##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937125_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1784144 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0405516595073 31.0 31.0 34.0 30.0 34.0 2 32.156406657758566 33.0 31.0 34.0 30.0 34.0 3 32.257057165789305 34.0 31.0 34.0 30.0 34.0 4 35.9469644826875 37.0 35.0 37.0 35.0 37.0 5 35.84316960962792 37.0 35.0 37.0 35.0 37.0 6 35.8836433606256 37.0 35.0 37.0 35.0 37.0 7 35.81728212520962 37.0 35.0 37.0 35.0 37.0 8 35.785294236339666 37.0 35.0 37.0 33.0 37.0 9 37.43103303320808 39.0 37.0 39.0 34.0 39.0 10-11 37.338636623501245 39.0 37.0 39.0 34.0 39.0 12-13 37.23615722721933 39.0 37.0 39.0 33.0 39.0 14-15 38.37130915441803 40.0 38.0 41.0 33.5 41.0 16-17 38.29055418172524 40.0 38.0 41.0 33.0 41.0 18-19 38.47132210180344 40.0 38.0 41.0 34.0 41.0 20-21 38.51200912034006 40.0 38.0 41.0 34.0 41.0 22-23 38.424883865876296 40.0 38.0 41.0 34.0 41.0 24-25 38.31440931897873 40.0 38.0 41.0 34.0 41.0 26-27 38.11897722381153 40.0 37.5 41.0 33.0 41.0 28-29 38.022683707144715 40.0 37.0 41.0 33.0 41.0 30-31 37.97412400568564 40.0 37.0 41.0 33.0 41.0 32-33 37.778517036741434 40.0 37.0 41.0 33.0 41.0 34-35 37.61321227434557 40.0 36.5 41.0 33.0 41.0 36-37 37.432239774367986 40.0 36.0 41.0 32.5 41.0 38-39 37.269178664950815 40.0 36.0 41.0 32.0 41.0 40-41 37.06056910204558 39.5 36.0 41.0 31.0 41.0 42-43 36.77554334179304 39.0 35.5 41.0 31.0 41.0 44-45 36.45835622012573 39.0 35.0 41.0 30.0 41.0 46-47 36.04641777793721 39.0 35.0 40.5 29.5 41.0 48-49 35.63486327336807 39.0 35.0 40.0 28.0 41.0 50-51 35.327842371467774 39.0 35.0 40.0 27.0 41.0 52-53 35.078441538351164 38.0 34.5 40.0 26.5 41.0 54-55 34.78279275663848 38.0 34.0 40.0 26.0 41.0 56-57 34.47959497663866 38.0 34.0 40.0 25.0 41.0 58-59 34.118666430512334 38.0 33.5 40.0 23.5 41.0 60-61 33.778075368355914 37.0 33.0 40.0 23.0 41.0 62-63 33.39597448412236 36.5 33.0 39.5 22.0 41.0 64-65 33.004829767104 36.0 32.0 39.0 22.0 40.0 66-67 32.60228210278991 36.0 32.0 39.0 20.5 40.0 68-69 32.28565070980818 35.0 31.0 38.5 20.0 40.0 70-71 31.81206393654324 35.0 31.0 38.0 18.0 40.0 72-73 31.31201181070586 35.0 30.5 37.0 16.0 39.0 74-75 30.79389387852102 34.0 30.0 36.5 12.5 39.0 76-77 29.383452793048093 33.0 28.5 35.0 11.0 37.5 78-79 30.136155489691415 34.0 30.0 36.0 9.0 38.0 80-81 29.906540615555695 34.0 30.0 35.0 7.0 37.0 82-83 29.569952033019756 34.0 29.0 35.0 4.5 37.0 84-85 29.191269314584474 34.0 29.0 35.0 2.0 36.0 86-87 28.811033470392523 34.0 29.0 35.0 2.0 36.0 88-89 28.39563342420791 33.5 29.0 35.0 2.0 35.5 90-91 27.898141069330727 33.0 27.0 35.0 2.0 35.0 92-93 27.372422853760682 33.0 26.5 35.0 2.0 35.0 94-95 26.756753658897487 32.5 25.5 34.0 2.0 35.0 96-97 26.04832625617663 32.0 24.5 34.0 2.0 35.0 98-99 25.15063554287098 32.0 21.0 34.0 2.0 35.0 100-101 21.62627512129066 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1916420134496093 1101 2 0.17926370763067467 1101 3 0.19093264796588016 1101 4 0.12428055615323075 1101 5 0.2492493414112147 1101 6 0.2586527076067071 1101 7 0.2586180747777931 1101 8 0.26871074902789616 1101 9 0.22387714002502435 1101 10-11 0.23556178046123222 1101 12-13 0.3079498764770392 1101 14-15 0.3830690813522395 1101 16-17 0.4565843216311407 1101 18-19 0.36886469744759864 1101 20-21 0.3844074709572496 1101 22-23 0.379281213955899 1101 24-25 0.419905009513748 1101 26-27 0.3736741346485246 1101 28-29 0.2884411925935808 1101 30-31 0.3030530031388565 1101 32-33 0.4026513417713957 1101 34-35 0.4432674720357639 1101 36-37 0.33044919651749183 1101 38-39 0.344458648994852 1101 40-41 0.37249357759538526 1101 42-43 0.4058591359450787 1101 44-45 0.3373860442979577 1101 46-47 0.33722298194162903 1101 48-49 0.42578400454442544 1101 50-51 0.1870860839128028 1101 52-53 0.19337996942472557 1101 54-55 0.11125809778484097 1101 56-57 0.1534381860770324 1101 58-59 0.1647966376871608 1101 60-61 0.172310676537478 1101 62-63 0.09990510665471675 1101 64-65 0.15168185612279927 1101 66-67 -0.030010074720181024 1101 68-69 -0.03655494528062064 1101 70-71 -0.057413450042641756 1101 72-73 -0.16509189602784957 1101 74-75 -0.08407261231453589 1101 76-77 -0.08911955958864937 1101 78-79 0.0501377359826094 1101 80-81 0.13142164225252628 1101 82-83 0.17673607893252807 1101 84-85 0.03456684523977671 1101 86-87 -0.32826467806544457 1101 88-89 -0.1831636314061562 1101 90-91 0.1639793153983291 1101 92-93 0.11769863913207956 1101 94-95 0.10175725521411394 1101 96-97 0.23560955296873942 1101 98-99 0.35373991742604005 1101 100-101 0.6867235776318239 1102 1 0.24121875181224794 1102 2 0.2573680934808422 1102 3 0.243365390165593 1102 4 0.0999518883528765 1102 5 0.22204411402098145 1102 6 0.09381488003833027 1102 7 0.0771780508583646 1102 8 0.12949597878515107 1102 9 0.29216023634430144 1102 10-11 0.22681406183834696 1102 12-13 0.3314192515198613 1102 14-15 0.3596401949134318 1102 16-17 0.39847351989448754 1102 18-19 0.3147501209272505 1102 20-21 0.32631006160500675 1102 22-23 0.3333693171582368 1102 24-25 0.2973986403448876 1102 26-27 0.31141667695911934 1102 28-29 0.3177431067702017 1102 30-31 0.37818770560990345 1102 32-33 0.23992733078340933 1102 34-35 0.4145323983153588 1102 36-37 0.3495928313974872 1102 38-39 0.4642569534566974 1102 40-41 0.3748347532598615 1102 42-43 0.4607694692597235 1102 44-45 0.2849501875902476 1102 46-47 0.3513728146925601 1102 48-49 0.45175993562659045 1102 50-51 0.5324949876682723 1102 52-53 0.4936465713096254 1102 54-55 0.6527695062276493 1102 56-57 0.7682408197049924 1102 58-59 0.8523632102956853 1102 60-61 0.730504947088555 1102 62-63 0.6865248688308867 1102 64-65 0.6205607578227017 1102 66-67 0.40707624075456295 1102 68-69 0.31886959330501696 1102 70-71 0.2609322478874567 1102 72-73 0.30758795123668037 1102 74-75 0.5123883722437341 1102 76-77 0.3626376458256111 1102 78-79 0.31290566153341004 1102 80-81 0.38402723964630425 1102 82-83 0.22487142299222285 1102 84-85 0.29814516577248185 1102 86-87 0.25528511357396866 1102 88-89 0.31076619249956394 1102 90-91 0.5649781381143058 1102 92-93 0.3354342049447503 1102 94-95 0.3967575355198356 1102 96-97 0.706633104878243 1102 98-99 1.0598007499963167 1102 100-101 1.4026654982866198 1103 1 0.23336514635101224 1103 2 0.2914084344578569 1103 3 0.18911954037372425 1103 4 0.17631426881589363 1103 5 0.14325989827658248 1103 6 0.20188573215482108 1103 7 0.0450439013506454 1103 8 0.14810444089861363 1103 9 0.2501201706993399 1103 10-11 0.29318116400465755 1103 12-13 0.2781295164545128 1103 14-15 0.35075801892069336 1103 16-17 0.37915317949711635 1103 18-19 0.3925232762930264 1103 20-21 0.3008198075145785 1103 22-23 0.33571960233221176 1103 24-25 0.2568973548581468 1103 26-27 0.31504671278851504 1103 28-29 0.3710684659396648 1103 30-31 0.25046019740126724 1103 32-33 0.3124124199825502 1103 34-35 0.5055867027335879 1103 36-37 0.29164962225743807 1103 38-39 0.29875008283499227 1103 40-41 0.3826228465974282 1103 42-43 0.4447171875660487 1103 44-45 0.45243329029272417 1103 46-47 0.3466499038047388 1103 48-49 0.2750414348821053 1103 50-51 0.355064004841509 1103 52-53 0.3551379412194535 1103 54-55 0.307600013985855 1103 56-57 0.20105979732944235 1103 58-59 0.3756121648187971 1103 60-61 0.3544973585505744 1103 62-63 0.5386084562627005 1103 64-65 0.42956936919512145 1103 66-67 0.33351397525476045 1103 68-69 0.21493174106413448 1103 70-71 0.16787816325183158 1103 72-73 0.1588739210153669 1103 74-75 0.36057655446042247 1103 76-77 0.22658919221362694 1103 78-79 0.18591659873716893 1103 80-81 0.35412261563759273 1103 82-83 0.2808854963247427 1103 84-85 0.1128469920210442 1103 86-87 0.03630917976959225 1103 88-89 -0.010912222070814437 1103 90-91 0.137879848192064 1103 92-93 0.23275949890398806 1103 94-95 -0.023288691283742224 1103 96-97 -0.14123929078841257 1103 98-99 -0.2024125816302771 1103 100-101 0.02370261860310663 1104 1 0.2839688521910517 1104 2 0.2938614957617105 1104 3 0.25482399347391294 1104 4 0.1913404844908584 1104 5 0.1315519583944038 1104 6 0.0487719321457476 1104 7 0.18850748132951622 1104 8 0.25304167234186536 1104 9 0.2411277729210326 1104 10-11 0.27250999087750216 1104 12-13 0.2318891488517778 1104 14-15 0.21026112008274112 1104 16-17 0.28817441775839114 1104 18-19 0.29023515662770905 1104 20-21 0.27753678840208096 1104 22-23 0.27256704589883185 1104 24-25 0.36815842927955345 1104 26-27 0.1884240305587923 1104 28-29 0.04287511156466195 1104 30-31 0.31882024328680103 1104 32-33 0.2969292932853307 1104 34-35 0.2610194071931744 1104 36-37 0.3004313872949922 1104 38-39 0.3084294673985255 1104 40-41 0.315772875437915 1104 42-43 0.16730900723849373 1104 44-45 0.14130604867428787 1104 46-47 0.28690797751972497 1104 48-49 0.18194824447659386 1104 50-51 0.29475499040830755 1104 52-53 0.33169775767732546 1104 54-55 0.14635579018370493 1104 56-57 0.05763186289130573 1104 58-59 0.20662475741619346 1104 60-61 0.3206276138374591 1104 62-63 0.41421037982570397 1104 64-65 0.2535954358069148 1104 66-67 0.37703718050178026 1104 68-69 0.33448850496751703 1104 70-71 0.13072092753111164 1104 72-73 0.12311010188732041 1104 74-75 0.3197948153468957 1104 76-77 0.27490672269056304 1104 78-79 0.2503410528743224 1104 80-81 0.5431736017228666 1104 82-83 0.46340362694297 1104 84-85 0.5176426134524412 1104 86-87 0.3775617640712987 1104 88-89 0.3129485788520796 1104 90-91 0.4860752116793634 1104 92-93 0.5972335327478078 1104 94-95 0.46640974744157404 1104 96-97 0.49331614450844796 1104 98-99 0.5646389488596206 1104 100-101 0.7282043263485569 1105 1 0.2600276242520536 1105 2 0.26929641035238205 1105 3 0.2711129231844822 1105 4 0.20195303663708586 1105 5 0.13844982433312225 1105 6 0.22047726160722192 1105 7 0.20454781667243793 1105 8 0.13795085030083953 1105 9 0.23496676189247978 1105 10-11 0.25392512059298866 1105 12-13 0.26831394333594005 1105 14-15 0.3465026913107465 1105 16-17 0.2191945028440685 1105 18-19 0.29420080875406995 1105 20-21 0.33360807109321655 1105 22-23 0.3344172408682411 1105 24-25 0.2653313170365479 1105 26-27 0.2779468800361542 1105 28-29 0.11872080672491592 1105 30-31 0.2507556076351847 1105 32-33 0.2366331618712607 1105 34-35 0.28474454496635104 1105 36-37 0.24695234957560785 1105 38-39 0.2623390672146044 1105 40-41 0.3594561511471994 1105 42-43 0.2503395260334571 1105 44-45 0.31792704577485864 1105 46-47 0.168059362840431 1105 48-49 0.17269570719580685 1105 50-51 0.3816157039048278 1105 52-53 0.24911378802425332 1105 54-55 0.19042242017038546 1105 56-57 0.33494766869418413 1105 58-59 0.3251586533000719 1105 60-61 0.17339725083946433 1105 62-63 0.217045611144016 1105 64-65 0.06460763618234466 1105 66-67 0.20073048368757895 1105 68-69 0.11269224744273032 1105 70-71 8.392986269782909E-4 1105 72-73 0.2668761865846214 1105 74-75 0.23905870259274664 1105 76-77 0.1017286599298366 1105 78-79 0.28521931696580083 1105 80-81 0.4597505214517206 1105 82-83 0.46445966474493616 1105 84-85 0.4573166283937731 1105 86-87 0.2549866054284706 1105 88-89 0.3830403327732306 1105 90-91 0.420463596993379 1105 92-93 0.38232314390269195 1105 94-95 0.29066734031151853 1105 96-97 0.5115903415849132 1105 98-99 0.3994715675655236 1105 100-101 0.7708609993914415 1106 1 0.13972751760312008 1106 2 0.07028118589307297 1106 3 0.01783141749150019 1106 4 0.08327101453873809 1106 5 0.12696617357168805 1106 6 0.13805857602296356 1106 7 0.14164599414144874 1106 8 0.15541245452534724 1106 9 0.1683899768181476 1106 10-11 0.060933186188556476 1106 12-13 0.15516352515984977 1106 14-15 0.23582662557700473 1106 16-17 0.05975839289636298 1106 18-19 -0.0915328992178317 1106 20-21 0.1497196163801604 1106 22-23 0.04155068372902093 1106 24-25 0.14490221698947892 1106 26-27 0.07840731630369646 1106 28-29 0.21620770229099406 1106 30-31 0.16274864738890216 1106 32-33 0.07719057526757922 1106 34-35 0.011767798320178713 1106 36-37 0.1236621867524832 1106 38-39 0.2590332740617285 1106 40-41 0.24241515175600625 1106 42-43 0.13204772245747876 1106 44-45 -0.07591489575388977 1106 46-47 0.08882530297022839 1106 48-49 0.07132713581658123 1106 50-51 -0.10533059598161287 1106 52-53 0.06271481953965719 1106 54-55 -0.10270158404376417 1106 56-57 -0.09677110626792995 1106 58-59 0.07638678918895181 1106 60-61 -0.026679605478079793 1106 62-63 0.18165581360428007 1106 64-65 -0.0445029678305886 1106 66-67 0.1542550798680793 1106 68-69 0.014959778671006063 1106 70-71 -0.18508795341424644 1106 72-73 -0.07402826762757897 1106 74-75 -0.08510143704395645 1106 76-77 -0.04673815368495937 1106 78-79 0.26142894041302256 1106 80-81 0.4732818749432859 1106 82-83 0.3266067409665432 1106 84-85 0.32562165514235986 1106 86-87 0.03356269102395615 1106 88-89 0.11905804210954685 1106 90-91 0.21355602750965375 1106 92-93 0.13961936671692854 1106 94-95 0.21151315919831148 1106 96-97 0.07087090384500527 1106 98-99 -0.07402541647167737 1106 100-101 0.1631358600353927 1107 1 0.05669127001205254 1107 2 0.06993074725445325 1107 3 0.06438072388596083 1107 4 -0.012403563740832624 1107 5 -0.053849699098336146 1107 6 -0.047229816730201435 1107 7 -0.009061403548450642 1107 8 -0.007585786841545428 1107 9 0.01557243406383435 1107 10-11 0.050189129289769596 1107 12-13 0.03646987775907462 1107 14-15 0.016514156133055735 1107 16-17 0.07034614772239678 1107 18-19 0.08044647266518012 1107 20-21 -2.1780912479840708E-4 1107 22-23 -0.05648143963396279 1107 24-25 0.06420634137855785 1107 26-27 -0.053696109784070245 1107 28-29 0.12357142164169233 1107 30-31 0.18475450341711763 1107 32-33 0.1481369581558667 1107 34-35 0.06412174778510149 1107 36-37 0.19465740565319578 1107 38-39 0.06622346457047712 1107 40-41 0.11542837128891392 1107 42-43 0.11972454504698504 1107 44-45 -0.027516797460414466 1107 46-47 -0.09868754331601082 1107 48-49 0.029291403955276962 1107 50-51 0.19698605564964566 1107 52-53 0.17010802832863448 1107 54-55 0.338800252136636 1107 56-57 0.3541928414850517 1107 58-59 0.29017797176640414 1107 60-61 0.19763868940449925 1107 62-63 0.19260957019378822 1107 64-65 0.05659804275287428 1107 66-67 0.16304636709413955 1107 68-69 0.08177079779272134 1107 70-71 0.02790427736649903 1107 72-73 -0.04489087797147917 1107 74-75 0.1496134277259067 1107 76-77 0.12217608759392107 1107 78-79 0.4377873194652686 1107 80-81 0.6185024661381 1107 82-83 0.41825771481565965 1107 84-85 0.32677641217246745 1107 86-87 0.4745451749952778 1107 88-89 0.5586560787945167 1107 90-91 0.6031127145819042 1107 92-93 0.5807280998302673 1107 94-95 0.566752753505213 1107 96-97 0.5996245819748971 1107 98-99 0.41968587360940646 1107 100-101 0.47586571799773125 1108 1 0.16478117766303058 1108 2 0.10615241458278746 1108 3 0.0724598717074656 1108 4 0.1353140538103048 1108 5 0.12108843188217833 1108 6 0.1101104276292375 1108 7 0.1324470503382642 1108 8 0.09751734055853944 1108 9 0.21486488251868252 1108 10-11 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2316 66-67 -1.1786524275941055 2316 68-69 -1.47325762532369 2316 70-71 -1.1990365290910496 2316 72-73 -0.9470113708804107 2316 74-75 -1.0416544760544006 2316 76-77 -1.0541294203940943 2316 78-79 -0.9525585035730337 2316 80-81 -1.057479949767366 2316 82-83 -0.9738993951959607 2316 84-85 -1.1939427916729848 2316 86-87 -1.1415786708818239 2316 88-89 -1.1032553797698306 2316 90-91 -1.0911792673133434 2316 92-93 -1.227103716028406 2316 94-95 -1.331547019707049 2316 96-97 -0.8904005841298002 2316 98-99 -1.2725931967782849 2316 100-101 -1.1201461168261169 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 16.0 5 64.0 6 124.0 7 280.0 8 639.0 9 1247.0 10 2103.0 11 3442.0 12 4879.0 13 7119.0 14 9118.0 15 12518.0 16 16402.0 17 21922.0 18 19203.0 19 11808.0 20 10413.0 21 10911.0 22 11482.0 23 12844.0 24 14906.0 25 17728.0 26 21529.0 27 26397.0 28 32651.0 29 40261.0 30 49872.0 31 60946.0 32 75790.0 33 97754.0 34 129290.0 35 178482.0 36 263185.0 37 368816.0 38 236675.0 39 13318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83730455730678 19.949016290231523 15.261587244990348 21.95209190747135 2 15.349181864246415 21.57196859291412 39.88423009143211 23.194619451407355 3 18.867174735629078 26.39998744493849 30.489014040930623 24.24382377850181 4 12.78269954454196 17.839539318146915 37.55639978521841 31.82136135209272 5 13.180613885770368 38.37102743446013 33.801943005689566 14.646415674079934 6 30.346317337614003 37.24721771336843 17.778273502587236 14.628191446430334 7 27.215067842057593 34.08648629258625 21.134448788886996 17.563997076469164 8 26.383352464823467 34.59507752737447 19.98583074011963 19.03573926768243 9 26.959274004381932 16.405691696237355 20.534452675598313 36.100581623782396 10-11 24.45742015806665 27.424491193885864 27.27532623178811 20.842762416259372 12-13 25.957882323399907 24.979542010061966 28.050538521554312 21.012037144983815 14-15 22.807043601861736 25.6172988278973 27.21660919746388 24.359048372777085 16-17 21.291975311409843 29.136717664045054 27.470428395914233 22.100878628630873 18-19 21.2158017912837 27.171305831186405 30.819451057498586 20.79344132003131 20-21 22.41814710302862 26.078081171752853 30.837850706548654 20.665921018669877 22-23 21.950174373438287 26.294740995750242 30.702280474995263 21.052804155816204 24-25 21.707908866458684 26.33353391778429 30.91470713782618 21.04385007793084 26-27 21.602663828926175 26.619679902199966 30.708890481396665 21.06876578747719 28-29 21.286828348808122 26.741926145307666 30.83462971064186 21.136615795242353 30-31 21.575969584477008 26.385454985709796 30.801350156858764 21.237225272954433 32-33 21.376961776347336 26.617044049111428 30.640579059457178 21.365415115084062 34-35 21.79008532943529 26.546736137890214 30.51101816893704 21.15216036373746 36-37 21.852717677819257 26.320245762951757 30.501618417759463 21.325418141469527 38-39 21.65636640348443 26.61481500166766 30.49912132448772 21.229697270360187 40-41 21.806518764807794 26.499612412007995 30.370279651882647 21.32358917130156 42-43 21.634856827700006 26.696387735519107 30.064473495412926 21.60428194136796 44-45 21.82430341945493 26.717097947250895 29.843919549094693 21.61467908419948 46-47 22.124272511813146 27.043209442332156 29.199491854738614 21.633026191116087 48-49 22.140969614863014 27.197022330002667 28.863343813994835 21.79866424113948 50-51 22.05099790388475 27.42514144363737 28.681661499948568 21.84219915252931 52-53 22.325608246867965 27.46274964352653 28.353568994430944 21.85807311517456 54-55 22.14381621549816 27.34061267666224 28.411859382615052 22.10371172522455 56-57 22.226815310847627 27.64086169830246 28.168976585430656 21.96334640541926 58-59 22.45934873908367 27.333520538478222 28.19359926552808 22.013531456910027 60-61 22.304905954377748 27.25390706557761 28.68229633023795 21.758890649806695 62-63 22.205197810922908 27.31509027686887 28.710799284432888 21.76891262777534 64-65 22.125826762253148 27.380981406484402 28.5816695798292 21.91152225143325 66-67 22.18418250098089 27.60770136203128 28.13045793397231 22.077658203015528 68-69 22.16249830800407 27.45780799720649 28.392518393361936 21.9871753014275 70-71 22.12973389256046 27.39743034276109 28.392593809079948 22.080241955598503 72-73 22.228895201284203 27.38957729869338 28.171773130419965 22.209754369602454 74-75 22.068488325070266 27.82228559530105 27.97563648888108 22.133589590747604 76-77 22.091966791918143 27.648749204100113 28.1038413939682 22.155442610013544 78-79 22.092692819044586 27.649951684539275 27.934659785169956 22.32269571124618 80-81 21.989477587114823 27.794096339519754 27.972954377748067 22.243471695617362 82-83 22.017966517174585 27.68621181498484 28.014812244542174 22.281009423298396 84-85 22.07048071697651 27.63351170675421 27.84301382601879 22.452993750250492 86-87 22.06345233787923 27.590021319308423 27.86150829272613 22.48501805008622 88-89 22.08023356898362 27.52772903038493 27.702014582928143 22.69002281770331 90-91 21.96009314346296 27.48637360684861 27.841981201476685 22.711552048211747 92-93 21.99374937294754 27.522096067519325 27.80943322304683 22.674721336486297 94-95 21.841790797155387 27.490942435139765 27.78116564582231 22.886101121882536 96-97 21.85682116588359 27.61303684355816 27.724157452157183 22.80598453840107 98-99 21.786167794543427 27.666428732173483 27.48768359700903 23.059719876274062 100-101 21.636054345511003 27.731652224601213 27.552013855414938 23.080279574472858 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1355.0 1 952.0 2 595.0 3 636.5 4 554.0 5 556.5 6 943.5 7 1965.5 8 3163.5 9 3634.0 10 3152.5 11 2566.5 12 2555.5 13 2820.5 14 3022.0 15 3171.0 16 3381.0 17 3524.0 18 3824.5 19 4110.0 20 4364.0 21 4995.0 22 5865.0 23 6900.0 24 8099.0 25 9932.0 26 12162.5 27 14608.0 28 17022.0 29 19335.0 30 22422.0 31 25762.5 32 28943.5 33 32140.0 34 35919.5 35 39031.5 36 42274.0 37 46721.0 38 49462.0 39 50715.5 40 52423.5 41 54934.0 42 57178.5 43 59571.0 44 62477.0 45 64722.5 46 64963.5 47 65975.0 48 70574.5 49 70852.0 50 66518.5 51 63248.5 52 60607.0 53 58734.5 54 56041.5 55 51464.5 56 46241.0 57 41413.5 58 37757.0 59 34027.5 60 29080.0 61 23815.0 62 18833.0 63 14170.5 64 10418.0 65 7762.0 66 5461.5 67 4005.5 68 2894.0 69 1802.5 70 1166.5 71 755.5 72 519.0 73 409.5 74 302.5 75 232.5 76 164.5 77 72.5 78 32.5 79 16.5 80 10.5 81 6.5 82 2.5 83 1.0 84 1.5 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02449353863813683 2 0.004932337300128241 3 1.6814786250437185E-4 4 3.362957250087437E-4 5 5.6049287501457284E-5 6 0.0 7 0.0 8 0.0 9 5.6049287501457284E-5 10-11 0.011938498237810402 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.522217937565578E-4 20-21 7.566653812696733E-4 22-23 0.002354070075061206 24-25 0.010677389269027613 26-27 0.005352706956389171 28-29 3.0827108125801506E-4 30-31 0.0030827108125801505 32-33 0.004399869068864397 34-35 0.0 36-37 0.008323319193966406 38-39 0.011714301087804573 40-41 0.0018496264875480905 42-43 0.0 44-45 0.0 46-47 4.764189437623869E-4 48-49 0.003615179043843995 50-51 0.0072583827314387185 52-53 0.0 54-55 0.003054686168829422 56-57 0.00350308046884108 58-59 2.802464375072864E-4 60-61 0.002045798993803191 62-63 0.005857150543902286 64-65 2.8024643750728642E-5 66-67 0.0024661686500641205 68-69 4.764189437623869E-4 70-71 8.407393125218593E-4 72-73 0.0 74-75 2.8024643750728642E-5 76-77 0.0 78-79 0.0020177743500524622 80-81 0.002662341156319221 82-83 0.012022572169062587 84-85 0.008547516343972236 86-87 0.0045119676438673114 88-89 1.6814786250437185E-4 90-91 8.127146687711307E-4 92-93 1.6814786250437185E-4 94-95 0.0 96-97 0.0026062918688177636 98-99 0.0022139468563075627 100-101 2.2419715000582914E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1784144.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.85197008773871 #Duplication Level Percentage of deduplicated Percentage of total 1 74.15714228349181 39.93508208042783 2 14.358112856729244 15.464253281539195 3 4.945231118106728 7.9893131474771435 4 2.177317264328449 4.690112967605308 5 1.2080382433426178 3.2527619672665526 6 0.7072890585722048 2.2853345535369147 7 0.4675461782204207 1.762479796291385 8 0.3253016594486573 1.4014508187296684 9 0.2457882757536891 1.1912564586424086 >10 1.2739322582370844 12.364199420161427 >50 0.09169326118723206 3.373768957991356 >100 0.039795303428069875 3.6520456533660055 >500 0.001874826102562516 0.6535115753888207 >1k 6.249420341875052E-4 0.5312340683638761 >5k 2.083140113958351E-4 0.8193714782662339 >10k+ 1.0415700569791755E-4 0.6338237749458135 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11300 0.6333569487664673 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8707 0.4880211462751885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5901 0.3307468455460994 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2108 0.11815189805307194 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1965 0.11013684994036356 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.6049287501457284E-5 0.0 0.0 0.0 0.0 6 5.6049287501457284E-5 0.0 0.0 0.0 0.0 7 5.6049287501457284E-5 0.0 0.0 0.0 0.0 8 5.6049287501457284E-5 0.0 0.0 0.0 0.0 9 5.6049287501457284E-5 0.0 0.0 0.0 0.0 10-11 5.6049287501457284E-5 0.0 0.0 0.0 0.0 12-13 1.1209857500291457E-4 0.0 0.0 0.0 1.1209857500291457E-4 14-15 2.2419715000582914E-4 0.0 0.0 0.0 1.1209857500291457E-4 16-17 2.2419715000582914E-4 0.0 0.0 0.0 1.1209857500291457E-4 18-19 3.362957250087437E-4 0.0 0.0 0.0 1.1209857500291457E-4 20-21 3.92345012510201E-4 0.0 0.0 5.6049287501457284E-5 1.6814786250437185E-4 22-23 3.92345012510201E-4 0.0 0.0 2.522217937565578E-4 1.6814786250437185E-4 24-25 4.483943000116583E-4 0.0 0.0 3.362957250087437E-4 1.6814786250437185E-4 26-27 4.764189437623869E-4 0.0 0.0 4.483943000116583E-4 2.2419715000582914E-4 28-29 6.165421625160301E-4 0.0 0.0 0.0013451829000349748 2.2419715000582914E-4 30-31 6.725914500174874E-4 0.0 0.0 0.003643203687594723 2.2419715000582914E-4 32-33 6.725914500174874E-4 0.0 0.0 0.006866037718928518 2.802464375072864E-4 34-35 6.725914500174874E-4 0.0 0.0 0.01011689639401304 2.802464375072864E-4 36-37 6.725914500174874E-4 0.0 0.0 0.014040346519115048 2.802464375072864E-4 38-39 6.725914500174874E-4 0.0 0.0 0.017767624137961957 2.802464375072864E-4 40-41 6.725914500174874E-4 0.0 0.0 0.020682187088037735 2.802464375072864E-4 42-43 6.725914500174874E-4 0.0 0.0 0.023989095050623715 2.802464375072864E-4 44-45 7.286407375189447E-4 0.0 0.0 0.02763229873821844 3.0827108125801506E-4 46-47 8.127146687711307E-4 0.0 0.0 0.03298500569460761 3.6432036875947235E-4 48-49 8.407393125218593E-4 0.0 0.0 0.03968289555103176 3.92345012510201E-4 50-51 8.407393125218593E-4 0.0 0.0 0.04523177501367603 3.92345012510201E-4 52-53 8.407393125218593E-4 0.0 0.0 0.0573384211139908 4.2036965626092963E-4 54-55 8.407393125218593E-4 0.0 0.0 0.08242047727089294 4.483943000116583E-4 56-57 8.407393125218593E-4 0.0 0.0 0.12754015370956606 4.483943000116583E-4 58-59 8.407393125218593E-4 0.0 0.0 0.1765832802733412 4.483943000116583E-4 60-61 8.407393125218593E-4 0.0 0.0 0.22349653391206092 4.483943000116583E-4 62-63 8.407393125218593E-4 0.0 0.0 0.27685545561344826 4.483943000116583E-4 64-65 8.407393125218593E-4 0.0 0.0 0.33862177044005415 4.764189437623869E-4 66-67 8.407393125218593E-4 0.0 0.0 0.43208395734873417 5.324682312638441E-4 68-69 8.407393125218593E-4 0.0 0.0 0.5554764637831924 5.885175187653015E-4 70-71 8.407393125218593E-4 0.0 0.0 0.6630070218547381 6.165421625160301E-4 72-73 8.407393125218593E-4 0.0 0.0 0.7693885695325041 6.725914500174874E-4 74-75 8.407393125218593E-4 0.0 0.0 0.883617017460474 7.286407375189447E-4 76-77 8.407393125218593E-4 0.0 0.0 1.0125584033575765 7.286407375189447E-4 78-79 8.407393125218593E-4 0.0 0.0 1.1226952532979402 7.286407375189447E-4 80-81 0.0010088871750262311 0.0 0.0 1.2415757920885309 7.286407375189447E-4 82-83 0.0010649364625276883 0.0 0.0 1.381194567254661 7.286407375189447E-4 84-85 0.001092961106278417 0.0 0.0 1.5833923719161682 7.286407375189447E-4 86-87 0.0011490103937798744 0.0 0.0 1.821265548072353 7.84690025020402E-4 88-89 0.0011770350375306029 0.0 0.0 2.118803190773839 8.407393125218593E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7140 0.0 37.39739 1 GTATCAA 11890 0.0 36.72289 1 ATCAACG 15115 0.0 28.719099 3 TATCAAC 15250 0.0 28.559092 2 TCAACGC 15340 0.0 28.35978 4 CAACGCA 15380 0.0 28.261478 5 AACGCAG 15740 0.0 27.645271 6 GTACATG 10415 0.0 24.81663 1 ACGCAGA 17790 0.0 24.326101 7 TACATGG 10530 0.0 24.040512 2 CGCAGAG 17845 0.0 24.038166 8 ACGACCG 100 0.0016531934 23.751997 5 ACATGGG 10330 0.0 23.493803 3 GAGTACT 9560 0.0 23.374079 12-13 GGACCGA 145 2.3337616E-5 22.932962 6 AGTACTT 9855 0.0 22.626207 12-13 GCAGAGT 18800 0.0 22.513859 9 AGAGTAC 15370 0.0 21.77395 10-11 TATGACG 220 5.6521458E-8 21.587883 4 GTACTTT 10375 0.0 21.400915 14-15 >>END_MODULE