FastQCFastQC Report
Thu 26 May 2016
SRR937126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750380
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111120.6348335789942755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76950.4396188256264354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52580.30039191489847916No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19140.10934768450279368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114950.038.3626441
GGTATCA67750.036.121641
ATCAACG143150.030.5721073
TCAACGC143650.030.4987754
TATCAAC143950.030.4030722
CAACGCA145050.030.1785475
AACGCAG148500.029.4757446
CGCGGAA1200.027.70767482-83
ACGCAGA164000.026.3711557
GTACATG103750.026.0611571
CGCAGAG164550.026.053448
TACATGG104400.025.5347652
ACATGGG106050.024.195813
GCAGAGT171700.024.1103959
GAGTACT83950.023.0672112-13
AGAGTAC138600.021.82594110-11
AGTACTT88900.021.32844712-13
GTACTTT91000.021.25250414-15
CCGTATG1150.003711009420.6646759
CATGGGG41100.020.0014464
Produced by FastQC (version 0.11.5)