FastQCFastQC Report
Thu 26 May 2016
SRR937127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846024
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120720.6539459942015922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89380.48417572035899853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63310.3429532877145693No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20800.11267459144626506No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19520.1057407704341872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75300.037.350361
GTATCAA120800.035.788421
ATCAACG155250.027.658113
TATCAAC156150.027.5602822
TCAACGC156100.027.5075054
CAACGCA158450.027.128785
AACGCAG161300.026.6494436
GTACATG104050.024.838471
TACATGG105950.024.3442442
GTATAGG5500.024.185961
TATCACG1000.001653938823.750212
ACGCAGA184800.023.1063657
ACATGGG106950.022.9612463
CGCAGAG185100.022.9149518
CATGGGG39850.022.4086274
GAGTACT100000.022.18059312-13
AGTACTT104950.022.1526912-13
GCAGAGT193100.021.8180169
AGAGTAC158900.021.62632210-11
GTACTTT109100.020.24317414-15
Produced by FastQC (version 0.11.5)