##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937127_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1846024 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.950494143088065 31.0 31.0 34.0 30.0 34.0 2 31.92706757875304 31.0 31.0 34.0 30.0 34.0 3 32.107671947927 33.0 31.0 34.0 30.0 34.0 4 35.88493486541887 37.0 35.0 37.0 35.0 37.0 5 35.79831898176838 37.0 35.0 37.0 35.0 37.0 6 35.83923231767301 37.0 35.0 37.0 35.0 37.0 7 35.76635785883607 37.0 35.0 37.0 33.0 37.0 8 35.74950921548149 37.0 35.0 37.0 33.0 37.0 9 37.38294139187789 39.0 37.0 39.0 34.0 39.0 10-11 37.305124418750786 39.0 37.0 39.0 34.0 39.0 12-13 37.20270159001183 39.0 37.0 39.0 33.0 39.0 14-15 38.327638752258906 40.0 38.0 41.0 33.0 41.0 16-17 38.25228761922922 40.0 38.0 41.0 33.0 41.0 18-19 38.337920308728386 40.0 38.0 41.0 33.5 41.0 20-21 38.4293836374825 40.0 38.0 41.0 34.0 41.0 22-23 38.353713711197685 40.0 38.0 41.0 34.0 41.0 24-25 38.24829065060909 40.0 38.0 41.0 33.0 41.0 26-27 38.0738506108263 40.0 37.0 41.0 33.0 41.0 28-29 37.88618647428203 40.0 37.0 41.0 33.0 41.0 30-31 37.71525830650089 40.0 37.0 41.0 33.0 41.0 32-33 37.651953333217776 40.0 36.5 41.0 33.0 41.0 34-35 37.55297249656559 40.0 36.0 41.0 33.0 41.0 36-37 37.3717058933145 40.0 36.0 41.0 32.0 41.0 38-39 37.205981341521024 40.0 36.0 41.0 31.5 41.0 40-41 36.996775502377005 39.0 36.0 41.0 31.0 41.0 42-43 36.740504998851584 39.0 35.0 41.0 31.0 41.0 44-45 36.37032752553596 39.0 35.0 41.0 30.0 41.0 46-47 36.021764884963574 39.0 35.0 41.0 29.5 41.0 48-49 35.64869958353738 39.0 35.0 40.0 28.0 41.0 50-51 35.375186075587315 39.0 35.0 40.0 27.0 41.0 52-53 35.105607511061606 38.0 34.5 40.0 26.0 41.0 54-55 34.86020604282501 38.0 34.0 40.0 26.0 41.0 56-57 34.58214275654054 38.0 34.0 40.0 25.5 41.0 58-59 34.24737462784883 38.0 34.0 40.0 24.0 41.0 60-61 33.91491389061031 37.0 33.0 40.0 23.5 41.0 62-63 33.54422450629028 37.0 33.0 39.5 23.0 41.0 64-65 33.14160189683341 36.0 32.0 39.0 22.0 40.5 66-67 32.75415243788813 36.0 32.0 39.0 21.5 40.0 68-69 32.3112960611563 35.0 31.0 38.5 20.0 40.0 70-71 31.836809813956915 35.0 31.0 38.0 18.5 40.0 72-73 31.49462628871564 35.0 31.0 37.0 18.0 39.0 74-75 31.052145584239426 35.0 30.5 37.0 15.5 39.0 76-77 29.6502206905219 33.5 28.5 35.0 13.0 37.5 78-79 30.373687720202987 34.0 30.0 36.0 12.0 38.0 80-81 30.206830734595002 34.0 30.0 35.5 7.5 37.0 82-83 29.867987902649155 34.0 30.0 35.0 7.0 37.0 84-85 29.484521598852453 34.0 29.0 35.0 2.0 36.5 86-87 29.10539299597405 34.0 29.0 35.0 2.0 36.0 88-89 28.726528203316967 34.0 29.0 35.0 2.0 36.0 90-91 28.28473085940378 33.5 29.0 35.0 2.0 35.0 92-93 27.80410953487062 33.0 27.0 35.0 2.0 35.0 94-95 27.306725968893147 33.0 26.5 35.0 2.0 35.0 96-97 26.662424215503158 33.0 25.0 35.0 2.0 35.0 98-99 25.88467809735951 32.0 24.5 34.0 2.0 35.0 100-101 22.9097029616083 29.0 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29892907068311914 1101 2 0.24904651154161073 1101 3 0.19639320043047803 1101 4 0.2103486927803786 1101 5 0.247408470478085 1101 6 0.21210542975834557 1101 7 0.33416432101795834 1101 8 0.28936862698846966 1101 9 0.3495262251895639 1101 10-11 0.3774657584535035 1101 12-13 0.36660747233934643 1101 14-15 0.43019256570767084 1101 16-17 0.5473621339642349 1101 18-19 0.5335816287691841 1101 20-21 0.4384779595113457 1101 22-23 0.4144910093710905 1101 24-25 0.4145213819179503 1101 26-27 0.4015428458700896 1101 28-29 0.5133397304626612 1101 30-31 0.3894502124914041 1101 32-33 0.5709220828616353 1101 34-35 0.5829107292324096 1101 36-37 0.6059246599953454 1101 38-39 0.5398853147134801 1101 40-41 0.4409393799699899 1101 42-43 0.6762680807650128 1101 44-45 0.6989979092768763 1101 46-47 0.6127521576388588 1101 48-49 0.4299185025390386 1101 50-51 0.5173847364810058 1101 52-53 0.3448498649966538 1101 54-55 0.2460685611598521 1101 56-57 0.3163907398104939 1101 58-59 0.5027304047762158 1101 60-61 0.542923797896357 1101 62-63 0.6814957423668417 1101 64-65 0.5368180819700967 1101 66-67 0.7194571398499647 1101 68-69 0.5206431821606898 1101 70-71 0.5184637192364114 1101 72-73 0.640976123971253 1101 74-75 0.6647083757389893 1101 76-77 0.3365909808061325 1101 78-79 0.5179206942620027 1101 80-81 0.8684997011766633 1101 82-83 0.8045695967402544 1101 84-85 0.8498673036294768 1101 86-87 0.6967999964490481 1101 88-89 0.6007125203997141 1101 90-91 0.7253443014031156 1101 92-93 0.8326663179789158 1101 94-95 0.8781040494552244 1101 96-97 0.9152481614756383 1101 98-99 1.0389714455100965 1101 100-101 1.1742137699365216 1102 1 0.28159895532091284 1102 2 0.1123172259415206 1102 3 0.22240964611948044 1102 4 0.27975166630947257 1102 5 0.2746116719468006 1102 6 0.24487336195404197 1102 7 0.3096492084147542 1102 8 0.3178427078812476 1102 9 0.36926272801817817 1102 10-11 0.34874591404034305 1102 12-13 0.3520487637049712 1102 14-15 0.4999834427079506 1102 16-17 0.38998291102175386 1102 18-19 0.41267951388142876 1102 20-21 0.4686208149965907 1102 22-23 0.4177381498118038 1102 24-25 0.5127283235045539 1102 26-27 0.35646191956885076 1102 28-29 0.40903398581691874 1102 30-31 0.3074124155123812 1102 32-33 0.4805125328618942 1102 34-35 0.5421929514890067 1102 36-37 0.6213959973614536 1102 38-39 0.6516571133736804 1102 40-41 0.6580995720168517 1102 42-43 0.6943574039596712 1102 44-45 0.6994310976983655 1102 46-47 0.6611970627757486 1102 48-49 0.48338778097089374 1102 50-51 0.6392583021889848 1102 52-53 0.7295076461436807 1102 54-55 0.6191190521067327 1102 56-57 0.6846279723553224 1102 58-59 0.7040249097295188 1102 60-61 0.6775595006156436 1102 62-63 0.7242592395382204 1102 64-65 0.7399156048027393 1102 66-67 0.8344291824365158 1102 68-69 0.6687219077100366 1102 70-71 0.5556189734509793 1102 72-73 0.5449146576284427 1102 74-75 0.35244737069028176 1102 76-77 0.5698418342904645 1102 78-79 0.6745175028494472 1102 80-81 0.5838576811262293 1102 82-83 0.8754745479807688 1102 84-85 0.8964676125340745 1102 86-87 0.5469266331535145 1102 88-89 0.8521580933687574 1102 90-91 0.9888289025861354 1102 92-93 0.7821115531641283 1102 94-95 0.9422241548128341 1102 96-97 1.0495489811815837 1102 98-99 1.0281222350475652 1102 100-101 1.3798907622164869 1103 1 0.12774599896167516 1103 2 -0.10129877370108176 1103 3 0.15693978865519398 1103 4 0.22483681411901557 1103 5 0.1895608629408656 1103 6 0.19112955415342725 1103 7 0.2603490657903009 1103 8 0.25911668300321367 1103 9 0.18905255201880777 1103 10-11 0.3031503047071169 1103 12-13 0.41031701946693744 1103 14-15 0.27596115155516543 1103 16-17 0.4536391337594736 1103 18-19 0.4708334803620744 1103 20-21 0.3227775941869453 1103 22-23 0.47473254673006693 1103 24-25 0.4679321271497585 1103 26-27 0.3742156195887105 1103 28-29 0.43158263373035766 1103 30-31 0.5896071858674219 1103 32-33 0.6236401975080739 1103 34-35 0.5044577519509446 1103 36-37 0.605227659500919 1103 38-39 0.777949064195333 1103 40-41 0.6489882530328686 1103 42-43 0.82267642129829 1103 44-45 0.9734931223536378 1103 46-47 1.0246747677980252 1103 48-49 0.826315294026962 1103 50-51 0.6143536427052538 1103 52-53 0.7334178083426579 1103 54-55 0.8406728783802961 1103 56-57 0.8909026833962201 1103 58-59 0.9621840475324035 1103 60-61 0.9908602331992284 1103 62-63 0.8660916256627971 1103 64-65 0.7849847490529456 1103 66-67 0.9236375509210717 1103 68-69 0.911547310101632 1103 70-71 0.9223166680510424 1103 72-73 0.9962590943108047 1103 74-75 0.9691154602355496 1103 76-77 0.8777085475908315 1103 78-79 0.818511287470912 1103 80-81 1.0240146200461808 1103 82-83 0.9408945281951091 1103 84-85 0.715687061112007 1103 86-87 0.6726286248469648 1103 88-89 0.9988059290786033 1103 90-91 1.1895831506437524 1103 92-93 1.1755524805994604 1103 94-95 1.1237449091037348 1103 96-97 1.4458130221629375 1103 98-99 1.4059678010059429 1103 100-101 1.664285180493227 1104 1 0.260154806006188 1104 2 0.10130022260984717 1104 3 0.14985719224053184 1104 4 0.1609334075104627 1104 5 0.12535665333432888 1104 6 0.28622097490146814 1104 7 0.3162537410281985 1104 8 0.24504990824215866 1104 9 0.3169288711713705 1104 10-11 0.32456704010093773 1104 12-13 0.32050089479081834 1104 14-15 0.3454126212260462 1104 16-17 0.3278677054884369 1104 18-19 0.3861232675454147 1104 20-21 0.24220108625859638 1104 22-23 0.32096249222339424 1104 24-25 0.35880737042052857 1104 26-27 0.24946779763605065 1104 28-29 0.38140854764579046 1104 30-31 0.46335534892484276 1104 32-33 0.5333204468416355 1104 34-35 0.5115981726312384 1104 36-37 0.49219567625710425 1104 38-39 0.49778757679167285 1104 40-41 0.6488158023820887 1104 42-43 0.624983726313701 1104 44-45 0.6781514510337772 1104 46-47 0.9311460624307628 1104 48-49 0.9754369200686668 1104 50-51 0.7424777592789766 1104 52-53 0.8792879876095654 1104 54-55 0.8594394809535331 1104 56-57 0.7544469078618405 1104 58-59 0.6180331472261145 1104 60-61 0.7408396442561624 1104 62-63 0.834533010548121 1104 64-65 0.6982950772845058 1104 66-67 0.7756608745430356 1104 68-69 0.8353174054941519 1104 70-71 0.8439450753020914 1104 72-73 0.9358534716894873 1104 74-75 0.835669758973232 1104 76-77 0.819299322342534 1104 78-79 0.7774284609273536 1104 80-81 0.8386425822256065 1104 82-83 0.9732435542942959 1104 84-85 1.170387443803513 1104 86-87 1.0153550915390177 1104 88-89 1.2008596834504708 1104 90-91 1.302922361803951 1104 92-93 1.5422014008627194 1104 94-95 1.4522282078203865 1104 96-97 1.569571260660581 1104 98-99 1.6847625330401215 1104 100-101 1.3755921315540398 1105 1 0.22941976475247472 1105 2 0.07284622198043778 1105 3 0.10433443998628888 1105 4 0.13493604373437051 1105 5 0.0522276653615279 1105 6 0.09747954376940271 1105 7 0.16400325870868926 1105 8 0.12980348059920743 1105 9 0.2410906842366458 1105 10-11 0.4221024786126151 1105 12-13 0.4041026585067087 1105 14-15 0.45206595094103363 1105 16-17 0.4024188518809595 1105 18-19 0.4179643369948636 1105 20-21 0.38016812661836497 1105 22-23 0.35612877273039345 1105 24-25 0.3904360710540473 1105 26-27 0.34611613312062417 1105 28-29 0.38267677430631863 1105 30-31 0.5119119821293978 1105 32-33 0.38767135652131657 1105 34-35 0.3645443124788059 1105 36-37 0.234264484893707 1105 38-39 0.3171617700752307 1105 40-41 0.11452009149101627 1105 42-43 0.21959531657699927 1105 44-45 0.35773251719934507 1105 46-47 0.2477198379321237 1105 48-49 0.2913017543012657 1105 50-51 0.22223829847342103 1105 52-53 0.22589213897807525 1105 54-55 0.2749457682107206 1105 56-57 0.20621125088108982 1105 58-59 0.10228564320108546 1105 60-61 0.2806774685960818 1105 62-63 0.16087974240583236 1105 64-65 0.17473895718580224 1105 66-67 0.3169881311826259 1105 68-69 0.07051601167057697 1105 70-71 0.13221336581579024 1105 72-73 0.10811166199931321 1105 74-75 0.1276854326470236 1105 76-77 0.1664308325157151 1105 78-79 0.24724101765405848 1105 80-81 0.3717533036296494 1105 82-83 0.5619524317098872 1105 84-85 0.48177058337287093 1105 86-87 0.5238880420573686 1105 88-89 0.3721925728522102 1105 90-91 0.5324491527714663 1105 92-93 0.5035135037757925 1105 94-95 0.5064874425857013 1105 96-97 0.6590483715781055 1105 98-99 0.7388347692238888 1105 100-101 0.7923193257087391 1106 1 0.42802549844529025 1106 2 0.3584864753924002 1106 3 0.29628899479099147 1106 4 0.2777253454475641 1106 5 0.2836736138892846 1106 6 0.3402767195896672 1106 7 0.28839156572072966 1106 8 0.3681252337050509 1106 9 0.4484458327123946 1106 10-11 0.435515775843065 1106 12-13 0.4913912555178399 1106 14-15 0.6397277440033946 1106 16-17 0.6917854196431463 1106 18-19 0.6189472575072799 1106 20-21 0.5623415449757729 1106 22-23 0.47901336945097484 1106 24-25 0.5529454330011063 1106 26-27 0.521286784553439 1106 28-29 0.6240508120934862 1106 30-31 0.6702549342847419 1106 32-33 0.6778805758188611 1106 34-35 0.6618441957100742 1106 36-37 0.5834733507720316 1106 38-39 0.6968061961535383 1106 40-41 0.7351958180605038 1106 42-43 0.7774899939858244 1106 44-45 0.719810054569308 1106 46-47 0.6672684379586187 1106 48-49 0.6847742416218168 1106 50-51 0.7127742626944524 1106 52-53 0.7459671444258547 1106 54-55 0.8416052728988674 1106 56-57 0.8081478234226793 1106 58-59 0.6696901360811154 1106 60-61 0.6855585604234236 1106 62-63 0.828304164711902 1106 64-65 0.9601309320667966 1106 66-67 0.8468944781402001 1106 68-69 0.8485861351719777 1106 70-71 0.8036561769086354 1106 72-73 0.9826382236819562 1106 74-75 0.8778377264232446 1106 76-77 0.9255733422561079 1106 78-79 0.9056903312943945 1106 80-81 0.6868247107936192 1106 82-83 0.8338644236087092 1106 84-85 0.7958473504232089 1106 86-87 0.8429044379015522 1106 88-89 1.0762772201135782 1106 90-91 1.279923487149997 1106 92-93 1.1847186816421065 1106 94-95 1.3321582218964565 1106 96-97 1.609803614421061 1106 98-99 1.582491626625032 1106 100-101 1.827726560487143 1107 1 0.1717000921846683 1107 2 0.10741332992816766 1107 3 0.12844343393686586 1107 4 0.14974133910709497 1107 5 0.2010201149794213 1107 6 0.11427129041310735 1107 7 0.14051403535786022 1107 8 0.14552483920665793 1107 9 0.2119145095695245 1107 10-11 0.23303331528919813 1107 12-13 0.22871320806226692 1107 14-15 0.2893756193074637 1107 16-17 0.28927090052896887 1107 18-19 0.2990049035481235 1107 20-21 0.322202001186902 1107 22-23 0.2501180688500284 1107 24-25 0.2245492663737707 1107 26-27 0.1972238666604582 1107 28-29 0.16245830311041232 1107 30-31 0.2058541844706383 1107 32-33 0.3618756364258715 1107 34-35 0.3260934094590411 1107 36-37 0.33878535032083334 1107 38-39 0.20757564612660673 1107 40-41 0.25525675900893674 1107 42-43 0.2555232013112061 1107 44-45 0.29130049562682103 1107 46-47 0.14929768545811584 1107 48-49 0.17075678454838084 1107 50-51 0.16715656399446033 1107 52-53 0.20265691997141033 1107 54-55 0.1799327659342964 1107 56-57 0.22897045201683142 1107 58-59 0.1499678878855235 1107 60-61 0.21734581031842737 1107 62-63 0.14956611431762212 1107 64-65 0.20888606551797295 1107 66-67 0.03360605647515058 1107 68-69 0.20028175101543866 1107 70-71 0.25440977570466217 1107 72-73 0.13464715258467308 1107 74-75 0.15357726249431636 1107 76-77 0.14961533303333496 1107 78-79 0.1727139462829541 1107 80-81 0.16618552840564504 1107 82-83 0.28132445059133104 1107 84-85 0.346733636038266 1107 86-87 0.23542945025745254 1107 88-89 0.30381240660979714 1107 90-91 0.6504483124976339 1107 92-93 0.6859265175853899 1107 94-95 0.7094411698591756 1107 96-97 0.6332982349649576 1107 98-99 0.6848268552723233 1107 100-101 0.8311023565709519 1108 1 0.05512276179782205 1108 2 -0.10751182755524624 1108 3 0.030989356654856692 1108 4 0.06250165599610114 1108 5 0.046769062617400436 1108 6 0.10345676285044902 1108 7 0.09166224843875881 1108 8 0.14860471551622823 1108 9 0.06716135506356125 1108 10-11 0.08700881199245458 1108 12-13 0.018649621271912054 1108 14-15 0.048200044168879685 1108 16-17 0.19302637842323378 1108 18-19 0.0790993613446318 1108 20-21 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-1.291219138778139 2316 92-93 -1.615827999147708 2316 94-95 -1.5585405016720628 2316 96-97 -1.3340403186343046 2316 98-99 -1.2118422175289432 2316 100-101 -1.181835193774802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 24.0 5 51.0 6 132.0 7 326.0 8 699.0 9 1336.0 10 2274.0 11 3663.0 12 5472.0 13 7345.0 14 9292.0 15 12890.0 16 16939.0 17 22087.0 18 19301.0 19 11737.0 20 10372.0 21 10792.0 22 11661.0 23 12778.0 24 14917.0 25 17489.0 26 21413.0 27 26522.0 28 32962.0 29 40843.0 30 50467.0 31 61870.0 32 76998.0 33 98088.0 34 129771.0 35 178943.0 36 261522.0 37 378186.0 38 275226.0 39 21628.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.858799161971675 19.929286619448565 15.330424686619773 21.88148953195999 2 15.31624990993851 21.612103397634076 39.9630328051351 23.10861388729231 3 18.90862187956736 26.39636008663159 30.447166759300494 24.24785127450056 4 12.736481728258633 17.819254395943705 37.63632029988841 31.807943575909253 5 13.224925907327142 38.32770223602505 33.77537040919307 14.672001447454738 6 30.316280772993547 37.22228060614793 17.796088662190726 14.665349958667791 7 27.21287871277877 34.036737353759946 21.15244501287362 17.597938920587662 8 26.324522324736837 34.59635411023908 19.979588564395694 19.09953500062838 9 26.94244495196162 16.418475599450495 20.558941812240793 36.08013763634709 10-11 24.430328799076502 27.43614509260729 27.285171728815655 20.84835437950056 12-13 25.9827650464568 24.974539765635832 28.025217549577036 21.017477638330327 14-15 22.80029945439496 25.61924980390288 27.243307779313813 24.337142962388356 16-17 21.285313733732604 29.057043678738737 27.506982574441068 22.150660013087588 18-19 21.201241153961163 27.139815083660885 30.86761060527924 20.791333157098716 20-21 22.43772902751892 26.06395124119406 30.833222055764825 20.665097675522194 22-23 22.001772457996214 26.25713425177571 30.760542658166955 20.98055063206112 24-25 21.690256438037068 26.338509043385226 30.9706724811582 21.000562037419503 26-27 21.616670061408715 26.575250596969003 30.73705086479248 21.071028476829802 28-29 21.288983304951632 26.677136340231527 30.874124747117566 21.159755607699275 30-31 21.571446373231044 26.35854842554667 30.802911600892873 21.26709360032941 32-33 21.38339874914105 26.50974817870853 30.692929052717172 21.413924019433246 34-35 21.76834191638224 26.539646751988844 30.56194705965682 21.130064271972095 36-37 21.884679933129572 26.25577071634349 30.509740741663677 21.349808608863256 38-39 21.620422523194303 26.578083258147494 30.539715593032756 21.261778625625446 40-41 21.76646410583183 26.50831077265198 30.37123211792318 21.353993003593004 42-43 21.652519269559313 26.568792807511887 30.14269102913545 21.635996893793354 44-45 21.83894596682365 26.664938619100397 29.833170279315326 21.662945134760626 46-47 22.083738370611208 26.937658160906402 29.317015736798584 21.661587731683806 48-49 22.083813307497564 27.18752429213234 28.850182785176433 21.878479615193662 50-51 22.013096813877844 27.33705972546907 28.75129027478824 21.898553185864845 52-53 22.304041395259013 27.411601842570064 28.43340103796922 21.850955724201704 54-55 22.105737305732323 27.321258026263685 28.410305470670995 22.162699197333 56-57 22.169282518801932 27.62740175420587 28.17872282566743 22.02459290132477 58-59 22.442755452245404 27.269393292706873 28.28382689000656 22.004024365041168 60-61 22.31966075968293 27.18800632933678 28.69704719429558 21.795285716684713 62-63 22.189283562943928 27.2831230796566 28.79428978171465 21.733303575684822 64-65 22.15616431751817 27.339007331163444 28.565183487642493 21.939644863675895 66-67 22.16250826777348 27.567072566268138 28.206880412649248 22.063538753309142 68-69 22.119897173573356 27.4216715009316 28.417000108611834 22.041431216883208 70-71 22.13918476550375 27.378534697059244 28.384482083356584 22.097798454080426 72-73 22.20512842736606 27.40265023639996 28.24020706122997 22.15201427500401 74-75 22.049222176929526 27.823273614686748 27.98868374758094 22.138820460802783 76-77 22.082812952413143 27.645207606594465 28.112704011666285 22.159275429326108 78-79 22.008199818365654 27.590110922627183 28.072636403845795 22.329052855161372 80-81 21.92670980170469 27.752729888832516 28.026741303807857 22.29381900565494 82-83 22.00130716227962 27.639846184974026 28.084622641534985 22.27422401121137 84-85 22.030580459925783 27.62244372816165 27.836344429697448 22.510631382215117 86-87 22.091565972663307 27.53975461820967 27.895663036527203 22.47301637259982 88-89 22.094787286974814 27.47128718824765 27.764760117367505 22.669165407410034 90-91 21.955994458340726 27.457946633767015 27.946322285677137 22.63973662221512 92-93 22.00303029810571 27.470784491850857 27.867339681033226 22.6588455290102 94-95 21.837502038171912 27.42539749092235 27.82471667379011 22.91238379711563 96-97 21.91153953559299 27.523356727303554 27.75821734534099 22.80688639176246 98-99 21.766501531719182 27.647815641141626 27.540392152613947 23.045290674525248 100-101 21.563927320949304 27.69286023640762 27.681078046041552 23.06213439660153 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1519.0 1 1037.0 2 605.5 3 667.5 4 591.5 5 580.0 6 1013.0 7 2151.5 8 3501.5 9 4019.0 10 3396.0 11 2741.5 12 2714.0 13 2840.5 14 3047.5 15 3300.0 16 3525.0 17 3724.0 18 3957.0 19 4205.5 20 4489.0 21 5063.0 22 6045.5 23 7182.0 24 8447.0 25 10279.5 26 12611.0 27 15126.5 28 17547.5 29 20100.0 30 23249.0 31 26461.5 32 29677.5 33 32844.5 34 36578.5 35 39933.0 36 43821.5 37 48551.5 38 51213.0 39 52512.5 40 53928.0 41 56743.5 42 59271.0 43 61623.5 44 64555.0 45 66602.0 46 67010.5 47 68519.0 48 73206.0 49 73385.0 50 68726.0 51 65270.5 52 62928.5 53 61005.5 54 58108.5 55 53203.0 56 48007.5 57 43212.5 58 39241.0 59 35211.5 60 30007.5 61 24654.0 62 19574.0 63 14579.5 64 10581.0 65 7975.5 66 5593.5 67 4087.5 68 3073.5 69 1949.0 70 1242.5 71 818.5 72 573.5 73 477.0 74 350.5 75 256.5 76 192.5 77 88.5 78 34.0 79 18.5 80 10.0 81 6.5 82 3.5 83 2.0 84 2.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014246835360753706 2 0.0034127400293820667 3 0.0024918419262154774 4 2.1668190662743278E-4 5 0.001354261916421455 6 3.2502285994114917E-4 7 5.4170476656858194E-5 8 0.0 9 0.0 10-11 1.6251142997057458E-4 12-13 4.3336381325486555E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002139733827945899 22-23 0.0 24-25 0.003331484314396779 26-27 0.0017334552530194622 28-29 0.0031148024077693468 30-31 0.016467824903684893 32-33 6.771309582107275E-4 34-35 0.002193904304602757 36-37 0.003575251459352641 38-39 0.0023022452579164734 40-41 0.004144041464249652 42-43 0.0023022452579164734 44-45 5.41704766568582E-4 46-47 2.7085238328429097E-5 48-49 2.4376714495586192E-4 50-51 1.354261916421455E-4 52-53 8.39642388181302E-4 54-55 0.0028710352628134846 56-57 1.895966682990037E-4 58-59 0.0010021538181518767 60-61 9.208981031665894E-4 62-63 0.0 64-65 2.7085238328429097E-5 66-67 5.4170476656858194E-5 68-69 2.7085238328429097E-5 70-71 1.6251142997057458E-4 72-73 0.0 74-75 3.521080982695783E-4 76-77 0.001110494771465593 78-79 4.062785749264365E-4 80-81 0.006256690053867122 82-83 0.002193904304602757 84-85 0.004008615272607507 86-87 0.0023564157345733316 88-89 0.0014896881080636005 90-91 3.521080982695783E-4 92-93 5.4170476656858194E-5 94-95 3.791933365980074E-4 96-97 0.001923051921318466 98-99 0.0031960581227546336 100-101 9.479833414950185E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1846024.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.23589426127996 #Duplication Level Percentage of deduplicated Percentage of total 1 73.65168862842624 39.209135079876155 2 14.602350781558574 15.547384043463422 3 5.037170451023776 8.044748205202369 4 2.278452805424584 4.851818905155994 5 1.2133847109562312 3.229781008535984 6 0.7183186598279298 2.29442017323024 7 0.4728223900726792 1.7619785933592373 8 0.3354358700808424 1.4285782808851335 9 0.25223619637134836 1.2085217531003294 >10 1.3019620991732743 12.57554965894676 >50 0.09130590034571305 3.3511462174570696 >100 0.04190798691168315 3.758779599272178 >500 0.0020438067773131063 0.7193568624547907 >1k 6.131420331939319E-4 0.5313981571850862 >5k 2.0438067773131063E-4 0.8306632332165318 >10k+ 1.0219033886565531E-4 0.6567402286587184 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12072 0.6539459942015922 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8938 0.48417572035899853 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6331 0.3429532877145693 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2080 0.11267459144626506 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1952 0.1057407704341872 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.083409533137164E-4 0.0 0.0 0.0 0.0 6 1.083409533137164E-4 0.0 0.0 0.0 0.0 7 1.083409533137164E-4 0.0 0.0 0.0 0.0 8 1.083409533137164E-4 0.0 0.0 0.0 0.0 9 1.083409533137164E-4 0.0 0.0 0.0 5.41704766568582E-5 10-11 1.083409533137164E-4 0.0 0.0 0.0 5.41704766568582E-5 12-13 1.083409533137164E-4 0.0 0.0 0.0 5.41704766568582E-5 14-15 1.625114299705746E-4 0.0 0.0 5.41704766568582E-5 1.354261916421455E-4 16-17 1.625114299705746E-4 0.0 0.0 5.41704766568582E-5 1.625114299705746E-4 18-19 2.166819066274328E-4 0.0 0.0 1.083409533137164E-4 1.625114299705746E-4 20-21 2.166819066274328E-4 0.0 0.0 1.083409533137164E-4 1.625114299705746E-4 22-23 2.166819066274328E-4 0.0 0.0 2.166819066274328E-4 1.625114299705746E-4 24-25 2.70852383284291E-4 0.0 0.0 4.3336381325486555E-4 1.625114299705746E-4 26-27 2.70852383284291E-4 0.0 0.0 6.229604815538694E-4 1.625114299705746E-4 28-29 3.791933365980074E-4 0.0 0.0 0.0014355176314067423 1.625114299705746E-4 30-31 4.333638132548656E-4 0.0 0.0 0.0031689728844262045 2.166819066274328E-4 32-33 4.333638132548656E-4 0.0 0.0 0.005308706712372104 2.70852383284291E-4 34-35 4.604490515832947E-4 0.0 0.0 0.008342253405156163 2.70852383284291E-4 36-37 4.875342899117238E-4 5.41704766568582E-5 0.0 0.011592482004567655 2.70852383284291E-4 38-39 4.875342899117238E-4 5.41704766568582E-5 0.0 0.014409346790724281 2.70852383284291E-4 40-41 4.875342899117238E-4 5.41704766568582E-5 0.0 0.01717204110022405 2.70852383284291E-4 42-43 4.875342899117238E-4 5.41704766568582E-5 0.0 0.02107231541951784 2.9793762161272014E-4 44-45 4.875342899117238E-4 5.41704766568582E-5 0.0 0.024512140687228335 3.521080982695783E-4 46-47 5.41704766568582E-4 5.41704766568582E-5 0.0 0.030606319311124883 4.0627857492643647E-4 48-49 6.500457198822984E-4 5.41704766568582E-5 0.0 0.03729637317824687 4.333638132548656E-4 50-51 6.500457198822984E-4 5.41704766568582E-5 0.0 0.04382391561539828 4.333638132548656E-4 52-53 6.500457198822984E-4 5.41704766568582E-5 0.0 0.056716489059730535 5.146195282401529E-4 54-55 6.500457198822984E-4 5.41704766568582E-5 0.0 0.08220369832678232 5.41704766568582E-4 56-57 6.500457198822984E-4 5.41704766568582E-5 0.0 0.12456501107244544 5.687900048970111E-4 58-59 6.500457198822984E-4 5.41704766568582E-5 0.0 0.1735080367319168 5.958752432254402E-4 60-61 6.500457198822984E-4 5.41704766568582E-5 0.0 0.219850879511859 5.958752432254402E-4 62-63 7.042161965391566E-4 5.41704766568582E-5 0.0 0.2744818052203005 5.958752432254402E-4 64-65 7.042161965391566E-4 5.41704766568582E-5 0.0 0.3416802815131331 6.229604815538694E-4 66-67 7.042161965391566E-4 5.41704766568582E-5 0.0 0.43756202519577214 7.042161965391566E-4 68-69 7.583866731960148E-4 5.41704766568582E-5 0.0 0.5568995852708307 7.042161965391566E-4 70-71 7.583866731960148E-4 5.41704766568582E-5 0.0 0.6644550666730227 7.042161965391566E-4 72-73 7.583866731960148E-4 5.41704766568582E-5 0.0 0.7737440033282341 7.854719115244439E-4 74-75 7.583866731960148E-4 5.41704766568582E-5 0.0 0.8817870190203378 8.667276265097312E-4 76-77 7.583866731960148E-4 5.41704766568582E-5 0.0 1.0120670153800817 8.667276265097312E-4 78-79 7.583866731960148E-4 5.41704766568582E-5 0.0 1.1216268044185775 8.667276265097312E-4 80-81 8.667276265097312E-4 5.41704766568582E-5 0.0 1.2445396159529887 8.938128648381603E-4 82-83 8.667276265097312E-4 5.41704766568582E-5 0.0 1.3878205267103785 9.208981031665894E-4 84-85 8.938128648381603E-4 5.41704766568582E-5 0.0 1.584838550311372 9.208981031665894E-4 86-87 9.208981031665894E-4 5.41704766568582E-5 0.0 1.8303391505202533 9.208981031665894E-4 88-89 9.208981031665894E-4 5.41704766568582E-5 0.0 2.1143820448704895 9.208981031665894E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7530 0.0 37.35036 1 GTATCAA 12080 0.0 35.78842 1 ATCAACG 15525 0.0 27.65811 3 TATCAAC 15615 0.0 27.560282 2 TCAACGC 15610 0.0 27.507505 4 CAACGCA 15845 0.0 27.12878 5 AACGCAG 16130 0.0 26.649443 6 GTACATG 10405 0.0 24.83847 1 TACATGG 10595 0.0 24.344244 2 GTATAGG 550 0.0 24.18596 1 TATCACG 100 0.0016539388 23.75021 2 ACGCAGA 18480 0.0 23.106365 7 ACATGGG 10695 0.0 22.961246 3 CGCAGAG 18510 0.0 22.914951 8 CATGGGG 3985 0.0 22.408627 4 GAGTACT 10000 0.0 22.180593 12-13 AGTACTT 10495 0.0 22.15269 12-13 GCAGAGT 19310 0.0 21.818016 9 AGAGTAC 15890 0.0 21.626322 10-11 GTACTTT 10910 0.0 20.243174 14-15 >>END_MODULE