FastQCFastQC Report
Thu 26 May 2016
SRR937123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753847
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67960.9015091921835598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52800.7004073770937604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36690.48670353533276645No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26480.35126491184550707No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14160.187836523856963No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12010.15931614770636482No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.1491018734570808No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10390.1378263759091699No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10350.13729576425985643No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9620.12761210165988587No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA250.0050205857.000082
CGCAGTA500.001615978737.997538
GTATCAA89950.025.2485351
GAGTACT50200.023.60653312-13
CCCTATA1252.2548811E-422.8000342
AGTACTT51150.022.61094712-13
GTACATG43450.022.3074461
GGTATCA66350.022.2704471
GTACTTT54350.021.89285514-15
GGTATGC1754.0484665E-621.7128758
ACCGTGC1100.002877541121.5895088
ATCAACG105650.021.3545063
TCAACGC106000.021.3288024
TACATGG45900.021.2146262
CAACGCA106500.021.184075
TATCAAC106550.021.1309552
GTATGGT2056.8053487E-720.8592221
AACGCAG108700.020.8427126
AGAGTAC81000.020.7872310-11
ACATGGG44600.020.6600933
Produced by FastQC (version 0.11.5)