FastQCFastQC Report
Thu 26 May 2016
SRR937125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784144
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109430.613347353128447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76000.4259745850110753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50990.28579531696993066No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19560.10963240635285043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72900.037.219731
GTATCAA118650.036.2447781
ATCAACG145950.029.0744823
TATCAAC147600.028.8782392
TCAACGC147750.028.7202744
CAACGCA149300.028.4857625
AACGCAG153050.027.8793896
GTACATG105100.025.2287731
ACGCAGA169500.024.9206947
CGCAGAG172000.024.448668
TACATGG107300.024.2243542
GAGTACT87950.023.3923612-13
ACATGGG107650.023.2186163
GCAGAGT177050.023.1346959
AGTACTT91700.021.60671412-13
ATACCGT1100.002872348321.5982026
GTACTTT96850.021.28730814-15
AGAGTAC147150.021.27999510-11
CATGGGA70200.020.7132844
CATGGGG39350.020.1667694
Produced by FastQC (version 0.11.5)