##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937126_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1750380 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03703138747015 31.0 31.0 34.0 30.0 34.0 2 32.09856831088106 33.0 31.0 34.0 30.0 34.0 3 32.2326631931352 33.0 31.0 34.0 30.0 34.0 4 35.95187387881489 37.0 35.0 37.0 35.0 37.0 5 35.85271369645449 37.0 35.0 37.0 35.0 37.0 6 35.882800306219224 37.0 35.0 37.0 35.0 37.0 7 35.828596647585094 37.0 35.0 37.0 35.0 37.0 8 35.810985043247754 37.0 35.0 37.0 33.0 37.0 9 37.46804979490168 39.0 37.0 39.0 34.0 39.0 10-11 37.35786057884573 39.0 37.0 39.0 34.0 39.0 12-13 37.27984037751803 39.0 37.0 39.0 34.0 39.0 14-15 38.432698042710726 40.0 38.0 41.0 34.0 41.0 16-17 38.360944194974806 40.0 38.0 41.0 33.0 41.0 18-19 38.41862938333391 40.0 38.0 41.0 33.5 41.0 20-21 38.5190952821673 40.0 38.0 41.0 34.0 41.0 22-23 38.44774791759504 40.0 38.0 41.0 34.0 41.0 24-25 38.36098989933615 40.0 38.0 41.0 34.0 41.0 26-27 38.17541133925205 40.0 38.0 41.0 33.5 41.0 28-29 38.01043830482524 40.0 37.0 41.0 33.0 41.0 30-31 37.91923982221003 40.0 37.0 41.0 33.0 41.0 32-33 37.806435459728746 40.0 37.0 41.0 33.0 41.0 34-35 37.670443275174534 40.0 37.0 41.0 33.0 41.0 36-37 37.481477450610726 40.0 36.0 41.0 33.0 41.0 38-39 37.33418943315166 40.0 36.0 41.0 32.0 41.0 40-41 37.085978758898065 39.5 36.0 41.0 31.0 41.0 42-43 36.829143671659864 39.0 36.0 41.0 31.0 41.0 44-45 36.54206486591483 39.0 35.0 41.0 30.5 41.0 46-47 36.12914081513728 39.0 35.0 41.0 29.5 41.0 48-49 35.72315725728127 39.0 35.0 40.5 28.0 41.0 50-51 35.46746563603332 39.0 35.0 40.0 27.0 41.0 52-53 35.24008786663467 38.5 35.0 40.0 27.0 41.0 54-55 34.96268724505536 38.0 34.0 40.0 26.0 41.0 56-57 34.709140586615476 38.0 34.0 40.0 26.0 41.0 58-59 34.36207195009084 38.0 34.0 40.0 25.0 41.0 60-61 34.021101703630066 37.5 33.0 40.0 24.0 41.0 62-63 33.687195066214194 37.0 33.0 40.0 23.0 41.0 64-65 33.27271163975823 36.0 32.5 39.0 22.0 40.5 66-67 32.837564128932 36.0 32.0 39.0 22.0 40.0 68-69 32.39804099681212 35.0 31.5 38.5 20.0 40.0 70-71 32.00550509032324 35.0 31.0 38.0 20.0 40.0 72-73 31.612799506392896 35.0 31.0 37.0 18.0 39.5 74-75 31.134601058055964 35.0 30.5 37.0 16.5 39.0 76-77 29.671646442486775 33.5 28.5 35.0 13.0 37.5 78-79 30.378992561615192 34.0 30.0 36.0 12.0 38.0 80-81 30.111267838983537 34.0 30.0 35.5 7.5 37.0 82-83 29.88622184896994 34.0 30.0 35.0 7.0 37.0 84-85 29.54114649390418 34.0 29.5 35.0 4.0 36.5 86-87 29.160815365806283 34.0 29.0 35.0 2.0 36.0 88-89 28.76967087146791 34.0 29.0 35.0 2.0 36.0 90-91 28.303187022246597 34.0 29.0 35.0 2.0 35.0 92-93 27.846782698614014 33.0 27.0 35.0 2.0 35.0 94-95 27.344108707823445 33.0 26.5 35.0 2.0 35.0 96-97 26.690701733337903 33.0 25.5 34.5 2.0 35.0 98-99 25.879695551823033 32.5 24.5 34.0 2.0 35.0 100-101 22.710219780847588 29.0 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34127999797976116 1101 2 0.3256673582916889 1101 3 0.23759373805246753 1101 4 0.3010204077440619 1101 5 0.3219258172426507 1101 6 0.23246802418856305 1101 7 0.2588275758573886 1101 8 0.2848368296954433 1101 9 0.39973877332824514 1101 10-11 0.41125495639240484 1101 12-13 0.36639079254450735 1101 14-15 0.5073393105419655 1101 16-17 0.3850745766079555 1101 18-19 0.45296936589596015 1101 20-21 0.4874437904745008 1101 22-23 0.4988119426029485 1101 24-25 0.34632193953306967 1101 26-27 0.5069532926163802 1101 28-29 0.44394911548295823 1101 30-31 0.42083944860756617 1101 32-33 0.5593202607731413 1101 34-35 0.5998028784388438 1101 36-37 0.5668295244950414 1101 38-39 0.4502669645480992 1101 40-41 0.5592575937788951 1101 42-43 0.6174474466884661 1101 44-45 0.6562859918707389 1101 46-47 0.5887646773929376 1101 48-49 0.49586667310182975 1101 50-51 0.4812499029166233 1101 52-53 0.4778250662479664 1101 54-55 0.3643256009105542 1101 56-57 0.351504458815846 1101 58-59 0.45275772748818355 1101 60-61 0.346736715892483 1101 62-63 0.32806703854305397 1101 64-65 0.539522625154909 1101 66-67 0.4037860265329343 1101 68-69 0.5006161999127201 1101 70-71 0.6102280190166951 1101 72-73 0.5889467731933138 1101 74-75 0.42359135264255343 1101 76-77 0.45227485597230555 1101 78-79 0.6813794431166542 1101 80-81 1.040266348619717 1101 82-83 0.8258539169138288 1101 84-85 0.7106474308283133 1101 86-87 0.7948305208632611 1101 88-89 0.510029086121822 1101 90-91 0.25400802217975027 1101 92-93 0.46027991766823817 1101 94-95 0.6277789601192687 1101 96-97 0.7304564836115368 1101 98-99 0.9209856489698538 1101 100-101 1.142639153297413 1102 1 0.2523253122077307 1102 2 0.2424076747422248 1102 3 0.16275058671331522 1102 4 0.19165778471457173 1102 5 0.1916138426029903 1102 6 0.23810488589257517 1102 7 0.28890314315042787 1102 8 0.32320987956554603 1102 9 0.38594716555833486 1102 10-11 0.3163405598463882 1102 12-13 0.3443698404242994 1102 14-15 0.4024215147751491 1102 16-17 0.42044985212171326 1102 18-19 0.3652199423022964 1102 20-21 0.2750932369859882 1102 22-23 0.37745960644615906 1102 24-25 0.2530483976219031 1102 26-27 0.3112521533390691 1102 28-29 0.3277292951033246 1102 30-31 0.4666140325875432 1102 32-33 0.3934765849388313 1102 34-35 0.35658109389407144 1102 36-37 0.4528591884643802 1102 38-39 0.47776450741904597 1102 40-41 0.5352941638574436 1102 42-43 0.40924963357177546 1102 44-45 0.4333458658380991 1102 46-47 0.5385218868941379 1102 48-49 0.7764074414487752 1102 50-51 0.7768804429928124 1102 52-53 0.5546971371117237 1102 54-55 0.7983101183057926 1102 56-57 0.9442373554287755 1102 58-59 1.0087275128758435 1102 60-61 0.9607750363390224 1102 62-63 0.7372976918240752 1102 64-65 0.7410831905022377 1102 66-67 0.7000545742043229 1102 68-69 0.7219976063287916 1102 70-71 0.7762661118231193 1102 72-73 0.7269382182312967 1102 74-75 0.7887488780713419 1102 76-77 0.6980169858533003 1102 78-79 0.6190085096844733 1102 80-81 0.6850385469927041 1102 82-83 0.7657432345933124 1102 84-85 0.44034862240132 1102 86-87 0.5294327428154588 1102 88-89 0.6188252236226113 1102 90-91 0.5833923633077056 1102 92-93 0.7514144114535064 1102 94-95 0.869052114426971 1102 96-97 0.9310449640983158 1102 98-99 1.2006626153267952 1102 100-101 1.38444295519513 1103 1 0.21787045545916328 1103 2 0.15667703657438636 1103 3 0.1549050504144418 1103 4 0.15220840087222598 1103 5 0.14762163538124184 1103 6 0.2407264865183194 1103 7 0.17159414728126166 1103 8 0.13486247198623147 1103 9 0.21487535029923066 1103 10-11 0.11084841768301601 1103 12-13 0.21710750578594684 1103 14-15 0.36172683358638835 1103 16-17 0.39595737414449417 1103 18-19 0.38821607762726984 1103 20-21 0.36893379446662067 1103 22-23 0.32522439922411905 1103 24-25 0.2668650776410999 1103 26-27 0.36262278068262077 1103 28-29 0.25835335460966036 1103 30-31 0.33720580891240104 1103 32-33 0.31759599240077563 1103 34-35 0.41299811719623136 1103 36-37 0.3540940527591303 1103 38-39 0.285756343556109 1103 40-41 0.4912540890832844 1103 42-43 0.4529565620369027 1103 44-45 0.6394908147146552 1103 46-47 0.45788373387625114 1103 48-49 0.4077706767381031 1103 50-51 0.4320117705126876 1103 52-53 0.42282780989831537 1103 54-55 0.6327208420602233 1103 56-57 0.6064652328392341 1103 58-59 0.6648674675217023 1103 60-61 0.6693880540014092 1103 62-63 0.5996027501054115 1103 64-65 0.3633880472359863 1103 66-67 0.44172480500298406 1103 68-69 0.5502900269834967 1103 70-71 0.7647254375930785 1103 72-73 0.725197795520792 1103 74-75 0.5518931153070774 1103 76-77 0.6625087465582169 1103 78-79 0.7430791307741167 1103 80-81 1.0014313608726937 1103 82-83 0.8420740488781782 1103 84-85 0.7283353783535667 1103 86-87 0.6639527172189155 1103 88-89 0.6903842505389939 1103 90-91 0.7074776558762608 1103 92-93 0.5618845419526082 1103 94-95 0.8311574627590019 1103 96-97 1.0642618444202476 1103 98-99 1.0440284244973874 1103 100-101 1.0955261163979308 1104 1 0.2183736760710815 1104 2 0.19355975262377 1104 3 0.2065019371562613 1104 4 0.14645155707190582 1104 5 0.04289136162923057 1104 6 0.1503816126589541 1104 7 0.15050584883788787 1104 8 0.1531864118681412 1104 9 0.3142446471322913 1104 10-11 0.2915012625568707 1104 12-13 0.18585079617778888 1104 14-15 0.2365758674028129 1104 16-17 0.3013418346920034 1104 18-19 0.2765380046267367 1104 20-21 0.25147203923312844 1104 22-23 0.2306356982042601 1104 24-25 0.26935769707212387 1104 26-27 0.3120088515360777 1104 28-29 0.14747037017594522 1104 30-31 0.2655371293632882 1104 32-33 0.31642181097297595 1104 34-35 0.24649583592945845 1104 36-37 0.27577950632863946 1104 38-39 0.3131885765137028 1104 40-41 0.26157078258837885 1104 42-43 0.24309008323926662 1104 44-45 0.28230482437650295 1104 46-47 0.18343056694120463 1104 48-49 0.29604228167637103 1104 50-51 0.46765991866083567 1104 52-53 0.45976084800889794 1104 54-55 0.32371990271507656 1104 56-57 0.3031439768005768 1104 58-59 0.35984868061886033 1104 60-61 0.4645034592617421 1104 62-63 0.48508986765774864 1104 64-65 0.5359960719273431 1104 66-67 0.47436704869595303 1104 68-69 0.6576806603705094 1104 70-71 0.5854581268040349 1104 72-73 0.5467121540822575 1104 74-75 0.5418152838524364 1104 76-77 0.6549839210080322 1104 78-79 0.7978563717832401 1104 80-81 0.8524847693536373 1104 82-83 0.7196304095867099 1104 84-85 0.5061467041721421 1104 86-87 0.6341956050342681 1104 88-89 0.5773810299270785 1104 90-91 0.4992416118612333 1104 92-93 0.5842610851624848 1104 94-95 0.6026449828878278 1104 96-97 0.8432607708829387 1104 98-99 0.9807266263224044 1104 100-101 1.0476765458128519 1105 1 0.10543426811548073 1105 2 0.11235022440915543 1105 3 0.10139092883063228 1105 4 0.03477255614683372 1105 5 0.08207961324809787 1105 6 0.06197967001325111 1105 7 0.10981435947618934 1105 8 0.0768566246340967 1105 9 0.037371381637377965 1105 10-11 0.042200846275655124 1105 12-13 0.050091082949293764 1105 14-15 0.22743502558967776 1105 16-17 0.2550710206309432 1105 18-19 0.29583148288760697 1105 20-21 0.2822016877067668 1105 22-23 -0.07119709550527631 1105 24-25 0.11910908467619663 1105 26-27 0.07797208002821776 1105 28-29 0.011392664348008452 1105 30-31 0.09968953454367835 1105 32-33 -0.04495654240353275 1105 34-35 -0.10752243419079122 1105 36-37 -0.13199107646506292 1105 38-39 0.040703608891135445 1105 40-41 0.04131176316192864 1105 42-43 0.09780215539468173 1105 44-45 0.1433940935716862 1105 46-47 0.2900737214277882 1105 48-49 0.3869419116486199 1105 50-51 0.15849016842957298 1105 52-53 0.1863623743733669 1105 54-55 0.023714121031453317 1105 56-57 -0.013195061127262875 1105 58-59 0.33797078977704587 1105 60-61 0.2391565582441686 1105 62-63 0.2596967131711665 1105 64-65 0.3286591154056069 1105 66-67 0.17903864906597988 1105 68-69 0.16819754288670197 1105 70-71 0.09369124428784659 1105 72-73 0.19189601162156933 1105 74-75 -0.0432795357590372 1105 76-77 -0.12667079684580784 1105 78-79 0.1358651599423517 1105 80-81 0.3345152966431826 1105 82-83 0.011570742611691998 1105 84-85 -0.43260213949113435 1105 86-87 -0.25166239681587044 1105 88-89 -0.14196448348642932 1105 90-91 -0.11898803661240365 1105 92-93 0.0694484117119778 1105 94-95 -0.035589290932708906 1105 96-97 0.1824374591345581 1105 98-99 0.3882254699856773 1105 100-101 0.48747187421248483 1106 1 0.15864669900842898 1106 2 0.047643685066994124 1106 3 0.11064150123248595 1106 4 0.02444140073274781 1106 5 0.09379430582642101 1106 6 0.09686826106702995 1106 7 0.10683786983526744 1106 8 0.11719559821985115 1106 9 0.20284158881892722 1106 10-11 0.1069604374969586 1106 12-13 -0.08261819275294613 1106 14-15 0.10777651215917672 1106 16-17 0.07874611975419299 1106 18-19 0.18306901569600598 1106 20-21 0.16804242038113415 1106 22-23 0.21824767469632178 1106 24-25 0.10878840002773416 1106 26-27 0.2176939764932868 1106 28-29 0.2726187211972899 1106 30-31 0.18986027782842285 1106 32-33 0.25923106579438127 1106 34-35 0.1957276914583943 1106 36-37 0.3137117213235072 1106 38-39 0.43723499326596027 1106 40-41 0.32986931048979073 1106 42-43 0.3791572120764215 1106 44-45 0.3597996891488364 1106 46-47 0.33365095379501497 1106 48-49 0.43095612396782457 1106 50-51 0.18392575227945684 1106 52-53 0.229750434908361 1106 54-55 0.41878937093373025 1106 56-57 0.26709895797141314 1106 58-59 0.3231888609224072 1106 60-61 0.3091571166849931 1106 62-63 0.1804895595440854 1106 64-65 0.21553575582555595 1106 66-67 0.1556448645124604 1106 68-69 0.09524841684660856 1106 70-71 0.1302230474932955 1106 72-73 0.1202878416321802 1106 74-75 0.18720784347813435 1106 76-77 -0.003096217072535268 1106 78-79 0.22649382471032098 1106 80-81 0.4720599719098395 1106 82-83 0.38118039246928603 1106 84-85 0.3092948840192271 1106 86-87 0.3567435338257745 1106 88-89 0.238515915240324 1106 90-91 0.19297306798356217 1106 92-93 0.31975834637439604 1106 94-95 0.4992036671283806 1106 96-97 0.4198013391814719 1106 98-99 0.4239203099923792 1106 100-101 0.493581914978666 1107 1 0.10388491929502663 1107 2 0.09360520514122328 1107 3 0.07156930726802102 1107 4 0.0814020333473664 1107 5 0.10600650428878566 1107 6 0.11535947198480301 1107 7 0.09425264206518591 1107 8 0.05164074742484104 1107 9 0.05621656138040265 1107 10-11 0.12354152289104547 1107 12-13 0.11836259152433826 1107 14-15 0.13359175626443687 1107 16-17 0.12192527212926052 1107 18-19 0.1659379477413765 1107 20-21 0.27418393719640477 1107 22-23 0.17019988385130347 1107 24-25 0.17525885597189017 1107 26-27 0.21523723509955772 1107 28-29 0.18487360929523078 1107 30-31 0.16125943400111709 1107 32-33 0.22389805296998588 1107 34-35 0.18314381036451266 1107 36-37 0.0626401037364488 1107 38-39 0.12124886941753488 1107 40-41 0.20955079463469417 1107 42-43 0.03980760281451978 1107 44-45 0.13231012252270347 1107 46-47 0.24546642144852626 1107 48-49 0.33444212998207945 1107 50-51 0.30912906671893126 1107 52-53 0.2838204270572362 1107 54-55 0.34244508508285065 1107 56-57 0.3221512157413997 1107 58-59 0.45406087416292706 1107 60-61 0.3249179416049728 1107 62-63 0.39774936974354347 1107 64-65 0.3484641436252076 1107 66-67 0.3752624354048564 1107 68-69 0.44683688875965544 1107 70-71 0.28807652642925774 1107 72-73 0.38355253487954144 1107 74-75 0.3642097113180256 1107 76-77 0.45105646099509045 1107 78-79 0.3975518678012655 1107 80-81 0.5036801984477108 1107 82-83 0.42572857587042634 1107 84-85 0.5461491022804239 1107 86-87 0.6082332943732816 1107 88-89 0.48564446491045743 1107 90-91 0.3812629104089815 1107 92-93 0.3462362898382345 1107 94-95 0.48323801275821765 1107 96-97 0.3985851289707014 1107 98-99 0.39190752734187484 1107 100-101 0.6201477963984381 1108 1 0.08424443076668808 1108 2 0.03250921679215679 1108 3 0.05028663027087532 1108 4 0.012624495876082165 1108 5 -0.030451015514664448 1108 6 0.09391396646641681 1108 7 0.015533206836849445 1108 8 0.06770569274360128 1108 9 -0.05430951377579163 1108 10-11 0.04464615799607685 1108 12-13 0.06678445161426794 1108 14-15 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64-65 -0.7608552333802692 2316 66-67 -0.8286670716610871 2316 68-69 -1.00434484591608 2316 70-71 -0.7514815986974526 2316 72-73 -0.8692712656317312 2316 74-75 -0.768595984023559 2316 76-77 -0.7925284362239466 2316 78-79 -0.6177449335243708 2316 80-81 -0.6846025230928525 2316 82-83 -0.8714807815064809 2316 84-85 -1.1534595440282622 2316 86-87 -0.9725984730922335 2316 88-89 -0.9793470629801142 2316 90-91 -1.0109183123157557 2316 92-93 -0.9655217634794191 2316 94-95 -0.7585491096354495 2316 96-97 -1.0323222985095164 2316 98-99 -1.3898961541747674 2316 100-101 -1.9232785602433893 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 17.0 5 44.0 6 99.0 7 271.0 8 618.0 9 1190.0 10 2072.0 11 3272.0 12 4956.0 13 6837.0 14 8611.0 15 11516.0 16 15679.0 17 21281.0 18 18565.0 19 11332.0 20 9697.0 21 9919.0 22 10502.0 23 11649.0 24 13460.0 25 16143.0 26 19673.0 27 24234.0 28 30247.0 29 37391.0 30 46173.0 31 57098.0 32 71399.0 33 91479.0 34 122395.0 35 169762.0 36 251890.0 37 367068.0 38 264566.0 39 19263.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.925219166285224 19.910030532702223 15.31961047517982 21.845139825832735 2 15.271375583624907 21.5643547159635 40.02627026261121 23.13799943780038 3 18.91380760405525 26.392450337785334 30.508323506019803 24.185418552139613 4 12.762835934389066 17.806029437053347 37.60545573272988 31.825678895827703 5 13.218396058248699 38.296383223789455 33.81677758174655 14.668443136215298 6 30.333870359579063 37.26716484420526 17.8131034403958 14.58586135581988 7 27.309841291605252 34.005016053656924 21.15095007941133 17.5341925753265 8 26.269228022566594 34.60944083410698 20.026794258373208 19.094536884953225 9 26.9868828482958 16.465624607228143 20.56502016704944 35.98247237742662 10-11 24.46503045053074 27.44152698271233 27.27439184634194 20.819050720414996 12-13 25.97610456267682 24.991658934994536 28.062770513306855 20.96946598902179 14-15 22.825643574538102 25.63032027331208 27.241313314823067 24.30272283732675 16-17 21.28216839957758 29.135784060418935 27.51064983944901 22.071397700554478 18-19 21.20541848420704 27.198916760221792 30.884560119522025 20.711104636049146 20-21 22.402771116379288 26.09499346270428 30.87231834473444 20.629917076181993 22-23 21.945577531735967 26.298689427438614 30.743895611238703 21.01183742958672 24-25 21.680249626705464 26.344524504033178 30.965235259115115 21.009990610146243 26-27 21.551227909498614 26.637153150239833 30.799621658696953 21.011997281564604 28-29 21.30947099344173 26.65681464787413 30.93047299117518 21.103241367508964 30-31 21.55523063519581 26.37758342879496 30.864703752734908 21.202482183274324 32-33 21.379051088259978 26.588170568047886 30.69588993825852 21.336888405433616 34-35 21.726130112078508 26.518533203907236 30.65761636207922 21.097720321935036 36-37 21.832947550483652 26.30343844797017 30.533374549531146 21.330239452015032 38-39 21.618301629740007 26.656700462819995 30.52135168234682 21.203646225093177 40-41 21.815335890962604 26.525342841642484 30.358929909549744 21.300391357845168 42-43 21.637098960519037 26.63638906132472 30.1557464554319 21.570765522724344 44-45 21.76695405360703 26.738297725861937 29.87731041017556 21.617437810355472 46-47 22.11014882242946 26.96791653740614 29.331170326239288 21.590764313925114 48-49 22.123258029454067 27.233628654799535 28.819223556397926 21.823889759348475 50-51 22.03282586625242 27.351632009333116 28.800018510826835 21.815523613587633 52-53 22.311910430246304 27.448505218751052 28.410384826114953 21.82919952488769 54-55 22.08096154510856 27.392015137548242 28.44746149269046 22.079561824652732 56-57 22.21595684085379 27.656497895574567 28.162279852304582 21.96526541126706 58-59 22.462385584218556 27.350609974693768 28.195379292082567 21.991625149005102 60-61 22.27859729847156 27.274428202569784 28.689435633815407 21.757538865143246 62-63 22.20556233821361 27.314313071626202 28.79656348710765 21.68356110305254 64-65 22.140930854406875 27.380443601375827 28.564780427603388 21.91384511661391 66-67 22.138674544644843 27.624903270147446 28.16510503955033 22.071317145657375 68-69 22.12525155575361 27.521234947717822 28.4043402562048 21.949173240323763 70-71 22.13608463232456 27.36372982810666 28.406322228668735 22.09386331090004 72-73 22.1671665294401 27.47809184028542 28.25208396780385 22.102657662470637 74-75 22.037599438485188 27.81409449023591 28.019291930281437 22.129014140997466 76-77 22.086721680077222 27.694730133482793 28.09383303038535 22.12471515605463 78-79 22.107964524913353 27.650603992947037 28.01979332482376 22.22163815731585 80-81 22.033520178635648 27.82043997912452 27.9295592353311 22.216480606908735 82-83 21.989024013648137 27.696696891432232 28.063410588790166 22.250868506129464 84-85 22.032718890060075 27.673113893387953 27.878728150985648 22.415439065566325 86-87 22.070236177287217 27.59469372364858 27.90805425107691 22.427015847987295 88-89 22.059518348034192 27.52725024866441 27.780256645667066 22.632974757634326 90-91 21.905295674643916 27.541044577865147 27.901286197698404 22.65237354979253 92-93 21.974478626306578 27.53956864125194 27.86329908545364 22.622653646987846 94-95 21.84466976220708 27.48614219585316 27.853122272894122 22.816065769045643 96-97 21.863337323905643 27.62218735351357 27.743447048933213 22.77102827364757 98-99 21.737659944114895 27.68732107426 27.503846314251156 23.071172667373943 100-101 21.574036965422362 27.72966560412668 27.65379573580489 23.042501694646063 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1436.0 1 980.0 2 609.0 3 680.5 4 587.5 5 563.5 6 919.5 7 1948.0 8 3178.5 9 3634.5 10 3146.5 11 2612.5 12 2556.5 13 2718.0 14 2949.5 15 3163.5 16 3381.0 17 3564.5 18 3822.0 19 4039.0 20 4354.5 21 4959.5 22 5803.5 23 6845.0 24 8046.5 25 9808.5 26 12067.0 27 14488.5 28 16633.5 29 18945.0 30 21963.5 31 25161.0 32 28365.0 33 31516.5 34 35039.0 35 38284.5 36 41744.5 37 46018.0 38 48757.0 39 49934.0 40 51412.0 41 53853.0 42 56178.0 43 58673.5 44 61617.0 45 63679.0 46 63932.0 47 64924.5 48 69211.5 49 69315.0 50 64872.5 51 61908.0 52 59557.0 53 57563.0 54 54963.0 55 50314.0 56 45200.5 57 40535.5 58 36842.5 59 33306.5 60 28358.5 61 22968.0 62 18066.5 63 13612.0 64 10021.5 65 7572.0 66 5351.5 67 3871.0 68 2798.5 69 1758.5 70 1136.0 71 729.5 72 521.5 73 415.0 74 301.5 75 235.5 76 158.5 77 60.5 78 30.0 79 23.5 80 12.5 81 3.5 82 3.5 83 3.0 84 1.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02588009460802797 2 0.006398610587415304 3 0.008912350461042745 4 5.7130451673350926E-5 5 0.005256001553948285 6 0.0 7 0.0 8 2.8565225836675465E-4 9 0.0 10-11 0.0 12-13 2.285218066934037E-4 14-15 0.0 16-17 8.569567751002639E-5 18-19 0.004284783875501319 20-21 0.0021995223894240106 22-23 0.0 24-25 0.004941784069744855 26-27 0.006198654006558575 28-29 0.00836961117014591 30-31 0.03139318319450633 32-33 9.712176784469658E-4 34-35 0.005084610198928233 36-37 0.01479678698339789 38-39 0.007512654395045647 40-41 0.010940481495446703 42-43 0.007512654395045647 44-45 0.003913435939624539 46-47 0.0022280876152606863 48-49 0.0015710874210171505 50-51 0.003027913938687599 52-53 0.003256435745381003 54-55 0.0017424787760372035 56-57 0.0011997394851403695 58-59 0.001171174259303694 60-61 2.8565225836675463E-5 62-63 5.7130451673350926E-5 64-65 0.009140872267736149 66-67 8.855220009369395E-4 68-69 0.001285435162650396 70-71 0.004541870908031399 72-73 0.013311395239890767 74-75 0.006541436716598682 76-77 0.004541870908031399 78-79 0.011083307624630081 80-81 8.569567751002639E-5 82-83 0.0059701321998651725 84-85 8.56956775100264E-4 86-87 0.0 88-89 0.001656783098527177 90-91 0.001542522195180475 92-93 2.285218066934037E-4 94-95 7.141306459168867E-4 96-97 2.5708703253007914E-4 98-99 1.7139135502005278E-4 100-101 8.283915492635885E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1750380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.24887020282749 #Duplication Level Percentage of deduplicated Percentage of total 1 74.48270001047011 40.40602325224131 2 14.238773539960825 15.448747552335785 3 4.868439893622147 7.923220916381253 4 2.1593747699231782 4.685745664512919 5 1.1629417316002737 3.1544137525517346 6 0.6973268620314005 2.2697516656371866 7 0.4607484771251943 1.7496559032200567 8 0.3245333590957375 1.4084454459257816 9 0.23119910802427748 1.1288061361996669 >10 1.241034426293971 12.176264249600676 >50 0.09150699987212838 3.3960755907537754 >100 0.038470137891803965 3.543757824594092 >500 0.002002249917477709 0.6976820994754553 >1k 6.322894476245397E-4 0.536810464935638 >5k 2.107631492081799E-4 0.8220231816091096 >10k+ 1.0538157460408995E-4 0.6525763000255919 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11415 0.6521441058513009 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8318 0.47521109701893305 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6061 0.34626766759218 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2074 0.11848855677052983 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1874 0.10706246643585964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 8.56956775100264E-5 14-15 0.0 0.0 0.0 0.0 1.1426090334670186E-4 16-17 0.0 0.0 0.0 0.0 1.1426090334670186E-4 18-19 0.0 0.0 0.0 0.0 1.1426090334670186E-4 20-21 0.0 0.0 0.0 8.56956775100264E-5 1.1426090334670186E-4 22-23 1.7139135502005278E-4 0.0 0.0 3.713479358767811E-4 1.4282612918337732E-4 24-25 1.7139135502005278E-4 0.0 0.0 6.855654200802111E-4 1.7139135502005278E-4 26-27 1.9995658085672827E-4 0.0 0.0 0.0013140003884870714 1.7139135502005278E-4 28-29 2.570870325300792E-4 0.0 0.0 0.0020566962602406335 1.7139135502005278E-4 30-31 2.8565225836675465E-4 0.0 0.0 0.004399044778848022 1.7139135502005278E-4 32-33 2.8565225836675465E-4 0.0 0.0 0.0077411762017390515 1.7139135502005278E-4 34-35 2.8565225836675465E-4 0.0 0.0 0.011311829431323483 1.7139135502005278E-4 36-37 2.8565225836675465E-4 0.0 0.0 0.01442543904752111 1.7139135502005278E-4 38-39 3.4278271004010557E-4 0.0 0.0 0.016796352791965175 1.7139135502005278E-4 40-41 3.4278271004010557E-4 0.0 0.0 0.0199099624081628 1.7139135502005278E-4 42-43 4.28478387550132E-4 0.0 0.0 0.023709137444440635 1.7139135502005278E-4 44-45 5.141740650601584E-4 0.0 0.0 0.02727979067402507 1.7139135502005278E-4 46-47 6.855654200802111E-4 0.0 0.0 0.032678618357156734 2.2852180669340373E-4 48-49 7.426958717535621E-4 0.0 0.0 0.03862018533118523 3.142174842034301E-4 50-51 7.426958717535621E-4 0.0 0.0 0.04364766507844011 3.4278271004010557E-4 52-53 7.426958717535621E-4 0.0 0.0 0.05573075560735383 4.28478387550132E-4 54-55 7.99826323426913E-4 0.0 0.0 0.0797541105359979 5.141740650601584E-4 56-57 8.569567751002639E-4 0.0 0.0 0.12405877580868155 5.141740650601584E-4 58-59 8.569567751002639E-4 0.0 0.0 0.17379083399033352 5.141740650601584E-4 60-61 8.569567751002639E-4 0.0 0.0 0.2195808910065243 5.141740650601584E-4 62-63 8.569567751002639E-4 0.0 0.0 0.2768827340348953 5.141740650601584E-4 64-65 8.569567751002639E-4 0.0 0.0 0.34315405797598236 5.141740650601584E-4 66-67 8.569567751002639E-4 0.0 0.0 0.44233252208091955 5.141740650601584E-4 68-69 9.140872267736149E-4 0.0 0.0 0.5671625589871914 5.141740650601584E-4 70-71 9.140872267736149E-4 0.0 0.0 0.6734537643254608 5.141740650601584E-4 72-73 9.140872267736149E-4 0.0 0.0 0.7782310126943863 5.427392908968338E-4 74-75 9.140872267736149E-4 0.0 0.0 0.8886641757789737 6.570001942435357E-4 76-77 9.140872267736149E-4 0.0 0.0 1.0218638238553914 6.855654200802111E-4 78-79 9.140872267736149E-4 0.0 0.0 1.1317828128749186 6.855654200802111E-4 80-81 0.0010283481301203168 0.0 0.0 1.2533564140358093 6.855654200802111E-4 82-83 0.0010283481301203168 0.0 0.0 1.3977821958660406 8.569567751002639E-4 84-85 0.0010569133559569922 0.0 0.0 1.6090791713799288 8.569567751002639E-4 86-87 0.0010854785817936677 0.0 0.0 1.860538854420183 8.855220009369395E-4 88-89 0.0010854785817936677 0.0 0.0 2.1560746809264275 9.140872267736149E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12100 0.0 36.995026 1 GGTATCA 7255 0.0 36.67992 1 ATCAACG 15245 0.0 29.288998 3 TATCAAC 15350 0.0 29.185654 2 TCAACGC 15445 0.0 28.906425 4 CAACGCA 15615 0.0 28.591719 5 AACGCAG 15995 0.0 27.91086 6 ACGCAGA 18045 0.0 24.66109 7 CGCAGAG 18125 0.0 24.473635 8 GTACATG 9655 0.0 23.723164 1 TACATGG 9835 0.0 22.993162 2 AGTACTT 10265 0.0 22.972841 12-13 GAGTACT 9905 0.0 22.896723 12-13 GCAGAGT 18965 0.0 22.888798 9 AGAGTAC 15490 0.0 21.876217 10-11 ACATGGG 9875 0.0 21.597988 3 GTACTTT 10655 0.0 21.13053 14-15 TACTTTT 11605 0.0 20.137493 14-15 CGATCGT 215 0.0 18.78462 24-25 ACTTTTT 12290 0.0 18.590233 16-17 >>END_MODULE