##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937123_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 753847 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.69485319965457 33.0 31.0 34.0 30.0 34.0 2 31.75891659713443 33.0 31.0 34.0 30.0 34.0 3 31.750792932783444 33.0 31.0 34.0 30.0 34.0 4 35.34587389748848 37.0 35.0 37.0 33.0 37.0 5 35.37555631315108 37.0 35.0 37.0 33.0 37.0 6 35.44809623172872 37.0 35.0 37.0 33.0 37.0 7 35.372514581871386 37.0 35.0 37.0 33.0 37.0 8 35.34022420995242 37.0 35.0 37.0 33.0 37.0 9 36.932232933207935 39.0 37.0 39.0 33.0 39.0 10-11 36.80895194913557 39.0 37.0 39.0 33.0 39.0 12-13 36.70469737227846 39.0 37.0 39.0 32.0 39.0 14-15 37.794769363013984 40.0 37.5 41.0 32.5 41.0 16-17 37.737138968517485 40.0 37.0 41.0 32.0 41.0 18-19 37.74480365379182 40.0 37.5 41.0 32.0 41.0 20-21 37.72753622419403 40.0 37.0 41.0 32.0 41.0 22-23 37.59751580891083 40.0 37.0 41.0 32.0 41.0 24-25 37.42475064568805 40.0 37.0 41.0 32.0 41.0 26-27 37.34264048275048 40.0 37.0 41.0 32.0 41.0 28-29 37.385013802535525 40.0 37.0 41.0 32.0 41.0 30-31 37.198554879173095 39.5 36.5 41.0 31.0 41.0 32-33 36.95412928618141 39.0 36.0 41.0 31.0 41.0 34-35 36.7228542396534 39.0 35.0 41.0 30.0 41.0 36-37 36.54213587107198 39.0 35.0 41.0 30.0 41.0 38-39 36.35273802243691 39.0 35.0 41.0 30.0 41.0 40-41 36.11742303146394 39.0 35.0 40.5 30.0 41.0 42-43 35.8759363637449 39.0 35.0 40.0 29.0 41.0 44-45 35.548011068559006 38.5 35.0 40.0 27.5 41.0 46-47 35.09298504869025 38.0 35.0 40.0 25.5 41.0 48-49 34.648283404988014 38.0 34.0 40.0 24.0 41.0 50-51 33.949451944492715 37.5 33.5 39.5 23.0 40.5 52-53 34.255411243926154 38.0 34.0 40.0 23.0 40.5 54-55 34.558225342808285 38.0 34.0 40.0 23.0 41.0 56-57 34.43814858983322 38.0 34.0 40.0 22.5 41.0 58-59 34.19490626081951 38.0 34.0 40.0 22.0 41.0 60-61 33.88724237146265 38.0 34.0 40.0 21.0 41.0 62-63 33.59617402470262 37.5 33.5 40.0 20.0 41.0 64-65 33.279033411289035 37.0 33.0 40.0 18.5 41.0 66-67 32.89087639799588 36.5 33.0 39.5 17.0 41.0 68-69 32.453710102978455 36.0 32.0 39.0 13.0 41.0 70-71 32.07283440804301 35.0 32.0 39.0 10.0 40.5 72-73 31.69433187370912 35.0 31.5 38.0 7.5 40.0 74-75 31.27294198955491 35.0 31.0 37.0 7.0 39.5 76-77 30.845890479102522 35.0 31.0 37.0 2.0 39.0 78-79 30.415579686594228 35.0 30.5 36.0 2.0 39.0 80-81 29.98272925407941 34.5 30.0 36.0 2.0 37.5 82-83 29.57607777174944 34.0 30.0 35.0 2.0 37.0 84-85 29.22527183898059 34.0 29.0 35.0 2.0 37.0 86-87 28.920889782674735 34.0 29.0 35.0 2.0 36.0 88-89 28.528467978250227 34.0 29.0 35.0 2.0 36.0 90-91 28.16170588992196 34.0 29.0 35.0 2.0 35.5 92-93 27.801267365924385 34.0 27.0 35.0 2.0 35.0 94-95 27.401897865216682 34.0 27.0 35.0 2.0 35.0 96-97 26.949630362659796 34.0 25.5 35.0 2.0 35.0 98-99 26.356852252512777 33.0 25.0 35.0 2.0 35.0 100-101 24.412474281916623 31.0 13.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5872675711529354 1101 2 0.39356541606871787 1101 3 0.6514133647373193 1101 4 0.49332863920825787 1101 5 0.5388173666270717 1101 6 0.44052037229681673 1101 7 0.567184708602305 1101 8 0.42063988837114863 1101 9 0.7247778503444309 1101 10-11 0.5674884977223158 1101 12-13 0.812448581546036 1101 14-15 1.030013666659201 1101 16-17 1.0881257117572645 1101 18-19 1.077671526113427 1101 20-21 0.9254918377650725 1101 22-23 0.9158152150425849 1101 24-25 1.0866001471508753 1101 26-27 1.0992124364741116 1101 28-29 1.0958704284802039 1101 30-31 0.998384775581556 1101 32-33 1.002045488421203 1101 34-35 1.0229791043794734 1101 36-37 1.151768653325668 1101 38-39 1.2655842294792805 1101 40-41 1.2541630818743315 1101 42-43 1.3871675737795002 1101 44-45 1.4117792245845209 1101 46-47 1.5557885723017506 1101 48-49 1.5431626013093123 1101 50-51 1.5907911829371102 1101 52-53 1.751957814896464 1101 54-55 1.6869712932777787 1101 56-57 1.5617876257786563 1101 58-59 1.646196107584025 1101 60-61 1.7422317290611744 1101 62-63 1.8722871848244793 1101 64-65 1.747918107403727 1101 66-67 1.699538699375033 1101 68-69 1.7000028508711473 1101 70-71 1.6392720074050438 1101 72-73 1.7680177442199785 1101 74-75 1.577699055671193 1101 76-77 1.695111996299545 1101 78-79 1.7702178117214729 1101 80-81 1.8293795810162408 1101 82-83 1.7858824961582158 1101 84-85 1.769672892842678 1101 86-87 1.9719806451801496 1101 88-89 1.9627091791823652 1101 90-91 1.933635771185724 1101 92-93 1.9216303093618698 1101 94-95 2.0778096873765293 1101 96-97 2.0698460602465154 1101 98-99 2.1246248490608046 1101 100-101 1.907617937332649 1102 1 0.5731238128765952 1102 2 0.43146443869713735 1102 3 0.535902467163627 1102 4 0.46202382293978417 1102 5 0.37114194793361577 1102 6 0.32619934434499953 1102 7 0.37652128745678937 1102 8 0.4115352461878672 1102 9 0.655019711994477 1102 10-11 0.5944381987787679 1102 12-13 0.5708032111240655 1102 14-15 0.8267090003269075 1102 16-17 0.7028535908635902 1102 18-19 0.5555451591068206 1102 20-21 0.5484793094695917 1102 22-23 0.6697911774868857 1102 24-25 0.7833525693499155 1102 26-27 0.8480389868540001 1102 28-29 0.9055164994889111 1102 30-31 0.8790359099217113 1102 32-33 0.8489210108758058 1102 34-35 0.8468702732712998 1102 36-37 1.0153166143263448 1102 38-39 1.059529627500126 1102 40-41 1.2679837475909608 1102 42-43 1.2807079119187534 1102 44-45 1.2954624200561824 1102 46-47 1.2973140269533516 1102 48-49 1.2987470604908466 1102 50-51 1.1178851809786536 1102 52-53 1.4677755177149905 1102 54-55 1.6204263336543931 1102 56-57 1.602904139457344 1102 58-59 1.4513192144739264 1102 60-61 1.5972232105632855 1102 62-63 1.7271262422699394 1102 64-65 1.8242095820061266 1102 66-67 1.5424953147449472 1102 68-69 1.4867398681230988 1102 70-71 1.6398916551479097 1102 72-73 1.7386813378584414 1102 74-75 1.5965601643497074 1102 76-77 1.494348947208053 1102 78-79 1.6408761310563413 1102 80-81 1.678491209163905 1102 82-83 1.6836066175720212 1102 84-85 1.9011845016180544 1102 86-87 1.9827292778418197 1102 88-89 1.8928213394044278 1102 90-91 1.817982732788792 1102 92-93 1.825709966605114 1102 94-95 1.8013071411152488 1102 96-97 1.7084970526117118 1102 98-99 1.8797682902154982 1102 100-101 2.0757297300120534 1103 1 0.44798752910078576 1103 2 0.610701909270265 1103 3 0.6985219975835584 1103 4 0.5854014383730117 1103 5 0.4218143390975655 1103 6 0.34926342028891355 1103 7 0.43609292237599107 1103 8 0.31455762103248475 1103 9 0.44728677958428165 1103 10-11 0.5302537486707806 1103 12-13 0.3949710988470727 1103 14-15 0.44202634187314516 1103 16-17 0.8062265709123437 1103 18-19 0.8331973108279911 1103 20-21 0.6150153057473986 1103 22-23 0.5796095120817881 1103 24-25 0.7358416145648761 1103 26-27 0.8359874212398708 1103 28-29 0.9463894795376575 1103 30-31 0.8176587319854463 1103 32-33 0.9637348836961337 1103 34-35 0.888150802573179 1103 36-37 0.9241136886522199 1103 38-39 0.9628171914753736 1103 40-41 1.0533463492395612 1103 42-43 1.1624075181714844 1103 44-45 1.2167420465865533 1103 46-47 1.2446394795543725 1103 48-49 1.1355928115620202 1103 50-51 1.2091588526892707 1103 52-53 1.2891981988074903 1103 54-55 1.3220694646048798 1103 56-57 1.3775450273678445 1103 58-59 1.4310927924351873 1103 60-61 1.4097548943710478 1103 62-63 1.5564690072507403 1103 64-65 1.4946315756033997 1103 66-67 1.419951460882821 1103 68-69 1.5217066391871832 1103 70-71 1.582855592898344 1103 72-73 1.565578373295697 1103 74-75 1.5612973883967705 1103 76-77 1.474476255351746 1103 78-79 1.674572511637031 1103 80-81 1.5970798821592318 1103 82-83 1.73919607528412 1103 84-85 1.8342379179137787 1103 86-87 1.8597257917549186 1103 88-89 1.9883614745423763 1103 90-91 1.8445003437776073 1103 92-93 1.6753889618898654 1103 94-95 1.8503076688893607 1103 96-97 1.8636222774365372 1103 98-99 1.891304234410331 1103 100-101 2.197562651311209 1104 1 0.6141923934188611 1104 2 0.55521681817957 1104 3 0.5077281089807819 1104 4 0.5870173919061941 1104 5 0.5028688090747409 1104 6 0.6319949918214576 1104 7 0.5502042117763892 1104 8 0.49591683799730646 1104 9 0.6012691332014484 1104 10-11 0.7647769856285933 1104 12-13 0.8342955196078847 1104 14-15 0.9322969254182283 1104 16-17 0.7487743862538281 1104 18-19 0.817477941943686 1104 20-21 0.8175794792488844 1104 22-23 0.7654849573911946 1104 24-25 0.7506636255828738 1104 26-27 0.8249676538293542 1104 28-29 0.9356137367168742 1104 30-31 1.0044474960718404 1104 32-33 1.0305366151357163 1104 34-35 0.8408990585776763 1104 36-37 1.0142201230982764 1104 38-39 1.1157915249122752 1104 40-41 1.2957400885900867 1104 42-43 1.2104874560870869 1104 44-45 0.9289288757849476 1104 46-47 1.1821631549051972 1104 48-49 1.052161939330169 1104 50-51 1.2138233401595926 1104 52-53 1.3185208745087422 1104 54-55 1.0932134149941106 1104 56-57 1.1981252383923646 1104 58-59 1.3039520796280115 1104 60-61 1.1497197378051496 1104 62-63 0.9808916124402387 1104 64-65 1.224049449269593 1104 66-67 1.1099600726808418 1104 68-69 1.1941149774506705 1104 70-71 1.1200583475387056 1104 72-73 1.0869989794698007 1104 74-75 1.231631162960607 1104 76-77 1.1914247562320774 1104 78-79 1.3780301414488925 1104 80-81 1.4269702697185167 1104 82-83 1.2369423946981222 1104 84-85 1.2574701938545125 1104 86-87 1.3405712802084437 1104 88-89 1.3183692634667814 1104 90-91 1.0746999473795213 1104 92-93 0.9837140310183443 1104 94-95 1.2144303082706713 1104 96-97 1.3734627108923085 1104 98-99 1.6572513122868067 1104 100-101 1.70203844534878 1105 1 0.032060731976852 1105 2 0.17531996308805375 1105 3 0.06660834628148393 1105 4 0.028454004977952252 1105 5 -0.09228405437623621 1105 6 0.14664426542512388 1105 7 0.2830892355401673 1105 8 0.03924107030930202 1105 9 9.994733099887299E-4 1105 10-11 0.03378126647935886 1105 12-13 0.05921546432760749 1105 14-15 0.21597576048749545 1105 16-17 0.3376188936189166 1105 18-19 0.24041083033819177 1105 20-21 0.37701420470140334 1105 22-23 0.4648579981435432 1105 24-25 0.4250054869102726 1105 26-27 0.5111054500338881 1105 28-29 0.5208852505200952 1105 30-31 0.4460256805866223 1105 32-33 0.6051734169694072 1105 34-35 0.4776992068906125 1105 36-37 0.5015764449373492 1105 38-39 0.6107168628298254 1105 40-41 0.882590798132675 1105 42-43 0.7344517935664427 1105 44-45 0.5439287072475167 1105 46-47 0.7370372574433688 1105 48-49 0.8598742122259821 1105 50-51 0.6585632869017672 1105 52-53 0.5227258834412254 1105 54-55 0.689712892528064 1105 56-57 0.7335282113926667 1105 58-59 0.8093638165747947 1105 60-61 0.7720307230749768 1105 62-63 0.946139193928083 1105 64-65 1.0643686591661137 1105 66-67 0.9477495357205825 1105 68-69 1.1068850586857089 1105 70-71 1.0318896752765916 1105 72-73 1.2375484114986541 1105 74-75 1.2206829713672498 1105 76-77 1.2594970250829896 1105 78-79 1.10403883798039 1105 80-81 1.1562485924339931 1105 82-83 1.2468641933295963 1105 84-85 1.2930821532706993 1105 86-87 1.1829011416706727 1105 88-89 1.1661411986135768 1105 90-91 0.9270729544243892 1105 92-93 1.2339938031557374 1105 94-95 1.2275610596105473 1105 96-97 1.0068533469525782 1105 98-99 1.0686377012037873 1105 100-101 1.0831673090694132 1106 1 0.13073537424755344 1106 2 0.27333779090884036 1106 3 0.3646789538348507 1106 4 0.10483214243983241 1106 5 -0.02027490588925218 1106 6 -0.17901254902479025 1106 7 0.17978757004637913 1106 8 0.09736914912703298 1106 9 -0.09581028507612643 1106 10-11 0.22435715200598594 1106 12-13 0.16024760132033578 1106 14-15 0.5587420049162972 1106 16-17 0.3573673102269339 1106 18-19 0.38257010784229806 1106 20-21 0.4840690956232905 1106 22-23 0.5758977588754206 1106 24-25 0.6433699016416838 1106 26-27 0.6030799984739517 1106 28-29 0.3610506270155156 1106 30-31 0.3631439071876059 1106 32-33 0.32140904915331703 1106 34-35 0.21758602368630164 1106 36-37 0.34500243602344227 1106 38-39 0.16418859635082583 1106 40-41 -0.2940219159184636 1106 42-43 -0.10826167112978169 1106 44-45 -0.4138005913684779 1106 46-47 -0.3762896250337562 1106 48-49 0.09322513880351124 1106 50-51 0.14586096459353826 1106 52-53 0.2898998219821536 1106 54-55 0.3934221348456859 1106 56-57 0.3160669165298984 1106 58-59 0.35351992729493986 1106 60-61 -0.002840259800933609 1106 62-63 0.501074098924569 1106 64-65 0.4744085371862852 1106 66-67 0.7563233100579012 1106 68-69 0.7327119411628367 1106 70-71 0.7120708856917979 1106 72-73 0.8727319676797443 1106 74-75 0.5450026992584078 1106 76-77 0.7218474825073393 1106 78-79 0.39747949010330785 1106 80-81 0.578354503764043 1106 82-83 0.7215240572751753 1106 84-85 0.6694749517694092 1106 86-87 0.9232013997049222 1106 88-89 1.1333679168870923 1106 90-91 0.9913117533922531 1106 92-93 0.8470274648962928 1106 94-95 0.7174713340651522 1106 96-97 0.6944237199293575 1106 98-99 0.8350521980370011 1106 100-101 0.8792645410656554 1107 1 0.15719595848124612 1107 2 0.2595322686999566 1107 3 0.17945486419899837 1107 4 0.30891377509289697 1107 5 0.20651581039726352 1107 6 0.4498389915914558 1107 7 0.45144623351176705 1107 8 0.30920388302059365 1107 9 0.5521488985973448 1107 10-11 0.41365787229410245 1107 12-13 0.46792067054802544 1107 14-15 0.4086819809066995 1107 16-17 0.5262598672497418 1107 18-19 0.5972059621840344 1107 20-21 0.5715290796168873 1107 22-23 0.3842460982111575 1107 24-25 0.43986349908065847 1107 26-27 0.5206220152806722 1107 28-29 0.4997561092083984 1107 30-31 0.4301957279159012 1107 32-33 0.578761990329788 1107 34-35 0.7803361237263218 1107 36-37 0.5632397309414117 1107 38-39 0.46735986485823133 1107 40-41 0.568222982040723 1107 42-43 0.6914582168254029 1107 44-45 0.5172468275991022 1107 46-47 0.6936633561587158 1107 48-49 0.7231771195958245 1107 50-51 0.6648935776387503 1107 52-53 0.46699566031749384 1107 54-55 0.6801118107160349 1107 56-57 0.7897063723122031 1107 58-59 0.9561859937215118 1107 60-61 0.6596997562054696 1107 62-63 0.8533400052871087 1107 64-65 1.0184761642371072 1107 66-67 1.0139513612783944 1107 68-69 1.1615334697742803 1107 70-71 0.8125860917742322 1107 72-73 0.8199858692403659 1107 74-75 1.0508300301907845 1107 76-77 0.9603929006746057 1107 78-79 0.7632257886227158 1107 80-81 0.9010085653886897 1107 82-83 0.8059328207805798 1107 84-85 0.7918989213572445 1107 86-87 1.001832852285954 1107 88-89 1.2750745995601633 1107 90-91 0.9415168354250625 1107 92-93 1.1810610783416742 1107 94-95 1.1544711339851226 1107 96-97 1.1737904666280379 1107 98-99 1.6525541988373185 1107 100-101 1.8150638607935505 1108 1 -0.13378029117354373 1108 2 0.12082749101423218 1108 3 0.273213494411646 1108 4 -0.14413786809959106 1108 5 0.10091652843095744 1108 6 0.0978989197880864 1108 7 -0.1561207369383837 1108 8 -0.05688973752621962 1108 9 -0.3480858431866949 1108 10-11 0.023209791653393097 1108 12-13 3.3574925556223434E-4 1108 14-15 0.04940622835215436 1108 16-17 0.1595793922428399 1108 18-19 0.3249745344016546 1108 20-21 0.23175760764781472 1108 22-23 0.15078158564556787 1108 24-25 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-0.7075016698194787 2316 62-63 -0.27940286458811414 2316 64-65 -0.47383666998749874 2316 66-67 -0.4923432020014644 2316 68-69 -0.1883996853059351 2316 70-71 -0.5562307531455559 2316 72-73 -0.35013445161524714 2316 74-75 -0.46359733166304906 2316 76-77 -0.7975170815000823 2316 78-79 -0.8725808067427003 2316 80-81 -0.5282472278376922 2316 82-83 -0.5815448975794908 2316 84-85 -0.1395850358620052 2316 86-87 -0.09158292311511929 2316 88-89 -0.18966470525267098 2316 90-91 -0.46974653355889373 2316 92-93 -0.7572744831556513 2316 94-95 -0.5765337679756612 2316 96-97 -0.48229242107249704 2316 98-99 -0.37262405428210954 2316 100-101 -0.5457315937519063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2184.0 3 641.0 4 656.0 5 679.0 6 712.0 7 857.0 8 1025.0 9 1402.0 10 1780.0 11 2297.0 12 3067.0 13 3932.0 14 4432.0 15 5604.0 16 7476.0 17 10067.0 18 8612.0 19 5196.0 20 4879.0 21 4931.0 22 5285.0 23 5907.0 24 6567.0 25 7502.0 26 8535.0 27 9890.0 28 11926.0 29 14204.0 30 17657.0 31 21483.0 32 27471.0 33 35247.0 34 48083.0 35 68042.0 36 98903.0 37 142661.0 38 129847.0 39 24201.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.131155026494355 19.50149196674135 14.995858597657453 21.371494409106838 2 14.879261693722404 21.384988214985228 40.530491650897986 23.20525844039438 3 18.708796971121043 26.755451477667062 30.780770017071962 23.754981534139937 4 12.102799268831832 17.399251827920423 38.86974472878762 31.62820417446013 5 12.804237442340154 38.142426621083345 34.15069855905178 14.902637377524714 6 29.384797632897204 37.24069971898623 18.28515195461282 15.089350693503748 7 26.492310838934685 34.587744357806635 21.682119363617165 17.23782543964151 8 26.020506405429778 33.259631384016366 20.80572830141574 19.914133909138112 9 26.871214536181064 16.764823079375198 21.31574752948677 35.04821485495697 10-11 24.003150340434658 27.154154843482402 27.711507441882393 21.131187374200543 12-13 25.93839093525046 25.259437689142196 28.13851549189917 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21.012787085634123 26.84617737875694 31.035475884109943 21.105559651498993 40-41 21.134954931396564 26.73293940477984 31.036508190399832 21.095597473423762 42-43 20.906363859595565 26.979273820505846 30.36766901824408 21.74669330165451 44-45 21.104762663162855 26.869529440650858 30.223036239241374 21.802671656944913 46-47 21.559413889471298 27.15817075339248 29.7805270323709 21.50188832476532 48-49 21.643245883060196 27.397937449322065 29.144642636752472 21.81417403086527 50-51 21.191560899266708 27.645279659155968 29.26710174435769 21.89605769721963 52-53 21.61217953184128 27.923304621668915 28.669064724332856 21.79545112215695 54-55 21.254630078155586 27.607342829500315 28.879411596861036 22.25861549548306 56-57 21.537352832269598 27.91279627646232 28.56875525065795 21.981095640610132 58-59 21.89208416341602 27.377465196253446 28.6979436955082 22.03250694482234 60-61 21.674723860020507 27.399921031763146 29.29144256387966 21.633912544336685 62-63 21.486991215984837 27.417462377922362 29.373757323330864 21.721789082761934 64-65 21.262146551523937 27.64686171924505 29.14296520172648 21.948026527504535 66-67 21.398539764246458 27.665965595990823 28.682263664075425 22.253230975687295 68-69 21.194521515177055 27.508024683574966 29.078586487740164 22.218867313507815 70-71 21.46850488513125 27.515691663863695 28.821843141079352 22.193960309925703 72-73 21.444616041883926 27.41627327511404 28.906849126047163 22.23226155695487 74-75 21.118838248934146 28.226787735479768 28.505110659071338 22.149263356514748 76-77 21.224550194892323 27.75234806428195 28.659722120842527 22.3633796199832 78-79 21.33716068228579 27.9363509656866 28.388597597398284 22.33789075462932 80-81 21.405626993537734 27.993566268561835 28.240006847783462 22.360799890116965 82-83 21.363840906466244 27.73875910441857 28.571978501160828 22.325421487954358 84-85 21.273426833809108 27.705605581621324 28.198085963711762 22.822881620857803 86-87 21.479800091591503 27.699593147893726 28.253257139823056 22.567349620691715 88-89 21.533954440625035 27.52469226775858 28.00798225104259 22.93337104057379 90-91 21.495944305467702 27.416508312829745 28.337759276061348 22.7497881056412 92-93 21.63263138680075 27.74577255604786 28.148620168931814 22.47297588821958 94-95 21.47086098386207 27.587754264825076 28.088135624723275 22.85324912658958 96-97 21.366281482268352 27.64570559784359 28.124657666121145 22.86335525376692 98-99 21.515182307907395 27.881330998155633 27.603181845653786 23.000304848283186 100-101 21.613876490047094 27.556000666781337 27.84330107236238 22.98682177080919 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 731.0 1 492.5 2 305.0 3 351.0 4 327.5 5 367.0 6 573.0 7 1096.5 8 1828.5 9 2145.5 10 1853.0 11 1603.5 12 1634.0 13 1637.0 14 1683.5 15 1785.0 16 1856.5 17 1890.5 18 1905.0 19 2004.0 20 2167.5 21 2423.5 22 2776.5 23 3188.0 24 3606.0 25 4191.5 26 5223.5 27 6305.5 28 7023.5 29 7770.0 30 8903.0 31 10427.5 32 12280.5 33 14136.5 34 16127.5 35 17420.5 36 19197.5 37 21278.5 38 21762.0 39 21873.0 40 22191.5 41 23082.5 42 23779.0 43 24892.0 44 26518.5 45 27145.0 46 27011.0 47 28422.0 48 33092.5 49 32724.5 50 27525.0 51 24851.0 52 23207.0 53 22490.5 54 21974.0 55 20352.0 56 18321.5 57 16393.5 58 14686.0 59 13143.0 60 11393.0 61 9334.5 62 7452.0 63 5682.5 64 4149.0 65 3149.5 66 2284.5 67 1677.0 68 1160.5 69 650.0 70 391.5 71 259.0 72 180.5 73 144.0 74 107.5 75 72.0 76 44.0 77 31.0 78 22.5 79 14.5 80 10.0 81 6.5 82 7.0 83 6.5 84 5.0 85 3.5 86 4.0 87 3.5 88 2.0 89 2.5 90 3.0 91 2.5 92 1.0 93 0.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06354074500528623 2 0.10240804831749678 3 0.0749488954655255 4 0.14313249240230444 5 0.1246937375886619 6 0.11394885169006444 7 0.1199182327448408 8 0.10797947063528807 9 0.12827536622152771 10-11 0.1210457824996319 12-13 0.10293865996681024 14-15 0.10778049126679552 16-17 0.11772945969142279 18-19 0.09265805926136204 20-21 0.07242849013128659 22-23 0.07780093308058532 24-25 0.06453564184774895 26-27 0.05657646710804712 28-29 0.07448461027237623 30-31 0.05239790036970367 32-33 0.05418871468613658 34-35 0.047224436788897484 36-37 0.05306116493134548 38-39 0.05193361517655439 40-41 0.06572951805870422 42-43 0.0599591163724204 44-45 0.05067341250943494 46-47 0.03568363341632984 48-49 0.041653014471106205 50-51 0.02500507397389656 52-53 0.04251525840124057 54-55 0.0464285193149273 56-57 0.049081577561494574 58-59 0.05412238822997239 60-61 0.05034178022861403 62-63 0.05743871103818149 64-65 0.0384030181190613 66-67 0.06307645981213694 68-69 0.05180096226422603 70-71 0.02420915649992638 72-73 0.01923467228761274 74-75 0.0299795581862102 76-77 0.04695913096424075 78-79 0.06592849742719677 80-81 0.042249952576583844 82-83 0.0668570678134953 84-85 0.06864788212992821 86-87 0.0665917619888386 88-89 0.08967336873397387 90-91 0.0694437996038984 92-93 0.0775356272559286 94-95 0.08244378501207804 96-97 0.09876009322846678 98-99 0.13444372664479662 100-101 0.12953556888864717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 753847.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.523355577189704 #Duplication Level Percentage of deduplicated Percentage of total 1 80.48568450879509 47.10292333381724 2 11.195565158823033 13.104040813547934 3 3.4337415349038487 6.028622304220293 4 1.5031549251806882 3.5187868069581367 5 0.8112962333773116 2.3739888972187546 6 0.4953617185136541 1.73941379951414 7 0.34768052615821 1.424320174173493 8 0.24540944985144128 1.1489747596526723 9 0.2006573178123178 1.0568825603545893 >10 1.1623086368732258 12.603704845137969 >50 0.08251663995615237 3.25814703156094 >100 0.03300622879263166 3.288542884486092 >500 0.002260700601515073 0.966479150564823 >1k 0.0011303503007575365 1.5721658790091433 >5k 2.2607006015150732E-4 0.8130067597838245 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6145 0.815152146257795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4244 0.5629789599215755 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2860 0.3793873292591202 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2043 0.2710098998868471 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1489 0.19752018645693356 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1247 0.16541818167346956 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 994 0.13185699485439353 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 971 0.12880597787084117 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 828 0.10983661140788516 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.632645616418186E-5 0.0 18-19 0.0 0.0 0.0 1.326529123283637E-4 0.0 20-21 0.0 0.0 0.0 1.9897936849254557E-4 0.0 22-23 0.0 0.0 0.0 5.969381054776367E-4 0.0 24-25 0.0 0.0 0.0 0.001326529123283637 0.0 26-27 0.0 0.0 0.0 0.0017244878602687282 0.0 28-29 0.0 0.0 0.0 0.00391326091368673 0.0 30-31 0.0 0.0 0.0 0.008688765757507823 0.0 32-33 0.0 0.0 0.0 0.01359692351365728 0.0 34-35 0.0 0.0 0.0 0.018040796076657463 0.0 36-37 0.0 0.0 0.0 0.023811197762941286 0.0 38-39 0.0 0.0 0.0 0.03263261643277747 0.0 40-41 0.0 0.0 0.0 0.05073973896559912 0.0 42-43 0.0 0.0 0.0 0.08164786753810788 0.0 44-45 0.0 0.0 0.0 0.11156109926815388 0.0 46-47 0.0 0.0 0.0 0.18405591585560466 0.0 48-49 0.0 0.0 0.0 0.24918849580883123 0.0 50-51 0.0 0.0 0.0 0.2956833415799227 0.0 52-53 0.0 0.0 0.0 0.36021898342767167 0.0 54-55 0.0 0.0 0.0 0.4211729966425548 0.0 56-57 0.0 0.0 0.0 0.552167747566814 0.0 58-59 0.0 0.0 0.0 0.7250144923306718 0.0 60-61 0.0 0.0 0.0 0.9046265356232763 0.0 62-63 0.0 0.0 0.0 1.088284492741896 0.0 64-65 0.0 0.0 0.0 1.301723028678233 0.0 66-67 0.0 0.0 0.0 1.5629166130527814 0.0 68-69 0.0 0.0 0.0 1.8571407725970919 0.0 70-71 0.0 0.0 0.0 2.1078547768976996 0.0 72-73 0.0 0.0 0.0 2.3218902509395143 0.0 74-75 0.0 0.0 0.0 2.5528389713031956 0.0 76-77 0.0 6.632645616418186E-5 0.0 2.831410087192759 0.0 78-79 0.0 1.326529123283637E-4 0.0 3.107261818379592 0.0 80-81 0.0 1.326529123283637E-4 0.0 3.445593071273083 0.0 82-83 0.0 1.326529123283637E-4 0.0 3.771587603320037 0.0 84-85 0.0 1.326529123283637E-4 0.0 4.2342146350652055 0.0 86-87 0.0 1.326529123283637E-4 0.0 4.735708970122585 0.0 88-89 0.0 1.326529123283637E-4 0.0 5.382192938354866 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7960 0.0 27.760902 1 GTACATG 4800 0.0 24.65198 1 TATCAAC 9305 0.0 23.800829 2 CAACGCA 9295 0.0 23.76899 5 TCAACGC 9355 0.0 23.670475 4 TACATGG 4985 0.0 23.643353 2 GAGTACT 3985 0.0 23.607176 12-13 ATCAACG 9410 0.0 23.582623 3 AACGCAG 9420 0.0 23.554459 6 ACATGGG 4885 0.0 23.054125 3 GGTATCA 5660 0.0 22.50153 1 GTACTTT 4280 0.0 21.980776 14-15 AGTACTT 4085 0.0 21.691715 12-13 CTACGAG 110 0.00286988 21.599424 3 ACGCAGA 10325 0.0 21.21378 7 CGCAGAG 10320 0.0 21.085938 8 AGAGTAC 7050 0.0 20.82321 10-11 TATAGGT 160 4.9712307E-5 20.789448 3 GTACTAT 115 0.0037121791 20.661692 1 CATGGGA 3290 0.0 20.65404 4 >>END_MODULE