FastQCFastQC Report
Thu 26 May 2016
SRR937124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804921
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112670.6242378475290608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86030.4766413599265563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59910.3319258848448215No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20870.11562832943934943No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19080.10571099787746942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121150.037.0229381
GGTATCA72900.036.1080931
ATCAACG154000.029.0541443
TCAACGC154850.028.8946634
TATCAAC155100.028.879512
CAACGCA156850.028.5565055
AACGCAG161500.027.7667776
GTACATG104250.024.9762251
ACGCAGA183100.024.4133457
CGCAGAG183100.024.2863188
TACATGG107450.023.8271622
GTCGGGA4200.023.7497982
ACATGGG105850.022.9302043
GCAGAGT193050.022.7146519
GAGTACT97950.022.67364112-13
AGTACTT102400.022.03625312-13
AGAGTAC155950.021.85801310-11
GTACTTT103900.021.28110114-15
AATACCG1150.003724869620.6514265
CATGGGA70000.019.7457144
Produced by FastQC (version 0.11.5)