##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937126_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1750380 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.538927547161187 31.0 31.0 34.0 30.0 34.0 2 31.639184634193718 33.0 31.0 34.0 30.0 34.0 3 31.640927112969756 33.0 31.0 34.0 28.0 34.0 4 35.250042847838756 37.0 35.0 37.0 33.0 37.0 5 35.259397959300266 37.0 35.0 37.0 33.0 37.0 6 35.32839269187262 37.0 35.0 37.0 33.0 37.0 7 35.25768118922748 37.0 35.0 37.0 32.0 37.0 8 35.20410310903918 37.0 35.0 37.0 32.0 37.0 9 36.79597573098413 39.0 37.0 39.0 33.0 39.0 10-11 36.590393514551124 39.0 37.0 39.0 32.0 39.0 12-13 36.50674710634262 39.0 37.0 39.0 32.0 39.0 14-15 37.58131605708475 40.0 37.0 41.0 32.0 41.0 16-17 37.52372113483929 40.0 37.0 41.0 32.0 41.0 18-19 37.51623904523589 40.0 37.0 41.0 32.0 41.0 20-21 37.447823044138985 40.0 37.0 41.0 32.0 41.0 22-23 37.33265690878552 40.0 37.0 41.0 31.5 41.0 24-25 37.16602366343308 39.5 36.5 41.0 31.0 41.0 26-27 37.24939441721226 40.0 37.0 41.0 31.0 41.0 28-29 37.19358910636548 40.0 37.0 41.0 31.0 41.0 30-31 37.01230675624721 39.0 36.0 41.0 31.0 41.0 32-33 36.78039911333539 39.0 35.5 41.0 30.0 41.0 34-35 36.58539545698648 39.0 35.0 41.0 30.0 41.0 36-37 36.39393503125036 39.0 35.0 41.0 30.0 41.0 38-39 36.217543904752105 39.0 35.0 41.0 30.0 41.0 40-41 36.022895599812614 39.0 35.0 40.5 29.5 41.0 42-43 35.7679549583519 39.0 35.0 40.0 28.0 41.0 44-45 35.46063026314286 38.0 35.0 40.0 27.0 41.0 46-47 35.039966178772616 38.0 34.5 40.0 25.0 41.0 48-49 34.66303059906991 38.0 34.0 40.0 24.0 41.0 50-51 33.97939875912659 37.5 33.5 39.5 23.0 40.5 52-53 34.20490722014648 38.0 33.5 39.5 23.0 40.5 54-55 34.57649453261577 38.0 34.0 40.0 23.0 41.0 56-57 34.46325797826758 38.0 34.0 40.0 23.0 41.0 58-59 34.215714016385014 38.0 34.0 40.0 22.5 41.0 60-61 33.9283461305545 38.0 34.0 40.0 22.0 41.0 62-63 33.603379837521 37.0 33.0 40.0 20.5 41.0 64-65 33.28831710828506 37.0 33.0 40.0 20.0 41.0 66-67 32.90861127298072 36.0 32.5 39.0 18.5 41.0 68-69 32.527294073286946 36.0 32.0 39.0 17.5 41.0 70-71 32.11200824963722 35.0 31.5 39.0 13.5 40.0 72-73 31.72346490476354 35.0 31.0 38.0 11.5 40.0 74-75 31.297124624367278 35.0 31.0 37.0 8.0 39.5 76-77 30.874636364675098 35.0 31.0 37.0 7.0 39.0 78-79 30.428648064991602 35.0 30.0 36.0 3.5 39.0 80-81 29.988784435379745 34.5 30.0 36.0 2.0 37.5 82-83 29.578799746340792 34.0 29.5 35.0 2.0 37.0 84-85 29.21804065402941 34.0 29.0 35.0 2.0 37.0 86-87 28.88350529599287 34.0 29.0 35.0 2.0 36.0 88-89 28.528140746580743 34.0 29.0 35.0 2.0 36.0 90-91 28.164043236325824 34.0 28.5 35.0 2.0 35.5 92-93 27.779836378386406 34.0 27.0 35.0 2.0 35.0 94-95 27.345415852557732 34.0 26.5 35.0 2.0 35.0 96-97 26.857273277802534 33.5 25.5 35.0 2.0 35.0 98-99 26.235909059747026 33.0 24.5 35.0 2.0 35.0 100-101 24.0231666838058 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3946613047670091 1101 2 0.37716436098967066 1101 3 0.38111279430415834 1101 4 0.3157013228591268 1101 5 0.27202036462976764 1101 6 0.2782844571856913 1101 7 0.2490312858438557 1101 8 0.2767567924385972 1101 9 0.26594021519901645 1101 10-11 0.4618551314445085 1101 12-13 0.34495632555502453 1101 14-15 0.7476067221160392 1101 16-17 0.6908223962322495 1101 18-19 0.7160640184598961 1101 20-21 0.6548876895888398 1101 22-23 0.7684272527906302 1101 24-25 0.7993226650509442 1101 26-27 0.8654156850129766 1101 28-29 0.8199100241665889 1101 30-31 0.8871640977003779 1101 32-33 0.9021220562835808 1101 34-35 0.9410042317994609 1101 36-37 0.9659285601409664 1101 38-39 1.075613875611289 1101 40-41 1.0606452516598566 1101 42-43 0.8815279297060741 1101 44-45 1.0896681683120377 1101 46-47 1.0618323470779742 1101 48-49 1.1866214427762571 1101 50-51 1.1400013248757688 1101 52-53 1.1422149316348893 1101 54-55 1.2578700919588854 1101 56-57 1.2960965462801113 1101 58-59 1.2141886235495676 1101 60-61 1.336567308995086 1101 62-63 1.1713853481641365 1101 64-65 1.3744977650942758 1101 66-67 1.138835002369369 1101 68-69 1.0756633178331896 1101 70-71 1.205866151462068 1101 72-73 1.3171407398358888 1101 74-75 1.0907064418993464 1101 76-77 1.0187319990290362 1101 78-79 0.9169821380777847 1101 80-81 0.8978144998313269 1101 82-83 0.9950490540502663 1101 84-85 1.032336117558259 1101 86-87 0.9977514591456149 1101 88-89 1.0426529267238642 1101 90-91 1.1866164422731167 1101 92-93 1.3746316301904997 1101 94-95 1.492015112934748 1101 96-97 1.262530262739972 1101 98-99 1.2514392741367573 1101 100-101 1.3298744213579177 1102 1 0.36884076209140915 1102 2 0.3003373567183445 1102 3 0.3969045589611824 1102 4 0.4807625626672305 1102 5 0.2845015035686913 1102 6 0.172486555473931 1102 7 0.39048070743798746 1102 8 0.42779017094106564 1102 9 0.3737148945417488 1102 10-11 0.5534832043939417 1102 12-13 0.594189501334732 1102 14-15 0.46023059756613094 1102 16-17 0.6680060879705962 1102 18-19 0.6626808709044951 1102 20-21 0.6479252855860693 1102 22-23 0.5535292832482668 1102 24-25 0.8100031398344214 1102 26-27 0.6776213486377856 1102 28-29 0.6759733265788057 1102 30-31 0.7373562844145027 1102 32-33 0.7681392308081954 1102 34-35 0.5371851072603206 1102 36-37 0.352850173494204 1102 38-39 0.49699620489273144 1102 40-41 0.3967152909893059 1102 42-43 0.5282204571113311 1102 44-45 0.5064718548515827 1102 46-47 0.7143415411242628 1102 48-49 0.8586924603429935 1102 50-51 0.8679181562482654 1102 52-53 0.8278611450413607 1102 54-55 0.867362069951632 1102 56-57 1.1058393898436591 1102 58-59 0.9740553617895742 1102 60-61 0.9287163394481013 1102 62-63 0.9601477703165955 1102 64-65 0.8589602498477689 1102 66-67 0.7242931050504424 1102 68-69 0.690681259874161 1102 70-71 0.7983340319017813 1102 72-73 0.7132396340893585 1102 74-75 0.659660789287404 1102 76-77 0.8312006792854056 1102 78-79 0.81065543450676 1102 80-81 0.921800539954436 1102 82-83 0.7388576271619698 1102 84-85 0.8113438527082018 1102 86-87 0.795168051342177 1102 88-89 0.8463165484475006 1102 90-91 0.7520918948232271 1102 92-93 0.8241536628322486 1102 94-95 0.9252107342461677 1102 96-97 0.8992186218699558 1102 98-99 0.9303405721469069 1102 100-101 1.0853518580685808 1103 1 0.36581843743558906 1103 2 0.44824957562984835 1103 3 0.45588212582534027 1103 4 0.3336482350937118 1103 5 0.39242519883703153 1103 6 0.31006116142616236 1103 7 0.2193484279021476 1103 8 0.4593741601191468 1103 9 0.40953375423884353 1103 10-11 0.6355606443127542 1103 12-13 0.43575635007592695 1103 14-15 0.5777263213487132 1103 16-17 0.6886155145291752 1103 18-19 0.5576492353428435 1103 20-21 0.6893279026815193 1103 22-23 0.798679025697119 1103 24-25 0.7948030430915765 1103 26-27 0.8317167808284296 1103 28-29 0.7548430476991825 1103 30-31 0.8118644267889081 1103 32-33 0.7672684266659147 1103 34-35 0.790255521763676 1103 36-37 0.6722699061294719 1103 38-39 0.9024520092113661 1103 40-41 0.8285717547242228 1103 42-43 0.8131198300315745 1103 44-45 0.8374721266444354 1103 46-47 0.8253589918851532 1103 48-49 0.8673179561722861 1103 50-51 0.8102073752602479 1103 52-53 0.7798947453956089 1103 54-55 0.8799084073725183 1103 56-57 1.0002099285655461 1103 58-59 0.9805623849820719 1103 60-61 0.9688797352641529 1103 62-63 1.0408336426901386 1103 64-65 0.96026621894638 1103 66-67 0.9337220940551347 1103 68-69 0.9410762783291133 1103 70-71 0.763878582366182 1103 72-73 0.8011982622971878 1103 74-75 0.7624586551355499 1103 76-77 0.8936613145219106 1103 78-79 0.9893814640518563 1103 80-81 1.0222050281821282 1103 82-83 1.0717579295722715 1103 84-85 0.9366317619572477 1103 86-87 1.0149367280607322 1103 88-89 1.0141896849103347 1103 90-91 1.0081130549489323 1103 92-93 1.001166093279803 1103 94-95 1.026810281119765 1103 96-97 1.0190016143952114 1103 98-99 1.0330192878026772 1103 100-101 1.0809994675215187 1104 1 0.45470732632447763 1104 2 0.3986991193764986 1104 3 0.4576735912037577 1104 4 0.47737475146515607 1104 5 0.3999466695831444 1104 6 0.4935018484900411 1104 7 0.4147724751377879 1104 8 0.4327168869038971 1104 9 0.4576215159135586 1104 10-11 0.7082793775387302 1104 12-13 0.700960322163958 1104 14-15 0.7139716246229995 1104 16-17 0.80150802660647 1104 18-19 0.8702263958366743 1104 20-21 0.8203631952204304 1104 22-23 0.8139568034749018 1104 24-25 0.7609362959096089 1104 26-27 0.6050964104580601 1104 28-29 0.6745424572536649 1104 30-31 0.8460096227094596 1104 32-33 0.8760714325703702 1104 34-35 0.8266618556337804 1104 36-37 0.8584447585180897 1104 38-39 0.8001740972414311 1104 40-41 0.708811958696316 1104 42-43 0.6725166362580879 1104 44-45 0.8275050037244114 1104 46-47 0.851140661235668 1104 48-49 0.9710619175249491 1104 50-51 0.8764681106333114 1104 52-53 0.806676146361788 1104 54-55 0.9216455249248554 1104 56-57 0.9485929129992527 1104 58-59 0.8001376667856093 1104 60-61 0.9702183020918511 1104 62-63 1.0353015707631457 1104 64-65 1.1845952581304857 1104 66-67 1.0839066082794986 1104 68-69 0.8912675021616323 1104 70-71 0.797617846993127 1104 72-73 0.8837365070636913 1104 74-75 0.881617941233241 1104 76-77 0.9161720498949677 1104 78-79 0.9177262037190594 1104 80-81 0.9105605032223885 1104 82-83 0.8723819231310479 1104 84-85 0.9318176181032527 1104 86-87 1.015939814480486 1104 88-89 0.9739051975243989 1104 90-91 1.0027822712666996 1104 92-93 0.9633306128718573 1104 94-95 0.9985292827300718 1104 96-97 1.0717156508954737 1104 98-99 1.1883299426604346 1104 100-101 1.224172441219853 1105 1 0.0449709710978361 1105 2 0.26029717673079844 1105 3 0.23583039046370402 1105 4 0.22622997810715617 1105 5 0.12435693785326407 1105 6 0.18360360612186355 1105 7 0.1598696074227135 1105 8 0.16679413019714673 1105 9 0.24181439287933415 1105 10-11 0.2948427447912749 1105 12-13 0.29852045167367436 1105 14-15 0.2807269437719313 1105 16-17 -0.022017644068824893 1105 18-19 0.06684411091530507 1105 20-21 0.14901143758860513 1105 22-23 0.2240516230863676 1105 24-25 0.28601145779675363 1105 26-27 0.25532189611948297 1105 28-29 0.2856548531833525 1105 30-31 -0.1380907473182944 1105 32-33 0.10741972119201648 1105 34-35 -0.009358033357891316 1105 36-37 -0.018237711417150138 1105 38-39 -0.14925351607957538 1105 40-41 0.036008998474294174 1105 42-43 0.046724545601293244 1105 44-45 -0.04386988064190689 1105 46-47 0.04112100351133563 1105 48-49 0.246420381909779 1105 50-51 0.06087490989325772 1105 52-53 0.02406166902598983 1105 54-55 0.16389228718202986 1105 56-57 0.28756146064032606 1105 58-59 0.020807185749532664 1105 60-61 0.07333462956641057 1105 62-63 0.022969239588299217 1105 64-65 -0.10077708229503912 1105 66-67 -0.06643451105444598 1105 68-69 -0.04430719719081111 1105 70-71 -0.10039903552306129 1105 72-73 -0.0032720498280731647 1105 74-75 0.03324259433222565 1105 76-77 0.13601478810033285 1105 78-79 0.1557985903980601 1105 80-81 0.14026101201054786 1105 82-83 0.25270107206721804 1105 84-85 0.14975818239557626 1105 86-87 0.10959730754246522 1105 88-89 -0.03296344262361117 1105 90-91 -0.13269992114772933 1105 92-93 -0.09629010868225762 1105 94-95 -0.08057571264458119 1105 96-97 -0.17190715206474394 1105 98-99 -0.14159027010552094 1105 100-101 0.12172678904594036 1106 1 0.16553695088227727 1106 2 0.18403091214153022 1106 3 0.17441585983322483 1106 4 0.021536282904165205 1106 5 0.1787253412709191 1106 6 0.05676214747440156 1106 7 0.019705146597338796 1106 8 0.21669640305133697 1106 9 0.07085471230749363 1106 10-11 0.230957113473913 1106 12-13 0.2537076689630027 1106 14-15 0.3460582387962319 1106 16-17 0.41348523190146125 1106 18-19 0.1361277709365183 1106 20-21 0.20603662327018668 1106 22-23 0.019056369833457154 1106 24-25 0.23832061064201326 1106 26-27 0.032885837594882616 1106 28-29 0.18439068274721393 1106 30-31 0.4121898692003896 1106 32-33 0.2676496592050839 1106 34-35 0.15324807040041577 1106 36-37 0.11014294977680095 1106 38-39 0.2986641976636548 1106 40-41 0.2210295211749127 1106 42-43 0.26993404467986437 1106 44-45 0.26310853561988523 1106 46-47 0.44425874294284284 1106 48-49 0.4695698485987805 1106 50-51 0.47780683595568973 1106 52-53 0.34935345436134213 1106 54-55 0.4017971567860954 1106 56-57 0.33867764425567515 1106 58-59 0.4481470528406106 1106 60-61 0.3944087905090612 1106 62-63 0.4596518218187171 1106 64-65 0.37652439198875953 1106 66-67 0.5637401413161385 1106 68-69 0.49970282682213707 1106 70-71 0.42258289891599077 1106 72-73 0.5317687163527474 1106 74-75 0.3964280804549709 1106 76-77 0.54809176916919 1106 78-79 0.4719905823565149 1106 80-81 0.6013991144285953 1106 82-83 0.4424934716874809 1106 84-85 0.5215464495536679 1106 86-87 0.44609001988959207 1106 88-89 0.5010134937699782 1106 90-91 0.5743908813317482 1106 92-93 0.5299559124268072 1106 94-95 0.3215393820111423 1106 96-97 0.427874357959265 1106 98-99 0.5012400476473111 1106 100-101 0.3996139281535527 1107 1 0.2396717450899537 1107 2 0.25798175038013227 1107 3 0.25445259715999313 1107 4 0.27858579614805024 1107 5 0.3223960304594016 1107 6 0.20727368242940258 1107 7 0.24317555810118563 1107 8 0.3060122780743981 1107 9 0.4835425568040961 1107 10-11 0.5537458374847475 1107 12-13 0.4132168862882608 1107 14-15 0.35790441066712475 1107 16-17 0.3847894474019711 1107 18-19 0.33900400196690583 1107 20-21 0.4329863074072833 1107 22-23 0.39430095989848013 1107 24-25 0.49357998721172436 1107 26-27 0.3929389774377796 1107 28-29 0.3901357380888797 1107 30-31 0.4114553920002848 1107 32-33 0.4478183707995598 1107 34-35 0.4022429786177 1107 36-37 0.2860002775133452 1107 38-39 0.07195826191896515 1107 40-41 0.47226395191468384 1107 42-43 0.35355415271023105 1107 44-45 0.47887568991799867 1107 46-47 0.4204425049905396 1107 48-49 0.3334779837350439 1107 50-51 0.27300660863674153 1107 52-53 0.3803054601682092 1107 54-55 0.4827820888260774 1107 56-57 0.472065652642776 1107 58-59 0.36260685388267433 1107 60-61 0.43218723264709524 1107 62-63 0.34660683879356924 1107 64-65 0.2860447193926774 1107 66-67 0.22550611861925773 1107 68-69 0.3594567352131435 1107 70-71 0.10760220351932759 1107 72-73 0.2470600899490094 1107 74-75 0.5519992125652529 1107 76-77 0.4764551939822361 1107 78-79 0.47291211288094814 1107 80-81 0.2621860581334907 1107 82-83 0.33380444750251925 1107 84-85 0.43470859340831325 1107 86-87 0.2188489150225692 1107 88-89 0.3685880895314462 1107 90-91 0.2709132247099397 1107 92-93 0.3057500810495206 1107 94-95 0.024873617617526378 1107 96-97 -0.04302504667518292 1107 98-99 0.015789537324362612 1107 100-101 0.0464959014112587 1108 1 0.2151939928400992 1108 2 0.12805742420011512 1108 3 0.2314991230704493 1108 4 0.08311964803719718 1108 5 0.167239700373635 1108 6 0.049832138269586324 1108 7 0.1564195059735951 1108 8 0.2033025066786962 1108 9 0.1578863164210702 1108 10-11 0.3029952621706755 1108 12-13 0.38386978587920595 1108 14-15 0.4168246730857419 1108 16-17 0.28450004577406673 1108 18-19 0.15483638858651716 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-2.3247881834056408 2316 74-75 -2.554716519847478 2316 76-77 -2.7707280844749356 2316 78-79 -2.680647560557741 2316 80-81 -2.9338301532705557 2316 82-83 -2.6796324256548267 2316 84-85 -2.9593577586123665 2316 86-87 -3.5735763339022775 2316 88-89 -3.4519010218824526 2316 90-91 -2.9630871739775664 2316 92-93 -2.9568068763305604 2316 94-95 -3.321613005397385 2316 96-97 -3.377121209647761 2316 98-99 -3.0956794392380225 2316 100-101 -3.1528317098503074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4304.0 3 1628.0 4 1660.0 5 1792.0 6 1994.0 7 2399.0 8 2971.0 9 3704.0 10 4633.0 11 5914.0 12 7691.0 13 9175.0 14 10587.0 15 12989.0 16 16199.0 17 20842.0 18 18092.0 19 11679.0 20 11069.0 21 11764.0 22 12764.0 23 14195.0 24 15713.0 25 18292.0 26 21222.0 27 24810.0 28 29856.0 29 35071.0 30 42770.0 31 52755.0 32 65603.0 33 84723.0 34 112378.0 35 158232.0 36 228764.0 37 328933.0 38 291226.0 39 51955.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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22.21516912523147 18-19 21.311379365827406 27.17303610464354 30.665069293948495 20.850515235580563 20-21 22.597004687321366 26.09437521435921 30.540156625128617 20.76846347319081 22-23 22.10754186312516 26.350616727991337 30.378157539591488 21.16368386929201 24-25 21.701937029609848 26.427330462411213 30.641203398822853 21.229529109156086 26-27 21.633464780974403 26.61030502652237 30.451884478064873 21.304345714438355 28-29 21.345097407309634 26.75304015715907 30.595477676943943 21.306384758587352 30-31 21.631144381995668 26.45031513094197 30.492150509771903 21.426389977290462 32-33 21.407223262718013 26.60312849596111 30.38430861244839 21.605339628872482 34-35 21.772949210240856 26.61877840948859 30.292731827569135 21.315540552701414 36-37 21.87950553040243 26.39929794070033 30.199805231107373 21.52139129778987 38-39 21.626686603133937 26.71665638704476 30.242000106843314 21.414656902977985 40-41 21.815420636838738 26.6295345949896 30.083348616960315 21.47169615121135 42-43 21.597520644394784 26.69186624986038 29.865419713893697 21.84519339185114 44-45 21.83107060639292 26.751462628941443 29.570399435315682 21.847067329349958 46-47 22.07947429251791 27.038976145600223 29.121187069616134 21.76036249226573 48-49 22.05203544747394 27.234013258381324 28.710762815402163 22.00318847874257 50-51 22.011404496837496 27.420898836153984 28.609712362260893 21.957984304747626 52-53 22.19932113757734 27.519266906285335 28.266061278160475 22.01535067797685 54-55 21.96635973452541 27.397093172073973 28.349485551563685 22.28706154183693 56-57 22.117709916684763 27.73991702762317 28.069520794523367 22.0728522611687 58-59 22.3947544415524 27.33140612996938 28.126570352207413 22.1472690762708 60-61 22.226009491347217 27.25830921449245 28.65438296243175 21.861298331728584 62-63 22.12203552724768 27.316360606963574 28.679865940736114 21.881737925052633 64-65 22.029401446474832 27.44059774218283 28.520825786516408 22.009175024825932 66-67 22.03801340177262 27.642941670233196 28.121523347391843 22.19752158060234 68-69 22.047758184900957 27.474699968398177 28.335178201713358 22.142363644987512 70-71 22.035953977537336 27.412365036871112 28.28752896403745 22.2641520215541 72-73 22.04745781307097 27.462824389292006 28.255978368475894 22.23373942916113 74-75 21.902856633478702 27.868261837497826 27.989659617652705 22.239221911370763 76-77 21.9842750879551 27.652889581881464 28.071885312205254 22.290950017958185 78-79 21.972985121499512 27.64257193713693 27.998224878617645 22.38621806274591 80-81 21.861501239989288 27.841567115581174 27.954436287067164 22.34249535736237 82-83 21.912207270894363 27.732643131374207 28.053475031231677 22.301674566499756 84-85 21.977020400722978 27.683034216825607 27.841463965160884 22.498481417290527 86-87 22.05912984444793 27.61355678246283 27.923455158310755 22.403858214778488 88-89 22.05915672030914 27.498517867729515 27.78031585488675 22.66200955707459 90-91 21.95052824235207 27.477859191371213 27.960302825806593 22.611309740470123 92-93 22.00843747303283 27.434531575659797 27.911353956108453 22.645676995198915 94-95 21.910824895290553 27.42114612235755 27.869527415317425 22.798501567034474 96-97 21.964172073946497 27.5727978890391 27.81423589827999 22.648794138734413 98-99 21.820274436469507 27.644854251890393 27.606670593638704 22.928200718001392 100-101 21.912647933476098 27.461032227231307 27.761615443277353 22.86470439601524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 921.0 1 670.0 2 503.0 3 571.0 4 510.0 5 542.5 6 902.5 7 1815.5 8 3103.0 9 3654.0 10 3106.0 11 2686.5 12 2770.5 13 2860.0 14 2987.0 15 3113.5 16 3185.0 17 3336.0 18 3566.5 19 3811.5 20 4126.0 21 4687.5 22 5575.0 23 6638.0 24 7838.5 25 9568.5 26 11689.0 27 14126.0 28 16426.5 29 18864.0 30 22111.0 31 25205.0 32 28222.0 33 31385.0 34 35309.5 35 38525.5 36 41969.5 37 46318.5 38 48533.5 39 49916.5 40 51691.0 41 53893.5 42 55800.0 43 58225.5 44 61248.5 45 63278.5 46 63420.0 47 64649.0 48 69112.5 49 69292.5 50 64903.0 51 61823.0 52 59772.5 53 57934.5 54 55261.0 55 50444.5 56 45147.0 57 40947.0 58 37527.5 59 33533.0 60 28529.5 61 23429.0 62 18753.5 63 14170.5 64 10246.0 65 7690.0 66 5507.5 67 3987.0 68 2914.5 69 1914.5 70 1275.5 71 825.0 72 573.5 73 428.5 74 331.0 75 265.5 76 173.5 77 82.5 78 42.0 79 32.0 80 26.5 81 14.5 82 7.5 83 7.0 84 10.0 85 8.0 86 5.5 87 8.0 88 8.0 89 5.5 90 5.0 91 4.0 92 2.0 93 1.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04056262068807916 2 0.04119105565648602 3 0.025137398736274408 4 0.04290496920668655 5 0.06364332316411293 6 0.06387184497080634 7 0.06278636638901267 8 0.06478593219757996 9 0.063814714519133 10-11 0.07092745575246517 12-13 0.0712416732366686 14-15 0.06584284555353695 16-17 0.05435962476719341 18-19 0.04801814463145146 20-21 0.055987842639883906 22-23 0.030879009129446175 24-25 0.01733909208286201 26-27 0.014482569499194463 28-29 0.017481918212045387 30-31 0.013397090917400793 32-33 0.008341045944309235 34-35 0.011111872850466756 36-37 0.005741610393171768 38-39 0.008769524331859367 40-41 0.008055393685942481 42-43 0.004941784069744855 44-45 0.001228304710977045 46-47 0.003027913938687599 48-49 8.855220009369395E-4 50-51 0.006027262651538523 52-53 0.005655914715661742 54-55 0.008912350461042745 56-57 0.022852180669340375 58-59 0.010026394268673088 60-61 0.02085261486077309 62-63 0.014768221757561215 64-65 0.011083307624630081 66-67 0.01788183137375884 68-69 0.027479747254881795 70-71 0.02085261486077309 72-73 0.01951004924644934 74-75 0.019881397182326125 76-77 0.027508312480718472 78-79 0.036592054296781265 80-81 0.0323644008729533 82-83 0.03165027022703642 84-85 0.022452267507626914 86-87 0.025794398930517944 88-89 0.020995440989956468 90-91 0.01891017950387916 92-93 0.03239296609878998 94-95 0.023909094025297364 96-97 0.03744901107188153 98-99 0.05373118979878654 100-101 0.06364332316411293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1750380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.1621158203279 #Duplication Level Percentage of deduplicated Percentage of total 1 75.94105202702461 42.65010159459303 2 13.478647834444562 15.139787615589745 3 4.59186137294808 7.736659507751999 4 2.047156391140588 4.598905373662487 5 1.0729632991256097 3.0129944538226807 6 0.6570966068030405 2.2142361441850094 7 0.4293504148292249 1.6879259407601332 8 0.30102391334920553 1.3524911908965143 9 0.2210802417300442 1.1174700727465947 >10 1.142764277328444 11.675686020430204 >50 0.07989276774940074 3.0819769861146895 >100 0.03446045235404525 3.27931162995503 >500 0.001732954613179575 0.6065724609395304 >1k 6.116310399457322E-4 0.46813997292142906 >5k 2.0387701331524408E-4 0.741569899627158 >10k+ 1.0193850665762204E-4 0.6361711360037813 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11112 0.6348335789942755 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7695 0.4396188256264354 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5258 0.30039191489847916 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1914 0.10934768450279368 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.713045167335093E-5 2 0.0 0.0 0.0 0.0 5.713045167335093E-5 3 0.0 0.0 0.0 0.0 5.713045167335093E-5 4 0.0 0.0 0.0 0.0 5.713045167335093E-5 5 0.0 0.0 0.0 0.0 5.713045167335093E-5 6 0.0 0.0 0.0 0.0 5.713045167335093E-5 7 0.0 0.0 0.0 0.0 5.713045167335093E-5 8 0.0 0.0 0.0 0.0 5.713045167335093E-5 9 0.0 0.0 0.0 0.0 5.713045167335093E-5 10-11 0.0 0.0 0.0 0.0 5.713045167335093E-5 12-13 0.0 0.0 0.0 0.0 1.9995658085672827E-4 14-15 0.0 0.0 0.0 0.0 2.570870325300792E-4 16-17 0.0 0.0 0.0 0.0 2.8565225836675465E-4 18-19 0.0 0.0 0.0 0.0 3.4278271004010557E-4 20-21 0.0 0.0 0.0 8.56956775100264E-5 3.999131617134565E-4 22-23 0.0 0.0 0.0 3.142174842034301E-4 3.999131617134565E-4 24-25 1.1426090334670186E-4 0.0 0.0 6.284349684068602E-4 3.999131617134565E-4 26-27 1.1426090334670186E-4 0.0 0.0 0.0012568699368137204 3.999131617134565E-4 28-29 1.1426090334670186E-4 0.0 0.0 0.0019995658085672826 3.999131617134565E-4 30-31 1.7139135502005278E-4 0.0 0.0 0.004370479553011346 4.28478387550132E-4 32-33 2.2852180669340373E-4 0.0 0.0 0.007655480524229024 4.5704361338680746E-4 34-35 3.142174842034301E-4 0.0 0.0 0.011054742398793404 4.5704361338680746E-4 36-37 3.4278271004010557E-4 0.0 0.0 0.014025525885807653 5.141740650601584E-4 38-39 3.4278271004010557E-4 0.0 0.0 0.01636787440441504 5.141740650601584E-4 40-41 3.4278271004010557E-4 0.0 0.0 0.019367223117265964 5.713045167335093E-4 42-43 3.4278271004010557E-4 0.0 0.0 0.023109267701870453 5.713045167335093E-4 44-45 4.5704361338680746E-4 0.0 0.0 0.026622790479781534 5.713045167335093E-4 46-47 5.141740650601584E-4 0.0 0.0 0.0320216181629132 5.713045167335093E-4 48-49 5.141740650601584E-4 0.0 0.0 0.037763228556084966 5.713045167335093E-4 50-51 5.141740650601584E-4 0.0 0.0 0.04276214307750317 5.713045167335093E-4 52-53 5.141740650601584E-4 0.0 0.0 0.05470240747723351 5.713045167335093E-4 54-55 5.141740650601584E-4 0.0 0.0 0.07872576240587759 5.713045167335093E-4 56-57 5.141740650601584E-4 0.0 0.0 0.12323038425941796 5.713045167335093E-4 58-59 5.141740650601584E-4 0.0 0.0 0.1729053119893966 5.713045167335093E-4 60-61 5.141740650601584E-4 0.0 0.0 0.21838115152138393 5.713045167335093E-4 62-63 5.141740650601584E-4 0.0 0.0 0.27579725545310163 5.713045167335093E-4 64-65 5.713045167335093E-4 0.0 0.0 0.3421828402975354 5.713045167335093E-4 66-67 6.284349684068602E-4 0.0 0.0 0.44116134782161587 5.713045167335093E-4 68-69 6.284349684068602E-4 0.0 0.0 0.5663341674379277 5.713045167335093E-4 70-71 6.570001942435357E-4 0.0 0.0 0.6734823295512975 5.713045167335093E-4 72-73 7.426958717535621E-4 0.0 0.0 0.7794307521795267 6.570001942435357E-4 74-75 7.426958717535621E-4 0.0 0.0 0.8911493504267645 6.855654200802111E-4 76-77 7.426958717535621E-4 0.0 0.0 1.024548955084039 6.855654200802111E-4 78-79 7.426958717535621E-4 0.0 0.0 1.1347250311360964 6.855654200802111E-4 80-81 8.569567751002639E-4 0.0 0.0 1.2561843713936403 6.855654200802111E-4 82-83 8.569567751002639E-4 0.0 0.0 1.3996960659970978 7.426958717535621E-4 84-85 8.569567751002639E-4 0.0 0.0 1.6114215198985362 7.426958717535621E-4 86-87 8.569567751002639E-4 0.0 0.0 1.8623384636478937 7.712610975902375E-4 88-89 8.569567751002639E-4 0.0 0.0 2.1582456380900146 7.99826323426913E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11495 0.0 38.362644 1 GGTATCA 6775 0.0 36.12164 1 ATCAACG 14315 0.0 30.572107 3 TCAACGC 14365 0.0 30.498775 4 TATCAAC 14395 0.0 30.403072 2 CAACGCA 14505 0.0 30.178547 5 AACGCAG 14850 0.0 29.475744 6 CGCGGAA 120 0.0 27.707674 82-83 ACGCAGA 16400 0.0 26.371155 7 GTACATG 10375 0.0 26.061157 1 CGCAGAG 16455 0.0 26.05344 8 TACATGG 10440 0.0 25.534765 2 ACATGGG 10605 0.0 24.19581 3 GCAGAGT 17170 0.0 24.110395 9 GAGTACT 8395 0.0 23.06721 12-13 AGAGTAC 13860 0.0 21.825941 10-11 AGTACTT 8890 0.0 21.328447 12-13 GTACTTT 9100 0.0 21.252504 14-15 CCGTATG 115 0.0037110094 20.664675 9 CATGGGG 4110 0.0 20.001446 4 >>END_MODULE