21.874647734662908 26.642613266854543 30.15743550834598 21.32530349013657 36-37 21.873371522968657 26.43837795490248 30.125785157963797 21.562465364165064 38-39 21.69244564788812 26.674843387167623 30.193181796625666 21.439529168318586 40-41 21.87652456542432 26.63804729865979 29.99285012695226 21.49257800896363 42-43 21.664655676142715 26.754900908577444 29.782471534456235 21.797971880823606 44-45 21.83774608315611 26.802062535092798 29.555912174246714 21.804279207504386 46-47 22.089118066956278 27.06844841745837 29.02681540541207 21.815618110173283 48-49 22.110106535043567 27.248556025401488 28.6277533069491 22.01358413260585 50-51 22.06844028996918 27.403127542493078 28.52254922971011 22.005882937827636 52-53 22.259783825087453 27.53864621070686 28.18009879339824 22.021471170807445 54-55 22.043215445027382 27.414267833436895 28.234471930609455 22.308044790926267 56-57 22.16550943678468 27.759154545607284 27.960908736647504 22.114427280960527 58-59 22.393075392292445 27.349913128033098 28.09209864839632 22.164912831278137 60-61 22.286612937276235 27.235727741097293 28.59595449614401 21.881704825482462 62-63 22.19248778829926 27.353044245614615 28.608322792726455 21.84614517335967 64-65 22.06941289895671 27.4413768772162 28.400558416101283 22.088651807725803 66-67 22.1074493278861 27.649306095722636 27.997030654508144 22.246213921883122 68-69 22.05142088591043 27.476892633365825 28.3246573155916 22.14702916513214 70-71 22.09840169380631 27.458112023667773 28.23714913867023 22.206337143855684 72-73 22.13586288822757 27.43571023177503 28.141641348090573 22.286785531906826 74-75 21.9511051915174 27.941767742886377 27.918521657332107 22.18860540826412 76-77 22.04284132192394 27.696767485663514 27.978172609052276 22.282218583360272 78-79 22.03576241217405 27.68901076623364 27.907538965396494 22.367687856195815 80-81 21.93512671904819 27.898648606538924 27.863341613180047 22.30288306123284 82-83 21.973859943151634 27.789786807797462 27.924021143601742 22.312332105449162 84-85 22.06138195726371 27.657466790219452 27.707688342339154 22.573462910177682 86-87 22.0541904498615 27.640951002553138 27.850974907713073 22.453883639872288 88-89 22.123235011218068 27.541985072215304 27.667720698827658 22.667059217738974 90-91 22.016142867712883 27.5191419090929 27.84236785910102 22.62234736409319 92-93 22.04024556413261 27.5202267312968 27.797460819813836 22.64206688475676 94-95 21.919056297454105 27.478104256512477 27.822347713813027 22.78049173222039 96-97 21.966307365316215 27.58073436280038 27.723957881495622 22.729000390387785 98-99 21.857065336919124 27.60185241179905 27.555615336824207 22.985466914457614 100-101 21.971064750160128 27.527644737263135 27.70268694534941 22.798603567227328 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1107.0 1 818.0 2 584.0 3 637.5 4 583.5 5 629.5 6 1008.5 7 2011.5 8 3357.5 9 3877.0 10 3299.5 11 2799.0 12 2835.0 13 2973.0 14 3090.5 15 3186.5 16 3356.5 17 3558.0 18 3750.0 19 3940.5 20 4251.0 21 4907.0 22 5803.0 23 6970.0 24 8322.5 25 10055.5 26 12404.0 27 14880.0 28 17147.0 29 19875.0 30 23079.0 31 26302.0 32 29690.5 33 33100.0 34 37135.0 35 40383.0 36 43954.0 37 48415.5 38 51165.5 39 52412.0 40 53934.5 41 56778.5 42 58859.5 43 61146.5 44 64419.0 45 66598.5 46 66841.0 47 68393.0 48 72890.5 49 73072.0 50 68657.5 51 65246.5 52 62816.0 53 60909.0 54 58196.0 55 53534.0 56 48417.0 57 43487.0 58 39496.5 59 35543.5 60 30545.5 61 25171.0 62 19962.0 63 15057.5 64 10981.5 65 8248.5 66 5976.5 67 4293.5 68 3034.5 69 1993.5 70 1336.0 71 904.0 72 641.0 73 467.5 74 331.0 75 243.5 76 183.0 77 101.0 78 52.0 79 32.5 80 22.5 81 24.0 82 18.5 83 14.5 84 12.0 85 8.5 86 7.5 87 5.0 88 5.0 89 2.5 90 1.5 91 2.5 92 1.5 93 0.5 94 0.5 95 1.5 96 2.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05698734144301482 2 0.09409411795296269 3 0.06679219771790615 4 0.1276798134802148 5 0.10346561041459916 6 0.09756102845900162 7 0.10947853332351043 8 0.094744163672845 9 0.11695405910215684 10-11 0.10899099903359871 12-13 0.09051886649361006 14-15 0.09417537366794798 16-17 0.10993898237509371 18-19 0.0883249621890073 20-21 0.07223633062192042 22-23 0.07516153636139075 24-25 0.062323133393715355 26-27 0.05444132904014249 28-29 0.06584421437641114 30-31 0.047507508028064645 32-33 0.049268048519412536 34-35 0.04463647276525116 36-37 0.050568139959177125 38-39 0.051380697109029996 40-41 0.06695470914787673 42-43 0.06294609387526923 44-45 0.049620156617682105 46-47 0.0371338617482763 48-49 0.04639701325659905 50-51 0.028033221669924117 52-53 0.04111539178255537 54-55 0.04731791135976564 56-57 0.0518411461606133 58-59 0.057826983831196124 60-61 0.05620186953149038 62-63 0.06362322483347996 64-65 0.04360723370877085 66-67 0.06354196911849466 68-69 0.05392670951190234 70-71 0.025812232126992934 72-73 0.02039518446130711 74-75 0.02990210311458573 76-77 0.04439270562029529 78-79 0.0640295034084064 80-81 0.042280057030677824 82-83 0.06045425194905375 84-85 0.06492331627324455 86-87 0.06684636819456302 88-89 0.09089805983020806 90-91 0.06416492960004853 92-93 0.07440314968819474 94-95 0.0836663011965175 96-97 0.09230649222328637 98-99 0.12272321486611225 100-101 0.11825415054192145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1846024.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.41779368050228 #Duplication Level Percentage of deduplicated Percentage of total 1 75.47575372926111 41.82699748048593 2 13.623250591743213 15.09940981102012 3 4.706557637479751 7.824811202977351 4 2.1010439840927955 4.657408880964602 5 1.135130991715829 3.145322754962588 6 0.6714020494355708 2.2324572151372113 7 0.4549936635034128 1.7650321478977609 8 0.310207778870401 1.3752824550021405 9 0.21393617889760094 1.0670283920648045 >10 1.1836375628698352 11.896584647851538 >50 0.0849736201129816 3.2390863266928327 >100 0.036368148954986995 3.4236650404706976 >500 0.0018620427923650238 0.6291349859628049 >1k 5.880135133784285E-4 0.4490407513760766 >5k 1.9600450445947617E-4 0.7292838908415524 >10k+ 9.800225222973809E-5 0.6394540162919601 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11774 0.6378031921578484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7893 0.42756757225258174 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5535 0.29983358829571016 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1895 0.10265305326474629 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.41704766568582E-5 7 0.0 0.0 0.0 0.0 5.41704766568582E-5 8 0.0 0.0 0.0 0.0 5.41704766568582E-5 9 0.0 0.0 0.0 0.0 5.41704766568582E-5 10-11 0.0 0.0 0.0 0.0 5.41704766568582E-5 12-13 0.0 0.0 0.0 0.0 1.895966682990037E-4 14-15 1.083409533137164E-4 0.0 0.0 0.0 2.4376714495586192E-4 16-17 1.083409533137164E-4 0.0 0.0 0.0 2.70852383284291E-4 18-19 1.354261916421455E-4 0.0 0.0 5.41704766568582E-5 2.70852383284291E-4 20-21 1.625114299705746E-4 0.0 0.0 5.41704766568582E-5 2.70852383284291E-4 22-23 1.625114299705746E-4 0.0 0.0 1.625114299705746E-4 2.9793762161272014E-4 24-25 1.625114299705746E-4 0.0 0.0 3.791933365980074E-4 3.250228599411492E-4 26-27 1.625114299705746E-4 0.0 0.0 5.687900048970111E-4 3.250228599411492E-4 28-29 2.9793762161272014E-4 0.0 0.0 0.0014084323930783132 3.250228599411492E-4 30-31 3.250228599411492E-4 0.0 0.0 0.0031148024077693463 3.521080982695783E-4 32-33 3.250228599411492E-4 0.0 0.0 0.005227450997386816 3.791933365980074E-4 34-35 3.521080982695783E-4 0.0 0.0 0.008206827213514017 4.0627857492643647E-4 36-37 3.791933365980074E-4 0.0 0.0 0.011565396766239227 4.333638132548656E-4 38-39 4.333638132548656E-4 0.0 0.0 0.014409346790724281 4.333638132548656E-4 40-41 4.333638132548656E-4 0.0 0.0 0.01722621157688091 4.333638132548656E-4 42-43 4.604490515832947E-4 0.0 0.0 0.021180656372831556 4.333638132548656E-4 44-45 4.875342899117238E-4 0.0 0.0 0.024566311163885194 4.604490515832947E-4 46-47 5.146195282401529E-4 0.0 0.0 0.030552148834468024 4.875342899117238E-4 48-49 5.41704766568582E-4 0.0 0.0 0.03735054365490373 5.41704766568582E-4 50-51 5.41704766568582E-4 0.0 0.0 0.043959341807040433 5.41704766568582E-4 52-53 5.41704766568582E-4 0.0 0.0 0.05706859715800011 5.687900048970111E-4 54-55 5.41704766568582E-4 0.0 0.0 0.08279957357000775 5.958752432254402E-4 56-57 5.958752432254402E-4 0.0 0.0 0.12540465346062674 5.958752432254402E-4 58-59 5.958752432254402E-4 0.0 0.0 0.17437476435842653 5.958752432254402E-4 60-61 6.229604815538694E-4 0.0 0.0 0.22028424332511387 5.958752432254402E-4 62-63 6.500457198822984E-4 0.0 0.0 0.2747797428419132 5.958752432254402E-4 64-65 6.500457198822984E-4 0.0 0.0 0.3423574124713438 5.958752432254402E-4 66-67 6.500457198822984E-4 0.0 0.0 0.4382662413923113 5.958752432254402E-4 68-69 6.500457198822984E-4 0.0 0.0 0.5575225457523846 5.958752432254402E-4 70-71 6.500457198822984E-4 0.0 0.0 0.664536322388008 5.958752432254402E-4 72-73 6.500457198822984E-4 0.0 0.0 0.7732564690383223 6.229604815538694E-4 74-75 6.500457198822984E-4 0.0 0.0 0.8810015471088133 6.771309582107275E-4 76-77 6.500457198822984E-4 0.0 0.0 1.0099543667904642 7.042161965391566E-4 78-79 6.500457198822984E-4 0.0 0.0 1.1190537067773767 7.042161965391566E-4 80-81 7.583866731960148E-4 0.0 0.0 1.2414248135452193 7.313014348675857E-4 82-83 7.583866731960148E-4 0.0 0.0 1.3836494000078003 8.125571498528729E-4 84-85 7.583866731960148E-4 0.0 0.0 1.5799361221739263 8.125571498528729E-4 86-87 7.583866731960148E-4 0.0 0.0 1.8240824604663861 8.39642388181302E-4 88-89 7.583866731960148E-4 0.0 0.0 2.1070690305218136 8.667276265097312E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11640 0.0 39.610672 1 GGTATCA 6665 0.0 38.5112 1 ATCAACG 14640 0.0 31.300623 3 TCAACGC 14860 0.0 30.901201 4 TATCAAC 14885 0.0 30.847628 2 CAACGCA 14930 0.0 30.690142 5 AACGCAG 15370 0.0 29.872606 6 ACGCAGA 16980 0.0 26.648277 7 CGCAGAG 16970 0.0 26.60941 8 GTACATG 10925 0.0 25.017267 1 GCAGAGT 17680 0.0 24.599842 9 GAGTACT 8435 0.0 24.083237 12-13 TACATGG 11165 0.0 23.968624 2 AGTACTT 8985 0.0 23.34944 12-13 AGAGTAC 14725 0.0 22.528929 10-11 ACATGGG 11315 0.0 22.433847 3 GTACTTT 9215 0.0 21.941292 14-15 TACTTTT 10280 0.0 20.40777 14-15 CATGGGA 7300 0.0 19.795559 4 CAGAGTA 17350 0.0 19.380606 10-11 >>END_MODULE