##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937125_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1784144 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50659588015317 31.0 31.0 34.0 30.0 34.0 2 31.602119559856156 33.0 31.0 34.0 30.0 34.0 3 31.599486364329337 33.0 31.0 34.0 28.0 34.0 4 35.1971124528065 37.0 35.0 37.0 33.0 37.0 5 35.19134834407985 37.0 35.0 37.0 33.0 37.0 6 35.29386305141289 37.0 35.0 37.0 33.0 37.0 7 35.22144569048238 37.0 35.0 37.0 32.0 37.0 8 35.18279185984988 37.0 35.0 37.0 32.0 37.0 9 36.72951846936122 39.0 37.0 39.0 32.0 39.0 10-11 36.59200322395502 39.0 37.0 39.0 32.0 39.0 12-13 36.47480472428234 39.0 37.0 39.0 32.0 39.0 14-15 37.5526507389538 40.0 37.0 41.0 32.0 41.0 16-17 37.47725660036409 40.0 37.0 41.0 32.0 41.0 18-19 37.43800304235532 40.0 37.0 41.0 32.0 41.0 20-21 37.329257335730745 40.0 37.0 41.0 31.5 41.0 22-23 37.196972609834184 39.5 36.5 41.0 31.0 41.0 24-25 37.30559724999776 40.0 37.0 41.0 31.0 41.0 26-27 37.24812739330457 40.0 37.0 41.0 31.0 41.0 28-29 37.13914992287618 40.0 36.5 41.0 31.0 41.0 30-31 36.92432253226197 39.0 36.0 41.0 30.5 41.0 32-33 36.688903474159034 39.0 35.5 41.0 30.0 41.0 34-35 36.47962160005022 39.0 35.0 41.0 30.0 41.0 36-37 36.2688916925988 39.0 35.0 41.0 30.0 41.0 38-39 36.085583338564604 39.0 35.0 40.5 29.5 41.0 40-41 35.8698347779103 39.0 35.0 40.0 28.5 41.0 42-43 35.601373263593075 38.0 35.0 40.0 27.0 41.0 44-45 35.27760987902322 38.0 35.0 40.0 26.5 41.0 46-47 34.85386409392964 38.0 34.0 40.0 24.5 41.0 48-49 34.53940909478159 38.0 34.0 40.0 23.5 41.0 50-51 33.80898907263091 37.5 33.0 39.5 22.5 40.5 52-53 33.997448356186496 38.0 33.5 39.5 23.0 40.5 54-55 34.39024344447533 38.0 34.0 40.0 23.0 41.0 56-57 34.28469422871696 38.0 34.0 40.0 22.5 41.0 58-59 34.02346923790905 38.0 34.0 40.0 22.0 41.0 60-61 33.73052343308612 38.0 33.0 40.0 20.5 41.0 62-63 33.390815147207846 37.0 33.0 40.0 19.5 41.0 64-65 33.04527241074712 37.0 32.5 40.0 18.0 41.0 66-67 32.66114450403107 36.0 32.0 39.0 16.0 41.0 68-69 32.26173980351362 36.0 31.5 39.0 13.0 41.0 70-71 31.858024352294436 35.0 31.0 38.5 9.5 40.0 72-73 31.457526130177833 35.0 31.0 38.0 8.0 40.0 74-75 31.005549720201955 35.0 31.0 37.0 7.0 39.0 76-77 30.569626947152248 35.0 30.0 36.5 3.5 39.0 78-79 30.130357751392264 34.5 30.0 36.0 2.0 39.0 80-81 29.686935023181984 34.0 29.0 36.0 2.0 37.5 82-83 29.265750410280784 34.0 29.0 35.0 2.0 37.0 84-85 28.854264566088837 34.0 29.0 35.0 2.0 36.5 86-87 28.487191897066605 34.0 29.0 35.0 2.0 36.0 88-89 28.12100901048346 34.0 27.5 35.0 2.0 36.0 90-91 27.719154956102198 34.0 27.0 35.0 2.0 35.5 92-93 27.323010082145835 34.0 26.0 35.0 2.0 35.0 94-95 26.879815754782125 33.0 25.5 35.0 2.0 35.0 96-97 26.338342644988295 33.0 25.0 35.0 2.0 35.0 98-99 25.642780795720526 33.0 22.0 35.0 2.0 35.0 100-101 23.112599095140304 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4826471705417035 1101 2 0.598559460344326 1101 3 0.5449914500011204 1101 4 0.43785365027237333 1101 5 0.505273699428578 1101 6 0.42910055247436674 1101 7 0.35922270447845506 1101 8 0.34780596670705677 1101 9 0.5471858018219677 1101 10-11 0.5007397607161153 1101 12-13 0.6325995623815004 1101 14-15 0.7057104557970391 1101 16-17 0.756566235314331 1101 18-19 0.7563940990008646 1101 20-21 0.7595263650674866 1101 22-23 0.8448103545014689 1101 24-25 0.914859399098674 1101 26-27 0.7965459872240856 1101 28-29 0.7257148008153109 1101 30-31 0.896663983267473 1101 32-33 1.041242160406064 1101 34-35 0.9505197659013689 1101 36-37 0.8787555983201187 1101 38-39 1.003815181083958 1101 40-41 0.9828640753501006 1101 42-43 0.9705205394353555 1101 44-45 1.124020847175764 1101 46-47 1.1385384518548989 1101 48-49 1.1300048820357205 1101 50-51 1.0172380931015041 1101 52-53 1.1534528053898896 1101 54-55 1.0865131087490312 1101 56-57 0.9997379492828387 1101 58-59 0.9750080393444165 1101 60-61 1.2833591764542547 1101 62-63 1.249299343334961 1101 64-65 1.21850655707766 1101 66-67 1.2725824467653482 1101 68-69 1.4541631678197007 1101 70-71 1.2637967203040823 1101 72-73 1.1407167058804397 1101 74-75 1.0367651214073668 1101 76-77 1.0374249862040195 1101 78-79 1.1887946930626256 1101 80-81 1.1656925778486915 1101 82-83 1.4091961867314744 1101 84-85 1.3250357555668835 1101 86-87 1.225043684553068 1101 88-89 1.291068618531849 1101 90-91 1.1708214537694666 1101 92-93 1.3105746746175306 1101 94-95 1.5708383439017517 1101 96-97 1.6382786862889276 1101 98-99 1.4358931170449765 1101 100-101 1.3970706682434795 1102 1 0.3431458655628532 1102 2 0.3850244314728357 1102 3 0.25887468086714094 1102 4 0.22523366385162547 1102 5 0.3460370030495028 1102 6 0.29725221655598943 1102 7 0.25394299051487224 1102 8 0.26935088725596046 1102 9 0.42599904969320335 1102 10-11 0.38673724419364675 1102 12-13 0.24191536726380036 1102 14-15 0.336946479442382 1102 16-17 0.5366262207384338 1102 18-19 0.4863525514641296 1102 20-21 0.5706510761968673 1102 22-23 0.4236394863635127 1102 24-25 0.1436309125626991 1102 26-27 0.40370904417598297 1102 28-29 0.24379576556530225 1102 30-31 0.11371902908189213 1102 32-33 0.40818850989123234 1102 34-35 0.3271002229865658 1102 36-37 0.220108166854871 1102 38-39 0.43106712609421294 1102 40-41 0.4427588672698235 1102 42-43 0.4544677422957193 1102 44-45 0.26106231373528743 1102 46-47 0.41347102275667424 1102 48-49 0.2856555339022435 1102 50-51 0.38476187237009185 1102 52-53 0.2545727824671147 1102 54-55 0.3622436166007006 1102 56-57 0.3637286306748919 1102 58-59 0.28378503434251456 1102 60-61 0.22070648769949486 1102 62-63 0.43972316558375013 1102 64-65 0.4845054732288787 1102 66-67 0.3847925639331393 1102 68-69 0.37898797674463225 1102 70-71 0.31147329733520834 1102 72-73 0.23691015420140715 1102 74-75 0.4412465869702551 1102 76-77 0.558673405591211 1102 78-79 0.41646460504663096 1102 80-81 0.33983780112777495 1102 82-83 0.29663817901995415 1102 84-85 0.2811258645123722 1102 86-87 0.1115142617959819 1102 88-89 0.17343271697521345 1102 90-91 0.10112748532566584 1102 92-93 0.08270107381024872 1102 94-95 0.25131660775041453 1102 96-97 0.295896149958196 1102 98-99 0.3656537982501682 1102 100-101 0.43882999152317836 1103 1 0.16895728000885057 1103 2 0.30577166818957124 1103 3 0.22590245921517393 1103 4 0.34078618163047025 1103 5 0.2747163390162015 1103 6 0.16093265747371532 1103 7 0.25020728996988595 1103 8 0.3655075898405755 1103 9 0.2886645055428616 1103 10-11 0.3895823421357818 1103 12-13 0.4536034546841492 1103 14-15 0.5962247471689963 1103 16-17 0.5317282083219226 1103 18-19 0.6320798930244251 1103 20-21 0.5900462875927133 1103 22-23 0.6756350399922795 1103 24-25 0.3488124686960177 1103 26-27 0.40174428426433195 1103 28-29 0.4180220885177377 1103 30-31 0.5615713786424976 1103 32-33 0.5223966705885417 1103 34-35 0.4234679531031844 1103 36-37 0.6765993616631718 1103 38-39 0.7488610038202026 1103 40-41 0.7748081227260428 1103 42-43 0.6277107795947572 1103 44-45 0.4762216380124542 1103 46-47 0.6465882163984347 1103 48-49 0.6795868771966411 1103 50-51 0.6856705285443567 1103 52-53 0.5693416303352805 1103 54-55 0.5253238215540321 1103 56-57 0.700221238371391 1103 58-59 0.8503042936992813 1103 60-61 0.8334374556728221 1103 62-63 0.7334426964082681 1103 64-65 0.652057710845277 1103 66-67 0.454833175411828 1103 68-69 0.5371239787057647 1103 70-71 0.5526194998660863 1103 72-73 0.3674631369049983 1103 74-75 0.45846238813220097 1103 76-77 0.43863104309723155 1103 78-79 0.2694182659365545 1103 80-81 0.46406418446003883 1103 82-83 0.539683931238006 1103 84-85 0.5324122569499217 1103 86-87 0.5051047609077948 1103 88-89 0.37869276689726306 1103 90-91 0.27808780731925964 1103 92-93 0.4877090982906509 1103 94-95 0.7349041603261561 1103 96-97 0.5264770885265406 1103 98-99 0.6506049690755944 1103 100-101 0.771486668144469 1104 1 0.479909534427275 1104 2 0.4200253477815252 1104 3 0.3937443485584531 1104 4 0.38835677600366125 1104 5 0.5168986238305564 1104 6 0.4156232865851095 1104 7 0.4848035556134178 1104 8 0.4397021626449771 1104 9 0.47621513841323804 1104 10-11 0.6574375175442455 1104 12-13 0.5228643605993639 1104 14-15 0.6994574736640047 1104 16-17 0.6860134504199564 1104 18-19 0.6206279529348606 1104 20-21 0.7771108449075115 1104 22-23 0.6971276808083715 1104 24-25 0.695530162494336 1104 26-27 0.5825935554750501 1104 28-29 0.6903475387857227 1104 30-31 0.49977011225365686 1104 32-33 0.6684980205823834 1104 34-35 0.7161551083946023 1104 36-37 0.5236038400392928 1104 38-39 0.4849770657008676 1104 40-41 0.5335104954228527 1104 42-43 0.7113433194367502 1104 44-45 0.7094504110929378 1104 46-47 0.6401493355387942 1104 48-49 0.6873203392859892 1104 50-51 0.6157177750883562 1104 52-53 0.42576949348232773 1104 54-55 0.5208324834204276 1104 56-57 0.5469632359474943 1104 58-59 0.6505094175837343 1104 60-61 0.7719035308395448 1104 62-63 0.8233942184728562 1104 64-65 0.7388432607911568 1104 66-67 0.6263757199849564 1104 68-69 0.6478617117790009 1104 70-71 0.6015478652912059 1104 72-73 0.5919862967849703 1104 74-75 0.3793908572534441 1104 76-77 0.3916534609018534 1104 78-79 0.44038886160594615 1104 80-81 0.571287591266394 1104 82-83 0.446856462136207 1104 84-85 0.5458830308470155 1104 86-87 0.5665900781855093 1104 88-89 0.6664494958032066 1104 90-91 0.5176744486667921 1104 92-93 0.3268067476292629 1104 94-95 0.5315026035881765 1104 96-97 0.5556610648838216 1104 98-99 0.5260643104756255 1104 100-101 0.502502177195197 1105 1 0.24166796431529391 1105 2 0.34596961577016216 1105 3 0.33669152267383495 1105 4 0.3006950362113727 1105 5 0.4880496799241598 1105 6 0.3668915363691525 1105 7 0.2174786765346255 1105 8 0.3526135012265996 1105 9 0.3721389161994466 1105 10-11 0.32234612075080804 1105 12-13 0.47021062269985237 1105 14-15 0.4636062876652005 1105 16-17 0.5283451930469383 1105 18-19 0.5342945608242573 1105 20-21 0.4170975139996642 1105 22-23 0.340712551092885 1105 24-25 0.26851911497180225 1105 26-27 0.15288814677585805 1105 28-29 0.19040054993033806 1105 30-31 -0.009520230343426306 1105 32-33 0.28833320056359213 1105 34-35 -0.11302373223492879 1105 36-37 0.16227764628411734 1105 38-39 0.05037231121500696 1105 40-41 0.109023615402144 1105 42-43 0.26009376305633225 1105 44-45 0.12639882734314511 1105 46-47 0.15627909231250214 1105 48-49 0.03566954434202785 1105 50-51 0.25385547125749497 1105 52-53 0.31631822021044087 1105 54-55 0.2482406681179512 1105 56-57 0.3728765900686355 1105 58-59 0.44582320449902113 1105 60-61 0.4266422249574333 1105 62-63 0.5233085915239997 1105 64-65 0.5809933704963299 1105 66-67 0.4750386673153173 1105 68-69 0.513938820115925 1105 70-71 0.32141270361279695 1105 72-73 0.23169884245825756 1105 74-75 0.13102485603474534 1105 76-77 0.17595822811038886 1105 78-79 0.3692328392051145 1105 80-81 0.3599130457672075 1105 82-83 0.25701922867066074 1105 84-85 0.25107661638691425 1105 86-87 0.26886093611787487 1105 88-89 0.33118483626867956 1105 90-91 0.28991415836660295 1105 92-93 0.27055076295004454 1105 94-95 0.0688961069886993 1105 96-97 -0.1743385277673788 1105 98-99 -0.2860329443283014 1105 100-101 -0.09275954719595347 1106 1 0.0035958057322140746 1106 2 0.19957182387807038 1106 3 0.3047303048898016 1106 4 0.21586325655584204 1106 5 0.32518175189275667 1106 6 0.2804594829669682 1106 7 0.2984838319527796 1106 8 0.3334712047770978 1106 9 0.2662925467968975 1106 10-11 0.33276031369471326 1106 12-13 0.4099080980993577 1106 14-15 0.41263797362510246 1106 16-17 0.40421522383264374 1106 18-19 0.28754044490938213 1106 20-21 0.4875297984025835 1106 22-23 0.4222320609395638 1106 24-25 0.47789402052056573 1106 26-27 0.28076998882317383 1106 28-29 0.42289846660600716 1106 30-31 0.3941589420263014 1106 32-33 0.5111525644696258 1106 34-35 0.23849838986298977 1106 36-37 0.1758710677287567 1106 38-39 0.22951904417384839 1106 40-41 0.2109157164725204 1106 42-43 0.2747466574738837 1106 44-45 0.37392825295011534 1106 46-47 0.5282202216685903 1106 48-49 0.614150692955377 1106 50-51 0.5068393206363808 1106 52-53 0.3728928895771517 1106 54-55 0.3549908008894036 1106 56-57 0.47008633873120687 1106 58-59 0.37102619369164813 1106 60-61 0.44761633560462855 1106 62-63 0.5227027543298632 1106 64-65 0.5009224727022783 1106 66-67 0.430602870405977 1106 68-69 0.30032359392950525 1106 70-71 0.39888052542704244 1106 72-73 0.2525229105754079 1106 74-75 0.4315819024205574 1106 76-77 0.4692997400209613 1106 78-79 0.45519533504925747 1106 80-81 0.3957880527835833 1106 82-83 0.4598608075066757 1106 84-85 0.5306787122327883 1106 86-87 0.5159191850028115 1106 88-89 0.36424627598612247 1106 90-91 0.484408407096101 1106 92-93 0.3422754100802372 1106 94-95 0.2798170442903505 1106 96-97 0.3286053822330075 1106 98-99 0.15123524535999877 1106 100-101 0.07092147102071777 1107 1 0.2122130542898084 1107 2 0.21945613082797166 1107 3 0.16639102984162335 1107 4 0.09676805370124697 1107 5 0.22282307982443683 1107 6 0.24202876114387095 1107 7 0.22937389779553286 1107 8 0.1515396170539347 1107 9 0.2328750294802333 1107 10-11 0.13662522791774023 1107 12-13 0.18771242561404478 1107 14-15 0.2634663611941406 1107 16-17 0.23161627858318212 1107 18-19 0.15212811064995435 1107 20-21 0.4165749631275233 1107 22-23 0.29283688690357934 1107 24-25 0.3380047387373111 1107 26-27 0.19791325382723812 1107 28-29 0.38023457482660916 1107 30-31 0.3652360819962155 1107 32-33 0.30970615984661976 1107 34-35 0.36768963329763693 1107 36-37 0.516163488111296 1107 38-39 0.22004566275224846 1107 40-41 0.28994137362546013 1107 42-43 0.3573267445093222 1107 44-45 0.40405336437403605 1107 46-47 0.4292222454114807 1107 48-49 0.2720673057324845 1107 50-51 0.2567276954651163 1107 52-53 0.4830621036769074 1107 54-55 0.48922302095842696 1107 56-57 0.5219045678730012 1107 58-59 0.41435151827671746 1107 60-61 0.5290201992308639 1107 62-63 0.40248244488127227 1107 64-65 0.44813785400688744 1107 66-67 0.47860249903762053 1107 68-69 0.5298622438767211 1107 70-71 0.4830230965303919 1107 72-73 0.3377447038874486 1107 74-75 0.3895449630754193 1107 76-77 0.34259685241730864 1107 78-79 0.48811550319540586 1107 80-81 0.43602879285846896 1107 82-83 0.3775730657214602 1107 84-85 0.5485368613264505 1107 86-87 0.3880112285430677 1107 88-89 0.3901512314460689 1107 90-91 0.4295911906307417 1107 92-93 0.2766619465819389 1107 94-95 0.3386837590059315 1107 96-97 0.2507740607466147 1107 98-99 0.23689777026770642 1107 100-101 0.09874431328737643 1108 1 -0.0601182297288787 1108 2 0.22065084292832893 1108 3 0.1302621196639393 1108 4 0.18308971323797607 1108 5 0.23784231765748132 1108 6 0.16409976859783626 1108 7 0.144983442135306 1108 8 0.15650583489966863 1108 9 0.27003725431615067 1108 10-11 0.1478609015758181 1108 12-13 0.2575715460536401 1108 14-15 0.42372103522695426 1108 16-17 0.4166974039871434 1108 18-19 0.5393224294599932 1108 20-21 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-2.0493377523615983 2316 88-89 -2.1397661677220974 2316 90-91 -2.140395569696391 2316 92-93 -2.5642945625276923 2316 94-95 -2.090545884525948 2316 96-97 -2.187916281365723 2316 98-99 -2.3158727769537464 2316 100-101 -2.562766795195323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4231.0 3 1661.0 4 1732.0 5 1978.0 6 2185.0 7 2517.0 8 3292.0 9 3940.0 10 5037.0 11 6531.0 12 8221.0 13 10095.0 14 11183.0 15 13608.0 16 17275.0 17 21993.0 18 18965.0 19 12426.0 20 11983.0 21 12675.0 22 13869.0 23 15483.0 24 17295.0 25 19652.0 26 22652.0 27 26678.0 28 31623.0 29 37918.0 30 45615.0 31 56051.0 32 69420.0 33 89042.0 34 118208.0 35 163751.0 36 236143.0 37 327033.0 38 276948.0 39 45213.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.26265444176708 19.1103010514206 14.62390717627918 22.003137330533132 2 15.083362341847542 20.86886525979577 40.84852846954442 23.199243928812265 3 18.768617602708627 26.501992791196965 30.079879815912058 24.64950979018235 4 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30.357559254085736 21.135844377728404 24-25 21.76996061055738 26.375792938486047 30.65151623430763 21.20273021664894 26-27 21.660275179581916 26.596395806616506 30.42002215067842 21.323306863123157 28-29 21.367140032195433 26.70393654181595 30.59619842789434 21.33272499809428 30-31 21.630709180424898 26.475413419544612 30.47489440314235 21.418982996888143 32-33 21.434382550028992 26.659643677165562 30.33654019960872 21.56943357319673 34-35 21.838165953672423 26.600201497357972 30.24295985326284 21.318672695706763 36-37 21.900614524388164 26.360176084441616 30.151293841752686 21.587915549417534 38-39 21.63010704594173 26.6872199807134 30.254652029927016 21.428020943417852 40-41 21.831820142269947 26.603941891445395 30.043435407521873 21.52080255876279 42-43 21.65974503703882 26.706149708417186 29.846623712300847 21.787481542243146 44-45 21.861432554135266 26.774970826313155 29.58273500651854 21.780861613033043 46-47 22.0903413625806 27.019147557596245 29.106338950219264 21.78417212960389 48-49 22.03061988022207 27.206209171391176 28.67462383732173 22.088547111065026 50-51 21.97084364759594 27.37350414583613 28.654822114526358 22.00083009204157 52-53 22.26751974351645 27.46016265630868 28.2462600819447 22.026057518230175 54-55 22.04354609400376 27.34518256867081 28.328922636194697 22.282348701130733 56-57 22.115074457420814 27.684675057821963 28.06771664002919 22.132533844728037 58-59 22.424099071894606 27.259400090296694 28.12753188759603 22.18896895021267 60-61 22.244683248153947 27.18608715538222 28.622543408976515 21.94668618748732 62-63 22.17530151799439 27.307924594928245 28.63607510239771 21.88069878467965 64-65 22.081660701053856 27.4147560077439 28.4428853761587 22.060697915043544 66-67 22.08366675121817 27.637047012972815 28.059879282872437 22.219406952936577 68-69 22.023403604689914 27.431980068059875 28.350236185866628 22.194380141383583 70-71 22.069975838984874 27.367878997016525 28.30889433151021 22.253250832488394 72-73 22.10012750758136 27.43725458154164 28.1505850801274 22.312032830749594 74-75 21.97985594249136 27.831375856562516 27.970208568610357 22.218559632335772 76-77 22.03791137043958 27.662466689205594 27.99856019995728 22.30106174039755 78-79 21.99551061687533 27.655984760315828 27.95801462525312 22.39048999755573 80-81 21.914452530513415 27.78860032501287 27.93989168480705 22.357055459666668 82-83 21.95719017939845 27.692714839676235 27.993160700548803 22.356934280376514 84-85 22.065167881083823 27.59996692412591 27.794189578016315 22.540675616773953 86-87 22.06370752847785 27.586144073628006 27.874166882254 22.475981515640147 88-89 22.0993720993721 27.530003402693342 27.716590960944142 22.65403353699042 90-91 22.002046863704162 27.481149484121385 27.899520352165958 22.61728330000849 92-93 22.070283097547197 27.461989910698893 27.838117037574868 22.629609954179042 94-95 21.946164201455833 27.388042487224094 27.880888456882563 22.784904854437503 96-97 21.980086954547453 27.514238519838624 27.80894553806455 22.69672898754937 98-99 21.870761857827727 27.575262677213562 27.56205943644868 22.99191602851003 100-101 22.016834710994036 27.45209026509175 27.646151465299436 22.88492355861478 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 893.0 1 645.0 2 486.5 3 574.5 4 521.5 5 558.5 6 891.5 7 1762.5 8 3069.5 9 3638.5 10 3066.0 11 2576.5 12 2604.5 13 2749.0 14 2934.5 15 3119.0 16 3264.5 17 3410.5 18 3591.5 19 3760.0 20 4092.0 21 4737.0 22 5596.5 23 6655.0 24 7930.0 25 9677.5 26 11941.5 27 14525.0 28 16709.0 29 19108.5 30 22423.0 31 25711.5 32 28770.0 33 31878.5 34 35798.0 35 39029.5 36 42564.0 37 47108.0 38 49808.0 39 50927.0 40 52345.5 41 54903.5 42 57077.5 43 59305.5 44 62286.5 45 64355.0 46 64822.0 47 66115.5 48 70337.0 49 70720.5 50 66532.0 51 63443.5 52 61196.5 53 59137.5 54 56088.5 55 51310.0 56 46317.5 57 41954.5 58 38256.5 59 34372.0 60 29506.0 61 24234.0 62 19254.0 63 14501.5 64 10455.5 65 7874.0 66 5669.5 67 4083.5 68 2922.5 69 1871.5 70 1299.0 71 893.0 72 583.5 73 433.5 74 335.5 75 258.0 76 185.5 77 91.0 78 45.0 79 25.5 80 16.5 81 13.0 82 11.5 83 8.0 84 6.5 85 4.5 86 4.0 87 3.5 88 2.5 89 3.5 90 2.5 91 1.0 92 1.5 93 2.0 94 1.0 95 0.0 96 1.5 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025894770825673267 2 0.010369118187769597 3 0.01199454752531186 4 0.020401940650530453 5 0.05033226017630864 6 0.038617959088504066 7 0.013788124725358493 8 0.04495152857616874 9 0.02600686940067618 10-11 0.02076626101928992 12-13 0.032732783900851055 14-15 0.017487377700454672 16-17 0.016338367306674796 18-19 0.015946022294164596 20-21 0.013535902931601933 22-23 0.007398505950192362 24-25 0.002045798993803191 26-27 0.0 28-29 0.0022419715000582914 30-31 0.0 32-33 1.961725062551005E-4 34-35 8.407393125218593E-5 36-37 0.0 38-39 1.961725062551005E-4 40-41 2.8024643750728642E-5 42-43 1.401232187536432E-4 44-45 1.1209857500291457E-4 46-47 0.0 48-49 5.044435875131156E-4 50-51 2.522217937565578E-4 52-53 7.84690025020402E-4 54-55 0.0019337004188002764 56-57 1.961725062551005E-4 58-59 0.0014292568312871607 60-61 0.003699252975096181 62-63 0.0026062918688177636 64-65 0.0016814786250437185 66-67 0.012414917181572787 68-69 0.015329480131648567 70-71 0.02721192908195751 72-73 0.018412190944228718 74-75 0.019196880969249118 76-77 0.03155574886332045 78-79 0.021298729250553768 80-81 0.04652090862620955 82-83 0.03519895255091517 84-85 0.020542063869284092 86-87 0.01443269153162525 88-89 0.014740962612883267 90-91 0.024633661856890476 92-93 0.03203216780708284 94-95 0.012302818606569873 96-97 0.01897268381924329 98-99 0.027520200163215523 100-101 0.0384498112259997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1784144.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.334769601098365 #Duplication Level Percentage of deduplicated Percentage of total 1 76.14663204134024 42.89702971948517 2 13.457082560524498 15.162032911002127 3 4.50622789294617 7.615719303574964 4 1.9823919803566046 4.4671038188981775 5 1.0722886459515253 3.020356690777646 6 0.627591275231631 2.1213125936300066 7 0.4257473420735224 1.6789064896794326 8 0.29360296790866824 1.3232044441066801 9 0.2207058718016092 1.1190072993797884 >10 1.14879617935293 11.734418954246664 >50 0.0824127303034489 3.196229799227985 >100 0.033727029255429954 3.235612448482561 >500 0.001995344966975556 0.7035010807244036 >1k 4.98836241743889E-4 0.39679776543215545 >5k 1.9953449669755558E-4 0.713730144932428 >10k+ 9.976724834877779E-5 0.615036536419841 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10943 0.613347353128447 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7600 0.4259745850110753 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5099 0.28579531696993066 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1956 0.10963240635285043 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.6049287501457284E-5 2 0.0 0.0 0.0 0.0 5.6049287501457284E-5 3 0.0 0.0 0.0 0.0 5.6049287501457284E-5 4 0.0 0.0 0.0 5.6049287501457284E-5 5.6049287501457284E-5 5 0.0 0.0 0.0 5.6049287501457284E-5 1.1209857500291457E-4 6 0.0 0.0 0.0 5.6049287501457284E-5 1.1209857500291457E-4 7 5.6049287501457284E-5 0.0 0.0 5.6049287501457284E-5 1.1209857500291457E-4 8 5.6049287501457284E-5 0.0 0.0 5.6049287501457284E-5 1.1209857500291457E-4 9 5.6049287501457284E-5 0.0 0.0 5.6049287501457284E-5 1.1209857500291457E-4 10-11 5.6049287501457284E-5 0.0 0.0 5.6049287501457284E-5 1.1209857500291457E-4 12-13 5.6049287501457284E-5 0.0 0.0 5.6049287501457284E-5 1.1209857500291457E-4 14-15 5.6049287501457284E-5 0.0 0.0 5.6049287501457284E-5 1.1209857500291457E-4 16-17 5.6049287501457284E-5 0.0 0.0 5.6049287501457284E-5 1.1209857500291457E-4 18-19 5.6049287501457284E-5 0.0 0.0 5.6049287501457284E-5 1.1209857500291457E-4 20-21 5.6049287501457284E-5 0.0 0.0 1.1209857500291457E-4 1.1209857500291457E-4 22-23 1.1209857500291457E-4 0.0 0.0 2.802464375072864E-4 1.6814786250437185E-4 24-25 1.1209857500291457E-4 0.0 0.0 3.362957250087437E-4 2.2419715000582914E-4 26-27 1.1209857500291457E-4 0.0 0.0 4.2036965626092963E-4 2.2419715000582914E-4 28-29 1.6814786250437185E-4 0.0 0.0 0.0012611089687827887 2.2419715000582914E-4 30-31 1.6814786250437185E-4 0.0 0.0 0.003531105112591809 2.2419715000582914E-4 32-33 1.6814786250437185E-4 0.0 0.0 0.006697889856424145 2.2419715000582914E-4 34-35 1.6814786250437185E-4 0.0 0.0 0.009836649956505752 2.2419715000582914E-4 36-37 1.6814786250437185E-4 0.0 0.0 0.013648001506604848 2.2419715000582914E-4 38-39 1.961725062551005E-4 0.0 0.0 0.01706700804419374 2.2419715000582914E-4 40-41 2.2419715000582914E-4 0.0 0.0 0.01995354635051879 2.2419715000582914E-4 42-43 2.522217937565578E-4 0.0 0.0 0.0232884789568555 2.522217937565578E-4 44-45 3.362957250087437E-4 0.0 0.0 0.026847608713198037 3.0827108125801506E-4 46-47 3.6432036875947235E-4 0.0 0.0 0.032312414244590124 4.483943000116583E-4 48-49 3.92345012510201E-4 0.0 0.0 0.03889820552601135 5.044435875131156E-4 50-51 3.92345012510201E-4 0.0 0.0 0.044391035701154165 5.044435875131156E-4 52-53 3.92345012510201E-4 0.0 0.0 0.05632953393896457 5.044435875131156E-4 54-55 3.92345012510201E-4 0.0 0.0 0.08146763938336815 5.044435875131156E-4 56-57 3.92345012510201E-4 0.0 0.0 0.12664336510954272 5.044435875131156E-4 58-59 3.92345012510201E-4 0.0 0.0 0.17509797415455255 5.324682312638441E-4 60-61 3.92345012510201E-4 0.0 0.0 0.22178703064326646 5.604928750145728E-4 62-63 4.483943000116583E-4 0.0 0.0 0.2751739769884045 5.604928750145728E-4 64-65 4.483943000116583E-4 0.0 0.0 0.3366880700212539 5.604928750145728E-4 66-67 4.483943000116583E-4 0.0 0.0 0.4300661829986817 5.604928750145728E-4 68-69 4.483943000116583E-4 0.0 0.0 0.5531223937081312 5.604928750145728E-4 70-71 4.764189437623869E-4 0.0 0.0 0.6605128285609233 5.604928750145728E-4 72-73 5.044435875131156E-4 0.0 0.0 0.7670064748136922 6.165421625160301E-4 74-75 5.044435875131156E-4 0.0 0.0 0.8818794895479289 6.165421625160301E-4 76-77 5.044435875131156E-4 0.0 0.0 1.011801737976307 6.165421625160301E-4 78-79 5.044435875131156E-4 0.0 0.0 1.1226672286541892 6.165421625160301E-4 80-81 7.286407375189447E-4 0.0 0.0 1.2422483835385485 6.165421625160301E-4 82-83 7.84690025020402E-4 0.0 0.0 1.3817550601296755 6.725914500174874E-4 84-85 8.407393125218593E-4 0.0 0.0 1.5830560761911594 7.286407375189447E-4 86-87 9.248132437740452E-4 0.0 0.0 1.8208732030598427 7.84690025020402E-4 88-89 9.528378875247738E-4 0.0 0.0 2.1175140571613054 8.407393125218593E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7290 0.0 37.21973 1 GTATCAA 11865 0.0 36.244778 1 ATCAACG 14595 0.0 29.074482 3 TATCAAC 14760 0.0 28.878239 2 TCAACGC 14775 0.0 28.720274 4 CAACGCA 14930 0.0 28.485762 5 AACGCAG 15305 0.0 27.879389 6 GTACATG 10510 0.0 25.228773 1 ACGCAGA 16950 0.0 24.920694 7 CGCAGAG 17200 0.0 24.44866 8 TACATGG 10730 0.0 24.224354 2 GAGTACT 8795 0.0 23.39236 12-13 ACATGGG 10765 0.0 23.218616 3 GCAGAGT 17705 0.0 23.134695 9 AGTACTT 9170 0.0 21.606714 12-13 ATACCGT 110 0.0028723483 21.598202 6 GTACTTT 9685 0.0 21.287308 14-15 AGAGTAC 14715 0.0 21.279995 10-11 CATGGGA 7020 0.0 20.713284 4 CATGGGG 3935 0.0 20.166769 4 >>END_MODULE