FastQCFastQC Report
Thu 26 May 2016
SRR937126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750380
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114150.6521441058513009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83180.47521109701893305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60610.34626766759218No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20740.11848855677052983No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18740.10706246643585964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121000.036.9950261
GGTATCA72550.036.679921
ATCAACG152450.029.2889983
TATCAAC153500.029.1856542
TCAACGC154450.028.9064254
CAACGCA156150.028.5917195
AACGCAG159950.027.910866
ACGCAGA180450.024.661097
CGCAGAG181250.024.4736358
GTACATG96550.023.7231641
TACATGG98350.022.9931622
AGTACTT102650.022.97284112-13
GAGTACT99050.022.89672312-13
GCAGAGT189650.022.8887989
AGAGTAC154900.021.87621710-11
ACATGGG98750.021.5979883
GTACTTT106550.021.1305314-15
TACTTTT116050.020.13749314-15
CGATCGT2150.018.7846224-25
ACTTTTT122900.018.59023316-17
Produced by FastQC (version 0.11.5)