FastQCFastQC Report
Thu 26 May 2016
SRR937123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753847
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61450.815152146257795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42440.5629789599215755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28600.3793873292591202No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20430.2710098998868471No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14890.19752018645693356No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12470.16541818167346956No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9940.13185699485439353No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.12880597787084117No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8280.10983661140788516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79600.027.7609021
GTACATG48000.024.651981
TATCAAC93050.023.8008292
CAACGCA92950.023.768995
TCAACGC93550.023.6704754
TACATGG49850.023.6433532
GAGTACT39850.023.60717612-13
ATCAACG94100.023.5826233
AACGCAG94200.023.5544596
ACATGGG48850.023.0541253
GGTATCA56600.022.501531
GTACTTT42800.021.98077614-15
AGTACTT40850.021.69171512-13
CTACGAG1100.0028698821.5994243
ACGCAGA103250.021.213787
CGCAGAG103200.021.0859388
AGAGTAC70500.020.8232110-11
TATAGGT1604.9712307E-520.7894483
GTACTAT1150.003712179120.6616921
CATGGGA32900.020.654044
Produced by FastQC (version 0.11.5)