##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937124_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1804921 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.950059864115936 31.0 31.0 34.0 30.0 34.0 2 32.07015597912596 33.0 31.0 34.0 30.0 34.0 3 32.1936821611583 33.0 31.0 34.0 30.0 34.0 4 35.909925697579006 37.0 35.0 37.0 35.0 37.0 5 35.732484690465675 37.0 35.0 37.0 33.0 37.0 6 35.81793441374997 37.0 35.0 37.0 35.0 37.0 7 35.75914015073236 37.0 35.0 37.0 33.0 37.0 8 35.71036405471486 37.0 35.0 37.0 33.0 37.0 9 37.36288790478918 39.0 37.0 39.0 34.0 39.0 10-11 37.267451871854774 39.0 37.0 39.0 34.0 39.0 12-13 37.1560453338401 39.0 37.0 39.0 33.0 39.0 14-15 38.27345047234755 40.0 38.0 41.0 33.0 41.0 16-17 38.280884038691994 40.0 38.0 41.0 33.0 41.0 18-19 38.42643583846606 40.0 38.0 41.0 33.5 41.0 20-21 38.441391617694066 40.0 38.0 41.0 34.0 41.0 22-23 38.339816257886085 40.0 38.0 41.0 34.0 41.0 24-25 38.24250091832275 40.0 38.0 41.0 33.5 41.0 26-27 38.0262028088764 40.0 37.0 41.0 33.0 41.0 28-29 37.85556819384338 40.0 37.0 41.0 33.0 41.0 30-31 37.80284095536591 40.0 37.0 41.0 33.0 41.0 32-33 37.643237017021804 40.0 36.5 41.0 33.0 41.0 34-35 37.50169730420334 40.0 36.0 41.0 32.5 41.0 36-37 37.32792847997226 40.0 36.0 41.0 32.0 41.0 38-39 37.153206151404966 39.0 36.0 41.0 31.5 41.0 40-41 36.82192350800949 39.0 35.5 41.0 30.5 41.0 42-43 36.58526218045 39.0 35.0 41.0 30.0 41.0 44-45 36.30613001898698 39.0 35.0 40.5 30.0 41.0 46-47 35.87811544106363 39.0 35.0 40.0 29.5 41.0 48-49 35.464565208117136 39.0 35.0 40.0 27.5 41.0 50-51 35.164891150360596 38.0 35.0 40.0 26.5 41.0 52-53 34.90196634644951 38.0 34.0 40.0 26.0 41.0 54-55 34.494343242723644 38.0 34.0 40.0 24.0 41.0 56-57 34.28954425152126 38.0 34.0 40.0 24.5 41.0 58-59 33.960178035492966 37.5 33.0 40.0 23.5 41.0 60-61 33.574697452132256 37.0 33.0 40.0 23.0 41.0 62-63 33.193191003927595 36.5 32.0 39.0 22.0 40.0 64-65 32.86450127180082 36.0 32.0 39.0 22.0 40.0 66-67 32.52181452817048 35.5 31.5 39.0 20.5 40.0 68-69 32.06344211187083 35.0 31.0 38.0 18.5 40.0 70-71 31.61984845874141 35.0 31.0 38.0 18.0 40.0 72-73 31.202444317507528 35.0 30.5 37.0 16.0 39.0 74-75 30.71703581486392 34.0 30.0 36.5 12.0 39.0 76-77 29.28664080034528 32.5 28.5 35.0 9.0 37.5 78-79 29.968922462534373 34.0 29.5 35.5 8.0 37.5 80-81 29.834975048769447 34.0 29.0 35.0 7.0 37.0 82-83 29.463745781671328 34.0 29.0 35.0 4.0 37.0 84-85 29.0012729088974 34.0 29.0 35.0 2.0 36.0 86-87 28.609792062921315 33.5 29.0 35.0 2.0 36.0 88-89 28.205563290581694 33.0 27.5 35.0 2.0 35.0 90-91 27.71732169995252 33.0 27.0 35.0 2.0 35.0 92-93 27.18927227285848 33.0 26.0 34.5 2.0 35.0 94-95 26.602592855864607 32.0 25.0 34.0 2.0 35.0 96-97 25.79090552993732 32.0 24.0 34.0 2.0 35.0 98-99 24.86945079590741 32.0 19.0 34.0 2.0 35.0 100-101 21.708092764170843 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24749599735547534 1101 2 0.21228734460770227 1101 3 0.22261237695670388 1101 4 0.12583048524064822 1101 5 0.05306577792985223 1101 6 0.11195245592627856 1101 7 0.17781820050976194 1101 8 0.21290598833383 1101 9 0.3108154089591224 1101 10-11 0.2638477897413978 1101 12-13 0.25934078635160773 1101 14-15 0.4104932547362594 1101 16-17 0.33447777364416 1101 18-19 0.18750180962312157 1101 20-21 0.16552836538831173 1101 22-23 0.19865135639530962 1101 24-25 0.2421933795434228 1101 26-27 0.15702872292290238 1101 28-29 0.21543234854809867 1101 30-31 0.22910217307639869 1101 32-33 0.3205061185490976 1101 34-35 0.24377670338807889 1101 36-37 0.13272432482762753 1101 38-39 0.18846209865377972 1101 40-41 0.4356019638144204 1101 42-43 0.3789718738813832 1101 44-45 0.11556625929657116 1101 46-47 -0.0046124861945529005 1101 48-49 -0.08646682919953008 1101 50-51 -0.0650366618658964 1101 52-53 0.09062903602177386 1101 54-55 -4.795383963696054E-4 1101 56-57 0.1037457348030486 1101 58-59 0.15507907521762831 1101 60-61 0.21709465566699038 1101 62-63 0.11911055743210852 1101 64-65 0.13742217288098146 1101 66-67 0.1396597639126469 1101 68-69 -0.26419625015901005 1101 70-71 -0.22167628223930436 1101 72-73 -0.19144438398528152 1101 74-75 0.10514949690514541 1101 76-77 0.05323196555487897 1101 78-79 -0.1420209043680032 1101 80-81 -0.09926260650600227 1101 82-83 0.12912973596743527 1101 84-85 -0.2209577494241728 1101 86-87 -0.3933154443246707 1101 88-89 -0.1259675158378215 1101 90-91 0.03297365620231929 1101 92-93 -0.12992474049775637 1101 94-95 -0.17491115582558692 1101 96-97 -0.12182964263293528 1101 98-99 0.12973362867351668 1101 100-101 0.5402512315036923 1102 1 -0.12752672230779183 1102 2 0.009169967652610467 1102 3 0.15372037906126224 1102 4 0.17171045367227578 1102 5 -0.03600008517820186 1102 6 0.12169381049651662 1102 7 0.18158787095507023 1102 8 0.2601473429040695 1102 9 0.2932722415989062 1102 10-11 0.3175008724409807 1102 12-13 0.36952816378500586 1102 14-15 0.32069707403123715 1102 16-17 0.34594851310941266 1102 18-19 0.2296494754766556 1102 20-21 0.21888967978061658 1102 22-23 0.130038272260812 1102 24-25 0.036095840441682014 1102 26-27 0.24752263644468542 1102 28-29 0.0421545588126051 1102 30-31 0.11751813423773427 1102 32-33 0.06279249392576958 1102 34-35 0.29488787428778096 1102 36-37 0.15503682781705663 1102 38-39 0.3328144686733836 1102 40-41 0.2787340487620469 1102 42-43 0.21273568504510365 1102 44-45 0.37435009963712673 1102 46-47 0.21239497542117647 1102 48-49 0.26562457559311525 1102 50-51 0.19540541495047847 1102 52-53 0.3026713543840458 1102 54-55 0.48983889562094873 1102 56-57 0.3089894928745096 1102 58-59 0.0935386820478783 1102 60-61 0.11027277782702782 1102 62-63 0.2207081084915643 1102 64-65 0.4148977193486729 1102 66-67 0.23742845382511746 1102 68-69 0.2134861648216173 1102 70-71 0.005323048128516916 1102 72-73 0.043695760463943856 1102 74-75 0.09779400592126564 1102 76-77 0.21175004562375577 1102 78-79 -0.26113800629558526 1102 80-81 0.035688008120679626 1102 82-83 0.19263328740953511 1102 84-85 0.13258053918729829 1102 86-87 0.02594784069948375 1102 88-89 0.1994510042750619 1102 90-91 -0.08729796335094164 1102 92-93 0.020433692084633748 1102 94-95 0.06700094299299408 1102 96-97 0.44220303780901915 1102 98-99 0.09912187184566434 1102 100-101 0.09913910398659453 1103 1 0.08258640294776498 1103 2 0.14330630857305948 1103 3 0.11277380689279681 1103 4 0.07905166942803277 1103 5 -0.08155458065773047 1103 6 0.012368359585607891 1103 7 0.0709471685789822 1103 8 0.04801363702973305 1103 9 0.22026669891570094 1103 10-11 0.0999426061195905 1103 12-13 0.2435598979179332 1103 14-15 0.15862668876932418 1103 16-17 0.1397212290241825 1103 18-19 0.1942930059841288 1103 20-21 0.21206922637095715 1103 22-23 0.13617355689122235 1103 24-25 0.2024368176803435 1103 26-27 0.23109647452564275 1103 28-29 0.005762243606575623 1103 30-31 0.2649574827452952 1103 32-33 0.17249304137931887 1103 34-35 0.3505563468696664 1103 36-37 0.273416842355239 1103 38-39 0.21794349506928512 1103 40-41 0.2987598313252988 1103 42-43 0.17411862993629512 1103 44-45 0.28490646917962437 1103 46-47 0.20335744872998163 1103 48-49 0.31128242848485144 1103 50-51 0.026169010425469708 1103 52-53 -0.02497476573770996 1103 54-55 0.4646066253924275 1103 56-57 0.28238773693429664 1103 58-59 0.16976761303693166 1103 60-61 -0.006022483633152831 1103 62-63 0.38125056453626627 1103 64-65 0.21567999866356047 1103 66-67 0.2448383852230478 1103 68-69 0.019451080091258177 1103 70-71 -0.06673751250025717 1103 72-73 0.2141116888633796 1103 74-75 0.16629526665453298 1103 76-77 0.22960305093927857 1103 78-79 0.30089149031974216 1103 80-81 0.27764146321131733 1103 82-83 0.39958350548231536 1103 84-85 0.17267299296041116 1103 86-87 -0.004774866128919086 1103 88-89 0.2687538528507716 1103 90-91 0.14682900725466297 1103 92-93 0.040335388963466556 1103 94-95 0.3205375269737303 1103 96-97 0.5630457414571985 1103 98-99 0.45610247245445024 1103 100-101 1.0239349447025994 1104 1 0.10159275156871317 1104 2 0.15376715435338184 1104 3 0.07542297314237345 1104 4 0.08892364329594926 1104 5 -0.20375675306274132 1104 6 0.006133890876490966 1104 7 0.16866313584363013 1104 8 0.11706641488068215 1104 9 0.08769588061973366 1104 10-11 0.19237299520312234 1104 12-13 0.2198318722249013 1104 14-15 0.1803951924090228 1104 16-17 0.13888786276231002 1104 18-19 0.17772446634257477 1104 20-21 0.13449070279851583 1104 22-23 0.13327859012708387 1104 24-25 0.11380638117972097 1104 26-27 0.15091138324775955 1104 28-29 0.11501033155648344 1104 30-31 0.11212217150481507 1104 32-33 0.009113739815425959 1104 34-35 0.23859220384568403 1104 36-37 0.18108496446301814 1104 38-39 0.13518476461785411 1104 40-41 0.31870025263489765 1104 42-43 0.3526585019979791 1104 44-45 0.34713508319576647 1104 46-47 0.06777723702146687 1104 48-49 0.1562027633899774 1104 50-51 0.2742097627765716 1104 52-53 0.309368924716523 1104 54-55 0.4793712302939497 1104 56-57 0.22074278114633472 1104 58-59 -0.015784938704769047 1104 60-61 0.1696945900301685 1104 62-63 0.20774683702645547 1104 64-65 0.2924004595751981 1104 66-67 0.08334094925807278 1104 68-69 0.10854690650959498 1104 70-71 0.11071901086143399 1104 72-73 0.3295539080708174 1104 74-75 0.3400871188185981 1104 76-77 0.5039792199021598 1104 78-79 0.7286797686404753 1104 80-81 0.577771036984398 1104 82-83 0.756989790880489 1104 84-85 0.6172512552998306 1104 86-87 0.4670078516603873 1104 88-89 0.6456192541837176 1104 90-91 0.7273782332146403 1104 92-93 0.5421225850331162 1104 94-95 0.5909208370375687 1104 96-97 0.7762206665996665 1104 98-99 0.7011382218639319 1104 100-101 1.35285667797271 1105 1 0.03673654387502978 1105 2 0.05712549456934113 1105 3 0.015220186879659536 1105 4 0.06042628491422164 1105 5 -0.007032665611852451 1105 6 0.05276533596622812 1105 7 0.1009804075260945 1105 8 0.010713148144972706 1105 9 0.18489142810549453 1105 10-11 0.1024705755769304 1105 12-13 0.05352367577939532 1105 14-15 0.14013864517234254 1105 16-17 -0.025656796755164635 1105 18-19 0.05136105046139505 1105 20-21 0.11968158102187942 1105 22-23 0.12393269108104477 1105 24-25 0.007521121365968497 1105 26-27 0.01107836345637736 1105 28-29 0.020240910527540734 1105 30-31 0.0736150955074848 1105 32-33 0.13238215794711294 1105 34-35 0.08483183074169176 1105 36-37 0.2314875162793797 1105 38-39 0.2457205936140383 1105 40-41 0.2915471917225432 1105 42-43 0.3326230892369253 1105 44-45 0.172355791603934 1105 46-47 -0.004557137445949877 1105 48-49 -0.10317700255696849 1105 50-51 -0.05788852115478704 1105 52-53 0.13790544853041808 1105 54-55 0.3032958469424827 1105 56-57 0.05429870741699716 1105 58-59 -0.05781280679428136 1105 60-61 -0.07989600283723775 1105 62-63 -0.1219392495766769 1105 64-65 0.01465650317828704 1105 66-67 -7.715959160066177E-4 1105 68-69 0.23389486160426998 1105 70-71 -0.013637927701818597 1105 72-73 0.06956057766533164 1105 74-75 -0.093512178934823 1105 76-77 0.01874969517495728 1105 78-79 0.18346663658578066 1105 80-81 0.14222698707114034 1105 82-83 0.20135084263348446 1105 84-85 -0.14213684081224898 1105 86-87 -0.00438322036904637 1105 88-89 0.17585392995730587 1105 90-91 0.18032918738726167 1105 92-93 0.1891848225646342 1105 94-95 0.36137016341398365 1105 96-97 0.5710298539251433 1105 98-99 0.39508153631919996 1105 100-101 0.7232899721952357 1106 1 -0.0054489834595301545 1106 2 0.027818708452578278 1106 3 0.06287985421821674 1106 4 0.04480372383005715 1106 5 0.04615007824738626 1106 6 0.05500882213618041 1106 7 0.06409633616540589 1106 8 0.14135986671247025 1106 9 0.13552010766046862 1106 10-11 0.14465244673495903 1106 12-13 0.27538356153231547 1106 14-15 0.3055477277335896 1106 16-17 0.15212591217353122 1106 18-19 0.1816903847813336 1106 20-21 0.006221845228452594 1106 22-23 0.17087982586205186 1106 24-25 0.18491908802557333 1106 26-27 0.22062016860073186 1106 28-29 0.2455158057851179 1106 30-31 0.2453578008025019 1106 32-33 0.06839231874294427 1106 34-35 0.0439483241196541 1106 36-37 0.062249010665603066 1106 38-39 0.0669310299168373 1106 40-41 0.17587321917142873 1106 42-43 0.19946176494728718 1106 44-45 -0.12253467356578085 1106 46-47 0.014503635801638382 1106 48-49 0.09683357223055111 1106 50-51 0.196397431786842 1106 52-53 0.27090815100736165 1106 54-55 0.443095944084277 1106 56-57 0.415071364391423 1106 58-59 0.46538774264467264 1106 60-61 0.3452076427363764 1106 62-63 0.26441328402710695 1106 64-65 0.09885599879466866 1106 66-67 0.29273331896960997 1106 68-69 0.132701457459234 1106 70-71 0.009629136025619545 1106 72-73 0.05292342759306479 1106 74-75 -0.04444136807643062 1106 76-77 0.04662818186661255 1106 78-79 0.21357820340648814 1106 80-81 0.27299225013163664 1106 82-83 0.21786916696840208 1106 84-85 0.1339226277428054 1106 86-87 0.1430255207615616 1106 88-89 0.62669663646167 1106 90-91 0.28724394531884556 1106 92-93 0.3908590982971525 1106 94-95 0.6983167835178357 1106 96-97 0.5754864518753777 1106 98-99 0.4300410526014211 1106 100-101 0.9042677575478599 1107 1 0.2264803282209975 1107 2 0.23966938010854477 1107 3 0.21194012054186828 1107 4 0.1399683855171645 1107 5 0.10087418039172036 1107 6 0.10535714774680827 1107 7 0.1804215959844484 1107 8 0.23604040988409025 1107 9 0.1747234754414464 1107 10-11 0.1633586867770518 1107 12-13 0.18056517906114777 1107 14-15 0.2703900822568528 1107 16-17 0.23893470582385845 1107 18-19 0.3092132240077987 1107 20-21 0.30356065510376595 1107 22-23 0.16467252607984761 1107 24-25 0.17415841388469744 1107 26-27 0.2637432592413944 1107 28-29 0.37979116280791203 1107 30-31 0.38263858077122137 1107 32-33 0.21207422130532194 1107 34-35 0.2563670164734404 1107 36-37 0.30151450114255596 1107 38-39 0.42871928653472935 1107 40-41 0.35237388424970106 1107 42-43 0.29688374614968893 1107 44-45 0.34847025239858453 1107 46-47 0.39620813640825503 1107 48-49 0.470072284171259 1107 50-51 0.3267773303441359 1107 52-53 0.4573379231267012 1107 54-55 0.6609934197102589 1107 56-57 0.43931704869119415 1107 58-59 0.491135626818739 1107 60-61 0.41068993411374777 1107 62-63 0.49149541753974546 1107 64-65 0.6745907275742908 1107 66-67 0.6171934361987894 1107 68-69 0.38155167598495154 1107 70-71 0.27472962862205463 1107 72-73 0.5625618004169404 1107 74-75 0.4990293173196214 1107 76-77 0.28513723716312356 1107 78-79 0.5418951899441318 1107 80-81 0.44513277897849335 1107 82-83 0.7096970359406747 1107 84-85 0.6617286002918803 1107 86-87 0.9915794505702245 1107 88-89 0.9322300877063157 1107 90-91 0.803769897988662 1107 92-93 0.8032965005452688 1107 94-95 1.1173931286569179 1107 96-97 1.0291345889253556 1107 98-99 1.344268757862114 1107 100-101 1.5293990922357104 1108 1 0.03891437340491777 1108 2 0.08134744803226823 1108 3 0.04316917157201772 1108 4 0.0392587748622546 1108 5 -0.021570750864746913 1108 6 0.018903991457747793 1108 7 0.01619995017155418 1108 8 0.0039947690169697125 1108 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-0.8672087732049647 2316 86-87 -0.8917858310109175 2316 88-89 -1.594017679885063 2316 90-91 -1.6069792983442355 2316 92-93 -1.5583718528301382 2316 94-95 -1.8639689382558124 2316 96-97 -1.325515071748537 2316 98-99 -1.726127122599845 2316 100-101 -2.2941176954771088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 16.0 5 53.0 6 114.0 7 306.0 8 710.0 9 1330.0 10 2313.0 11 3683.0 12 5339.0 13 7441.0 14 9517.0 15 12855.0 16 16682.0 17 22347.0 18 19455.0 19 11861.0 20 10781.0 21 11218.0 22 12122.0 23 13344.0 24 15743.0 25 18810.0 26 22809.0 27 27678.0 28 34688.0 29 42694.0 30 52026.0 31 64297.0 32 79920.0 33 102212.0 34 134849.0 35 184711.0 36 269723.0 37 367748.0 38 215459.0 39 10061.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88380588429576 19.918848765220012 15.318597841434785 21.878747509049436 2 15.398586156209848 21.573168549261524 39.866538642459524 23.1617066520691 3 18.865399979389647 26.39416305893121 30.530749873401454 24.209687088277697 4 12.775018962048753 17.86238843694544 37.64563656802707 31.71695603297873 5 13.165307388856876 38.37058419396303 33.79382562829662 14.67028278888347 6 30.36005453978318 37.250494619986135 17.822331282089355 14.567119558141325 7 27.20102431075931 33.96204044387539 21.223976007814194 17.612959237551117 8 26.317454513219424 34.64563526361279 20.022494071759414 19.014416151408373 9 26.96796919972586 16.42849346701985 20.576546648353748 36.02699068490054 10-11 24.43638807460271 27.391974496390702 27.336237984931195 20.835399444075392 12-13 25.962654075073054 24.98268508124437 28.09759740957734 20.957063434105237 14-15 22.811289126443768 25.641493747885928 27.19531799944539 24.351899126224914 16-17 21.26901953049469 29.147785415538962 27.477213684144626 22.105981369821727 18-19 21.191409225818642 27.176046712500824 30.826859798782547 20.805684262897987 20-21 22.427856826456637 26.075287806723974 30.811366055912774 20.68548931090661 22-23 21.96483832961704 26.293990525192235 30.726057494314755 21.015113650875968 24-25 21.664643941784306 26.389260175792845 30.920958043025447 21.0251378393974 26-27 21.610944329003587 26.59585637341118 30.726989309477755 21.066209988107484 28-29 21.3039695122137 26.726759836103493 30.867553926920287 21.101716724762518 30-31 21.573603498435663 26.36915964743055 30.82722180084336 21.230015053290423 32-33 21.396862403330015 26.587157910075064 30.683303078009427 21.332676608585494 34-35 21.766797549588045 26.544125753980367 30.570285347668957 21.118791348762635 36-37 21.86795575901545 26.27925563227625 30.548136656716828 21.304651951991467 38-39 21.6775219166708 26.60085270207485 30.538857953265676 21.182767427988676 40-41 21.788522302232412 26.490421400746524 30.29047447821469 21.430581818806377 42-43 21.62172194794121 26.66133309989745 30.104253870391002 21.61269108177034 44-45 21.82894843277157 26.65828580546577 29.888786452036836 21.623979309725826 46-47 22.12305489778483 26.964188954844488 29.29440561842699 21.618350528943697 48-49 22.145952256029215 27.186095331701864 28.876974534591305 21.790977877677616 50-51 22.01960235693687 27.422827493164387 28.754574391166198 21.802995758732553 52-53 22.2814218663041 27.546145665475198 28.336600810179124 21.83583165804158 54-55 22.098778838519802 27.36144684448793 28.390328440967778 22.14944587602449 56-57 22.213926971630244 27.714324497275495 28.09475792488843 21.97699060620583 58-59 22.485398228617587 27.351214847433514 28.174465543587026 21.988921380361877 60-61 22.290832470395554 27.27980404248258 28.676765947450278 21.75259753967159 62-63 22.19067266397606 27.34571965912072 28.716555659930727 21.747052016972493 64-65 22.089550833029072 27.415496207863065 28.60194834228018 21.893004616827678 66-67 22.231948720179087 27.58007901179027 28.166891561151864 22.021080706878777 68-69 22.102681616183332 27.482867652232596 28.441220907974262 21.973229823609813 70-71 22.1526312640173 27.40637770705718 28.359582073807477 22.081408955118036 72-73 22.18775460001014 27.360298784236043 28.24577009049459 22.20617652525923 74-75 22.023475935517776 27.820293932608426 27.992993399245762 22.16323673262804 76-77 22.04610787350904 27.656219571011338 28.1025552663152 22.195117289164422 78-79 22.019611993023496 27.658544774382854 27.999114640238105 22.322728592355546 80-81 21.940382400777 27.794434719302295 28.047082184946255 22.21810069497445 82-83 21.994742149236533 27.714109212596682 28.07329410721805 22.217854530948742 84-85 22.01722221668168 27.672479801958914 27.84091223894357 22.46938574241583 86-87 22.076191953637707 27.601604507691498 27.852253430216933 22.469950108453858 88-89 22.068970866728943 27.48041695893019 27.755698723126994 22.69491345121388 90-91 21.94937831684164 27.540440639736808 27.880348950370337 22.629832093051217 92-93 21.988912534121994 27.48882084035811 27.839030073892427 22.683236551627466 94-95 21.84838006760407 27.48197843562128 27.793321702168683 22.87631979460597 96-97 21.897162555719337 27.582494813471982 27.664022130663664 22.85632050014502 98-99 21.72521306493546 27.64864681431043 27.49800197517047 23.128138145583637 100-101 21.583870418157414 27.714546514800826 27.618944723548488 23.082638343493276 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1367.0 1 966.0 2 608.5 3 648.0 4 560.5 5 542.5 6 954.0 7 1932.5 8 3137.5 9 3687.5 10 3214.5 11 2643.0 12 2628.5 13 2769.5 14 2929.0 15 3161.5 16 3342.5 17 3575.5 18 3881.0 19 4122.5 20 4490.0 21 5123.5 22 6005.5 23 7114.5 24 8291.0 25 10060.5 26 12358.5 27 14763.5 28 17114.0 29 19757.0 30 22858.0 31 25971.5 32 29060.5 33 32346.5 34 36265.0 35 39386.0 36 42959.0 37 47385.5 38 50137.0 39 51633.5 40 53251.5 41 55774.0 42 58037.0 43 60444.0 44 63418.5 45 65417.5 46 65691.0 47 67231.0 48 71588.5 49 71453.5 50 67341.5 51 64086.5 52 61246.5 53 59327.0 54 56598.5 55 51832.0 56 46800.0 57 42158.0 58 38123.5 59 34175.5 60 29163.5 61 23820.5 62 18814.0 63 14110.0 64 10268.0 65 7617.0 66 5483.0 67 4043.0 68 2881.5 69 1830.0 70 1213.0 71 789.0 72 540.5 73 441.5 74 324.0 75 218.0 76 152.0 77 76.5 78 30.0 79 13.0 80 8.5 81 7.0 82 4.5 83 3.0 84 2.5 85 1.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021552189818834173 2 0.0029918206946453613 3 1.6621226081363116E-4 4 0.0 5 0.0022715675644529596 6 0.0 7 0.0 8 5.5404086937877064E-5 9 1.1080817387575413E-4 10-11 0.0 12-13 0.006593086345607371 14-15 0.0028533104773006686 16-17 0.0 18-19 4.432326955030165E-4 20-21 0.0011357837822264798 22-23 0.0016344205646673733 24-25 6.925510867234632E-4 26-27 2.4931839122044677E-4 28-29 8.310613040681558E-5 30-31 0.0 32-33 2.4931839122044677E-4 34-35 0.0 36-37 0.0018560369124188815 38-39 0.010388266300851948 40-41 6.648490432545247E-4 42-43 0.0 44-45 8.31061304068156E-4 46-47 1.6621226081363116E-4 48-49 1.1080817387575413E-4 50-51 0.001994547129763574 52-53 6.371469997855861E-4 54-55 0.0 56-57 3.324245216272623E-4 58-59 1.6621226081363116E-4 60-61 0.0022161634775150823 62-63 2.7702043468938532E-5 64-65 1.1080817387575413E-4 66-67 2.7702043468938532E-5 68-69 1.1080817387575413E-4 70-71 4.986367824408935E-4 72-73 3.601265650962009E-4 74-75 0.0028533104773006686 76-77 8.310613040681558E-5 78-79 2.770204346893853E-4 80-81 0.0019114409993567587 82-83 5.5404086937877064E-5 84-85 0.0023823757383287136 86-87 3.324245216272623E-4 88-89 0.0023823757383287136 90-91 0.0012465919561022339 92-93 0.0 94-95 0.0 96-97 2.4931839122044677E-4 98-99 7.479551736613403E-4 100-101 0.002133057347108267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1804921.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.165864787306425 #Duplication Level Percentage of deduplicated Percentage of total 1 74.3567593039655 40.27598170480884 2 14.292339987103686 15.483139104713425 3 4.893267604404776 7.951442142848879 4 2.1799745357786344 4.723208237790264 5 1.2013575707560686 3.253628586939006 6 0.694954648630014 2.2585691718602035 7 0.4561750145090102 1.7296379908669817 8 0.31591561019721165 1.3689473780913257 9 0.23165242435236316 1.1292888505609653 >10 1.244792176776406 12.280535130966763 >50 0.0918205056262008 3.4067619900197514 >100 0.038224232486085113 3.536186361423569 >500 0.0018442569426766506 0.6407758809481118 >1k 6.147523142255501E-4 0.5266727100465599 >5k 2.0491743807518338E-4 0.8099327218566382 >10k+ 1.0245871903759169E-4 0.6252920362586502 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11267 0.6242378475290608 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8603 0.4766413599265563 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5991 0.3319258848448215 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2087 0.11562832943934943 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1908 0.10571099787746942 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.1080817387575411E-4 0.0 0.0 0.0 0.0 8 1.1080817387575411E-4 0.0 0.0 0.0 0.0 9 1.1080817387575411E-4 0.0 0.0 0.0 0.0 10-11 1.1080817387575411E-4 0.0 0.0 0.0 0.0 12-13 1.662122608136312E-4 0.0 0.0 0.0 0.0 14-15 1.662122608136312E-4 0.0 0.0 0.0 5.540408693787706E-5 16-17 1.662122608136312E-4 0.0 0.0 2.770204346893853E-5 1.1080817387575411E-4 18-19 1.939143042825697E-4 0.0 0.0 1.1080817387575411E-4 1.1080817387575411E-4 20-21 2.2161634775150823E-4 0.0 0.0 2.770204346893853E-4 1.1080817387575411E-4 22-23 2.2161634775150823E-4 0.0 0.0 7.479551736613403E-4 1.1080817387575411E-4 24-25 2.770204346893853E-4 0.0 0.0 0.0011634858256954182 1.1080817387575411E-4 26-27 2.770204346893853E-4 0.0 0.0 0.0015236123907916193 1.1080817387575411E-4 28-29 2.770204346893853E-4 0.0 0.0 0.0030472247815832385 1.1080817387575411E-4 30-31 2.770204346893853E-4 0.0 0.0 0.006094449563166477 1.1080817387575411E-4 32-33 3.047224781583238E-4 0.0 0.0 0.009280184562094408 1.1080817387575411E-4 34-35 4.1553065203407794E-4 0.0 0.0 0.011939580735112507 1.1080817387575411E-4 36-37 4.70934738971955E-4 0.0 0.0 0.015430038212198762 1.1080817387575411E-4 38-39 4.986367824408935E-4 0.0 0.0 0.019142112037036522 1.1080817387575411E-4 40-41 5.263388259098321E-4 0.0 0.0 0.022189336818619763 1.1080817387575411E-4 42-43 5.540408693787706E-4 0.0 0.0 0.026704769904056742 1.1080817387575411E-4 44-45 6.094449563166476E-4 0.0 0.0 0.030444545772363444 1.1080817387575411E-4 46-47 7.756572171302788E-4 0.0 0.0 0.036372783074716294 1.1080817387575411E-4 48-49 8.310613040681559E-4 0.0 0.0 0.04243953059441383 1.662122608136312E-4 50-51 8.310613040681559E-4 0.0 0.0 0.04842317198370455 2.2161634775150823E-4 52-53 8.310613040681559E-4 0.0 0.0 0.06083368745778901 3.324245216272623E-4 54-55 8.310613040681559E-4 0.0 0.0 0.08493446527576554 3.878286085651394E-4 56-57 8.864653910060329E-4 0.0 0.0 0.1260997018706082 3.878286085651394E-4 58-59 8.864653910060329E-4 0.0 0.0 0.17335938802861733 3.878286085651394E-4 60-61 8.864653910060329E-4 0.0 0.0 0.21759955144851215 3.878286085651394E-4 62-63 8.864653910060329E-4 0.0 0.0 0.2717016423433491 3.878286085651394E-4 64-65 8.864653910060329E-4 0.0 0.0 0.33749399558207815 3.878286085651394E-4 66-67 9.4186947794391E-4 0.0 0.0 0.43414642524520464 3.878286085651394E-4 68-69 9.4186947794391E-4 0.0 0.0 0.5600799148549992 3.878286085651394E-4 70-71 9.695715214128486E-4 0.0 0.0 0.6670652067320397 3.878286085651394E-4 72-73 9.97273564881787E-4 0.0 0.0 0.7805604788242809 3.878286085651394E-4 74-75 9.97273564881787E-4 0.0 0.0 0.8953023428726243 3.878286085651394E-4 76-77 9.97273564881787E-4 0.0 0.0 1.027551898393337 3.878286085651394E-4 78-79 9.97273564881787E-4 0.0 0.0 1.129301504054748 3.878286085651394E-4 80-81 0.0012188899126332952 0.0 0.0 1.2445143028420635 3.878286085651394E-4 82-83 0.0012188899126332952 0.0 0.0 1.3791739361445736 4.986367824408935E-4 84-85 0.0012188899126332952 0.0 0.0 1.574140918078963 4.986367824408935E-4 86-87 0.0012742939995711725 0.0 0.0 1.8125447041726481 4.986367824408935E-4 88-89 0.0013296980865090495 0.0 0.0 2.1031668422052823 4.986367824408935E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12115 0.0 37.022938 1 GGTATCA 7290 0.0 36.108093 1 ATCAACG 15400 0.0 29.054144 3 TCAACGC 15485 0.0 28.894663 4 TATCAAC 15510 0.0 28.87951 2 CAACGCA 15685 0.0 28.556505 5 AACGCAG 16150 0.0 27.766777 6 GTACATG 10425 0.0 24.976225 1 ACGCAGA 18310 0.0 24.413345 7 CGCAGAG 18310 0.0 24.286318 8 TACATGG 10745 0.0 23.827162 2 GTCGGGA 420 0.0 23.749798 2 ACATGGG 10585 0.0 22.930204 3 GCAGAGT 19305 0.0 22.714651 9 GAGTACT 9795 0.0 22.673641 12-13 AGTACTT 10240 0.0 22.036253 12-13 AGAGTAC 15595 0.0 21.858013 10-11 GTACTTT 10390 0.0 21.281101 14-15 AATACCG 115 0.0037248696 20.651426 5 CATGGGA 7000 0.0 19.745714 4 >>END_MODULE