FastQCFastQC Report
Thu 26 May 2016
SRR937124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804921
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112730.6245702720506882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76270.4225669710751883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52730.2921457504234257No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19660.1089244349198663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115250.038.4318351
GGTATCA67350.038.245281
TATCAAC145100.030.2997232
ATCAACG146300.029.9862173
CAACGCA146550.029.8053345
TCAACGC146950.029.7565484
AACGCAG149400.029.2955116
ACGCAGA166750.025.9901647
CGCAGAG167950.025.6622818
GTACATG103350.025.4290871
TACATGG103350.024.6960852
GCAGAGT175500.023.9348729
GAGTACT83950.023.82930212-13
ACATGGG105150.023.50493
AGTACTT87400.022.99740612-13
AGAGTAC140250.022.66528110-11
CGTAGAC1100.002867604421.6043723
GTACTTT92350.021.5843514-15
CATGGGA68000.020.6194674
TACTTTT103500.019.28203414-15
Produced by FastQC (version 0.11.5)