FastQCFastQC Report
Thu 26 May 2016
SRR937125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784144
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113000.6333569487664673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87070.4880211462751885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59010.3307468455460994No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21080.11815189805307194No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19650.11013684994036356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71400.037.397391
GTATCAA118900.036.722891
ATCAACG151150.028.7190993
TATCAAC152500.028.5590922
TCAACGC153400.028.359784
CAACGCA153800.028.2614785
AACGCAG157400.027.6452716
GTACATG104150.024.816631
ACGCAGA177900.024.3261017
TACATGG105300.024.0405122
CGCAGAG178450.024.0381668
ACGACCG1000.001653193423.7519975
ACATGGG103300.023.4938033
GAGTACT95600.023.37407912-13
GGACCGA1452.3337616E-522.9329626
AGTACTT98550.022.62620712-13
GCAGAGT188000.022.5138599
AGAGTAC153700.021.7739510-11
TATGACG2205.6521458E-821.5878834
GTACTTT103750.021.40091514-15
Produced by FastQC (version 0.11.5)