##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937124_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1804921 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.453023151705807 31.0 31.0 34.0 30.0 34.0 2 31.512449575355376 33.0 31.0 34.0 28.0 34.0 3 31.52198905104434 33.0 31.0 34.0 28.0 34.0 4 35.145615237453605 37.0 35.0 37.0 32.0 37.0 5 35.15615697307528 37.0 35.0 37.0 32.0 37.0 6 35.22080855616395 37.0 35.0 37.0 32.0 37.0 7 35.14677595307496 37.0 35.0 37.0 32.0 37.0 8 35.111313459148626 37.0 35.0 37.0 32.0 37.0 9 36.6718715112739 39.0 37.0 39.0 32.0 39.0 10-11 36.52406559622277 39.0 37.0 39.0 32.0 39.0 12-13 36.41979316546264 39.0 37.0 39.0 32.0 39.0 14-15 37.45668563887284 40.0 37.0 41.0 32.0 41.0 16-17 37.393815297179216 40.0 37.0 41.0 32.0 41.0 18-19 37.37703949369529 40.0 37.0 41.0 32.0 41.0 20-21 37.32008326126185 40.0 37.0 41.0 31.5 41.0 22-23 37.1969507252672 39.5 36.5 41.0 31.0 41.0 24-25 37.1412693408742 39.5 36.5 41.0 31.0 41.0 26-27 37.15119996941694 40.0 36.5 41.0 31.0 41.0 28-29 37.07547948081938 39.5 36.0 41.0 31.0 41.0 30-31 36.877932884597165 39.0 36.0 41.0 30.0 41.0 32-33 36.661560810694766 39.0 35.5 41.0 30.0 41.0 34-35 36.47229407824498 39.0 35.0 41.0 30.0 41.0 36-37 36.267957157127654 39.0 35.0 41.0 30.0 41.0 38-39 36.09288689089439 39.0 35.0 40.5 29.5 41.0 40-41 35.8794792126636 39.0 35.0 40.0 28.5 41.0 42-43 35.621482325265205 38.0 35.0 40.0 27.0 41.0 44-45 35.31329930783674 38.0 35.0 40.0 26.5 41.0 46-47 34.90326224804299 38.0 34.0 40.0 24.5 41.0 48-49 34.59601223543856 38.0 34.0 40.0 24.0 41.0 50-51 33.87515381559636 37.5 33.5 39.5 22.5 40.5 52-53 34.05686869397608 38.0 33.5 39.5 23.0 40.5 54-55 34.43953447269991 38.0 34.0 40.0 23.0 41.0 56-57 34.310636033377634 38.0 34.0 40.0 22.5 41.0 58-59 34.09096492311852 38.0 34.0 40.0 22.0 41.0 60-61 33.79567776096572 38.0 33.0 40.0 21.5 41.0 62-63 33.48445832255262 37.0 33.0 40.0 20.0 41.0 64-65 33.15191966850627 37.0 33.0 40.0 19.0 41.0 66-67 32.774822277540125 36.0 32.0 39.0 18.0 41.0 68-69 32.381966302126244 36.0 32.0 39.0 14.5 41.0 70-71 31.972543119615764 35.0 31.0 39.0 12.5 40.0 72-73 31.59195693329514 35.0 31.0 38.0 9.5 40.0 74-75 31.19683853199115 35.0 31.0 37.0 7.5 39.5 76-77 30.741383971930073 35.0 30.5 37.0 6.5 39.0 78-79 30.277933771062557 35.0 30.0 36.0 2.0 39.0 80-81 29.846386074515173 34.0 29.5 36.0 2.0 37.5 82-83 29.44401084590406 34.0 29.0 35.0 2.0 37.0 84-85 29.07288656955069 34.0 29.0 35.0 2.0 36.5 86-87 28.706806281272144 34.0 29.0 35.0 2.0 36.0 88-89 28.340912427746147 34.0 29.0 35.0 2.0 36.0 90-91 27.962400847460913 34.0 27.5 35.0 2.0 35.5 92-93 27.565205346937624 34.0 26.5 35.0 2.0 35.0 94-95 27.174350013103066 34.0 26.0 35.0 2.0 35.0 96-97 26.653387045748815 33.0 25.0 35.0 2.0 35.0 98-99 26.0522418986759 33.0 24.5 35.0 2.0 35.0 100-101 23.874147123336698 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27130879790256657 1101 2 0.3347783982223902 1101 3 0.28074922374770495 1101 4 0.3854719036326415 1101 5 0.3132997039497596 1101 6 0.2639442881741729 1101 7 0.16290327650653325 1101 8 0.25383305785146604 1101 9 0.3393567056892337 1101 10-11 0.3253456474774339 1101 12-13 0.4088221047858056 1101 14-15 0.5264291352992672 1101 16-17 0.652602688499023 1101 18-19 0.478642180708313 1101 20-21 0.4727685382837308 1101 22-23 0.526544740838709 1101 24-25 0.5310144014331186 1101 26-27 0.5319063834566506 1101 28-29 0.5577943908465315 1101 30-31 0.5684558038786776 1101 32-33 0.5021519254956957 1101 34-35 0.5426518927337796 1101 36-37 0.6006231893669138 1101 38-39 0.4753202475350946 1101 40-41 0.5942247936444218 1101 42-43 0.5758615346455827 1101 44-45 0.8392798663287024 1101 46-47 0.5532511442441788 1101 48-49 0.6982394099557965 1101 50-51 0.7385191750372329 1101 52-53 0.6941921303555318 1101 54-55 0.893194940900834 1101 56-57 0.7519532534259383 1101 58-59 0.8227601876418831 1101 60-61 0.7662388713832016 1101 62-63 0.8290812327616095 1101 64-65 0.602921439568668 1101 66-67 0.7213833009134945 1101 68-69 0.5917371062901893 1101 70-71 0.6382628952839688 1101 72-73 0.5581804846086662 1101 74-75 0.44696556478924876 1101 76-77 0.415738057688376 1101 78-79 0.6791638164496305 1101 80-81 0.7885033499577503 1101 82-83 0.7981035677021815 1101 84-85 0.9315491227674606 1101 86-87 0.7987966123147672 1101 88-89 0.9975684033152987 1101 90-91 0.9007974613797423 1101 92-93 0.8732768997844005 1101 94-95 0.986253608265347 1101 96-97 0.8622363422376935 1101 98-99 0.963347883402772 1101 100-101 1.3339511550422216 1102 1 0.35362512736159957 1102 2 0.1685428072719901 1102 3 0.2822929650783621 1102 4 0.18825506238329837 1102 5 0.2981833553583826 1102 6 0.24216808881510588 1102 7 0.2513246186407443 1102 8 0.10411645575647555 1102 9 0.19398075505069556 1102 10-11 0.2680346989912792 1102 12-13 0.16429724469109885 1102 14-15 0.08250515050948337 1102 16-17 0.28501079343995883 1102 18-19 0.07573376486673311 1102 20-21 0.01403013726972091 1102 22-23 0.1429644806387742 1102 24-25 0.053948406790169656 1102 26-27 0.1942359859821181 1102 28-29 0.19409742332369007 1102 30-31 0.2758633109337296 1102 32-33 0.28326112337200726 1102 34-35 0.3448927689953152 1102 36-37 0.4237930607018754 1102 38-39 0.43461408452748884 1102 40-41 0.17321239349136164 1102 42-43 0.26325570884560534 1102 44-45 0.28386212346746476 1102 46-47 0.3571457728374767 1102 48-49 0.38803170440263557 1102 50-51 0.4907486914671324 1102 52-53 0.6693326335362855 1102 54-55 0.3260445845608473 1102 56-57 0.46253792602363575 1102 58-59 0.1668450037321918 1102 60-61 0.34382143942261933 1102 62-63 0.2797912222388277 1102 64-65 0.5245852363831318 1102 66-67 0.07825480328112633 1102 68-69 0.2689507787443901 1102 70-71 0.3042886282920527 1102 72-73 0.22943712114188486 1102 74-75 0.31617653479929686 1102 76-77 0.28036115043243726 1102 78-79 0.3163059817531142 1102 80-81 0.32991262675499655 1102 82-83 0.34894539101494715 1102 84-85 0.32158595253739364 1102 86-87 0.30909340286338605 1102 88-89 0.33838726781044315 1102 90-91 0.16455959846301127 1102 92-93 0.3312598719946642 1102 94-95 0.33330902032829357 1102 96-97 0.03463434290732792 1102 98-99 0.016511596513211657 1102 100-101 -0.06329139529976757 1103 1 0.2599180036507249 1103 2 0.18065436512942767 1103 3 0.27454343075461196 1103 4 0.20654194167092754 1103 5 0.24599297327669234 1103 6 0.23054094784014012 1103 7 0.16838313483719247 1103 8 0.19864305609267063 1103 9 0.15295013399758517 1103 10-11 0.25202452226146477 1103 12-13 0.23899995015654696 1103 14-15 0.535649884960435 1103 16-17 0.5838406398803713 1103 18-19 0.4595307868102978 1103 20-21 0.5378974649695039 1103 22-23 0.4465927801253926 1103 24-25 0.4512210772731109 1103 26-27 0.586881708575369 1103 28-29 0.5922193850700879 1103 30-31 0.7162059237182419 1103 32-33 0.6264781512217112 1103 34-35 0.6164342709716024 1103 36-37 0.5342872454276915 1103 38-39 0.6886208879439621 1103 40-41 0.6641490274471096 1103 42-43 0.6135101270910326 1103 44-45 0.7422201603581087 1103 46-47 0.7648248878254407 1103 48-49 0.8465939005772753 1103 50-51 0.850013945203898 1103 52-53 0.7190049564657244 1103 54-55 0.7500763186937789 1103 56-57 0.8757225269050082 1103 58-59 0.9147650777860719 1103 60-61 0.7499993163932217 1103 62-63 0.9239898615555262 1103 64-65 0.9155863267470465 1103 66-67 0.8227365396856499 1103 68-69 0.8411439435256369 1103 70-71 0.7787536684992169 1103 72-73 0.6591535129459878 1103 74-75 0.9249133595687304 1103 76-77 0.8187499105978056 1103 78-79 0.8313152384883828 1103 80-81 0.7899647029896038 1103 82-83 0.809210712899457 1103 84-85 0.8229261915088202 1103 86-87 0.8948312157751133 1103 88-89 0.7106216051872103 1103 90-91 0.8645084876547813 1103 92-93 0.8578193195436157 1103 94-95 0.9285892211369813 1103 96-97 1.0813961892568784 1103 98-99 0.9285763027964329 1103 100-101 0.9752325245584821 1104 1 0.27197094394910337 1104 2 0.13631774333337177 1104 3 0.11211576376305032 1104 4 0.34002707553638345 1104 5 0.22802442221991015 1104 6 0.12930272527839293 1104 7 0.11056831611973905 1104 8 0.19908814008237385 1104 9 0.32149262936563616 1104 10-11 0.33943358463249496 1104 12-13 0.10912735064652423 1104 14-15 -0.03785016552997433 1104 16-17 0.33488063267999735 1104 18-19 0.3889690589843724 1104 20-21 0.29167217234461873 1104 22-23 0.24329559134393008 1104 24-25 0.17310944076898238 1104 26-27 0.1521803593836566 1104 28-29 0.22265286724038447 1104 30-31 0.3779755688803874 1104 32-33 0.18503770356930005 1104 34-35 0.2624188011473052 1104 36-37 0.13773167962770572 1104 38-39 0.2426201130258434 1104 40-41 0.2555836028446663 1104 42-43 0.24682348378166807 1104 44-45 0.33342368722340154 1104 46-47 0.12807653644726713 1104 48-49 0.22704796308001818 1104 50-51 0.192844964759054 1104 52-53 0.16184953159985582 1104 54-55 0.2439428308158753 1104 56-57 0.26687205097795896 1104 58-59 0.26481951962550454 1104 60-61 0.2980299706611973 1104 62-63 0.2478013610769665 1104 64-65 0.09179842600680388 1104 66-67 -0.062245744764176436 1104 68-69 -0.051041457280447844 1104 70-71 0.07633375068883552 1104 72-73 0.1375504751791503 1104 74-75 0.1993515439333855 1104 76-77 0.35419198711493394 1104 78-79 0.3890658465686059 1104 80-81 0.263761274909907 1104 82-83 0.30338455798608166 1104 84-85 0.3126231282772558 1104 86-87 0.43017944597628244 1104 88-89 0.31976514504829723 1104 90-91 0.19851328863473228 1104 92-93 0.33202941894387905 1104 94-95 0.460519469725444 1104 96-97 0.558241394423586 1104 98-99 0.34970259030203366 1104 100-101 0.6241378155149881 1105 1 0.18230286142748398 1105 2 0.18069348895143378 1105 3 0.2839225520009663 1105 4 0.20610505899185938 1105 5 0.10329453806655664 1105 6 0.1042704424744727 1105 7 0.20341770236409218 1105 8 0.3137950602919588 1105 9 0.4092608879951385 1105 10-11 0.28651013366084044 1105 12-13 0.3494522052373483 1105 14-15 0.393372030836872 1105 16-17 0.39401633844436645 1105 18-19 0.20714627136698738 1105 20-21 0.29797633207402896 1105 22-23 0.3548975449178684 1105 24-25 0.38443589802895417 1105 26-27 0.5379239508920222 1105 28-29 0.35706035488442467 1105 30-31 0.4387964260844228 1105 32-33 0.417561017106884 1105 34-35 0.05277899748793402 1105 36-37 0.1990233960022394 1105 38-39 0.40932651867702674 1105 40-41 0.34377342245425524 1105 42-43 0.39996578594434595 1105 44-45 0.5531216873282787 1105 46-47 0.5191056860166157 1105 48-49 0.7142506444645846 1105 50-51 0.39131647504803624 1105 52-53 0.5180385165542134 1105 54-55 0.5448294881309081 1105 56-57 0.6801303145017812 1105 58-59 0.6326260806274391 1105 60-61 0.7224614437184371 1105 62-63 0.6150021415451619 1105 64-65 0.725255045784202 1105 66-67 0.7442423340867066 1105 68-69 0.5842010952787859 1105 70-71 0.4540614018116891 1105 72-73 0.522293900630455 1105 74-75 0.8274212780672876 1105 76-77 0.884281089838705 1105 78-79 0.8612624865350433 1105 80-81 0.7077765565295735 1105 82-83 0.7105542901535209 1105 84-85 0.7415197064092887 1105 86-87 0.682000884380038 1105 88-89 0.6914700867753751 1105 90-91 0.807721890172072 1105 92-93 1.0364171217629163 1105 94-95 1.0022940188586702 1105 96-97 0.8443852981629298 1105 98-99 0.7434790556463575 1105 100-101 0.6459220167149731 1106 1 0.29137673222439275 1106 2 0.3276473173479424 1106 3 0.31796971161585574 1106 4 0.31336293502891266 1106 5 0.22631812856128875 1106 6 0.3774180476647828 1106 7 0.20028351534166688 1106 8 0.38766866964412117 1106 9 0.33271320737510024 1106 10-11 0.40036122096555715 1106 12-13 0.4236655577627957 1106 14-15 0.40618172656704843 1106 16-17 0.4716832309502408 1106 18-19 0.6131154576736506 1106 20-21 0.5266566745283967 1106 22-23 0.416302889179228 1106 24-25 0.31741382287877684 1106 26-27 0.20931875692763668 1106 28-29 0.14697588876916967 1106 30-31 0.4656981511528784 1106 32-33 0.46327264546385294 1106 34-35 0.5763880543434468 1106 36-37 0.24817492398872787 1106 38-39 0.12293371088251348 1106 40-41 0.42082523356943113 1106 42-43 0.30084712136000746 1106 44-45 0.09618708760789474 1106 46-47 0.20822802646458172 1106 48-49 0.25343515397585037 1106 50-51 0.3813155666287926 1106 52-53 0.5192453360546807 1106 54-55 0.5535332976137326 1106 56-57 0.536412905448465 1106 58-59 0.5141559767610957 1106 60-61 0.4607496556146842 1106 62-63 0.48699208874715794 1106 64-65 0.20604113498872323 1106 66-67 0.25957226042599757 1106 68-69 0.3698424181734623 1106 70-71 0.4551198649181991 1106 72-73 0.43829943214430855 1106 74-75 0.6163395194913726 1106 76-77 0.5095996342192031 1106 78-79 0.5122084378398348 1106 80-81 0.4792326175843513 1106 82-83 0.36485998644843676 1106 84-85 0.4554244551357449 1106 86-87 0.6279137993174047 1106 88-89 0.7129257962747104 1106 90-91 0.6413572799359599 1106 92-93 0.6534246143936109 1106 94-95 0.7668362500882111 1106 96-97 1.1047527382796751 1106 98-99 1.1291490092157304 1106 100-101 1.4459474412041011 1107 1 0.2758489816506682 1107 2 0.3969417496926724 1107 3 0.3613117664884662 1107 4 0.2044859672011583 1107 5 0.2861240134077363 1107 6 0.32089311819230915 1107 7 0.4049732672893924 1107 8 0.33930446985753093 1107 9 0.5159725294337747 1107 10-11 0.420149857628175 1107 12-13 0.4937699238920388 1107 14-15 0.4468162550923296 1107 16-17 0.6133935783988278 1107 18-19 0.7079972783802404 1107 20-21 0.6840580456128436 1107 22-23 0.5797829295224375 1107 24-25 0.6942172082002784 1107 26-27 0.7280726487318248 1107 28-29 0.6771438393048825 1107 30-31 0.694995214576501 1107 32-33 0.7651177626199441 1107 34-35 0.5566554011927352 1107 36-37 0.5130615683399427 1107 38-39 0.752844108029251 1107 40-41 0.6674383041846639 1107 42-43 0.92470106724749 1107 44-45 0.959771347909296 1107 46-47 0.9517109902287757 1107 48-49 0.9478701297845404 1107 50-51 0.8103932273073227 1107 52-53 0.7284248779546161 1107 54-55 0.8170815998369889 1107 56-57 0.9543666569284497 1107 58-59 0.9795344866934173 1107 60-61 0.9579357167434139 1107 62-63 0.9470371094303616 1107 64-65 1.0126404655253225 1107 66-67 0.9233634989332984 1107 68-69 0.7694604732214785 1107 70-71 0.90491142500181 1107 72-73 0.8193989307776057 1107 74-75 0.9645857592411282 1107 76-77 0.9870650282232702 1107 78-79 1.0085724329869556 1107 80-81 1.1009893012555807 1107 82-83 1.0590511489467893 1107 84-85 1.1257105118793476 1107 86-87 1.2412849422288375 1107 88-89 1.193745669690582 1107 90-91 1.0967511378284662 1107 92-93 1.1255136910310917 1107 94-95 1.2609617817854044 1107 96-97 1.106015071461968 1107 98-99 1.3461311617306002 1107 100-101 1.4239353967096342 1108 1 0.019041154430112783 1108 2 0.0549180153666029 1108 3 0.20987117622142293 1108 4 0.06402068839674513 1108 5 0.0392587991133837 1108 6 0.0842696822252691 1108 7 -0.0018454460037133913 1108 8 -0.0343853711520552 1108 9 -0.0025335251041056495 1108 10-11 -0.15450978460978604 1108 12-13 0.044142200693947586 1108 14-15 0.1275483098721253 1108 16-17 0.1462685767188745 1108 18-19 0.14721872259919877 1108 20-21 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2316 86-87 -3.4829387748803384 2316 88-89 -3.176971379958186 2316 90-91 -3.6187170726727835 2316 92-93 -3.695990654724355 2316 94-95 -4.17334200437962 2316 96-97 -4.550844971400277 2316 98-99 -4.849243287608605 2316 100-101 -4.676940600432886 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4224.0 3 1673.0 4 1702.0 5 1883.0 6 2128.0 7 2484.0 8 3134.0 9 3900.0 10 5087.0 11 6394.0 12 8183.0 13 10072.0 14 11226.0 15 13696.0 16 17184.0 17 21797.0 18 19049.0 19 12053.0 20 11838.0 21 12529.0 22 13815.0 23 15305.0 24 17049.0 25 19909.0 26 22799.0 27 26854.0 28 31925.0 29 38412.0 30 46080.0 31 55847.0 32 69814.0 33 88409.0 34 118168.0 35 162698.0 36 234365.0 37 331007.0 38 291680.0 39 50523.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24067519678275 19.156458497988666 14.609976090106047 21.992890215122536 2 15.063048344360952 20.870102066660053 40.86878307523405 23.198066513744944 3 18.758835633253405 26.49778357568345 30.109732261262646 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30.381813924424208 21.119739961398786 24-25 21.77411423253711 26.419581454147213 30.592185005279127 21.21411930803655 26-27 21.65670011873425 26.679607573062007 30.41117620040435 21.252516107799394 28-29 21.400198472174278 26.742518646244683 30.549643808382925 21.30763907319811 30-31 21.642879127497498 26.48000658233192 30.45514221133663 21.42197207883395 32-33 21.386990025252 26.66309287832636 30.380525346886024 21.569391749535615 34-35 21.77959490338009 26.648129104323793 30.24441989268641 21.327856099609708 36-37 21.85320071054256 26.410723962194528 30.21645395823845 21.519621369024467 38-39 21.66310037764618 26.698244299847524 30.235297507180597 21.4033578153257 40-41 21.833996290983276 26.568117859630284 30.073786937102415 21.524098912284025 42-43 21.672807037227575 26.6899316483813 29.833973134181985 21.80328818020914 44-45 21.806243430978245 26.792494541167162 29.60247064474897 21.798791383105627 46-47 22.07322596775154 27.04213685856637 29.086552246186407 21.79808492749568 48-49 22.050216298633952 27.20575850789401 28.717023323020737 22.0270018704513 50-51 22.047317168305554 27.410210296063347 28.60632594670904 21.93614658892206 52-53 22.22241614839896 27.51970359214998 28.25994751249508 21.997932746955982 54-55 22.022700152906175 27.37267077131592 28.285785765679254 22.31884331009865 56-57 22.101881800488975 27.723679380536492 28.035405830326287 22.139032988648246 58-59 22.387107033408704 27.33486108656277 28.087009905093502 22.191021974935023 60-61 22.247397816528924 27.31560600893633 28.568127993103136 21.8688681814316 62-63 22.12613784675635 27.285854033692203 28.704448382752574 21.883559736798873 64-65 22.012106625400754 27.45961711017273 28.4770811569415 22.051195107485015 66-67 22.06508677192587 27.66136546950327 28.042323497620846 22.231224260950015 68-69 22.017018330609467 27.47795523951237 28.3385163960028 22.166510033875365 70-71 22.089236499647008 27.41728268037128 28.28100924152218 22.212471578459528 72-73 22.097032144678618 27.452807163786797 28.16441537840564 22.285745313128945 74-75 21.94323815582189 27.91352180434796 27.94663047893152 22.19660956089863 76-77 22.006047546360318 27.697518499933636 28.008873207396817 22.287560746309225 78-79 22.01465417656224 27.694803206595182 27.944184118481424 22.346358498361152 80-81 21.909419465225934 27.851686176950096 27.91871797935432 22.32017637846965 82-83 21.97407046772137 27.697459972101978 28.002736599777545 22.325732960399105 84-85 22.05820628108314 27.657389319715094 27.758262771324233 22.526141627877532 86-87 22.04727660465905 27.646666319367856 27.844515800802704 22.461541275170386 88-89 22.10861578507431 27.566565636633328 27.697989672321242 22.62682890597112 90-91 22.006930081447717 27.470099023730388 27.897887303451157 22.625083591370736 92-93 22.003848854849288 27.490434675610377 27.89031317950108 22.61540329003926 94-95 21.89396325808358 27.428162818293217 27.892305940494488 22.78556798312872 96-97 21.912539131364742 27.597686764559608 27.756359444182934 22.733414659892716 98-99 21.830789784569443 27.624654950574207 27.6190294082844 22.925525856571948 100-101 21.84387859540414 27.550340370654165 27.774860807863288 22.830920226078412 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 910.0 1 658.5 2 483.5 3 587.5 4 547.0 5 564.0 6 926.0 7 1857.0 8 3128.5 9 3655.5 10 3116.5 11 2611.0 12 2710.5 13 2899.5 14 2994.0 15 3127.0 16 3267.5 17 3445.0 18 3605.0 19 3747.5 20 4191.0 21 4839.0 22 5632.5 23 6807.0 24 8060.0 25 9816.0 26 12181.0 27 14763.5 28 17042.5 29 19434.0 30 22587.5 31 25905.5 32 29259.5 33 32577.0 34 36388.0 35 39681.0 36 43410.0 37 47797.5 38 50103.0 39 51335.5 40 53048.5 41 55507.0 42 57810.5 43 60270.0 44 63316.0 45 65155.0 46 65354.0 47 66764.0 48 71096.5 49 71511.5 50 67246.5 51 63918.0 52 61477.5 53 59609.0 54 56784.5 55 51987.5 56 47012.0 57 42591.5 58 38664.0 59 34683.0 60 29838.5 61 24514.0 62 19327.5 63 14371.5 64 10391.5 65 7803.5 66 5633.5 67 4068.0 68 2922.0 69 1883.5 70 1259.0 71 882.0 72 599.5 73 434.0 74 319.0 75 240.0 76 172.5 77 88.0 78 40.5 79 26.0 80 18.0 81 10.5 82 13.0 83 13.5 84 8.5 85 5.0 86 6.5 87 3.5 88 1.5 89 5.5 90 5.5 91 2.5 92 2.5 93 2.0 94 0.5 95 0.5 96 1.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03584644424880646 2 0.028366892512193057 3 0.02426699007879015 4 0.04554215946293494 5 0.09302346196869558 6 0.10853660631130115 7 0.05601353189419371 8 0.08393719171088375 9 0.06903349232459481 10-11 0.07086182719354477 12-13 0.06784230445543046 14-15 0.06174785489226398 16-17 0.049780572113682545 18-19 0.059587095501686775 20-21 0.055708809416035385 22-23 0.031774243858872495 24-25 0.009557204996783792 26-27 0.0027702043468938527 28-29 0.007424147649675526 30-31 0.004958665780939997 32-33 0.0062883638674490466 34-35 0.006482278171731616 36-37 0.005706620954601337 38-39 0.003158032955458992 40-41 0.007202531301924019 42-43 0.003462755433617316 44-45 0.0026593961730180986 46-47 0.001579016477729496 48-49 4.986367824408935E-4 50-51 0.0020499512167014514 52-53 0.006122151606635415 54-55 0.012161197082864015 56-57 0.007396445606206588 58-59 0.005097175998284689 60-61 0.002354673694859775 62-63 0.007839678301709604 64-65 0.002299269607921898 66-67 0.02169070003617887 68-69 0.04473880020233573 70-71 0.058257397415177735 72-73 0.003628967694430948 74-75 0.014294254429972283 76-77 0.02146908368842736 78-79 0.02507034933938937 80-81 0.07199761097577123 82-83 0.02725881077343551 84-85 0.010222054040038318 86-87 0.028754721120758196 88-89 0.0463732207670031 90-91 0.041968595855441874 92-93 0.04144225702953204 94-95 0.010942307170230718 96-97 0.014543572821192728 98-99 0.03604035855308903 100-101 0.0719699089323023 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1804921.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.979126573565644 #Duplication Level Percentage of deduplicated Percentage of total 1 75.86049002651379 42.46603973126929 2 13.548188380303689 15.168315043670628 3 4.555177047149261 7.649844974621082 4 2.062311150518237 4.617855077157447 5 1.0559302755303508 2.9555027273386774 6 0.6811632191996441 2.2878553238900583 7 0.4391613275604549 1.7208707279205273 8 0.30106093844678977 1.3482502703735437 9 0.22752970270202458 1.1463222624122154 >10 1.1500889850513274 11.74365833844966 >50 0.08135457544218483 3.148131059470939 >100 0.03496922741276224 3.368198276491459 >500 0.0016837481108327767 0.5877615235648826 >1k 5.942640391174505E-4 0.4511475820439063 >5k 1.980880130391502E-4 0.7152272100732937 >10k+ 9.90440065195751E-5 0.6250198712524216 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11273 0.6245702720506882 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7627 0.4225669710751883 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5273 0.2921457504234257 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1966 0.1089244349198663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.540408693787706E-5 0.0 0.0 0.0 0.0 6 5.540408693787706E-5 0.0 0.0 0.0 0.0 7 5.540408693787706E-5 0.0 0.0 0.0 0.0 8 5.540408693787706E-5 0.0 0.0 0.0 0.0 9 5.540408693787706E-5 0.0 0.0 0.0 0.0 10-11 5.540408693787706E-5 0.0 0.0 0.0 0.0 12-13 5.540408693787706E-5 0.0 0.0 0.0 8.310613040681558E-5 14-15 5.540408693787706E-5 0.0 0.0 0.0 1.1080817387575411E-4 16-17 8.310613040681558E-5 0.0 0.0 0.0 1.1080817387575411E-4 18-19 1.1080817387575411E-4 0.0 0.0 5.540408693787706E-5 1.1080817387575411E-4 20-21 1.1080817387575411E-4 0.0 0.0 1.939143042825697E-4 1.1080817387575411E-4 22-23 1.1080817387575411E-4 0.0 0.0 6.371469997855862E-4 1.1080817387575411E-4 24-25 1.1080817387575411E-4 0.0 0.0 0.0010526776518196641 1.1080817387575411E-4 26-27 1.1080817387575411E-4 0.0 0.0 0.001357400129977988 1.1080817387575411E-4 28-29 2.2161634775150825E-4 0.0 0.0 0.0029918206946453613 1.1080817387575411E-4 30-31 2.770204346893853E-4 0.0 0.0 0.005955939345821784 1.1080817387575411E-4 32-33 2.770204346893853E-4 0.0 0.0 0.00903086617087396 1.662122608136312E-4 34-35 3.324245216272623E-4 0.0 0.0 0.011662560300423121 1.662122608136312E-4 36-37 3.878286085651394E-4 0.0 0.0 0.01506991164710256 1.662122608136312E-4 38-39 3.878286085651394E-4 0.0 0.0 0.018754283428471387 1.662122608136312E-4 40-41 3.878286085651394E-4 0.0 0.0 0.0218569122969925 1.662122608136312E-4 42-43 4.986367824408935E-4 0.0 0.0 0.02634464333896054 1.662122608136312E-4 44-45 5.540408693787706E-4 0.0 0.0 0.030222929424611935 1.662122608136312E-4 46-47 6.371469997855862E-4 0.0 0.0 0.03617886877043372 1.939143042825697E-4 48-49 7.202531301924018E-4 0.0 0.0 0.04235642446400702 2.2161634775150823E-4 50-51 7.202531301924018E-4 0.0 0.0 0.04839546994023561 2.770204346893853E-4 52-53 7.202531301924018E-4 0.0 0.0 0.060861389501257954 3.324245216272624E-4 54-55 7.202531301924018E-4 0.0 0.0 0.08501757140617236 3.324245216272624E-4 56-57 7.202531301924018E-4 0.0 0.0 0.12598889369673244 3.324245216272624E-4 58-59 7.202531301924018E-4 0.0 0.0 0.17330398394167945 3.324245216272624E-4 60-61 7.202531301924018E-4 0.0 0.0 0.21701780853566444 3.324245216272624E-4 62-63 7.202531301924018E-4 0.0 0.0 0.27095368716968776 3.878286085651394E-4 64-65 7.202531301924018E-4 0.0 0.0 0.336635232234541 3.878286085651394E-4 66-67 7.756572171302788E-4 0.0 0.0 0.43295523737604025 4.4323269550301645E-4 68-69 8.310613040681559E-4 0.0 0.0 0.5580022615948288 4.4323269550301645E-4 70-71 8.310613040681559E-4 0.0 0.0 0.6634362390376088 4.70934738971955E-4 72-73 8.864653910060329E-4 0.0 0.0 0.7765436825212848 5.540408693787706E-4 74-75 8.864653910060329E-4 0.0 0.0 0.8907315057002494 5.540408693787706E-4 76-77 8.864653910060329E-4 0.0 0.0 1.0217898733517976 5.540408693787706E-4 78-79 8.864653910060329E-4 0.0 0.0 1.1234009687958642 5.540408693787706E-4 80-81 0.0011634858256954182 0.0 0.0 1.2381705348876766 5.540408693787706E-4 82-83 0.0011634858256954182 0.0 0.0 1.3719437027991808 6.094449563166476E-4 84-85 0.0011911878691643566 0.0 0.0 1.5671600031247905 6.094449563166476E-4 86-87 0.0012188899126332952 0.0 0.0 1.8059516178270405 6.094449563166476E-4 88-89 0.0012188899126332952 0.0 0.0 2.0972386049029295 6.094449563166476E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11525 0.0 38.431835 1 GGTATCA 6735 0.0 38.24528 1 TATCAAC 14510 0.0 30.299723 2 ATCAACG 14630 0.0 29.986217 3 CAACGCA 14655 0.0 29.805334 5 TCAACGC 14695 0.0 29.756548 4 AACGCAG 14940 0.0 29.295511 6 ACGCAGA 16675 0.0 25.990164 7 CGCAGAG 16795 0.0 25.662281 8 GTACATG 10335 0.0 25.429087 1 TACATGG 10335 0.0 24.696085 2 GCAGAGT 17550 0.0 23.934872 9 GAGTACT 8395 0.0 23.829302 12-13 ACATGGG 10515 0.0 23.5049 3 AGTACTT 8740 0.0 22.997406 12-13 AGAGTAC 14025 0.0 22.665281 10-11 CGTAGAC 110 0.0028676044 21.604372 3 GTACTTT 9235 0.0 21.58435 14-15 CATGGGA 6800 0.0 20.619467 4 TACTTTT 10350 0.0 19.282034 14-15 >>END_MODULE